Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,313,027	T→G	intergenic (‑36/‑323)	alsR ← / → rpiB	DNA‑binding transcriptional repressor AlsR/allose‑6‑phosphate isomerase/ribose‑5‑phosphate isomerase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,313,027	0	T	G	100.0%	127.5 / NA	35	intergenic (‑36/‑323)	alsR/rpiB	DNA‑binding transcriptional repressor AlsR/allose‑6‑phosphate isomerase/ribose‑5‑phosphate isomerase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (13/22);  total (13/22)

CTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTA  >  NC_000913/4312785‑4313266                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                cTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATcaccac                                                                                                                                                                                                                                         >  1:136578/1‑251 (MQ=255)     gATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGc                                                                                                                                                                                                                                      <  2:566796/250‑1 (MQ=255)             accTATCATAGCTTCCGATACCGCCAGAGCTTTTGCGACATCTTTCATTCCGGGTGCACAACTCAGGTTACCGGGGTTGAGTACCCCCTCCCCGATGCGGCTTTCATTTTCTGCCATTCCTTCCTGCTTCCTTCGCAGGTAAGGCGCTAACCCTATACCGTTCAGAAGCGCTGACTCAAAATCTGACTGGCTCATCACGTTCTCTTGTCCTTGACTTGAAGACGACACTAGATCACCACAGCAAGAAc                                                                                                                                                                                                                                >  2:432610/3‑248 (MQ=255)             aaCTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACATCTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCTCCACAGCAATAACAg                                                                                                                                                                                                                              <  2:185738/250‑1 (MQ=255)                   catcGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGAt                                                                                                                                                                                                                       <  1:250135/251‑1 (MQ=255)                      cGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCt                                                                                                                                                                                                                    <  2:430970/251‑1 (MQ=255)                       gCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTg                                                                                                                                                                                                                   >  1:127338/1‑251 (MQ=255)                           cAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGGCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAGAGGATGCTGGCg                                                                                                                                                                                                                <  2:165104/250‑1 (MQ=255)                               tACCGCAAGAGCTTCTGGGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCAAGCTGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAATGCGCTAACCCTATACCGTTCGGAAGCGCTGTATCAAACTCTGACTGGCTCATCACGCTCTCTTGTCCTTGAATTGAAGACGCCACTCGATCACCCAAGTAAGAACCGAAAAGATGCTGGCgcattc                                                                                                                                                                                                           >  2:526776/1‑246 (MQ=255)                                aCCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTc                                                                                                                                                                                                           <  1:405191/250‑1 (MQ=255)                                 ccGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTcac                                                                                                                                                                                                         <  1:15167/251‑1 (MQ=255)                                   gCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGGATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACaa                                                                                                                                                                                                       <  1:194029/251‑1 (MQ=255)                                       gagCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACaaaaaa                                                                                                                                                                                                   <  1:146746/251‑1 (MQ=255)                                                         tAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACATAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGtt                                                                                                                                                                                  <  1:25116/250‑1 (MQ=255)                                                          aaTTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGttt                                                                                                                                                                                 <  1:354622/250‑1 (MQ=255)                                                              gCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGtttattt                                                                                                                                                                             <  1:443945/250‑1 (MQ=255)                                                                    gCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATtgtg                                                                                                                                                                      <  2:90384/251‑1 (MQ=255)                                                                         aCTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACa                                                                                                                                                                  >  1:231385/1‑250 (MQ=255)                                                                               gTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTCACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCg                                                                                                                                                            >  1:511705/1‑250 (MQ=255)                                                                                                aCCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAAGGGCGAGTGGCTGAAAATTTTAt                                                                                                                                          <  2:502622/251‑1 (MQ=255)                                                                                                   aCTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCGGCTTCATTCGCAGGTAAGGCGCTAACCCTACTCCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTACTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTc                                                                                                                                       <  1:174622/251‑1 (MQ=255)                                                                                                            tGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAAtt                                                                                                                               >  1:545667/1‑250 (MQ=255)                                                                                                                     cATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTaaaa                                                                                                                      >  1:570494/1‑250 (MQ=255)                                                                                                                      aTTTTCTGTCATTCCTGCCTGCTTCATTCGCATGGAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACTTTCTCTTGTCCTTGAATTGAATACGCCACTAGAGCACCACAGCAAGAACAGAAAAGATGGTGGCGACTACACAAAAAAATAAAATTTATAAAGTTTATTTTATTATGAACAATGGCGAGTGGCTTAAAATTTTATTTCAGTTatttggtataaagac                                                                                                                    <  2:397339/251‑15 (MQ=255)                                                                                                                                       cctGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGtttt                                                                                                   <  2:350139/251‑1 (MQ=255)                                                                                                                                       cctGCTCCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGttt                                                                                                    <  2:234558/250‑1 (MQ=255)                                                                                                                                         ggctgtaTTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACCCTGACTGGCTCAACACGTTCTCTTGTAATGGAATTGAAGACGCCACTAGATCACCATATCAAGAACAGAAAAGATGGTGGCGGCTTCACAAAAAAATAAAATTTATTTAGTTTATTTTATTGTGAATAATGGCGAGTGGCTTAATATTTTATTTCATTTAATTAGTTTAAAACCATGATGTTATTTGTTTTTc                                                                                                 <  2:65454/245‑1 (MQ=255)                                                                                                                                                           ggCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGAACACCACAGCAAGAACAGAAAAGATGCTGGCGAATTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGTTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTGTTCTGGTTTTTTAgatgatcg                                                                               <  2:331530/251‑3 (MQ=255)                                                                                                                                                                 aaCCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGtt                                                                          <  2:481780/250‑1 (MQ=255)                                                                                                                                                                            gTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGt                                                                                                             >  2:319707/1‑204 (MQ=255)                                                                                                                                                                                                     gACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTc                                     >  2:213737/1‑251 (MQ=255)                                                                                                                                                                                                       cTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTc                                     >  1:543445/1‑249 (MQ=255)                                                                                                                                                                                                                             ccTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGAt              <  1:2008/250‑1 (MQ=255)                                                                                                                                                                                                                              cTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATc             >  2:47868/1‑250 (MQ=255)                                                                                                                                                                                                                                         gACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTa  >  2:342659/1‑250 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                CTTTGATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTA  >  NC_000913/4312785‑4313266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑