Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,790,153	C→T	P58S (CCC→TCC)	lhgD →	L‑2‑hydroxyglutarate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,790,153	0	C	T	100.0%	217.9 / NA	65	P58S (CCC→TCC)	lhgD	L‑2‑hydroxyglutarate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/30);  total (35/30)

TGCGCCAGCGTGGCTATTTCGCTTACGCCTCTAACCACTACCAGACGCATCAGTAAGCGCAAAGGAATTGAGCGGATGT‑ATGATTTTGTGATTATTGGCGGCG‑GCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTC  >  NC_000913/2789907‑2790403                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                           aggcccaacgaaaagctctattgtttacgttgggcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGT‑ATGATTTTGTGATTATTGGCGGCG‑GCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCgg                                                                                                                                                                                                                                                        >  1:448224‑M2/57‑251 (MQ=255)       aacgaaaagctctattgtttacgttgggcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGT‑ATGATTTTGTGATTATTGGCGGCG‑GCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCt                                                                                                                                                                                                                                                  >  1:392159‑M2/51‑251 (MQ=255)         cgaaaagctctattgtttacgttgggcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGT‑ATGATTTGGTGATTATTGGCGGCG‑GCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCCCCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGa                                                                                                                                                                                                                                                <  1:498332‑M2/203‑1 (MQ=255)         cgaaaagctctattgtttacgttgggcctaaacgcagggagactccccGCGCAAAGGAATGGAGCGGATGT‑ATGATTTTGTGATTATTGGCGGCG‑GCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGa                                                                                                                                                                                                                                                <  1:486182‑M2/203‑1 (MQ=255)                 tctattgtttacgttgggcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGT‑ATGATTTTGTGATTATTGGCGGCG‑GCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCACGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGgcgc                                                                                                                                                                                                                                          >  1:82230‑M2/41‑249 (MQ=255)                     ttgtttacgttgggcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGT‑ATGATTTTGTGATTATTGGCGGCG‑GCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACCGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGCAGGCGCAGgttt                                                                                                                                                                                                                                    >  2:392334‑M2/37‑251 (MQ=255)                      tgtttacgttgggcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGT‑ATGATTTTGTGATTATTGGCGGCG‑GCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGtttt                                                                                                                                                                                                                                    <  1:451868‑M2/215‑1 (MQ=255)                           acgttgggcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGT‑ATGATTTTGTGATTATTGGCGGCG‑GCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTg                                                                                                                                                                                                                               <  1:422694‑M2/220‑1 (MQ=255)                                gggcaaaaacgaggggagcaccccggcgcAAGGGAATTGGGCGGATGT‑AGGATTTTGTGATTTTGGGCGGCG‑GCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCAGGGCGTGGCGGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGGCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGa                                                                                                                                                                                                                         <  2:234752/226‑1 (MQ=255)                                   cctaaacgcagggagaccccccTCGCAAAGGAATTGAGCGGATGT‑ATGATTTTGTGATTATTGGCGGCG‑GCATCATCGGCATGTCGCCCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAAGGTCCGCCCCGGCCGGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAAc                                                                                                                                                                                                                      <  1:446148‑M2/229‑1 (MQ=255)                                      aaacgcagggggacaccccGCGCAAAGGAATTGAGCGGA‑GTGAGTATTTTGTGATTATTGGCGGCG‑GCATCATCGGCATGTCGTCCGCCATGCAACGGATGGTTGTCTTTCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCGGTCCCCAGACGGGCCGCAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACcgc                                                                                                                                                                                                                   <  2:121354‑M2/232‑1 (MQ=255)                                      aaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGT‑ATGATTTTGTGATTATTGGCGGCG‑GCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAAcc                                                                                                                                                                                                                     <  1:121785‑M2/230‑1 (MQ=255)                                          gcagggagactccccGCGCAAAGGAATTGAGCGGATGT‑ATGATTTTGTGATTATTGGCGGCG‑GCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAAAAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCgccc                                                                                                                                                                                                               >  1:192440‑M2/16‑250 (MQ=255)                                                agcctccccGCGCAAAGGAAATGAGCGGCTGT‑ATGATTTTGTGATTATTGGCGGCG‑GCATCATTGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCCGGAAAAAGACTCCGCCCCCGCCTGTCACCAGACGGGCCACAACACCGGCGTGATCCATGCCGGGGCCTATTACCCGTGCGGCCGCCTGAAGGCGCAGTTTGGTCTGGCGGGAAACCGCGCCACCaa                                                                                                                                                                                                           >  2:370910‑M2/10‑249 (MQ=255)                                                        caggaaaaGTAATTGGGCGGATGT‑AGGATTTGGTGTTTATGGGCGGCG‑GCATCAGCGGCAGGCCGACCGCCATGCAACTGATTGATGTCTACCCGGACGCCCGCATTTCTTTGCTGGAAAAAGAGCCCTCCCCGGCCTGTCCCCAGACGGGCCACAACAGCGGCGTGATCCAGGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGc                                                                                                                                                                                                 <  2:262681/246‑1 (MQ=255)                                                         gcgcAAAGGAATTGAGCGGATGT‑ATGATTTTGTGATTATTGGCGGCG‑GCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCg                                                                                                                                                                                                >  1:2817/1‑251 (MQ=255)                                                           gcAAAGGAATTGAGCGGATGT‑ATGATTTTGTGATTATTGGCGGCG‑GCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGAt                                                                                                                                                                                              >  1:517887/1‑251 (MQ=255)                                                           gcAAAGGAATGGAGCGGATGT‑ATGATTTTGTGATTATTGGCGGCG‑GCATCATCGGCATGTCGCCCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGATTCCGCCCCGGCCTGTCCCCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACAAAAGCCTTTTGCGAt                                                                                                                                                                                              <  1:162878/251‑1 (MQ=255)                                                                         cGGATGT‑ATGATTTTGTGATTATTGGCGGCG‑GCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCg                                                                                                                                                                                >  1:411468/1‑251 (MQ=255)                                                                              gT‑ATGATTTTGTGATTTTTGGGGGGG‑GCATCACGGGCAGGCTGCCCGCCATGCAACGGTTTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACg                                                                                                                                                                           <  2:102975/251‑1 (MQ=255)                                                                              gT‑ATGATTTTGTGATTATTGGCGGCG‑GCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACg                                                                                                                                                                           >  1:280042/1‑251 (MQ=255)                                                                                       ttGTGATTATTGGCGGCG‑GCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGc                                                                                                                                                                   >  1:359274/1‑251 (MQ=255)                                                                                       ttGTGATTATTGGCGGCG‑GCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGc                                                                                                                                                                   >  1:401589/1‑251 (MQ=255)                                                                                        tgtgATTATTGGCGGCG‑GCATCATCGGCATGTCGCCCGCCATGCAACTGATTGATGCCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGGGATCCATGCCGGGGTGTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTTGCTGGCGGGAAACCGCGACACCCAAGCGTTTTGCGCACAAAAAGGCATACGCTGCGACaaacgcg                                                                                                                                                                  >  2:367991/1‑246 (MQ=255)                                                                                         gtgATTATTGGCGGCG‑GCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGCCGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCg                                                                                                                                                                  <  1:425752/250‑1 (MQ=255)                                                                                           gATTATTGGCGGCG‑GCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAACAAGAGTCCGCCCCGGCCTGTCACCAGACAGGCCACAACAGCGGCGTGATCCATGCCGGGGCCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGCTCAAAACGGCAATCGCTACGACAACTGCGGcc                                                                                                                                                               >  1:260445/1‑250 (MQ=255)                                                                                           gATTATTGGCG‑CGTGCATCACCGGCGTGTCGCCCGCCATGCACCGAATGGATGTCTCCCGGGACGCCCGCATTGCGTTCCGGAAAAAAGAGTCCGCCCCGGCCTGTCCCCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCggg                                                                                                                                                                <  2:61954/250‑2 (MQ=255)                                                                                            attattGGCGGCG‑GCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGCAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCCGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCCCCAAAGCCTTTTGCGATCAAAACGGCATTCGCCACGACAACTGCGGCa                                                                                                                                                               >  2:13417/1‑250 (MQ=255)                                                                                                      gcg‑gcATCACGGGCATGTCGACCGCCATGCAACTGATGGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGtt                                                                                                                                                    <  2:359274/251‑1 (MQ=255)                                                                                                                  ggCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCg                                                                                                                                          >  1:393738/1‑250 (MQ=255)                                                                                                                  ggCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCg                                                                                                                                          >  1:137931/1‑250 (MQ=255)                                                                                                                     aggtccaCCGCAATGCACCTGATTGTTGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGCCGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGAtct                                                                                                                                      <  2:501339/245‑1 (MQ=255)                                                                                                                          gACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAATAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGaa                                                                                                                                  >  1:509114/1‑250 (MQ=255)                                                                                                                                          attgatGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATgcgcgc                                                                                                                 >  1:390386/1‑251 (MQ=255)                                                                                                                                              atGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCtt                                                                                                              >  1:194034/1‑250 (MQ=255)                                                                                                                                                   gtcccggcggcccgctgggagtggCTGGAAAAGGGGTCGGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTTTTCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTGTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGa                                                                                                        <  2:515662/228‑1 (MQ=255)                                                                                                                                                          aCGCCCGCATTGCGTTGCGGAAAAAAGAGTCCGCCCAGGCCTGTCCCCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAATGcaca                                                                                                 <  1:231842/251‑1 (MQ=255)                                                                                                                                                              ccGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAgtgg                                                                                             >  2:508223/1‑248 (MQ=255)                                                                                                                                                                     gctttGCTGGAAAAAGGGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACgg                                                                                      <  2:126040/248‑1 (MQ=255)                                                                                                                                                                      ggttgcgtgAAAAAGAGTCCGCCCCGGCCTGTCCCCAGGCGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGt                                                                                     <  2:489344/243‑1 (MQ=255)                                                                                                                                                                       gTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGt                                                                                     >  1:314043/1‑250 (MQ=255)                                                                                                                                                                              gAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAgcg                                                                             >  1:342698/1‑251 (MQ=255)                                                                                                                                                                                 caaGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGcgcg                                                                           >  2:204266/2‑250 (MQ=255)                                                                                                                                                                                   agcgTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGGATCGAGCGCGAAt                                                                        >  2:321146/4‑251 (MQ=255)                                                                                                                                                                                   agagTCCCCCCCGGCCTGTCCCCAGACGGGCGACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAAt                                                                        <  2:392159/251‑1 (MQ=255)                                                                                                                                                                                        ccgcccgtgCCTGTCACCGGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACCCGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCtt                                                                   <  2:236107/243‑1 (MQ=255)                                                                                                                                                                                         ccccccGGCGTGTCATCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTGTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGAGGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTa                                                                  <  2:82230/249‑1 (MQ=255)                                                                                                                                                                                          gCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGGCTATTACACGTCCGACAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTACGATCTCGAAATGGACCGGATGCGCGCCTTCTGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGCCTTa                                                                  >  2:516997/1‑250 (MQ=255)                                                                                                                                                                                                 ccTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCcgac                                                          <  1:254099/251‑1 (MQ=255)                                                                                                                                                                                                 ccTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCcgac                                                          >  2:309929/1‑251 (MQ=255)                                                                                                                                                                                                   tGTCTCCAGACGGGCTACACCAGCGTCGTGATCCAGGCCGGGGTCTATTACACGTCCTGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTGTGCGATCAAAACGGCATTCGCTACGACAACTGCGGTAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGAGGCGCGCCTTATGGGAACGCACAGTGGCGAACGGCATCGAGCGCGAATGGCTTAACGCcgac                                                          <  2:221003/249‑1 (MQ=255)                                                                                                                                                                                                     tCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAAc                                                      >  1:75627/1‑251 (MQ=255)                                                                                                                                                                                                              gggCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGACCGCACCGTGGCGAACGGTATCGAGCGCGAAAGGCTTAACGCCGACGAACTgcgcgagc                                             >  2:81464/1‑251 (MQ=255)                                                                                                                                                                                                                  cacaACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTgcgcgagcgcg                                          >  1:145400/1‑250 (MQ=255)                                                                                                                                                                                                                   acaacaGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGaa                                        <  1:384256/251‑1 (MQ=255)                                                                                                                                                                                                                    caacaGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAAc                                       <  2:132255/251‑1 (MQ=255)                                                                                                                                                                                                                    caacaGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAAc                                       <  2:482599/251‑1 (MQ=255)                                                                                                                                                                                                                           ggcgTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAatat                                >  1:37602/1‑251 (MQ=255)                                                                                                                                                                                                                             cgTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGCACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATc                               >  2:98694/1‑250 (MQ=255)                                                                                                                                                                                                                                gATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCAcc                            <  2:2817/250‑1 (MQ=255)                                                                                                                                                                                                                                gATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCAcc                            <  1:13417/250‑1 (MQ=255)                                                                                                                                                                                                                                 aTCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCgg                          <  1:198445/251‑1 (MQ=255)                                                                                                                                                                                                                                              tCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCAtt             >  1:284796/1‑251 (MQ=255)                                                                                                                                                                                                                                              tCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCAtt             >  1:178249/1‑251 (MQ=255)                                                                                                                                                                                                                                                     cacGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACAGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGc      >  1:523940/1‑251 (MQ=255)                                                                                                                                                                                                                                                         cccGGCAGCCTGAAGGCGCAGGTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTc  <  2:300817/251‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                           TGCGCCAGCGTGGCTATTTCGCTTACGCCTCTAACCACTACCAGACGCATCAGTAAGCGCAAAGGAATTGAGCGGATGT‑ATGATTTTGTGATTATTGGCGGCG‑GCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCGTC  >  NC_000913/2789907‑2790403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑