Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,312,289	A→G	Y235H (TAT→CAT)	alsR ←	DNA‑binding transcriptional repressor AlsR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,312,289	0	A	G	100.0%	161.2 / NA	44	Y235H (TAT→CAT)	alsR	DNA‑binding transcriptional repressor AlsR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/20);  total (24/20)

TAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCG‑CCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCA‑GCAAAGTTAGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGAT  >  NC_000913/4312042‑4312529                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                 tcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCG‑CCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCA‑GCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCg                                                                                                                                                                                                                                              >  1:185317‑M2/16‑251 (MQ=255)                      gggACAG‑TAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCG‑CCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCA‑GCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGa                                                                                                                                                                                                                          >  1:381647/1‑250 (MQ=255)                             gTAACTCCGCGATTGCGGAATTACCTTTTTCTTATTTCAGCGCG‑CCTGGTGAGAAGAAATCAACAATTGCGACGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCA‑GCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAg                                                                                                                                                                                                                   <  2:438450/251‑1 (MQ=255)                                     gcgATGGCGGAATTTCCTTTTTCTTATTGCAGCGCG‑CCAGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCAGGGCGACAGAGACGAAAAAAGCGTCCA‑GCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTGCGCTGTCGGTGAATGGTAGCTATGGGt                                                                                                                                                                                                           <  2:204691/251‑1 (MQ=255)                                                    ccTTTTTCTTATTTTAGCGCGGCCTG‑TGAGGAGGAATCCACAATTTCGCCGGTTTTTTCCATATTAATAATAGCTTGTTCAATGTTGAGCTGGGGGACAGGAACAAAAAAAGCGTCCA‑GCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTAGGGGTTATACAAATAATCt                                                                                                                                                                                             <  2:518494/250‑1 (MQ=255)                                                             tATTTCAGCGCG‑CCTGGTGAGAAGAAATCAACAATTGCGCTGGTTTTTTCCATATTACTATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAACAAAGCGTCCA‑GCAAGGTTGGTT‑GTAATATTCTTGACGAGGCATTAAGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGCTCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGtt                                                                                                                                                                                   >  2:83762/1‑251 (MQ=255)                                                                   aGCGCG‑CCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCA‑GCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCttttt                                                                                                                                                                             <  2:12206/251‑1 (MQ=255)                                                                     cgcg‑cCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCA‑GCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTAAGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTg                                                                                                                                                                           >  2:52043/1‑251 (MQ=255)                                                                                     gaaATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCA‑GCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGAAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCg                                                                                                                                                            >  1:259513/1‑251 (MQ=255)                                                                                         tCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCA‑GCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCttt                                                                                                                                                        >  2:388671/1‑251 (MQ=255)                                                                                           aacaaTTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCA‑GCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCtttt                                                                                                                                                       >  1:217614/1‑250 (MQ=255)                                                                                              aatggggcCGGTTTTTTCCATATAAATATTAGCTGGTTCAAGGTTGAGCTGGGCGAAAGAGACAAAAAAAGC‑TCCAAGAAAGGTTGGTT‑GTAATATCCTGGCGGAGGAATTCCGCCCTAA‑AACGGGCGTTTCCGGGGCTGGTGGGCAAAAAATATGACGGGCCGGTTCGGCTACCGTTGAATGGTAGCAATGGGT‑ATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATc                                                                                                                                                  <  2:185317/245‑1 (MQ=255)                                                                                                    gcCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCA‑GCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTgg                                                                                                                                             >  1:293748/1‑251 (MQ=255)                                                                                                                 atattaatattaGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCA‑GCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATg                                                                                                                                >  2:143143/1‑251 (MQ=255)                                                                                                                 atattaatattaGCTTGATCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCA‑GCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCGATGGGTTATACAAATAATCGTTGCCCGGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATg                                                                                                                                >  1:567889/1‑251 (MQ=255)                                                                                                                         attaGCTTGTTCAATGTTGAGCTGGGCGCCAGAGACAAAAAAAGCGTCCA‑GCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAACTAATATGATCAGCCAGTTTCGCTATCGGTGAATGGTAGCGATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGCATGGGTCACt                                                                                                                         >  2:204700/1‑250 (MQ=255)                                                                                                                            aGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCA‑GCAAGGTTGGTT‑GTAATATTCTTGCCGAGGTGTTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTAcc                                                                                                                      <  1:204700/250‑1 (MQ=255)                                                                                                                                    cAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCA‑GCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCacaac                                                                                                              <  2:51311/250‑1 (MQ=255)                                                                                                                                     aaTGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCA‑GCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACAt                                                                                                            <  2:323640/251‑1 (MQ=255)                                                                                                                                       tggtGAGCTGGGCGACAGAGACAAAAAAAGCGTCCA‑GCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACAtct                                                                                                          >  2:66980/4‑251 (MQ=255)                                                                                                                                        gTTGAGCTGGGCGACAGAGACAAAAAACGCGTCCA‑GCACGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTCCGGCCGATTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACAtctc                                                                                                         >  2:204102/1‑251 (MQ=255)                                                                                                                                           gAGCTGGGCGACAGAGACAAAAAAAGCGTCCA‑GCAAGGTTGGTT‑GTAATATTCTTGCCGATGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCtccgt                                                                                                      <  1:558061/251‑3 (MQ=255)                                                                                                                                           gAGCTGGGCGACAGAGACAAAAAAAGCGTCCA‑GCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAAt                                                                                                                                                                                                                        <  1:318210/137‑1 (MQ=255)                                                                                                                                                  gCGACAGAGACAAAAAAAGCGTCCA‑GCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTac                                                                                               >  2:333511/1‑250 (MQ=255)                                                                                                                                                   cgaaagagAAAAAAAAAGCGGCCA‑G‑TAAGTTTGTTTGTAATATTCTTGCCGAGGCATTACGACCTACTATCGG‑CGTTGCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTTAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTGTTACAGCTCTGGTTCGCCCGGAATGGGTCACTCCCAGCACACCATCTCCTTCCTGTaac                                                                                              <  2:573989/247‑1 (MQ=255)                                                                                                                                                    gACAGAGACAAAAAAAGCGTCCA‑GCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGGATGGGTCACTACCAGCACAACATCTCCTTCCTGTaac                                                                                              >  2:64444/1‑250 (MQ=255)                                                                                                                                                           aCAAAAAAAGCGTCCA‑GCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGc                                                                                      >  1:53803/1‑251 (MQ=255)                                                                                                                                                               aaaaaGCGTCCA‑GCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCgg                                                                                                                                                                                                                             <  1:273923/112‑1 (MQ=255)                                                                                                                                                               aaaaaGCGTCCA‑GCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCgg                                                                                                                                                                                                                             >  2:273923/1‑112 (MQ=255)                                                                                                                                                                   aGCGTCCA‑GCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACACCGAAGCGGAcatc                                                                               >  2:72153/1‑250 (MQ=255)                                                                                                                                                                    gCGTCCA‑GCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGAcatcat                                                                             <  1:143143/251‑1 (MQ=255)                                                                                                                                                                    gCGTCCA‑GCAAGGTTGGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGAcatcat                                                                             <  1:537251/251‑1 (MQ=255)                                                                                                                                                                                    ggtt‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATc                                                              >  1:65773/2‑251 (MQ=255)                                                                                                                                                                                                  ttGCCGAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGACGCGGACATCATCATGATGTGAGCATCAGGATAGGcatgc                                                 >  2:15040/1‑247 (MQ=255)                                                                                                                                                                                                       gAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTGTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCg                                            >  1:528582/1‑251 (MQ=255)                                                                                                                                                                                                         ggCATTACGACCTAATAACGG‑CGTTTCCGGGTCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACATCATCTCCTTCCTGTAACAACGAAGGGGACATCATCATGATGTGAGCATCAGGATAGGCCTAACAGCGTa                                          <  2:207818/251‑1 (MQ=255)                                                                                                                                                                                                             ttACGACCTAATACCGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCTGCCAGTTTCGCTATCGGTGAATGGTAGCTTTGGGTTATACAAATAATCTGTGCCCCGTTCTTTTTTGCCAGTTCGACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTGCTGTAACACCGAAGCGGACATCATCATGATGTGTGCATCAGCTTAGGCCTGACatcggacgt                                       <  2:421563/250‑9 (MQ=255)                                                                                                                                                                                                                          atcgtcGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTTAATGGTCGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACCGCGTACGCCAATGGGCAAGa                           <  2:433245/245‑1 (MQ=255)                                                                                                                                                                                                                          aCGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAAc                         <  1:388671/251‑1 (MQ=255)                                                                                                                                                                                                                                             gTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGc        >  1:397339/1‑250 (MQ=255)                                                                                                                                                                                                                                               gAGCAAATAATATGATCGGCGAGTTGCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCTGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAATAACTTGTGCTGTACAGCAGcaca     <  2:303741/251‑1 (MQ=255)                                                                                                                                                                                                                                               gAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGcac      >  1:262974/1‑250 (MQ=255)                                                                                                                                                                                                                                                aGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAg    <  1:204102/251‑1 (MQ=255)                                                                                                                                                                                                                                                    aataatATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGAt  >  1:185738/1‑249 (MQ=255)                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                 TAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCG‑CCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCA‑GCAAAGTTAGTT‑GTAATATTCTTGCCGAGGCATTACGACCTAATAACGG‑CGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGAT  >  NC_000913/4312042‑4312529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑