Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,041,467	A→G	K31K (AAA→AAG)	zinT →	metal‑binding protein ZinT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,041,467	0	A	G	100.0%	199.3 / NA	57	K31K (AAA→AAG)	zinT	metal‑binding protein ZinT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/35);  total (22/35)

CCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCAT  >  NC_000913/2041222‑2041703                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             cgcggtcttgctgggtccgggactgggtggagaaacactgtcgacgattgaatggctggcgctcggcgtaattgtcttcgcggtggtactggtcacgttgggaaaatatctcttcccggcaacacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCtt                                                                                                                                                                                                                                         >  2:552085‑M2/158‑251 (MQ=255)   cggtcttgctgggtacgggactgggtggagaaacactgtcgaagattgaatggctggcgctcggcgtaattgtcttcgcggtggtactggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTa                                                                                                                                                                                                                                        >  1:101728‑M2/156‑250 (MQ=255)     gtcttgctgggtacgggactgggtggagaaacactgtcgaagattgaatggctggcgctcggcgtaattgtcttcgcggtggtactggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACa                                                                                                                                                                                                                                     <  1:616559‑M2/98‑1 (MQ=255)                    ggactgggtggagaaacactgtcgaagattgaatggctggcgctcggcgtaattgtcttcgcggtggtactggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACaaaa                                                                                                                                                                                                                       >  1:404619‑M2/139‑250 (MQ=255)                         gggtggagaaacactgtcgaagattgaatggctggcgctcggcgtaattgtcttcgcggtggtactggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAgct                                                                                                                                                                                                                   <  2:400168‑M2/116‑1 (MQ=255)                          ggtggagaaacactgtcgaagattgaatggctggcgctcggcgtaattgtcttcgcggtggtactggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGAGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGGACAAAAAgctgct                                                                                                                                                                                                                >  2:133168‑M2/133‑251 (MQ=255)                           gtggagaaacactgtcgaagattgaatggctggcgctcggcgtaattgtcttcgcggtggtactggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAgctgct                                                                                                                                                                                                                >  1:544077‑M2/132‑250 (MQ=255)                                  aacactgtcgaagattgaatggctggcgctcggcgtaattgtcttcgcggtggtactggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGgtgt                                                                                                                                                                                                        >  1:15606‑M2/125‑251 (MQ=255)                                   acactgtcgaagattgaatggctggcgctcgtcgtaattgtcttcgcgggtgtactggtcacgttgtgaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGtt                                                                                                                                                                                                       <  2:341028‑M2/128‑1 (MQ=255)                                        gtcgaagattgaatggctggcgctaggcgtaattgtcttcgcggcggaactggtcacgttgggaaaatatctcttcccggcaaaacccgtggttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCGCCTGCCTTTTCGCATGGTCCTCACTCACACGGCAAGCCATTAGCAGAGGTCGAACAAAAAGCTGCTAAAGGGGTTTTtg                                                                                                                                                                                                   >  2:621697‑M2/119‑250 (MQ=255)                                         tcgaagattgaatggctggcgctcggcgtaattgtcttcgcggtggtactggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGATGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACGCACACGGCAAGCCCTTAACATAGGTCGCACAAAAAGCTGCTAATGGTGTTTTtgat                                                                                                                                                                                                 >  1:290668‑M2/118‑251 (MQ=255)                                          cgaagattgaatggctggcgctcggcgtaattgtcttcgcggtggtactggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTtgat                                                                                                                                                                                                 <  2:401788‑M2/134‑1 (MQ=255)                                            aagattgaatggctggcgctcggcgtaattgtcttcgcggtggtactggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTgatgat                                                                                                                                                                                              >  2:421167‑M2/115‑251 (MQ=255)                                             agattgaatggctggcgctcggcgtaattgtcttcgcggtggtactggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGatgatg                                                                                                                                                                                             <  2:70640‑M2/138‑1 (MQ=255)                                                  gaatggctggcgctcggcgtaattgtcttcgcggtggtactggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCaa                                                                                                                                                                                         <  1:421167‑M2/142‑1 (MQ=255)                                                  gaatggctggcgctcggcgtaattgtcgtcgcggtggtactggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcagggcgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTCGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGTTGTTTTTGATGATGCCaa                                                                                                                                                                                         <  2:490937‑M2/142‑1 (MQ=255)                                                          ggtgctcggcgtaattgtcttcgctgtggttctggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccaggtattcaggacgcatcaCGATTCGTCTTTACAAACTTGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACaaa                                                                                                                                                                                 <  2:132121‑M2/150‑1 (MQ=255)                                                             gctcggcgtaattgtcttcgcggtggtactggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCg                                                                                                                                                                             <  2:450186‑M2/154‑1 (MQ=255)                                                                cggcgtaattgtcttcgcggtggtactggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAAc                                                                                                                                                                          >  1:459695‑M2/95‑251 (MQ=255)                                                                           tcttcgcggtggtactggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACt                                                                                                                                                               >  1:274132‑M2/84‑251 (MQ=255)                                                                                        actggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcagtacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCt                                                                                                                                                   <  1:226563‑M2/180‑1 (MQ=255)                                                                                        actggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCt                                                                                                                                                   <  2:285740‑M2/180‑1 (MQ=255)                                                                                        actggtcacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTg                                                                                                                                                  <  1:537219‑M2/181‑1 (MQ=255)                                                                                              cacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATc                                                                                                                                            >  1:496438‑M2/65‑251 (MQ=255)                                                                                              cacgttgggaaaatatctcttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATc                                                                                                                                            <  2:557709‑M2/187‑1 (MQ=255)                                                                                                         aatatctcttcccggcaaaacccgtagttgcgccagttagtcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCGCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTGTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGTAGTCTGGCTATCCGTTTAACCtt                                                                                                                                 <  2:553466‑M2/198‑1 (MQ=255)                                                                                                                 ttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCt                                                                                                                                  >  1:461744‑M2/46‑242 (MQ=255)                                                                                                                 ttcccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCt                                                                                                                                  <  2:461744‑M2/197‑1 (MQ=255)                                                                                                                    ccggcaaatcccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCGTTACTGCAAAGt                                                                                                                      <  2:168158‑M2/209‑1 (MQ=255)                                                                                                                    ccggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGt                                                                                                                      >  2:334125‑M2/43‑251 (MQ=255)                                                                                                                     cggcaaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTG‑TTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTgg                                                                                                                    <  2:101728‑M2/210‑1 (MQ=255)                                                                                                                         aaaacccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCa                                                                                                                  >  2:108328‑M2/38‑250 (MQ=255)                                                                                                                           atcccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCGTTATTGT‑TAGCGCTCCTGCCCTTTCGCATGGTCATCACTCACACGGCAAGCACTTAACAGAGGTCGAATAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTATTGCAAAGTTGcaaat                                                                                                               <  2:393245‑M2/216‑2 (MQ=255)                                                                                                                              ccgtagttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTg                                                                                                            <  1:236694‑M2/219‑1 (MQ=255)                                                                                                                                  agttgcgccagttattcaggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGAcc                                                                                                         <  1:191166‑M2/222‑1 (MQ=255)                                                                                                                                        gccgtttattcagtacgcatcaCGATTCGTCTTTATAAACTGGCTGTTGCTTTAGTTGTCTTTATTGT‑TAGCGCTCCTTCCTTTTCGCATGGTCATCACTCACACGTCAAGCCCTTAACAGAGGTCGAACAACAAGCTGCTAATGGTGTTTGTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCGATCCGTTTATCCTTTACTGCAAAATGGCAAACTTGACCCCGTCtt                                                                                                  <  2:254638‑M2/229‑1 (MQ=255)                                                                                                                                                  caggacgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTTTCTTTATTGT‑TAGCTCTCCTGCCTTTTCGCATGGTCATCACTCACACGTCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGTTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAaaagaa                                                                                         <  1:621697‑M2/238‑1 (MQ=255)                                                                                                                                                      acgcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCgg                                                                                    <  1:193703‑M2/243‑1 (MQ=255)                                                                                                                                                        gcatcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGAt                                                                                  <  1:244274‑M2/245‑1 (MQ=255)                                                                                                                                                         catcaCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATg                                                                                 >  1:598221‑M2/6‑251 (MQ=255)                                                                                                                                                          atcaCGATTCGTCTTTCCAAACTGGCTGTTGCTTTAGGTGGCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGc                                                                                <  2:496438‑M2/247‑1 (MQ=255)                                                                                                                                                               gATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGAt                                                                            >  2:167425/1‑250 (MQ=255)                                                                                                                                                                   cGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGAtaaaa                                                                        >  2:418033/1‑250 (MQ=255)                                                                                                                                                                              aCTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTg                                                            <  1:56242/251‑1 (MQ=255)                                                                                                                                                                                  gCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGa                                                        <  1:138859/251‑1 (MQ=255)                                                                                                                                                                                     gTTGCTATAGGTGTCTTTAT‑GTGTAGCGCTCCTGCCTGTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTGATGGTGTTTTTGATGATGCCTATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTAGCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGaaa                                                      <  2:7809/250‑1 (MQ=255)                                                                                                                                                                                      ttGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAAtt                                                    >  1:238894/1‑251 (MQ=255)                                                                                                                                                                                             aGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGat                                              <  1:437965/250‑1 (MQ=255)                                                                                                                                                                                              ggTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGatta                                            <  2:147184/251‑1 (MQ=255)                                                                                                                                                                                                       aTTGT‑TAGCGCTACTGCCTTTTCGCATGGTCATCACTAACACGGCAAGCCCTTAACAGAGGCCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGCGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGAGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATcaca                                    >  2:634909/1‑250 (MQ=255)                                                                                                                                                                                                         tGT‑TAGCGCTCCTGCCTTTTCGCATGGTCACCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACaaa                                  <  2:404619/250‑1 (MQ=255)                                                                                                                                                                                                               gcgcTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGCACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTAt                            >  2:166907/1‑251 (MQ=255)                                                                                                                                                                                                                              cGCATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCgag             <  1:334125/251‑1 (MQ=255)                                                                                                                                                                                                                                cATGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATACTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGCAATTAAAGCTTATTATCACAAAGGTTCTGCAACAGATATCg               >  2:76219/1‑247 (MQ=255)                                                                                                                                                                                                                                 attgTCATCACTCACACGGCAAGCCCTTAACAGAAGGCGAACCAAAAGCTTCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAgatg          <  2:544077/248‑1 (MQ=255)                                                                                                                                                                                                                                 aTGGTCATCACTCACACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAgatg          <  2:49147/251‑1 (MQ=255)                                                                                                                                                                                                                                         cactcacACGGCAAGCCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCAt  <  2:412150/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             CCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATTGT‑TAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAACTAAAACATTTGCTGAAATTAAAGATTATTATCACAAAGGTTATGCAACAGATATCGAGATGATTGGCAT  >  NC_000913/2041222‑2041703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑