Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,301,917	C→T	Q246Y (CAG→TAT)	oppA →	oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,301,917	0	C	T	100.0%	204.5 / NA	56	Q246Y (CAG→TAT)	oppA	oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/33);  total (23/33)

GATCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATA  >  NC_000913/1301680‑1302160                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     gatCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAttt                                                                                                                                                                                                                                         <  2:168173/251‑1 (MQ=255)    cacacaTTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑cc                                                                                                                                                                                                                                     >  1:238112/1‑251 (MQ=255)        caTTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTAtt                                                                                                                                                                                                                                 <  2:36582/251‑1 (MQ=255)                gtcccctttttttcccccGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTg                                                                                                                                                                                                                          >  1:276605/16‑250 (MQ=255)                  cACCTTCAGTGAACCCGTTCCGCACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAACAGCCGCTATCGAGAAATTCGGCTAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAAAGAACGAATCGTGCTTGAACGAATCCCGACCTCCTGGAACAACACGAAAACCGTTATTAACTATGTAACCTATTTG‑CATATTCCTTCTGa                                                                                                                                                                                                                         >  2:20002/1‑249 (MQ=255)                        aaGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTAcc                                                                                                                                                                                                                  <  2:518736/250‑1 (MQ=255)                              aCCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCGGATATCGAGAAATTCGGCGAAAAATGGAGCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAGCAACGCGAAAACCGTTATTAACTATGTAACCTATATG‑CCGATTGCTTCTGAAGTTACCGATGTCa                                                                                                                                                                                                           <  1:481054/251‑1 (MQ=255)                                  gTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCg                                                                                                                                                                                                       >  1:18124/1‑251 (MQ=255)                                       gTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTAcc                                                                                                                                                                                                  >  1:503011/1‑251 (MQ=255)                                         aCTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCg                                                                                                                                                                                                 <  1:491202/250‑1 (MQ=255)                                                     tACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATc                                                                                                                                                                                    >  2:344351/1‑251 (MQ=255)                                                      aCTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATc                                                                                                                                                                                    <  1:374071/250‑1 (MQ=255)                                                               cccATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGa                                                                                                                                                                          <  2:14952/251‑1 (MQ=255)                                                                 cATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAAt                                                                                                                                                                        <  1:416196/251‑1 (MQ=255)                                                                         tCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTCAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAAcatcat                                                                                                                                                                >  2:272185/1‑251 (MQ=255)                                                                          cACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCAt                                                                                                                                                                <  2:362294/250‑1 (MQ=255)                                                                                    aaaaGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAAt                                                                                                                                                     >  1:473356/1‑251 (MQ=255)                                                                                        gCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGtt                                                                                                                                                 <  1:506276/251‑1 (MQ=255)                                                                                              aTCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGa                                                                                                                                            >  1:27138/1‑250 (MQ=255)                                                                                                cGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGc                                                                                                                                         <  1:356469/251‑1 (MQ=255)                                                                                                        tCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGaaaaa                                                                                                                                  <  2:62480/250‑1 (MQ=255)                                                                                                           gCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAagag                                                                                                                              <  1:510806/251‑1 (MQ=255)                                                                                                           gCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAag                                                                                                                                >  1:19992/1‑249 (MQ=255)                                                                                                                          aGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGtt                                                                                                               <  1:344351/251‑1 (MQ=255)                                                                                                                                gTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGtt                                                                                                         <  1:379689/251‑1 (MQ=255)                                                                                                                                gTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGtt                                                                                                         <  1:310120/251‑1 (MQ=255)                                                                                                                                       cGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGCTGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCAt                                                                                                  >  2:215643/1‑251 (MQ=255)                                                                                                                                          cactaaCGGTGCCTATCCCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGATATCCCGTACGAAGTTCACGTTGATCCATAcc                                                                                               <  1:379748/247‑1 (MQ=255)                                                                                                                                          cACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATAc                                                                                                <  2:125175/250‑1 (MQ=255)                                                                                                                                           aCCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCt                                                                                              >  1:285551/1‑251 (MQ=255)                                                                                                                                                 ggTGCCTATACCTTAAAAGATTGGGTCGGAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCAGGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCAc                                                                                        <  2:276605/251‑1 (MQ=255)                                                                                                                                                  gTGCCTATACCTTAAAAGATTGGGTCGCAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTATTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGATTTGTTCCAGAAGCTGGAAAAAGAGATCCCGGACGACGTTCACGTTGATCCATACCTGTGAACt                                                                                       >  2:294759/1‑251 (MQ=255)                                                                                                                                                               caaaGATCGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACATACTAGAACACCGAGAAAACCGTTGTTAACTATGTAGCATATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGACCGAATTGTTCCAGATGCTGAAAAAAGAGATGCCGGACGAAGTTCACGTTGATCCATACCTTTGCATTTACTATTACgca                                                                           >  2:426766/2‑248 (MQ=255)                                                                                                                                                                 aaGATGGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATGTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCTATCGAATTGTTCCAGAAGCTGAAAAAAGAGAACCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAAtt                                                                        <  2:105991/251‑1 (MQ=255)                                                                                                                                                                   gATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTa                                                                       >  1:262468/1‑250 (MQ=255)                                                                                                                                                                               aacgaacgAATCGTTCGTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCCCTTACTATTACGAAATTAACAACCAGAAAcc                                                          <  1:478441/251‑1 (MQ=255)                                                                                                                                                                                  gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGcc                                                       >  1:257491/1‑251 (MQ=255)                                                                                                                                                                                  gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGcc                                                       <  2:275515/251‑1 (MQ=255)                                                                                                                                                                                   aacgaaTCGTTCTTGAACGCAGCCCGACCTACGGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCa                                                      <  2:238112/251‑1 (MQ=255)                                                                                                                                                                                          cGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAAAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAAc                                                <  2:415763/250‑1 (MQ=255)                                                                                                                                                                                            ttcttgaatgacgCCCAACCTTCTGAAACAACGCGAAAACCTTTATTAACTATGTAACCTATTTC‑CGTATTTCGTCTTAAGTAACCGATGTCAACCGCTACCGTAGTGGGAAAATCGACATGACGAAGACCAGCGTGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGTACAAATTTCACGTTGAACCATACCTGTGCACTTGCTATTACAAAATTAACAACCAGAAACCGCCATTCAACGa                                              <  2:502311/238‑1 (MQ=255)                                                                                                                                                                                                tGAACGCAGCCAGACCTACTCGAACAACGCGAAAACCGTAATCAACTATGTAACCTAGTTG‑CCTTTTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGTTGAAATCGACAGGACGAATAACAGCATGCCGAGCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACAAAGTTCACGTTGATCCATACTTTTGCCCTTACTATTACCAAATGAACAACCAGACACCTCCATTCAACGAtgtg                                          <  2:187611/250‑1 (MQ=255)                                                                                                                                                                                                   aCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTCTTGCTTATGAAGATACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGCtgtgcgtg                                      >  1:421243/1‑251 (MQ=255)                                                                                                                                                                                                                       acaacGCGAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACt                   >  1:249601/1‑250 (MQ=255)                                                                                                                                                                                                                       acaacGCGAAAACCGTTATTAACTATGTAACCTATGTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTCCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCTTACCTGTGCACTTCCTATTACGAAATTAACACCCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTa                  <  2:18124/251‑1 (MQ=255)                                                                                                                                                                                                                         aacGCGAAACCCGTTATTAACTTGGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATTTCAACCGCTCCCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGCTCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGTCGAAGTTCCCGTTGACCCATCCCTGTGCACTTACTATTACGAAATTACCAACCAGACACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAgg                <  1:258236/251‑1 (MQ=255)                                                                                                                                                                                                                          ccgcgAAAACCGTTATTAAATATGTAAC‑TATTTGGCCTATAGCTTTTGAAGTTACGGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCAAGTTGATCCAGACCTTTGCCCTTCTTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGt               <  2:465439/250‑1 (MQ=255)                                                                                                                                                                                                                           cgcgAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGt               >  1:498350/1‑250 (MQ=255)                                                                                                                                                                                                                           cgcgAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTa              <  1:205887/251‑1 (MQ=255)                                                                                                                                                                                                                            gcgAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTc                                                                                                              >  2:229982/1‑154 (MQ=255)                                                                                                                                                                                                                            gcgAAAACCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTc                                                                                                              <  1:229982/154‑1 (MQ=255)                                                                                                                                                                                                                                aaaCCGTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGAc         >  1:54943/1‑251 (MQ=255)                                                                                                                                                                                                                                     gTTATTAACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACcgcg     >  2:288290/1‑250 (MQ=255)                                                                                                                                                                                                                                      ttattaACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTc                                                                                                                                                <  2:363606/110‑1 (MQ=255)                                                                                                                                                                                                                                      ttattaACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTc                                                                                                                                                >  1:363606/1‑110 (MQ=255)                                                                                                                                                                                                                                        attaACTATGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCAAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCAATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGata  <  2:75343/250‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     GATCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTG‑CCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATA  >  NC_000913/1301680‑1302160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑