BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,558,907	T→A	K207M (AAG→ATG)	iadA ←	isoaspartyl dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,558,907	0	T	A	96.8%	427.4 / NA	124	K207M (AAG→ATG)	iadA	isoaspartyl dipeptidase
Reads supporting (aligned to +/- strand): ref base T (0/4); new base A (64/56); total (64/60)
Fisher's exact test for biased strand distribution p-value = 5.20e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 124 total aligned reads displayed.

GTTGCCGTCGGAGCTGAGGGTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTT  >  NC_000913/4558666‑4559153

ggtGCCGTCGGAGCGGAGGGTGACGCTTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGt                                                                                                                                                                                                                                                 <  2:476333/249‑1 (MQ=255)

   gCCGTCGGAGCTGAGGGTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGc                                                                                                                                                                                                                                              >  1:352745/1‑251 (MQ=255)

     cGTCGGAGCTGAGGGTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTCGCTGATCGG‑CACATCGCAGGTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTATCGccc                                                                                                                                                                                                                                            >  2:557313/1‑251 (MQ=255)

         ggAGCTGAGGGTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgt                                                                                                                                                                                                                                        >  2:119247/1‑251 (MQ=255)

           ggCTGAGGGTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg                                                                                                                                                                                                                                      <  1:539526/250‑1 (MQ=255)

             cTGAGGGAGACGCGAGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGCGATATCGAAGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCCGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTggc                                                                                                                                                                                                                                     >  2:144256/1‑249 (MQ=255)

               gAGGGTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGaa                                                                                                                                                                                                                                    <  1:352255/249‑1 (MQ=255)

                      aCGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAAGGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACGCCAGCGCCTGCTCAAACAACG‑GTACGGTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCTCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTTCTGTCGCCCATGTGGAACACGG‑TGAc                                                                                                                                                                                                                          <  2:503476/251‑1 (MQ=255)

                       cgcgTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCTGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCATCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑g                                                                                                                                                                                                                        <  1:162826/251‑1 (MQ=255)

                          gTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑Gcc                                                                                                                                                                                                                      >  2:366025/1‑250 (MQ=255)

                           tGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGCCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGCCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑Gca                                                                                                                                                                                                                      >  1:444135/1‑248 (MQ=255)

                               aGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCg                                                                                                                                                                                                                                               >  1:566716/1‑222 (MQ=255)

                                  ggAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTAcc                                                                                                                                                                                                             >  2:414947/1‑251 (MQ=255)

                                    aaTACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTAccgc                                                                                                                                                                                                           <  2:43728/251‑1 (MQ=255)

                                     aTACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTAccgcc                                                                                                                                                                                                          <  1:430764/251‑1 (MQ=255)

                                     aTACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTAccgcc                                                                                                                                                                                                          <  1:520146/251‑1 (MQ=255)

                                      tACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTAccgccg                                                                                                                                                                                                         >  2:48128/1‑251 (MQ=255)

                                           gCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCaa                                                                                                                                                                                                    <  2:575764/251‑1 (MQ=255)

                                                aaCGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGc                                                                                                                                                                                               <  2:268044/251‑1 (MQ=255)

                                                 aCGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGcc                                                                                                                                                                                              >  2:318327/1‑251 (MQ=255)

                                                  gggCGCGGGCAATCCCGTCGGCAGGGGGTACCGGTTCGTCAATGCTGCTGGTGATTTCGATGGGGCCGCCGTTGCGCGCGAACTCCAGCGCCTGCGCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCGTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTCCTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGGGCAAACCGCCa                                                                                                                                                                                             <  2:435367/250‑1 (MQ=255)

                                                   ggCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCa                                                                                                                                                                                             >  1:132445/1‑250 (MQ=255)

                                                    gcgcGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCGGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCCCCCATGTGGAACACGG‑TGAC‑GCCAGGCTTACCGCCGAGCAAACCGCCAAc                                                                                                                                                                                           >  2:79514/1‑251 (MQ=255)

                                                       cgGGCAATCCCTTCGGCAGGGGAGACCGTTTCGCCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCCGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAAcgcg                                                                                                                                                                                        <  2:338096/251‑1 (MQ=255)

                                                       cgGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCCTGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAAcgcg                                                                                                                                                                                        >  2:482748/1‑251 (MQ=255)

                                                        gggCAATACCTTCGGCAGAGCCGACAGGTTCGTCAATGCTGCTGGTGTTATCCATGGTGCAGCCTTCGGGCGAGAACTCCAGCGGCGGCTCACACAACG‑GTACGTTGCGATTAAAGAG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGGAGGGCATAAATAGGCTGAAACGCCATTTTACTGTCGCCCATGTGGAAAACGG‑TGAC‑GCCAGGTGTACCGCCGAACAAACCGACATCGCgg                                                                                                                                                                                       >  2:423828/1‑251 (MQ=255)

                                                         ggCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCggg                                                                                                                                                                                      >  1:95853/1‑251 (MQ=255)

                                                            aatgccctCGGCGGGGGGGACCGGCTCGTCAATGCTGCTGGTGATAGCGATGTTGCCGCCTTTGCTCGCGACCGCCAGCGCCTGCTCAAACAACG‑GTGCGTTGCTGTTAACGTT‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACGGTCGCCCGTGTGGAACACGG‑TGCC‑GCCGGGTGTACCGCCGGGCAAACTGCCAACGCGGGAtt                                                                                                                                                                                   <  2:155898/244‑1 (MQ=255)

                                                            aaTACCTTCGGCAGGGGCGCCCGGTTCGGCAATGCTGCTGGTGATACCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGACCACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGAtt                                                                                                                                                                                   <  1:249240/251‑1 (MQ=255)

                                                             aTACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTc                                                                                                                                                                                  >  1:530445/1‑251 (MQ=255)

                                                                 cTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTgcc                                                                                                                                                                              >  1:60955/1‑251 (MQ=255)

                                                                 cTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTgcc                                                                                                                                                                              >  2:228323/1‑251 (MQ=255)

                                                                     ggCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTgccgcc                                                                                                                                                                           >  1:364527/1‑250 (MQ=255)

                                                                      gcgggggCGTCGGGTTCGACAATGCTGCGGGTGATATCGATGGGGCCGCCTTTGCGCGCGAATTCTAGAGCCTGCTCAAAAAACG‑GTACGTTGCGGTTAACTTGTGGT‑GGAAGCAGCTTGCTAATAGG‑CACATCGCAGTTTTCCAGCAGGTCGTAAATAGGCTGTAACGCCATTTTATTGTCGCCCAGGCGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAGCCGCCAACGCGGGAGGCTGCCGCCat                                                                                                                                                                         <  2:431088/248‑1 (MQ=255)

                                                                           ggCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTgg                                                                                                                                                                    >  1:199222/1‑251 (MQ=255)

                                                                            gCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTCCGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGc                                                                                                                                                                   <  1:253200/251‑1 (MQ=255)

                                                                              gACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGcc                                                                                                                                                                  >  1:371104/1‑250 (MQ=255)

                                                                              gACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGcc                                                                                                                                                                  >  1:96185/1‑250 (MQ=255)

                                                                              gACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGcc                                                                                                                                                                  >  1:557270/1‑250 (MQ=255)

                                                                                 cGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGt                                                                                                                                                              >  1:106721/1‑251 (MQ=255)

                                                                                  ggTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTg                                                                                                                                                             >  2:455628/1‑251 (MQ=255)

                                                                                      cGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATaa                                                                                                                                                         <  1:318327/251‑1 (MQ=255)

                                                                                           aTGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTc                                                                                                                                                    >  1:193860/1‑251 (MQ=255)

                                                                                             gctgctGGTGATATCGATGGTGCCGCCTTTGCGCGCGACCTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTcc                                                                                                                                                   <  1:118298/250‑1 (MQ=255)

                                                                                             gctgctGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGCGATAAACGTCCg                                                                                                                                                  >  2:337464/1‑251 (MQ=255)

                                                                                             gctgctGGTGATATCGATGGTGCCGCCTTTGCGCGCAAACTCAAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGAAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTcc                                                                                                                                                   <  1:274903/250‑1 (MQ=255)

                                                                                             gctgctGGTGATATCGATGGTGACGCCTTTGCGCGCGAAATCCAGCGCCTGATCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACACCGCAGTTTTACAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCg                                                                                                                                                  >  2:433812/1‑251 (MQ=255)

                                                                                              ctgctgGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCgg                                                                                                                                                 >  1:416391/1‑251 (MQ=255)

                                                                                                gctgGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCgg                                                                                                                                                 <  1:187423/249‑1 (MQ=255)

                                                                                                  tgGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGcc                                                                                                                                                                                              >  1:326770/1‑202 (MQ=255)

                                                                                                  tgGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCg                                                                                                                                             >  1:97080/1‑251 (MQ=255)

                                                                                                  gggTGATATCGATGGTGCCGCTTTTGCGCGCTAACCCCACCGCTGGCTCAAACACCG‑GTACGTGGGGGTTACCGTG‑GGCCGGCAGCAGCTGGCGGATCGG‑CACATCGCAGTTTTCAAGCAGGTCAAAAATAGGCTGAAACTCCATTTTACTGTCGCCCAGTTGGAACCCGG‑TGAC‑GCCAGGTTGACCGCGGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCg                                                                                                                                             <  2:548981/250‑1 (MQ=255)

                                                                                                  gggTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGcc                                                                                                                                                                                              <  2:326770/201‑1 (MQ=255)

                                                                                                    gTGATAGCGAGGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCATTTTTCCAGCAGGTCATAAATAGGCTGTACCGCCATTTTCCTGTCGCCCTTGTGGACCACGG‑TGAC‑GCCAGTTTTACCGCCGGGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGc                                                                                                                                           <  2:474366/251‑1 (MQ=255)

                                                                                                            gATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGGG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGt                                                                                                                                   <  2:530445/251‑1 (MQ=255)

                                                                                                               ggTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGa                                                                                                                                 <  1:366025/250‑1 (MQ=255)

                                                                                                                  gccgccTTTGCGCGCGACCTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGGTCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCag                                                                                                                             <  2:95853/251‑1 (MQ=255)

                                                                                                                  gccgccTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATc                                                                                                                               >  1:530078/1‑249 (MQ=255)

                                                                                                                     gccTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTCCGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CCCATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTACCGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAgaga                                                                                                                          <  2:584524/251‑1 (MQ=255)

                                                                                                                            cgcgcgAACTCCCGCCCCTGCTCAAACAGCGCGT‑CGTTGGGGTTAACGTG‑GGCCGGCAGCAGCTTG‑TGGTCGGGCCCATCGCAGTTTGCCTGCAGGCCATAAATGGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACCCGG‑TGAC‑GCCAGTTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGTTGATCAGAGATGGCGCa                                                                                                                   <  2:190380/251‑1 (MQ=255)

                                                                                                                             gcgcgAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCAt                                                                                                                  <  1:482748/251‑1 (MQ=255)

                                                                                                                              cgcgcaccccAGCGCCGGATCAAACAACG‑GGACTTTGCGGTTAACGGG‑GGGCGGCAGCAGCTGGCTGGTCGG‑CGCATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTCCTGTCGCACATGTGGAACACGG‑TGAC‑GCCATGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGACAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCAtt                                                                                                                 <  2:570739/243‑1 (MQ=255)

                                                                                                                              cgcgAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCAtt                                                                                                                 >  1:140962/1‑251 (MQ=255)

                                                                                                                              cacgacccccAGCTCCTGCACAAACAAGG‑GTACGTGGCGGTTAACGGG‑GGTGGGCAGCAGCTTGCTGATCGG‑CACAGCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGTTTTACCGCCGAGCAAACCGCCACCGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCAtt                                                                                                                 <  2:145533/243‑1 (MQ=255)

                                                                                                                                 gAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTc                                                                                                               >  1:335355/1‑250 (MQ=255)

                                                                                                                                        aGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGGGATAAACGTCCGGTGCGGCAGGACGGTGATCAGAGATGGCGCATTTCACGCCAAt                                                                                                       >  2:263391/1‑251 (MQ=255)

                                                                                                                                         gcgcCTGCTCAAACAACG‑GTCCGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATc                                                                                                      <  1:142141/251‑1 (MQ=255)

                                                                                                                                                 tCAAACATCG‑GTACGTTGCGTTTAACGTG‑GGTGGCCAGCGGCTTGCTGACCGG‑CTCATCGCAGTTTCCCAGAAGGTCATAAATAGGCTGTAACGCCATTTTACGGTCGCCCAGGGGGAACACGG‑TGCC‑GCTGGGTTTCCCGCCGAGCAAACCGCCACCGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACAcgaa                                                                                               <  2:48483/250‑2 (MQ=255)

                                                                                                                                                    aacaacG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACAcgatcgat                                                                                           >  1:532813/1‑251 (MQ=255)

                                                                                                                                                     acaacG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACAcgatcgat                                                                                           >  1:454638/1‑250 (MQ=255)

                                                                                                                                                       aacG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAAt                                                                                        <  1:304783/251‑1 (MQ=255)

                                                                                                                                                       aacG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAAt                                                                                        >  1:298568/1‑251 (MQ=255)

                                                                                                                                                         cG‑GTGCGTTGCGGTTAACGTG‑GGTCGGAAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTACAGCAGGTCATAAAGAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAAGACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAGCGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGTTCGATAATCg                                                                                      <  2:420876/251‑1 (MQ=255)

                                                                                                                                                          g‑gTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGc                                                                                     >  1:119131/1‑251 (MQ=255)

                                                                                                                                                              acggtccgtttgaCGTG‑GGTCGGCAGCAGCTTGTTGAGCGG‑CACATCTCAGTTTTCCAGCGGCTCATAAATAGGCTGTACCGCCATTTTCCTGCCCCCCATGTGGA‑CCCCG‑TGACCGCCAGTTTTACCGCCTAGCAACCCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCAc                                                                                  <  2:216005/239‑1 (MQ=255)

                                                                                                                                                                gTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTCACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGACATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATAGCCACGt                                                                                >  2:248026/1‑251 (MQ=255)

                                                                                                                                                                  tGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGACCGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCt                                                                              >  2:470055/1‑251 (MQ=255)

                                                                                                                                                                     ggTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCtttt                                                                           >  1:448373/1‑251 (MQ=255)

                                                                                                                                                                         aaCGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTcc                                                                        <  1:86469/250‑1 (MQ=255)

                                                                                                                                                                         aaCGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTcc                                                                        >  1:585926/1‑250 (MQ=255)

                                                                                                                                                                           cGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACg                                                                     >  2:192142/1‑251 (MQ=255)

                                                                                                                                                                                   cGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCg                                                              <  1:455628/251‑1 (MQ=255)

                                                                                                                                                                                    ggCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGt                                                             >  2:528718/1‑251 (MQ=255)

                                                                                                                                                                                      cagcagCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTa                                                            >  2:155191/1‑250 (MQ=255)

                                                                                                                                                                                      cagcagCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTa                                                            >  1:502556/1‑250 (MQ=255)

                                                                                                                                                                                       ggcggcTTGCTGTTCGG‑CACATCGCAGTTTTCCAGCGGGTCATAAAGAGGCTGTAACGCCATTTTACGGTCGCCCATGGGGAACCCGG‑TGAC‑GCAAGGTTTACCGCCGAGCAAACCGCCACCGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAAt                                                          <  2:193860/247‑1 (MQ=255)

                                                                                                                                                                                       ggcagttttctgtgcGG‑CACATCGCAGTGTTCCAGCAGGTAATAAATAGGATGTAACGCCATTTTACTGTCGCCCATGGGGACCACGG‑GGAC‑GCCGGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAAt                                                          <  2:96185/237‑1 (MQ=255)

                                                                                                                                                                                                          ccaTCGCAGTTTTCCAGCAGGTCATAAATAGGCGGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTCCCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATg                                        <  1:497115/250‑1 (MQ=255)

                                                                                                                                                                                                            aTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGAt                                      >  1:220702/1‑251 (MQ=255)

                                                                                                                                                                                                                cAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAgc                                  >  2:469110/1‑251 (MQ=255)

                                                                                                                                                                                                                 aGTTTTCAAGCAGGTCATAAATAGGCGGTAACGCCTTTTGACTGTCGCCAATGTGGAACACGG‑TGAC‑GCCAGGTGTACCGCCGAGCAAACCGCCAACGGGGGATTCTGCCGCCATAGTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAAGCACACTATCTATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAgcg                                 <  1:516415/251‑1 (MQ=255)

                                                                                                                                                                                                                         ggcgggacataaagaggatttcaCGCCTTTTTACTGGCACCAGTGTGGAA‑ACGGGTGAC‑GCCTGTTTTACCGCCGAGCAAACCCCCAACGCGGGTTTCTGCCGCCATATTTGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAg                         <  2:572905/229‑1 (MQ=255)

                                                                                                                                                                                                                         agcagGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCGTGTGGAACACGG‑TGAC‑GCCGGGTTTACCGCCGAGCAAACCGCCAGCGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAg                         <  2:633/251‑1 (MQ=255)

                                                                                                                                                                                                                         agcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAg                         <  1:31856/251‑1 (MQ=255)

                                                                                                                                                                                                                            agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCGGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCAt                      <  1:483816/251‑1 (MQ=255)

                                                                                                                                                                                                                            agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCAt                      <  1:228323/251‑1 (MQ=255)

                                                                                                                                                                                                                                    aaTAGGCTGTAACGCCATTTTACTGTCGCCCACGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGAGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGAGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGgcgc              >  1:110402/1‑251 (MQ=255)

                                                                                                                                                                                                                                     aTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGGCGCt             <  1:110682/251‑1 (MQ=255)

                                                                                                                                                                                                                                            gTAACGCCATTTTACTGTCGCCCATGTGGAACCCGG‑TGAC‑GCCAGGTTTCCCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCct      <  1:48128/251‑1 (MQ=255)

                                                                                                                                                                                                                                                cGCCATTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCcttctt  <  1:469110/251‑1 (MQ=255)

GTTGCCGTCGGAGCTGAGGGTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACG‑GTACGTTGCGGTTAACGTG‑GGTCGGCAGCAGCTTGCTGATCGG‑CACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGG‑TGAC‑GCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTT  >  NC_000913/4558666‑4559153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`