Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,628,332	T→C	intergenic (+69/‑20)	ydfG → / → rspR	3‑hydroxy acid dehydrogenase YdfG/DNA‑binding transcriptional repressor RspR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,628,332	0	T	C	100.0%	118.9 / NA	34	intergenic (+69/‑20)	ydfG/rspR	3‑hydroxy acid dehydrogenase YdfG/DNA‑binding transcriptional repressor RspR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/23);  total (11/23)

TAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGCAAGAACGAATGACCGTCGAAACGCAACTTAATCCCACACAGCCTGTCAATCAGCAGATTTATCGTATTCTTCGTCGCGACATTGTCCATTGCCTGATTGCTCCAGGCACACCGTTGTCGGAAAAAGAAGTTTCTGTTCGTTTCAATGTGTCACGCCAGCCGGTTCGTGAAGCCTTTATTAAACTGGCGGAAAACGGCCTGATTCAAATTCGTCCGCAACGT  >  NC_000913/1628086‑1628564                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                         tAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAATATGCCGTGGCGTTGACGCCAGAAGATGTCAGCGAAGTCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGGCAATATCAATACCCTGGAAATGATTCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGGAATTTTTATACCCGGCGTAACTTCTGGGTTATTGCTTGTCACAAAAAAGTGGTAGACGCATTCGGTTAacccac                                                                                                                                                                                                                                       <  2:366133/250‑1 (MQ=255)   aaGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAacccactc                                                                                                                                                                                                                                     >  2:469464/1‑250 (MQ=255)    aGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACccactcac                                                                                                                                                                                                                                   >  2:156044/1‑251 (MQ=255)     ggCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACAGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACt                                                                                                                                                                                                                                  >  2:326355‑M1/1‑250 (MQ=255)          tGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatg                                                                                                                                                                                                                             >  1:300923‑M1/1‑245 (MQ=255)                        aaaaaaCCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggac                                                                                                                                                                                                               <  2:300923‑M1/251‑21 (MQ=255)                             aCCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggaacttt                                                                                                                                                                                                           >  2:326094‑M1/1‑226 (MQ=255)                                 agcaaagtagcggtgcattgacgccagAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATGCCCTGGAAATGATGCAGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAGAAGGGGTAGACTCATGCAGTTAACCCACTCACtgcatgaacagcggcaggaccttgaggt                                                                                                                                                                                                       <  2:212850‑M1/237‑29 (MQ=255)                                         aCCGGTGCATTGACGCAAGACGATGTCGGCGAAGCCGTCTGGTGGGGGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcagggcctttaggtccgttctc                                                                                                                                                                                               >  2:22695‑M1/1‑214 (MQ=255)                                              tGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaatacc                                                                                                                                                                                         >  2:287227‑M1/1‑209 (MQ=255)                                                 aTTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGTTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGGCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaa                                                                                                                                                                                      <  1:324263‑M1/251‑46 (MQ=255)                                                    gACGCCAGAAGATGTCAGCGAAGCCGTCTGGTTGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACttcatgaacagcggcaggacctttaggtccgttctcaataccaaattc                                                                                                                                                                                   <  1:12318‑M1/251‑49 (MQ=255)                                                       gCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaac                                                                                                                                                                                <  1:491432‑M1/251‑52 (MQ=255)                                                          agaagaTGTCAGCGAAGCCGGCTGGTTGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGTAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGGCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATTCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttc                                                                                                                                                                             <  2:384826‑M1/251‑55 (MQ=255)                                                              gaTGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaactgcctga                                                                                                                                                                         <  2:210925‑M1/251‑59 (MQ=255)                                                                                 ggtgggtgTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggaccgttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttg                                                                                                                                                       <  2:202476‑M1/250‑77 (MQ=255)                                                                                 ggggggtgtcaACGCTGGCTGATGACGGCAATATCAATACCCTTGAAATGATGCCGGTTACCAAAAGCTATGCAGGACTGAATGTCCAACGTCCGTAAATTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttga                                                                                                                                                      <  2:352688‑M1/248‑78 (MQ=255)                                                                                             cGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctct                                                                                                                                          <  1:309767‑M1/251‑90 (MQ=255)                                                                                                 gcctgcTCACGTCAATATCAATTCCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatctttgctctgaat                                                                                                                                      <  2:441836‑M1/251‑94 (MQ=255)                                                                                                 gcctgcTCACGTCAATATCAATTCCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaat                                                                                                                                      <  2:378744‑M1/251‑94 (MQ=255)                                                                                                  cctgcTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaatt                                                                                                                                     <  1:156044‑M1/251‑95 (MQ=255)                                                                                                     gcTCAAGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATCTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTTCTTGTCGCAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACttcatgaacagcggcaggacctttagtgccgttctcaataccaaatgcagctgccatattcccagttaatgttttgaaatcgttgctctgaattg                                                                                                                                    <  1:409221‑M1/249‑96 (MQ=255)                                                                                                                  tcaataCCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtacgttctcaataccaaattcaacttcctgattcgcagttaatgttttgaaatcgttgctctgaattgctgagaaatagaca                                                                                                                      >  2:30093‑M1/1‑141 (MQ=255)                                                                                                                    aataCCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaat                                                                                                                                                             <  1:411802‑M1/209‑71 (MQ=255)                                                                                                                    aataCCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaat                                                                                                                                                             >  2:411802‑M1/1‑139 (MQ=255)                                                                                                                            ggAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgcctagaaatagacaaacacatct                                                                                                             <  2:188392‑M1/249‑119 (MQ=255)                                                                                                                                                      aTGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttccttattctcagttaatgtttttaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaa                                                                                   <  1:113625‑M1/249‑145 (MQ=255)                                                                                                                                                                gAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctt                                                                        <  1:326094‑M1/250‑156 (MQ=255)                                                                                                                                                                  aTGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaaccttttt                                                                     <  2:98945‑M1/251‑159 (MQ=255)                                                                                                                                                                                          aCCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattcccagttaatgttttgaaatcgttgctctgaattgctgcgaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttttcagggttaaaccatttcactaaac                                             >  2:264607‑M1/1‑69 (MQ=255)                                                                                                                                                                                              ggCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttttcagggttaaaccatttcactaaaccagt                                         >  1:416310‑M1/1‑65 (MQ=255)                                                                                                                                                                                                cGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttttcagggttaaaccatttcactaaaccagtca                                       <  1:319493‑M1/251‑189 (MQ=255)                                                                                                                                                                                                 gTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttttcagggttaaaccatttcactaaaccagtcat                                      >  2:305405‑M1/1‑62 (MQ=255)                                                                                                                                                                                                              tATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttttcagggttaaaccatttcactaaaccagtcattttgttagacat                          <  1:22695‑M1/250‑202 (MQ=255)                                                                                                                                                                                                                 tGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttttcagggttaaaccatttcactaaaccaggcattttgttagacataat                       <  2:94646‑M1/250‑205 (MQ=255)                                                                                                                                                                                                                               aaGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttttcagggttaaaccatttcactaaaccagtcattttgttagacataattattaccttttga          >  2:212816‑M1/1‑32 (MQ=255)                                                                                                                                                                                                                                      aGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttttcagggttaaaccatttcactaaaccagtcattttgttagacataattattaccttttgaagaaatta  <  1:30093‑M1/250‑226 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                         TAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGCAAGAACGAATGACCGTCGAAACGCAACTTAATCCCACACAGCCTGTCAATCAGCAGATTTATCGTATTCTTCGTCGCGACATTGTCCATTGCCTGATTGCTCCAGGCACACCGTTGTCGGAAAAAGAAGTTTCTGTTCGTTTCAATGTGTCACGCCAGCCGGTTCGTGAAGCCTTTATTAAACTGGCGGAAAACGGCCTGATTCAAATTCGTCCGCAACGT  >  NC_000913/1628086‑1628564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑