Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,256,0610CT29.1% 75.7 / 16.5 55E136K (GAA→AAA) plsBglycerol‑3‑phosphate 1‑O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (6/33);  new base T (16/0);  total (22/33)
Fisher's exact test for biased strand distribution p-value = 2.51e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGAT‑AGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCC‑TTTTTC‑ACGCCCC‑GG‑CGCGCGACCAAAC‑ATC‑ACCGACA‑CTGGCACCATCTGCACATCCAGATTT‑GGGTTG‑CTACGGTGCAAATCGAGATAGTCG‑TGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAAT‑AGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAA  >  NC_000913/4255813‑4256301
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                          
attaAACAGCTCCTGACGCGCAGGAAAACGTGGGCATAGGGCAGCCAGCCGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGGTTCTGAGCGAT‑AGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCC‑TTTTTC‑Ac                                                                                                                                                                                                                                                         <  2:35030/251‑1 (MQ=255)
attaAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGAT‑AGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCC‑TTTTTC‑Ac                                                                                                                                                                                                                                                         <  1:410790/251‑1 (MQ=255)
                  aGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGAT‑AGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCC‑TTTTTC‑ACGCCCC‑GG‑CGCGCGACCaa                                                                                                                                                                                                                                     <  1:332056/251‑1 (MQ=255)
                          aaCGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGAT‑AGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCCCCCTTTTTT‑A‑GCCCC‑CG‑CGCGCGACCAAAA‑ATC‑Acc                                                                                                                                                                                                                           >  1:337453/1‑251 (MQ=255)
                             gggggcatggggCAGCCAGCCGTGGACGGGCAAGGTGCATCCGGGCCCCGCGCGCGAGTTTCTGAGCGAT‑AGTTTTATCCGTGCCGGGTTCACCCGCCATACGGCGCAGCGAAACGGACGGCGAGAAACGCACAAAACTGGCGCGACCGAGCCACAGTACAGCGAAAAATTGCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCC‑TTTTTC‑ACGCCCC‑GG‑CGCGCGACCAAAC‑ATC‑ACCGac                                                                                                                                                                                                                        <  2:508827/241‑1 (MQ=255)
                                ggCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGAT‑AGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCCATTCGCC‑TTTTTT‑AACCCCC‑GC‑CCCGCGACCCAAC‑ATC‑ACCgcaccc                                                                                                                                                                                                                     >  2:237487/1‑246 (MQ=255)
                                    gacgtcgtcgtaccGTTGAGGGGCAAAGTGCATCCGCGCCACGCGGGCCAGTTGCTGAG‑GATGATTTTTTTCCGTGCCATGTTCATCCGCCATACGGCGCATCGAAACCGACGGCGAGAAACGCACAAATCTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCC‑TTTTTC‑ACGCCCC‑GG‑CGCGCGACCAAAC‑ATC‑ACCGACA‑CTGGc                                                                                                                                                                                                                 <  2:327939/237‑1 (MQ=255)
                                         cAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGAT‑AGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCC‑TTTTTC‑ACGCCCC‑GG‑CGCGCGACCAAAC‑ATC‑ACCGACA‑CTGGCACCATc                                                                                                                                                                                                           <  1:337696/251‑1 (MQ=255)
                                           gCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGAT‑AGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCC‑TTTTTC‑ACGCCCC‑GG‑CGCGCGACCAAAC‑ATC‑ACCGACA‑CTGGCACCATCTg                                                                                                                                                                                                         <  1:97427/251‑1 (MQ=255)
                                                    gggcggtaaaagtgctaccgcgCCCCGCGCGCCATTTTCTGCGCGAT‑GGTTTTCTCCGTGCCGGTTTCACCCGCCAGCCGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGCCCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCC‑TTTTTC‑ACGCCCC‑GG‑CGCGCGACCAAAC‑ATC‑ACCGACA‑CTGGCACCATCTGCACATCCAg                                                                                                                                                                                                <  2:367543/233‑1 (MQ=255)
                                                        gggCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGGTAT‑AGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAACCTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCC‑TTTTTC‑ACGCCCC‑GG‑CGCGCGACCAAAC‑ATC‑ACCGACA‑CTGGCACCATCTGCACATCCAGAttt                                                                                                                                                                                            <  1:581128/251‑1 (MQ=255)
                                                          gCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGAT‑AGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCCCCTTTCC‑TTTTTT‑TCCCCCC‑GG‑CGCGCGCCCACAA‑ATC‑ACCCACA‑CCTGCCCCCCCTGCACATCCaacttttg                                                                                                                                                                                          >  1:557038/1‑244 (MQ=255)
                                                           cAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGAT‑AGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCCCCTCGCC‑CTTTTT‑ACCCCCC‑CG‑CCGGCCGCCAAAC‑CTC‑ACCCACA‑CTCGCAccctcctgacccacagaatttgg                                                                                                                                                                                         >  2:197515/1‑230 (MQ=255)
                                                            aaaGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGAT‑AGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCC‑TTTTTC‑ACGCCCC‑GG‑CGCGCGACCAAAC‑ATC‑ACCGACA‑CTGGCACCATCTGCACATCCAGATTT‑GGGt                                                                                                                                                                                       <  1:200885/251‑1 (MQ=255)
                                                             aattGCATACGCGCCACGCGCGCCAGTTTCTGAGCGAT‑ATTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCC‑TTTTTC‑ACGCCCC‑GG‑CGCGCGACCAAAC‑ATC‑ACCGACA‑CTGGCACCATCTGCACATCCAGATTT‑GGGtt                                                                                                                                                                                      <  1:392949/248‑1 (MQ=255)
                                                             aaGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGAT‑AGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTTAATTCGCC‑TTTTTT‑ATGCCCC‑CG‑CGCGCGACCAAAC‑ATT‑AAACACAACGGGCAC‑ACCTGCACAGCCAGATTT‑GGGtt                                                                                                                                                                                      >  1:137548/1‑251 (MQ=255)
                                                                 gCATCCGCGCCTCGCGCGCCAGTTTCTGAGCGAT‑AGTTTTACCCGGGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCC‑TTTTTC‑ACGCCCC‑GG‑CGCGCGACCAAAC‑ATC‑ACCGACA‑CTGGCACCATCTGCACATCCAGATTT‑GGGTTG‑CTa                                                                                                                                                                                 <  1:45155/251‑1 (MQ=255)
                                                                           cacgctggcCAGTTTCTGAGCGAA‑ATTTTGACCCGTGCCGTGTCCAGCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTTTCGCGGCCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCC‑TTTTTC‑ACGCCCC‑GG‑CGCGCGACCAAAC‑ATC‑ACCGACA‑CTGGCACCATCTGCACATCCAGATTT‑GGGTTG‑CTACGGTGCAAAt                                                                                                                                                                       <  1:154166/244‑1 (MQ=255)
                                                                               cgcgcCAGTTTCTGAGCGAT‑AGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACCTCGCC‑TTTTTC‑ACGCCCC‑GG‑CGCGCGACCCAAA‑ATC‑ACCGACA‑CTGGCCCCCTCTGCACATCCAGATTT‑GGGTTG‑CTACGGGGCAAATCg                                                                                                                                                                     >  1:376045/1‑249 (MQ=255)
                                                                                   ccAGTTTCTGAGCGAT‑AGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCC‑TTTTTC‑ACGCCCC‑GG‑CGCGCGACCAAAC‑ATC‑ACCGACA‑CTGGCACCATCTGCACATCCAGATTT‑GGGTTG‑CTACGGTGCAAATCGAGATAg                                                                                                                                                               <  1:367684/251‑1 (MQ=255)
                                                                                      gTTTCTGAGCGAT‑AGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTTACTTCGCC‑CTTTTC‑ACGCCCC‑CG‑CGCGCGACCAAAC‑ATC‑ACCGCCA‑CTCGCACCACCTGCACATCCCGATTT‑GGGTTG‑CTTCGGTGCAAATCGAGATAGTCg                                                                                                                                                            >  1:406523/1‑251 (MQ=255)
                                                                                          cTGAGCGAT‑AGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCTCC‑TTTTTT‑ACGCCCC‑CG‑CGCGCGACCAAAC‑ATC‑CCCCACA‑CTGGGCCCATCTGCCCATCCAGAATT‑GGGGTG‑CTACGGGGCAAATCGGGATAGTCG‑Tggg                                                                                                                                                       >  1:189008/1‑250 (MQ=255)
                                                                                             gtcGAT‑AGTGTAATCCGGGCCGTGTTCAGCGGCCATCCGGCGCAGCGAAACTGACGGCGAGAAAGGCACAAAGCGGGGGCGACGGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCAGACGCAGCGGCGGGTGAAATTCGGC‑TTTTTC‑ACGCCCC‑GG‑CGCGCGACCAAAC‑ATC‑ACCGACA‑CTGGCACCAGCTGCACATCCAGATTT‑GGGTTG‑CTACGGTGCAAATCGAGATAGTCG‑TGGAACa                                                                                                                                                    <  1:246088/249‑1 (MQ=255)
                                                                                                     gTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCC‑TTTTTC‑ACGCCCC‑GG‑CGCGCGACCAAAC‑ATC‑ACCGACA‑CTGGCACCATCTGCACATCCAGATTT‑GGGTTG‑CTACGGTGCAAATCGAGATAGTCG‑TGGAACAGCTTAAt                                                                                                                                             <  1:215047/251‑1 (MQ=255)
                                                                                                       ttacccggtcctgtttgaacccGCAATAGGGCACAGCGAAATTGACGGAGAAAAACGCAAAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCC‑TTTTTC‑ACGCCCC‑GG‑CGCGCGACCAAAC‑ATC‑ACCGACA‑CTGGCACCATCTGCACATCCAGATTT‑GGGTTG‑CTACGGTGCAAATCGAGATAGTCG‑TGGAACAGCTTAATa                                                                                                                                            <  2:295772/230‑1 (MQ=255)
                                                                                                        tttcccgtgccgtgTACACCCGCCATACGGCGCAGCGAATCTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCC‑TTTTTC‑ACGCCCC‑GG‑CGCGCGACCAAAC‑ATC‑ACCGACA‑CTGGCACCATCTGCACATCCAGATTT‑GGGTTG‑CTACGGTGCAAATCGAGATAGTCG‑TGGAACAGCTTAATAg                                                                                                                                           <  2:137548/246‑1 (MQ=255)
                                                                                                          aTCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCC‑CTTTTT‑TCGCCCC‑CG‑CGCGCGACCAAAC‑ATC‑ACCGACA‑CCGGCCCCCCCTTCACATCCCGATTT‑TGGTTT‑CTACGGTGCAAAACTAGATAGTCG‑TGGAACAACTTAATATAct                                                                                                                                        >  1:30982/1‑251 (MQ=255)
                                                                                                          aTCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCTCC‑TTTTTT‑CCGCCCC‑GG‑CGCGCGCCCAAAC‑ATC‑ACCGCCA‑CTGGCACCCTCTCCACATCCACATTT‑GGGGTG‑CTTCGGGGCAAATCGCGATAATCG‑TGGAACAGCTTATTAGAct                                                                                                                                        >  2:379555/1‑251 (MQ=255)
                                                                                                                gccgtgTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCC‑TTTTTC‑ACGCCCC‑GG‑CGCGCGACCAAAC‑ATC‑ACCGACA‑CTGGCACCATCTGCACATCCAGATTT‑GGGTTG‑CTACGGTGCAAATCGAGATAGTCG‑TGGAACAGCTTAATAGACTcttctt                                                                                                                                  <  1:120517/251‑1 (MQ=255)
                                                                                                                cccgtgtcccgccGCCCTACGGCGCAGAGAACCTGACGGCGAGAAACGCACAAAACTGCCGGGACCGAGCCACAGTACAGCGAAAAAGTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCC‑TTTTTC‑ACGCCCC‑GG‑CGCGCGACCAAAC‑ATC‑ACCGACA‑CTGGCACCATCTGCACATCCAGATTT‑GGGTTG‑CTACGGTGCAAATCGAGATAGTCG‑TGGAACAGCGTAATAGACTcttctt                                                                                                                                  <  2:160616/240‑1 (MQ=255)
                                                                                                                      ttCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGCACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCC‑TTTTTT‑ACGCCCC‑GG‑CGCGCGGCCAAAC‑ATC‑ACCGACA‑CTGGGACCATCTGAACAACCAGAATT‑GGGGTG‑CCACGGGGCAAAACGAGATAGTCG‑TGGAAAAGCGTAATAAACCCTTCTTTCcgcg                                                                                                                            >  1:396769/1‑251 (MQ=255)
                                                                                                                        cATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCC‑TTTTTT‑ACGCCCC‑CG‑CGCGCGACCAAAC‑ATC‑ACCCACA‑CTTGCACCCACTGCACATCCAGATTT‑TGGTTG‑CTACGGTGCAAATCGAGATATTCG‑TGGGACAACTTAATAAACTCTTCTTTCTGCggg                                                                                                                          >  1:351807/1‑249 (MQ=255)
                                                                                                                           ccGCCATACGGCGCAGCGAAGCTGACGGCGAAAAACGCAAAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCC‑TTTTTC‑ACGCCCC‑GG‑CGCGCGACCAAAC‑ATC‑ACCGACA‑CTGGCACCATCTGCACATCCAGATTT‑GGGTTG‑CTACGGTGCAAATCGAGATAGTCG‑TGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTaa                                                                                                                       <  2:189008/251‑1 (MQ=255)
                                                                                                                             gCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACCTCGCC‑TTTTTT‑ACGCCCC‑CG‑CGCGCGGCCAAAA‑ATC‑ACCCACA‑CAGGCACCATCTTCACATCCCGATTT‑GGGGTG‑CTACGGGGCAAATCGAGATTGTCGGTGGA‑CAGCTTAATAGAATCTTCttttctcctgtcat                                                                                                                      >  2:135226/1‑239 (MQ=255)
                                                                                                                                 tACGGCGCAGAGAAACTGACGGAGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCAATTCGCC‑TTTTTC‑AAGCTCC‑GG‑TGAGCGACCAAAC‑ATCCACC‑ACA‑ATGGCACCATCTGCACCTCCAGAATT‑GGGGTG‑GTACGGGGCCAATCGAGATAAGCG‑TGGGAAAGATTAATAAAATCTTCTTTCGGTGGGTAAT‑AGGTg                                                                                                                >  1:351693/1‑251 (MQ=255)
                                                                                                                                  aCGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCC‑TTTTTT‑ACGCCCC‑CG‑CGCGCGGCCCAAC‑ATC‑ACCGACA‑CTGGCACCCTCTGCACATCCAGGTTT‑TGGTTG‑CTACGGGGCAAATCGAGATAATCG‑TGGAACAGATTAATATGCTCTTCTTTCTGCGTGTAAT‑aaggg                                                                                                                >  1:263044/1‑246 (MQ=255)
                                                                                                                                              aacagtcGGCGAAAAACGCACAAACGTGTCGAGCCCGAACCACAGTACAGCGAAAAATCTCTGTACGCCTTTGAGAGTACGCAGCGGCGGGTTAACTTCGCC‑TTTTTC‑ACGCCCC‑GG‑CGCGCGACCAAAC‑ACC‑ACCGACA‑CGGGCTCCATCTGCACATCCAGATTT‑GGGTTG‑CTACGGTGCAAATCGAGATAGTCG‑TGGAACAGCTTAATAGACTCGTCTTTCGTCGTGTAAT‑AGGTGAACACGCGCGGcc                                                                                                   <  2:145985/245‑1 (MQ=255)
                                                                                                                                              aaCTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCC‑TTTTTC‑ACGCCCC‑GG‑CGCGCGACCAAAC‑ATC‑ACCGACA‑CTGGCACCATCTGCACATCCAGATTT‑GGGTTG‑CTACGGTGCAAATCGAGATAGTCG‑TGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAAT‑AGGTGAACACACGCGGcc                                                                                                   <  1:570628/251‑1 (MQ=255)
                                                                                                                                                  gACGGCGAAAAACGCACAAAACGGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCC‑TTTTTC‑ACGCCCC‑GG‑CGCGCGACCAAAC‑ATC‑ACCGACA‑CTGGCACCATCTGCACATCCAGATTT‑GGGTTG‑CTACGGTGCAAATCGAGATAGTCG‑TGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAAT‑AGGTGAACACACGCGGCccgcc                                                                                               <  2:90135/251‑1 (MQ=255)
                                                                                                                                                        gagaAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCC‑TTTTTT‑ACGCCCC‑CG‑CGCGCGACCAAAC‑ATC‑ACCCACA‑CTGGCACCCTCTGCACATCCAGATTT‑TGGGTG‑CTACGGTGCAAATCGAGATAGTCG‑TGGAACAGCTTAATAGACTCTTCTTTCTGCGTGTAAT‑AGGGGAAAACACGCGGCccgccgg                                                                                             >  1:286871/1‑246 (MQ=255)
                                                                                                                                                                caaaaacctgccgcgACCGAGCCACAGTACAGCAAAAATTTTCTGTACGCCGTTAAGCATCCGCGGCGGCGGTTTCCCTTCGCC‑TTTTCC‑ACCCCCC‑GG‑CGCGCGACAAAAC‑ATC‑CCCGACA‑CTGGCACCATCTGCACATCCAGATTT‑GGGTTG‑CTACGGTGCAAATCGAGATATTCG‑TGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAAT‑AGGTGAACACACGCGGCCCGCCGTGAATGAACACAt                                                                                 <  1:237487/240‑1 (MQ=255)
                                                                                                                                                                      aCTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCTCC‑TTTTTT‑ACGCCCC‑CC‑CGCGCGACCAAAC‑ATC‑ACCCACA‑CTGGCACCATCTGCACCACCAGATTT‑TGGTTG‑CTACGGTGCCAATCGAGATAGTCG‑TGGGACAGCTTAATAAACTCTTCTTTCCGCGTGTAAA‑AGGTGAACACACGCCGCCCGCCGTGAATGAAcacaaaacgcg                                                                           >  1:219333/1‑244 (MQ=255)
                                                                                                                                                                                   aGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCCCTTCCCC‑TTTTTC‑ACGCCCC‑GG‑CGCGCGACCAAAC‑ATC‑ACCGACC‑CCGGCACCATCCGCACATCCAGATTT‑GGGGTG‑CTACGGGGCAAAACGAGATAGTCG‑CGGAACCGCTTAAAAGACTCTTTTTTCCGCGTGTAAA‑AGGTGAAAACACCCCGCCCGCCGCGAATGAAAACCCAGCGCCGCAGTAGCGGGcc                                                              >  1:247979/1‑251 (MQ=255)
                                                                                                                                                                                     ccACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCCCTTCGCC‑TTTTTC‑ACGCCCC‑GG‑CGCGCGACCAAAC‑ATC‑ACCGACA‑CTGGCACCATCTGCACATCCAGATTT‑GGGTTG‑CTACGGTGCAAATCGAGATAGTCG‑TGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAAT‑AGGTGAACACACGCGGCccgccg                                                                                              <  1:46761/217‑1 (MQ=255)
                                                                                                                                                                                                 gAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGATCACCTAGCC‑TTTTTT‑TCGCCCC‑CG‑CGCGCGACCAAAC‑ATC‑ACCCACA‑CTGGCACCACCTGACCAATCAGAATT‑GGGTTGACT‑CGGGGGAAAAAAAGATAGGCG‑TGGAACAGGTTAATAGAATCTTCATTCTGCGTGGAATGAGG‑GGACAAACGGCGCCCGCCGCGAATGAACACATAGCGCGGGAGGAGAGTGGCGGCGAGTTCCAGCg                                                >  1:247964/1‑251 (MQ=255)
                                                                                                                                                                                                         tcttgaCGCCGTTAAGCATACGCGGCGGCGGGT‑CCCTTCCCCCTTTCTC‑CCCCCCC‑GG‑CCCGCAACGAAAC‑ACC‑CCCGACA‑CTGGCACCATCTGCACATCCAGATTT‑GGGTTG‑CTACGGTACAAAGCGAGATAGTCG‑TGAAACAGCTTAATAGTCTCTTCTTTCGGCGTGTAAT‑AGGTAAACACTCGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTaa                                         <  1:195054/245‑1 (MQ=255)
                                                                                                                                                                                                                    ttAAGCATACGCAGCGGCGGGTTCACTTCGCC‑CTTTTT‑ACGCCCC‑GG‑CGCGCGACCCAAC‑ATC‑ACCGACA‑CTTGCACCATCTGCACATCCAGATTTTGGGTT‑‑CTACGGGGCAAATCGAGATAATCG‑TGGAACAGCTTAATAGAATCTTCTTTCGGCGTGTAAT‑AGGGGAACACACGCGGCCCCCCGTGAATTAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCCGCGGCTCTAAACGGTCAGGCa                              >  1:115881/1‑250 (MQ=255)
                                                                                                                                                                                                                      aaGCATGCGCGGGGGCGGGTTCATGTCGCC‑TTTTTC‑CCGCCCC‑GG‑CGCGCGACAAAAC‑ACC‑ACCGACA‑CGGGCACCATCTGCACATCAAGATTT‑GGGTTT‑CTACGGGGCAAATCGAGATAGTCG‑TGGAACAGCTTAATAGCCTCTTCTTTCGGCGTGTAAT‑AGGTGAACGCGCGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGt                           <  1:194310/251‑1 (MQ=255)
                                                                                                                                                                                                                       agcagcgccggcggcgGGTTCCCGTC‑CT‑TTTTTC‑CCCCCCC‑GGGCGCGCGACCAAAC‑ACC‑CCCGACT‑CGGGCCCCATTGGCACACCCAGTTTG‑GGGTTG‑CTACGGTGCAAATCGAGATAGTCG‑TGGAACAGCTAAATAGCCTTTTCTTGCGGCGTGTAAT‑GGGTGACCACCCGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTc                          <  2:76068/241‑1 (MQ=255)
                                                                                                                                                                                                                       aGCATCCGCGGCGGCGGGTCCACT‑CCCC‑TTTTTC‑CCCCCCCCGG‑CGCGCGACCAAAC‑ACC‑CCCGACA‑CTGGCACCATCTCCACATCCAGATTT‑GGGTTG‑CTACGGTGCAAATCGAGATAGTCG‑TGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAAT‑AGGTGACCACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTc                          <  1:23143/251‑1 (MQ=255)
                                                                                                                                                                                                                                ggcggcgGGTTCCTTTCCCC‑TTTTCC‑CCCCCCC‑GG‑CCCGCG‑CCAAACCATC‑CCCGACA‑CTGGCACCATCTGCACATCCAGATTT‑GGTTTG‑CTACGGTGCAAATCGAGATAGTCG‑TGGAACAGCTTAATAGACTCTTCTTTGGGCGTGTAAT‑GGGTGAACCCACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAg                 <  1:167623/250‑1 (MQ=255)
                                                                                                                                                                                                                                ggcggcgGGTTCACTCCGCC‑TTTTCC‑ACGCCCC‑GG‑CGCGCGCCAAAAC‑ATC‑CCCGACA‑CTGGCACCATCTGCACATCCAGATTT‑GGGTTG‑CTACGGTGCAAAGCGAGATAGTCG‑TGAAACAGCTTAATAGACTCTCCTTTCGGCGTGTAAT‑AGGGGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAg                 <  1:143908/250‑1 (MQ=255)
                                                                                                                                                                                                                                   ggcggGTTCACTTCGCC‑TTTTTC‑ACGCCCC‑GG‑CGCGCGACCAAAC‑ATC‑ACCGACA‑CTGGCACCATCTGCACATCCAGATTT‑GGGTTG‑CTACGGTGCAAATCGAGATAGTCG‑TGGAACAGCTTAATAGACTCTTCTTTCGGCGTGGAAT‑AGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCCGGCAAGTCATGTGCCAGacc              >  2:193402/1‑250 (MQ=255)
                                                                                                                                                                                                                                           cACTTCGCC‑TTTTTC‑ACGCCCC‑GG‑CGCGCGACCAAAC‑ATC‑ACCGACA‑CTGGCACCATCTGCACATCCAGATTT‑GGGTTG‑CTACGGTGCAAATCGAGATAGTCG‑TGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAAT‑AGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGAAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGgcgc      >  2:387709/1‑251 (MQ=255)
                                                                                                                                                                                                                                                cccc‑TTTTTCCACGCCCC‑GG‑CGCGCGACAAAAC‑ATC‑CCCGACA‑CTGGCACCATCTGCACATCCAGATTT‑GGTTTG‑CTACGGTGCAAATCGAGATAGTCG‑TGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAAT‑AGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCaa  <  1:49770/249‑1 (MQ=255)
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                          
ATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGAT‑AGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCC‑TTTTTC‑ACGCCCC‑GG‑CGCGCGACCAAAC‑ATC‑ACCGACA‑CTGGCACCATCTGCACATCCAGATTT‑GGGTTG‑CTACGGTGCAAATCGAGATAGTCG‑TGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAAT‑AGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAA  >  NC_000913/4255813‑4256301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑