Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	intergenic (+589/+167)	rtcR → / ← glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	98.9%	352.1 / ‑4.8	92	intergenic (+589/+167)	rtcR/glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (45/46);  total (45/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCG‑TGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGC  >  NC_000913/3560213‑3560696                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                  ttcccataaaggttttagcacggtggagaTTGTTGGTGACAAGGCGAAATTTG‑TTGTGATGGGGCGGGGCGTGCG‑TTCCGCCTCCGGGGGTGGTGCCGATATGGATAAACAGCGTGGAGCTATTGGGGTTTTG‑C‑TCC‑GCCCCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCCCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAt                                                                                                                                                                                                                                             <  2:326086/225‑1 (MQ=255) taaccaccacctggtTAGCAGCTTTGAAATTTTTGGGGACAAGCCGCAAATTG‑CTGTGATTGAGCAGGGCGTGCGCTACCGCTTCGGGGG‑TGGGGCGGATATCGATAACCGGCGTCGAGCCATTGGGGATTGG‑C‑TCC‑GCCACTTTGCGGGCGAGGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAt                                                                                                                                                                                                                                             <  2:135380/237‑1 (MQ=255)  tACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCG‑TGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAt                                                                                                                                                                                                                                             >  1:135380/1‑249 (MQ=255)     cATCACCGTGTTAGCAACGTTGAGTTTGTTGGTGACAATGCGAAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTCCCGCTCCCGGCG‑TGGTGCCGATATCGAAAAACAGCGTCGGCCCATTGGGGATTTC‑C‑CCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGt                                                                                                                                                                                                                                        <  1:640462/251‑1 (MQ=255)     cATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCG‑TGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGt                                                                                                                                                                                                                                        >  1:468617/1‑251 (MQ=255)     cATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCG‑TGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGt                                                                                                                                                                                                                                        >  1:518137/1‑251 (MQ=255)      cccAACGTTTTAGCAACTTTGAGATTGTTGGGGACAATGCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCG‑TGGTGCCGATACCGAAAACCAGCGTCGAGCCATTGGGGTTTTG‑C‑TCC‑GCCCCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGtt                                                                                                                                                                                                                                       <  1:145222/249‑1 (MQ=255)       tCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCG‑TGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCCGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGttt                                                                                                                                                                                                                                      >  2:220660/1‑251 (MQ=255)            gtgtTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCG‑TGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTc                                                                                                                                                                                                                                 >  1:39458/1‑251 (MQ=255)                       gTTGAGATTGTTGGTGACAATGCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCG‑TGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCAt                                                                                                                                                                                                                      >  1:567847/1‑251 (MQ=255)                               tgttgGTAACATTGCGCAA‑TTGTCTGTGGTTGAGCAGGGCGGCCGCTCCCGCTCCGGGCG‑TGTTGCCGATACCCATAAACAGCGTCGGGCAATTGGGGTTTTC‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGTTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGTACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTc                                                                                                                                                                                                               <  1:358968/250‑1 (MQ=255)                                ccggatctcaaggcgaaatttG‑CTGTGTTTGAGCAGTGGGGGCGCTCCCGCTTCCGGGG‑GGGTGCCGATATGGAAAAACGGCGTGGGGCCATGGGGGATTGG‑C‑CCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCGGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACGGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcg                                                                                                                                                                                                              <  1:492559/231‑1 (MQ=255)                                   ggTGACAATGCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCG‑TGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggc                                                                                                                                                                                                          >  2:354846/1‑251 (MQ=255)                                        ccaatgcgaaattgggtgtgTTTGGGCAGTGCGTGCGCTCCCGTTTCCGGCG‑TGGTGCCTATATCGATAAACAGCGTCGAGCCATGGGGGTTTTG‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCCCCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAt                                                                                                                                                                                                      <  1:523718/235‑1 (MQ=255)                                        cAATGCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTCCCGCTTCCGGCG‑TGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGCACTGGAAGGCAGCGCCGCCCCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcg                                                                                                                                                                                                              >  2:492559/1‑242 (MQ=255)                                         aaTGCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCG‑TGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAt                                                                                                                                                                                                      >  1:250458/1‑249 (MQ=255)                                                 aaTTG‑CTGTGTTTGGGCAGTGCGTGCGCTCCCGCTTCCGGCG‑TGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCggg                                                                                                                                                                                            <  1:451969/251‑1 (MQ=255)                                                 aaTTG‑CTGTGATTGAGCAGTGCGTGCGCTCCCGCTTCCGGCG‑TGGTGCCGATATCGAAAAACAGCGTCGAGCCATTGGGGATTTG‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCggg                                                                                                                                                                                            <  1:323042/251‑1 (MQ=255)                                                       gtgtgatggagcggggtgtgcccccccGCTTCGGGGG‑TGGTGCCGATAACAAAAACCGGCGGCGAGC‑AATGGGGGTTTGTC‑TCC‑GCCACTTGGCGGGCGATGCGCTTTTTTTCTTCGGTCTGGGGGGCCTTGCGATCGTGCCCCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGAc                                                                                                                                                                                       <  2:208294/226‑1 (MQ=255)                                                        tgtgATTGAGCAGTGCGTGCGCTACCGCTTCCGGCG‑TGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑C‑TCC‑GCCACTTTGCGGGCGATGCACTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACg                                                                                                                                                                                      >  1:330691/1‑251 (MQ=255)                                                           gATTGAGCAGTGCGTGCGCTACCGCTTCCGGCG‑TGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACgg                                                                                                                                                                                     >  1:391924/1‑249 (MQ=255)                                                            aTTGAGCAGTGCGTGCGCTACCGCTTCCGGCG‑TGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACgg                                                                                                                                                                                     >  1:131798/1‑248 (MQ=255)                                                                  cAGTGCGTGCGCTACCGCTTCCGGCG‑TGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGct                                                                                                                                                                            >  1:93943/1‑251 (MQ=255)                                                                    gtgcgtgcGCTCCCGCTTCCGGCG‑TGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGTATTTG‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGctct                                                                                                                                                                          <  1:13580/251‑1 (MQ=255)                                                                     tgcgtgcgCTACCGCTTCCGGCG‑TGGAGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGGGTTAACCGAACTGGCAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGCGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGctct                                                                                                                                                                          >  2:462635/1‑250 (MQ=255)                                                                      gcgtgcgCTACCGCTTCCGGGG‑TGGTGCCGATATCAATAAACAGCGTCGAGCCATTGGGGTTTTG‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAc                                                                                                                                                                        <  1:487210/251‑1 (MQ=255)                                                                            gcTACCGCTTCCGGCG‑TGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGctc                                                                                                                                                                  >  2:298572/1‑251 (MQ=255)                                                                             cTACCGCTTCCGGCG‑TGGTGCAGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGctct                                                                                                                                                                 >  2:542969/1‑251 (MQ=255)                                                                               aCCGCTTCCGGCG‑TGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTc                                                                                                                                                               >  1:506175/1‑251 (MQ=255)                                                                                           g‑tggtgCCGATACCGATAAACAGCGTCGGGCCATTGGGGATTTG‑C‑CCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATa                                                                                                                                                   <  1:59163/251‑1 (MQ=255)                                                                                               gtgCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAcc                                                                                                                                                >  2:423595/1‑251 (MQ=255)                                                                                                ggCCGATATAAATAAACAGCGCCGCCCCATGGGGTATTTG‑C‑TCC‑GCCTCTTTGCGGGCGATGCGCCCTTTTTCTTCGGTCTGGGTGGCTTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCGCCGCCATGATGG‑CGCAGGGTCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAccc                                                                                                                                               <  1:617165/250‑1 (MQ=255)                                                                                                 gCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                                                                                                                              >  2:429661/1‑251 (MQ=255)                                                                                                   cGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgc                                                                                                                                            >  1:435810/1‑251 (MQ=255)                                                                                                     atatCGATAAACAGCGTCGAGCCATTGGGGATTTG‑C‑TCC‑GCCACTTTGCGAACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGGACTGGATGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAGCCGGTTTTGCTACGCCAGCTCATTGAGGTCGCGGAGAATAATCTGCGTGCTGACGGCAAAATGCTCTACCAGCTCTTCGGTACTGCCATAACCctgctg                                                                                                                                          >  2:358562/1‑251 (MQ=255)                                                                                                          ataaaagcgccgccgacccttGGGGGTTTT‑C‑TCCCGCCACTTTGCGGGCGATGCGTTCTTTTTCTTCGGTCTGGGGGGCCTTGCGATCGTGCCCCGGCGTGTTAACCGAACTGGAAGGCCGCGCCGCTCCGCCATGATGG‑CGCAGGATCAGGTTGTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTa                                                                                                                                      <  2:567847/232‑1 (MQ=255)                                                                                                            tAAACAGCGTCGAGCCATTGGGGATTTG‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAcc                                                                                                                                   >  1:473477/1‑251 (MQ=255)                                                                                                                 aGCGTCGAGCCATTGGGGATTTG‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTc                                                                                                                              >  1:205845/1‑251 (MQ=255)                                                                                                                  gcgacgggCCATTGGGGATTGG‑C‑TCC‑GCCACTTGGCGGGCGTTGCGCTCTTTTTCTTCGGTTTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTATCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGTTTTTGCTCCGCCAGCTCATTGAGGTCTCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATACCCCTGCTGTTTAACCAGTTCg                                                                                                                             <  1:430452/244‑1 (MQ=255)                                                                                                                    gTCGAGCCATTGGGGATTTG‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGat                                                                                                                           >  1:239979/1‑251 (MQ=255)                                                                                                                      gtcggcgttggggggtttgtcccc‑GCCACGTGGCGGGCGAGGCGCTTTTTTCCTTCGGTCTGGGTGGCGTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGAGCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGat                                                                                                                           <  2:172474/229‑1 (MQ=255)                                                                                                                         ggccgtGGGGGTTTG‑CCTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCGGGGTGGCCTTGCGATCGTGCCACGGGGTGTTACCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataata                                                                                                                       <  2:160591/246‑1 (MQ=255)                                                                                                                            aTTGGGGATTTG‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGt                                                                                                                   >  1:211251/1‑251 (MQ=255)                                                                                                                               ggggATTTG‑C‑TCC‑GCCACTTTGCGGGCGCTGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCATGATCAGGTTTTGCTCCGCCCGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGTGAAATGCTCTACCAGCTCTTCGGTCATGACATAACCCTGCTGTTTAACCAGTTCGATAATATCGTtgt                                                                                                                >  2:128944/1‑251 (MQ=255)                                                                                                                               ggggATTTG‑C‑CCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtg                                                                                                                 <  1:429661/250‑1 (MQ=255)                                                                                                                                gggATTTG‑C‑TCC‑GCCCCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGGGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGTATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtgtg                                                                                                               <  1:242223/251‑1 (MQ=255)                                                                                                                                  gttttG‑C‑CCC‑GCCCCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGAc                                                                                                             <  2:9303/249‑1 (MQ=255)                                                                                                                                    tttG‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGt                                                                                                           >  1:67580/1‑251 (MQ=255)                                                                                                                                         c‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtgtg                                                                                                       >  1:317034/1‑251 (MQ=255)                                                                                                                                            cc‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCATCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtt                                                                                                     >  1:624269/1‑251 (MQ=255)                                                                                                                                             c‑GCCACTTTGCGGGCGATGCGCTTTTTTTCTTCGGTCTGGGTGGCCTTGCGCTCGGGCCACGGCGTGTTACCCGAACGGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgttt                                                                                                    <  2:518137/251‑1 (MQ=255)                                                                                                                                               gCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttg                                                                                                   <  1:446999/251‑1 (MQ=255)                                                                                                                                                ccACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttgt                                                                                                  >  2:343684/1‑251 (MQ=255)                                                                                                                                                 cACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttgtt                                                                                                 >  1:143490/1‑251 (MQ=255)                                                                                                                                                 atcTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGTGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCAGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTCCGCTAAACCCGTTGTGACGTTGtgtttgtt                                                                                                 >  2:69167/3‑251 (MQ=255)                                                                                                                                                     ttccGGGCGTTGCGCTCTTTTTCTTCGGTCGGGGGGGCCTGGCGAGCGTGCCACTGCGTGTTAACCGAACTGGAGGGCAGCGCCCCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTgtttgttt                                                                                                <  2:407050/245‑1 (MQ=255)                                                                                                                                                      ggtgggCGAGGCGGTCTTGTTCGTCGGTCTGGGTGGCCCTGCGTTCGTGCCACGGGTTGTTAACGGATCTGGAAGGCCGCGCGGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCTGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCAtt                                                                                            <  2:96352/248‑1 (MQ=255)                                                                                                                                                       gggggCGTTGCGCTTTTTTTCGTCGGTCTGGGTGGCCTTGCGACCGTGCCACGGGGTGTAAACCGAACGGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCAtt                                                                                            <  2:64212/248‑1 (MQ=255)                                                                                                                                                       gCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCAttt                                                                                           >  2:110912/1‑251 (MQ=255)                                                                                                                                                          ggCGAGGCCCTCTTTTTTGTCGGTCTGGGTGGCCTTGGGATCGTGCCAGGGCGTGTAAACCGAACTGGAAGGCAGCGCCGCCCCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTtata                                                                                        <  2:617906/251‑1 (MQ=255)                                                                                                                                                               tgcgtgctTTTTCTTCGGTCGGGGGGCCCTTGCGACCGGGCCCCGGTGGGTTAACCGAACTGGAAGGCTGCGCCGCCCCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATc                                                                                    <  2:250458/244‑1 (MQ=255)                                                                                                                                                                gcgcTTTTTTTCTTCGGTTGGGGTGGCCTTGCGATCGGTCCACGGCGTTTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATccc                                                                                  <  2:377754/251‑1 (MQ=255)                                                                                                                                                                            ttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAtttt                                                                      >  1:230420/1‑251 (MQ=255)                                                                                                                                                                               ggTCTTGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgt                                                                   >  1:243551/1‑251 (MQ=255)                                                                                                                                                                                gtcggggTGGCCTTGCGATCGGCCCACGGCGTGTAACCCAAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgtt                                                                  <  2:86846/247‑1 (MQ=255)                                                                                                                                                                                  cggggtttccTTGCGATCGTGCCCCGCCGTTTTAACCGAATTGGAAGTCAGCTCCGCCCCGCCATGATGT‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATTCTCTTCCATCTCTTCGTTACTGACATATCTCTGCTTTTTAACCAGTTCGATAATACCGTTGTGACGTTTTGTTTGTTTCATTTATAAATCCCTGTAAGTAttttcgtttt                                                                <  2:518927/243‑1 (MQ=255)                                                                                                                                                                                    ggggggCCTTGCGATCGGGCCACGGCGGGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgttttcg                                                              <  2:468617/247‑1 (MQ=255)                                                                                                                                                                                    gggTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgttttcg                                                              >  1:535208/1‑251 (MQ=255)                                                                                                                                                                                      ggggCCGTGCGTTCGGGCCACGGCGTGTTAACCGACCTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTcgcg                                                            <  2:293186/249‑1 (MQ=255)                                                                                                                                                                                      gTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTcgcg                                                            >  1:372561/1‑251 (MQ=255)                                                                                                                                                                                          ccTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCAtt                                                        >  1:305119/1‑251 (MQ=255)                                                                                                                                                                                              gCGATGGTGCCACGGCGTGTTACCCGACCGGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGc                                                    <  2:399778/251‑1 (MQ=255)                                                                                                                                                                                              gCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGc                                                    <  1:291279/251‑1 (MQ=255)                                                                                                                                                                                              gCGATCGGGCCACGGCGTTTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAg                                                     <  1:462635/250‑1 (MQ=255)                                                                                                                                                                                               agATCGTGCCACGGCGTGTTAACAGAAATGGCAGGCAGCGCCGCCCCGCCATGATGG‑CGCAGGACCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGCCGGAGACATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTACCCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATACATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCg                                                   >  2:556857/2‑251 (MQ=255)                                                                                                                                                                                                     ggCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaa                                             <  2:211251/250‑1 (MQ=255)                                                                                                                                                                                                          gggggggtaagcagatcggtagggCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTGTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACaaaa                                        <  2:391924/229‑1 (MQ=255)                                                                                                                                                                                                          cggggtgtTACCCGAACGGGAAGGCAGCGCCGCCCCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACaaaa                                        <  2:239979/247‑1 (MQ=255)                                                                                                                                                                                                             cGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc                                     >  2:121784/1‑251 (MQ=255)                                                                                                                                                                                                               tgtTAACCGAACTGGAAGTCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc                                     <  1:573045/249‑1 (MQ=255)                                                                                                                                                                                                                gtTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATc                                  >  2:365757/1‑251 (MQ=255)                                                                                                                                                                                                                  tAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCg                                 <  2:131798/250‑1 (MQ=255)                                                                                                                                                                                                                           gggaaggaagggccgacccGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTGGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAAAAAAAGCCAGCGCTAAACCCAc                       <  2:473477/234‑1 (MQ=255)                                                                                                                                                                                                                                ggccgcgcCGCACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCTTTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTa                  <  2:317034/247‑1 (MQ=255)                                                                                                                                                                                                                                       cctaaCCGCCATGATGGGA‑GAGGATCAGGTTTTGCACCGCCAGCCCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGGAATGCTCTACCAGCTATTCGGTACTGACATATCCGTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCAGTTATAAATCCCTGGGATTATTATCGTTGTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGc           <  2:231770/247‑1 (MQ=255)                                                                                                                                                                                                                                         gcACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCg          >  2:429728/1‑250 (MQ=255)                                                                                                                                                                                                                                         gcACCGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCg          >  1:241571/1‑250 (MQ=255)                                                                                                                                                                                                                                            ccGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATAAACAAAACCCATCGCTAAACCCACGGCTAACCCGGCgtggg      >  1:39396/1‑247 (MQ=255)                                                                                                                                                                                                                                             cGCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGAtgtg     >  1:405610/1‑251 (MQ=255)                                                                                                                                                                                                                                              gCCATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGAtgtgt    <  1:110912/251‑1 (MQ=255)                                                                                                                                                                                                                                               ccATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGGGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGAtgtgtg   <  2:408556/251‑1 (MQ=255)                                                                                                                                                                                                                                                cATGATGG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGc  <  1:499842/251‑1 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                  TTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCG‑TGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑C‑TCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGC  >  NC_000913/3560213‑3560696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑