Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,113,177	A→G	intergenic (‑116/+257)	rfbB ← / ← galF	dTDP‑glucose 4,6‑dehydratase 1/UTP:glucose‑1‑phosphate uridylyltransferase, low activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,113,177	0	A	G	100.0%	227.5 / NA	59	intergenic (‑116/+257)	rfbB/galF	dTDP‑glucose 4,6‑dehydratase 1/UTP:glucose‑1‑phosphate uridylyltransferase, low activity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/31);  total (28/31)

TGATTCCCGGTTTCCGGCGTACGTTAATTTATCGACATTAACAACACTATCCTGCGTATTATTTATAATGTGACGAACTACAGCTGAACCAATAAATCCTGCGCCACCAGTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTC  >  NC_000913/2112936‑2113402                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                  gacattacatactcctgtcaggtgtgatcaccgcgctgaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCt                                                                                                                                                                                                                          >  1:105585‑M2/132‑251 (MQ=255)        catactcctgtcaggtttgatccccgcgctgaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGAtgt                                                                                                                                                                                                                   <  1:274876‑M2/127‑1 (MQ=255)         atactcctgtcaggtgtgatcaccgcgctgaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGAtgtg                                                                                                                                                                                                                  >  2:366182‑M2/124‑251 (MQ=255)                           atcaccgctcttaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttatcaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATTCACGCTACCGCCCCTTGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTCCCCACTc                                                                                                                                                                                                 <  1:568970‑M2/145‑1 (MQ=255)                                cgcgctgaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTa                                                                                                                                                                                           <  2:105585‑M2/151‑1 (MQ=255)                                  cgctgaatgctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTaa                                                                                                                                                                                          >  1:390799‑M2/99‑250 (MQ=255)                                          gctcgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGaaaa                                                                                                                                                                                 <  1:43734‑M2/161‑1 (MQ=255)                                             cgcggcctggaatgttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGaaaaa                                                                                                                                                                                <  1:366182‑M2/162‑1 (MQ=255)                                                          gttcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAAtt                                                                                                                                                                  <  1:301157‑M2/176‑1 (MQ=255)                                                            tcgcaaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGt                                                                                                                                                                <  2:279453‑M2/178‑1 (MQ=255)                                                               caaataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAACAATTCAAACGCTAATTGTCtt                                                                                                                                                             >  2:315732‑M2/70‑250 (MQ=255)                                                                 aataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTAcc                                                                                                                                                          >  2:72107‑M2/68‑251 (MQ=255)                                                                 aataagtatacaaagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGAATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTAcc                                                                                                                                                          <  2:443311‑M2/184‑1 (MQ=255)                                                                        atacaaagtccttattaaagtgtaaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCg                                                                                                                                                    <  1:245200‑M2/190‑1 (MQ=255)                                                                             aagtacttattaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTgg                                                                                                                                              >  1:75720‑M2/56‑251 (MQ=255)                                                                                     attaaagtataaatagcttatccatgcttatatgcttacggctttatTTTTTCCATATTACTTCAGAGCATGCTGTTAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCTGTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAgg                                                                                                                                      <  2:528148‑M2/204‑1 (MQ=255)                                                                                      ttaaagtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAgg                                                                                                                                      >  1:463664‑M2/47‑250 (MQ=255)                                                                                          agtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAAtt                                                                                                                                                                              >  1:529983‑M2/43‑206 (MQ=255)                                                                                          agtataaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTGCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAAtt                                                                                                                                                                              <  2:529983‑M2/164‑1 (MQ=255)                                                                                               aaatagcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTTTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTTCCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTgg                                                                                                                            <  2:542052‑M2/214‑1 (MQ=255)                                                                                                   agcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGaaa                                                                                                                         >  2:446322‑M2/34‑250 (MQ=255)                                                                                                   agcttatccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGaaa                                                                                                                         >  1:161161‑M2/34‑250 (MQ=255)                                                                                                          ccatgcttatatgcttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGa                                                                                                                  >  2:511113‑M2/27‑250 (MQ=255)                                                                                                                    atgctgacggttttatTTATTCCTTATTCCTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCt                                                                                                        <  2:58952‑M2/234‑1 (MQ=255)                                                                                                                     tgcttccggctttatTTATTCCATATTACTTCAGAGCCTGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAACTTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGCCAACTTTGAATAAAACCCTAc                                                                                                      >  2:428041‑M2/16‑251 (MQ=255)                                                                                                                       cttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTg                                                                                                    >  2:63167‑M2/14‑251 (MQ=255)                                                                                                                       cttacggctttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTg                                                                                                    >  1:157672‑M2/14‑251 (MQ=255)                                                                                                                               tttatTTATTACATATTACTTCAGAGCATGATGTGAAATAAGCTGATCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACAAGAGCACTGCGTGCATGCCTATGACGTGACGAGCGTTACCCACTCGCGCTAAACCAGAAAAATTAAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAACACTTTGAATAAAACCCTACTGCTAACTCg                                                                                            >  2:178040‑M2/6‑251 (MQ=255)                                                                                                                                ttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGt                                                                                                                                                                                                          >  2:542328‑M2/5‑140 (MQ=255)                                                                                                                                ttatTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGt                                                                                                                                                                                                          <  1:542328‑M2/136‑1 (MQ=255)                                                                                                                                  aTTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAATCCCTACTGCGAACTCgtt                                                                                          <  1:315732/250‑1 (MQ=255)                                                                                                                                  aTTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCgtt                                                                                          <  2:161161/250‑1 (MQ=255)                                                                                                                                     tATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCgttgtt                                                                                       <  1:29166/250‑1 (MQ=255)                                                                                                                                      aTTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTAt                                                                                     <  1:302886/251‑1 (MQ=255)                                                                                                                                         ccATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAGACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCt                                                                                  >  2:113256/1‑251 (MQ=255)                                                                                                                                         ccATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCt                                                                                  >  1:90529/1‑251 (MQ=255)                                                                                                                                         ccATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCt                                                                                  <  1:597868/251‑1 (MQ=255)                                                                                                                                              ttACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATgg                                                                             >  2:377722/1‑251 (MQ=255)                                                                                                                                                aCTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGtt                                                                           >  1:47663/1‑251 (MQ=255)                                                                                                                                                   tCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTtat                                                                        >  1:190395/1‑251 (MQ=255)                                                                                                                                                    cAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTtat                                                                        <  1:377722/250‑1 (MQ=255)                                                                                                                                                     agagCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAAc                                                                                               >  1:641358/1‑226 (MQ=255)                                                                                                                                                     agagCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAAc                                                                                               <  2:641358/226‑1 (MQ=255)                                                                                                                                                          aTGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAaac                                                                 >  1:613986/1‑251 (MQ=255)                                                                                                                                                           tGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAaac                                                                 >  1:486409/1‑250 (MQ=255)                                                                                                                                                             cggtgAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCGACCGCCCCTGGCTTTACAGCTGCCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTTGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAaacaac                                                              <  1:409652/249‑1 (MQ=255)                                                                                                                                                               gtgAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACGCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACgg                                                            <  1:484180/251‑1 (MQ=255)                                                                                                                                                                 gAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGc                                                           <  1:511113/250‑1 (MQ=255)                                                                                                                                                                          ggCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTc                                                 >  1:85363/1‑251 (MQ=255)                                                                                                                                                                                 aGTTTGATTAAGAGAGGTATTAATGCCCGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGcaacaa                                          <  2:390799/251‑1 (MQ=255)                                                                                                                                                                                  gTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACaaa                                         <  2:179599/251‑1 (MQ=255)                                                                                                                                                                                        ttAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACtt                                   >  1:248196/1‑251 (MQ=255)                                                                                                                                                                                         tAATAGAGGAATTAATGCACGCTACCGCCCATGGCTTTACAGCTACCAGAGAACTGCGTGCATGCCTATGCTGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGCCAGGAAGATTCTCAACAAATTACttt                                  >  2:331564/1‑251 (MQ=255)                                                                                                                                                                                               gggTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTtgctgc                            <  1:72107/250‑1 (MQ=255)                                                                                                                                                                                                    ttAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAAt                        <  1:365582/250‑1 (MQ=255)                                                                                                                                                                                                                aCCGCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGt            <  2:486409/250‑1 (MQ=255)                                                                                                                                                                                                                   gCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTTTTGCGAATTTTCACTGCCGtttt         >  2:100423/1‑248 (MQ=255)                                                                                                                                                                                                                   gCCCCTGGCTTTACAGCTACCAGAGCACTGCGTGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTtata        <  2:75720/251‑1 (MQ=255)                                                                                                                                                                                                                          gctgtACAGCTACCAGATCCCTGCGTGCATGCCTATGATGTTACGAGCGATACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTc  <  2:450665/246‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                  TGATTCCCGGTTTCCGGCGTACGTTAATTTATCGACATTAACAACACTATCCTGCGTATTATTTATAATGTGACGAACTACAGCTGAACCAATAAATCCTGCGCCACCAGTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGCGGCTCTCAGTTTGATTAATAGAGGTATTAATGCACGCTACCGCCCCTGGCTTTACAGCTACCAGAGCACTGCATGCATGCCTATGATGTGACGAGCGTTACCCACTCGCGCTAAACCCGAAAAATTCAAACGCTAATTGTCTTACCAATCCGCTCTGGAAACAAGGAAAATCCTGGAAAACTTTGAATAAAACCCTACTGCTAACTCGTTGTTATTCTGATGGTTTATATAAAACAACGGCAGGAAGATTCGCAACAAATTACTTTTGCTGCGAATTTTCACTGCCGTTATAATTTTC  >  NC_000913/2112936‑2113402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑