Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,985,726	A→G	intergenic (‑134/‑121)	yqeF ← / → yqeG	putative acyltransferase/putative transporter YqeG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,985,726	0	A	G	100.0%	271.6 / NA	72	intergenic (‑134/‑121)	yqeF/yqeG	putative acyltransferase/putative transporter YqeG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (45/27);  total (45/27)

TTAACGCTTTCACGACCAGACTACCAAGTTCCACGGCGGAATGACCCGCTAACGCACCACGAAAGCAGCCGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTT  >  NC_000913/2985481‑2985969                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    ttAACGCTTTCACGACCAGACTACCAAGTTCCACGAAGGAATGACCCGCTAACGCACCACGAAAGCAGACGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGAAGTCAGAGACATAACTGGCACGTAGGGttt                                                                                                                                                                                                                                                >  1:542243/1‑251 (MQ=255)    aCGCTTTCACGACCAGACTACCAAGTTCCACGGCGGAATGACCCGCTAACGCACCACGAAAGCAGCCGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGc                                                                                                                                                                                                                                              >  1:386717/1‑250 (MQ=255)      gCTTTCACGACCAGACTACCAAGTTCCACGGCGGAATGACCCGCTAACGCACCACGAAAGCAGCCGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAAc                                                                                                                                                                                                                                           >  1:24939/1‑251 (MQ=255)                        ccAAGTTCCACGGCGGAATGACCCGCTAACGCACCACGAAAGCAGCCGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATaga                                                                                                                                                                                                                         <  1:591786/251‑1 (MQ=255)                            gTTCCACGGCGGAATGACCCGCTAACGCACCACGAAAGCAGCCGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAgggg                                                                                                                                                                                                                     <  1:157723/251‑1 (MQ=255)                               ccACGGCGGAATGACCCGCTAACGCACCACGAAAGCAGCCGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGt                                                                                                                                                                                                                    >  1:600451/1‑249 (MQ=255)                               ccACGGCGGAATGACCCGCTAACGCACCACGAAAGCAGCCGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAg                                                                                                                                                                                                                  <  2:110266/251‑1 (MQ=255)                                     gggAATGACCCGCTAACGCACCACGAAAGCAGCCGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAggg                                                                                                                                                                                                            <  2:54028/250‑1 (MQ=255)                                        aaTGACCCGCTAACGCACCACGAAAGCAGCCGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGt                                                                                                                                                                                                         >  2:411872/1‑251 (MQ=255)                                                    aCGCACCACGAAAGCAGCCGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCAt                                                                                                                                                                                              >  1:568396/1‑250 (MQ=255)                                                             gAAAGCAGCCGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTg                                                                                                                                                                                    >  1:271562/1‑251 (MQ=255)                                                               aaGCAGCCGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGt                                                                                                                                                                                  <  1:123624/251‑1 (MQ=255)                                                                agcagcCGATAGGTGTCCGTAACGTCCCGACAATCACAACGTCATTCATCACACCCGCGCACCAAGTAACAGTTCAATAGTAAATGATTGTTAATTACAGTTATCTTAAGTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATATCTGGCACTTAGGGTTTGCAACCACTTACCTACCAATTGAGGGGTAAATAGGGCGTTAATCTCCCATACTTAAGCTGGtt                                                                                                                                                                                 <  2:483820/251‑1 (MQ=255)                                                                agcagcCGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTCACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGtt                                                                                                                                                                                 >  2:570084/1‑251 (MQ=255)                                                                agcagcCGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGtt                                                                                                                                                                                 >  2:9603/1‑251 (MQ=255)                                                                   cgcCGATAGGTGACCGTAACTCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAAATACAGTTATCTTAATTGTTTAAAAAAACTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATTACTGGCACGTAGTGTTTTCAACCACTATCCCACCAATAGAGAGGTAGATGGGGCGTTAAACGCCCAGACTTAACCGTGTTTAt                                                                                                                                                                              <  2:159591/250‑1 (MQ=255)                                                                      cGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATATTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGCAAATATGCCTGCGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCAACCAATCGAGGGGTAGATAGGGCGTTACTCTCCCATACTTAACCTGGTTTATGGt                                                                                                                                                                           >  2:177092/1‑251 (MQ=255)                                                                      cGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGt                                                                                                                                                                           <  1:411872/251‑1 (MQ=255)                                                                       gATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGt                                                                                                                                                                           >  2:420789/1‑250 (MQ=255)                                                                       gATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGt                                                                                                                                                                           >  2:480619/1‑250 (MQ=255)                                                                       gATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGAGCAATAGTACATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGt                                                                                                                                                                           >  1:574677/1‑250 (MQ=255)                                                                                       gacccGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCAttct                                                                                                                                                          >  2:459036/3‑249 (MQ=255)                                                                                         cccGACAATCACAACGTCTTTCAGCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTTATTTTTATCACAAAGGAAATATGCCTGAGCTGCAGTCAGAGACATAACGGGCGCGTAGGGTTTGCAACCGCTATCCCACCAAGAGTGGGGTAGATAGGGGGTTAATCTCCGTTACTTAACCTGGTGTATGGTAAAGTGCCCTCCATTCTGt                                                                                                                                                        <  1:221246/251‑1 (MQ=255)                                                                                          ccGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGtt                                                                                                                                                       <  2:335368/251‑1 (MQ=255)                                                                                                  cacaACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGt                                                                                                                                               >  1:70195/1‑251 (MQ=255)                                                                                                            ttCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATAc                                                                                                                                      <  2:53019/250‑1 (MQ=255)                                                                                                                 cacaACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCt                                                                                                                                 <  1:560930‑M1/250‑2 (MQ=255)                                                                                                                 cacaACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCt                                                                                                                                 >  1:207765‑M1/1‑249 (MQ=255)                                                                                                                  acaACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaa                                                                                                                               <  1:52110‑M1/251‑4 (MQ=255)                                                                                                                              cAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACGTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgac                                                                                                                   <  2:451355‑M1/251‑16 (MQ=255)                                                                                                                               aaTTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgaca                                                                                                                  <  1:480619‑M1/251‑17 (MQ=255)                                                                                                                               aaTTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgac                                                                                                                   >  1:573768‑M1/1‑235 (MQ=255)                                                                                                                                     cAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgcc                                                                                                            <  1:420237‑M1/251‑23 (MQ=255)                                                                                                                                     cAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGCAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgcc                                                                                                            >  1:266981‑M1/1‑229 (MQ=255)                                                                                                                                         gCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATCGCGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccgggg                                                                                                        >  1:595888‑M1/1‑225 (MQ=255)                                                                                                                                          cAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggt                                                                                                       <  2:623502‑M1/251‑28 (MQ=255)                                                                                                                                          aaatAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAATGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggt                                                                                                       <  2:271562‑M1/250‑28 (MQ=255)                                                                                                                                           aaTAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggtt                                                                                                      >  2:158777‑M1/1‑223 (MQ=255)                                                                                                                                              aGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtg                                                                                                   >  1:15920‑M1/1‑220 (MQ=255)                                                                                                                                              aGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGAAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtg                                                                                                   >  2:638247‑M1/1‑220 (MQ=255)                                                                                                                                                 aaaTGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagc                                                                                                >  1:491286‑M1/1‑217 (MQ=255)                                                                                                                                                  aaTGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagc                                                                                                >  2:601374‑M1/1‑216 (MQ=255)                                                                                                                                                  aaTGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagc                                                                                                >  2:622964‑M1/1‑216 (MQ=255)                                                                                                                                                   aTGATTGGTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcat                                                                                              <  2:541922‑M1/251‑37 (MQ=255)                                                                                                                                                           tAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaat                                                                                      >  2:441510‑M1/1‑207 (MQ=255)                                                                                                                                                                aCAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgac                                                                                                                   >  2:182280‑M1/1‑202 (MQ=255)                                                                                                                                                                aCAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgac                                                                                                                   <  1:182280‑M1/217‑16 (MQ=255)                                                                                                                                                                 cAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCCGTCAGAGACATAACGTGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacagttgccggggttgtgagcataacgtaatgcttat                                                                                <  2:72656‑M1/251‑51 (MQ=255)                                                                                                                                                                    ttATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttatttt                                                                             >  1:136791‑M1/1‑198 (MQ=255)                                                                                                                                                                       tCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttacc                                                                          >  1:476591‑M1/1‑195 (MQ=255)                                                                                                                                                                          tAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccgg                                                                        >  1:581262‑M1/1‑192 (MQ=255)                                                                                                                                                                                     aaaaaGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgg                                                            >  2:557‑M1/1‑181 (MQ=255)                                                                                                                                                                                         aGTGATTTTTATAACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaacaaaaccccgacatttgccggggttgtgagcataacggaatgcttattttaccggacgcatcgccgggaac                                                        >  1:283768‑M1/1‑177 (MQ=255)                                                                                                                                                                                                   aTCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgt                                              >  2:589846‑M1/1‑167 (MQ=255)                                                                                                                                                                                                     cacaAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcg                                            >  2:486249‑M1/1‑165 (MQ=255)                                                                                                                                                                                                           ggcaatatGCCTGAGCAGCAGTCAGAGACATAACTGGCACGCAGGGATTGCAACCACTAACCCACCCATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaagaaaaccccgacattagccggggttgtgagcataacgtagtgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatg                                      >  2:614504‑M1/4‑159 (MQ=255)                                                                                                                                                                                                            gAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatgg                                     >  2:313035‑M1/1‑158 (MQ=255)                                                                                                                                                                                                            gAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatgg                                     >  1:628096‑M1/1‑158 (MQ=255)                                                                                                                                                                                                             aaaTATGCTTCAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTAAACCTGGTTTATGGTAATTTGACCTCACTTCTGTTTAATTTGTAGATGATACGATCtaaaaaaaccccgacatttgccggggttgtgagaataacgtaatgcttattttacagtacgcatcgccgggaacagaataacgacgcggatggt                                    >  2:193303‑M1/1‑157 (MQ=255)                                                                                                                                                                                                             aaaTATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaccagactaacgtcgcggatggt                                    <  1:601374‑M1/251‑95 (MQ=255)                                                                                                                                                                                                              aaTATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggcacagaataacgtagcggatggt                                    >  2:267078‑M1/1‑156 (MQ=255)                                                                                                                                                                                                                tatGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatg                                 >  2:93536‑M1/1‑154 (MQ=255)                                                                                                                                                                                                                  tGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTACCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTCATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggc                               >  1:319554‑M1/1‑152 (MQ=255)                                                                                                                                                                                                                   gCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggct                              >  2:235410‑M1/1‑151 (MQ=255)                                                                                                                                                                                                                        agcagcAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctgttgg                         >  2:29271‑M1/1‑146 (MQ=255)                                                                                                                                                                                                                            gcagTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCCATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctgttggtga                      >  1:258400‑M1/1‑142 (MQ=255)                                                                                                                                                                                                                             cagTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctgttggtgaac                    <  1:158777‑M1/251‑111 (MQ=255)                                                                                                                                                                                                                             cagTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctgttggtgaac                    <  1:402151‑M1/251‑111 (MQ=255)                                                                                                                                                                                                                                 cAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctgttggtgaacagca                <  1:371701‑M1/251‑115 (MQ=255)                                                                                                                                                                                                                                             gggcACGTAGGGTTTGCAACCACTAACACACCAATAGAGGGGTAGATAGGGCGTTAATCTACCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattgtaccggacgcagcgccgggaacagaataacgtcgcggatggtatggctgttggtgaacagcattaccatacggn    <  2:407464‑M1/250‑127 (MQ=255)                                                                                                                                                                                                                                               gaacgTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTCTGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctggtggtgaacagcaataccatacggtcc  >  2:497609‑M1/3‑123 (MQ=255)                                                                                                                                                                                                                                               gCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctgttggtgaacagcattaccatactgtcg  <  1:387689‑M1/251‑129 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    TTAACGCTTTCACGACCAGACTACCAAGTTCCACGGCGGAATGACCCGCTAACGCACCACGAAAGCAGCCGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTT  >  NC_000913/2985481‑2985969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑