Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,223,6440CT21.4% 170.7 / 17.1 70intergenic (‑16/‑184)iclR/metHDNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
Reads supporting (aligned to +/- strand):  ref base C (13/42);  new base T (14/1);  total (27/43)
Fisher's exact test for biased strand distribution p-value = 1.22e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGT  >  NC_000913/4223406‑4223893
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                            
ggcGCACGAAACCTGGCTGTTTCAGCGTGGTTAGCAGGCTGTGGGTCGTGGAATTGGGTAACCCCGCTTGTGGCGCCAGTACCGTGAGTGCCACTCTGCCATTGGATTCGGCAATCCGCTCCAGTAATTTCAGGCCACGCTGTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACTGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCGCTTCTTTCTGTATCGT‑GGaa                                                                                                                                                                                                                                                 <  2:205936/249‑1 (MQ=255)
    cACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGGTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAAtca                                                                                                                                                                                                                                             <  2:468784/251‑1 (MQ=255)
      cGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAAtca‑tt                                                                                                                                                                                                                                          <  1:551587/251‑1 (MQ=255)
      cGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAAtca‑tt                                                                                                                                                                                                                                          <  2:7659/251‑1 (MQ=255)
            ccTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAAtca‑ttttcatt                                                                                                                                                                                                                                    <  1:206509/251‑1 (MQ=255)
            ccTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAAtca‑ttttcatt                                                                                                                                                                                                                                    <  2:325416/251‑1 (MQ=255)
               gctgTGGCATCGGGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCTGCTTGTTGCGCCAGTTCAGTGAGGTCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACTTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCTT‑TTCG‑C‑GGGAATAGGTGC‑T‑AGCATGACAGTCTCTTGTGTTTGTATCGT‑GGAAATCA‑TTTTCAttttt                                                                                                                                                                                                                                 <  2:154219/251‑1 (MQ=255)
                  gTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGTCGCGT‑TTCG‑C‑GGGGAAGGGGGG‑G‑ACCATGACAGGCTCTATTTTTTGTAtggggggaagcatttttaattttttt                                                                                                                                                                                                                                >  1:112225/1‑226 (MQ=255)
                            ggTTAGCAGGCGGTGGGCCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AAt                                                                                                                                                                                                                   <  1:485124/251‑1 (MQ=255)
                                   aGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAg                                                                                                                                                                                                            <  1:428231/251‑1 (MQ=255)
                                   aGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTTCCGCGC‑TTCG‑CCGGGGA‑GGGGGC‑G‑GCCCCGACAAGCTCTTTTTTTTTTATCGT‑GGGAATCT‑TTTTTATTTTTTTTTTTAGCT‑AATGCCATa                                                                                                                                                                                                             >  2:318618/1‑250 (MQ=255)
                                        gTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCTGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATTACAGTCTCGTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTGATTGTTAGCT‑AATGCAATAGTTact                                                                                                                                                                                                       <  2:291752/251‑1 (MQ=255)
                                         ggggTCGTGGAATTGGTTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTTCCATTGGATTCGGCAATCCACGCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTACTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTtctg                                                                                                                                                                                                      <  1:258351/250‑1 (MQ=255)
                                           ggTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTGCCGCGG‑TTTG‑C‑GGGGATGGGGGG‑G‑ACCATGACAGGCTCTTTTTTTTGTATCGT‑GGGAATCA‑TTTTTATTTTTTTTTTTTGCT‑AATGAAATAgattctga                                                                                                                                                                                                     >  1:82515/1‑243 (MQ=255)
                                                      tGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGAt                                                                                                                                                                                          <  1:38935/250‑1 (MQ=255)
                                                       gggTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATg                                                                                                                                                                                         <  2:110451/250‑1 (MQ=255)
                                                         gTAACCCGGCTTGTTGCGCCAGTGCCGTGATTGCCACTCTTCCATTGGATTCGGCAATCCACGCGAGTAATTTCAGGCCACGCGTTAAAGTCTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTTC‑G‑ACCATGTCAGTCTCTTTTTTCTGTATCGT‑GCAAATCA‑TTTTCATTTTTATTTTTAGCT‑AAGGTAATAGTTACTGATCTGAGCCGATGAGt                                                                                                                                                                                      <  2:448816/251‑1 (MQ=255)
                                                            ccccGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑TCCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTaa                                                                                                                                                                                   <  2:500581/250‑1 (MQ=255)
                                                             cccGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTTCCGCGT‑TTTG‑C‑GGGGATGGGGGC‑GGACC‑GGGCAGGCTCTTTTTTTTGTATTGTTGGAAA‑CC‑TTTTTATTTTTTTTTTTTGCT‑AATGCTAAAATTACTTAACTGAttctgtgggttta                                                                                                                                                                                   >  1:427609/1‑238 (MQ=255)
                                                                 gCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTTTGCCGCGT‑TTCG‑C‑GGGGATGGGGGC‑G‑GCCATGACAGTCTCTTTTTTTTGTATCGG‑GGGAATAA‑TTTTCATTTTTTTTTTTATCT‑AATGGCATAGTTACTGAAATTATCCGATgtggtaatattg                                                                                                                                                                              >  1:283451/1‑240 (MQ=255)
                                                                    tgttgCGCCAGTTCCGCGAGTGACACACTGCCATTGCATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCAGGATTTTTGGCGGGT‑TTCGAC‑GGGA‑TGGGTGCTG‑CCCCAG‑CAGTGCCTTTTTTTTGTTTCGT‑GGAAATCAATTTTCCTTTTT‑TTGTTATCT‑AATGCCATAATTTCTGAAATTATCCgcttatttacaatttaac                                                                                                                                                                           >  2:224854/1‑234 (MQ=255)
                                                                        gcgcCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTTAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAAt                                                                                                                                                                       <  1:541705/251‑1 (MQ=255)
                                                                             atttCCGTGAGTACCACACTGCCATTGGATTCGGCAATCCACTCCAGGAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGTCAGTCTCTTTGTTCTGTATCGT‑GGAAATCT‑TTTTCCTTTTTATTGTTAGCT‑AAAGCAATAGTTACTGAACTGATCCGATGAGTGGATGTTGAACACAtctcct                                                                                                                                                                  <  2:502686/249‑3 (MQ=255)
                                                                             aGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCAt                                                                                                                                                                  <  1:229811/251‑1 (MQ=255)
                                                                               ttCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGGATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTTTGTATCGT‑GGGAATCA‑TTTTCATTTTTTTTTTTTGCT‑AATGCAATAATTACTGAAATGATCCGATGAGTTAATTTTTAAAAAATATCATGt                                                                                                                                                                >  1:195109/1‑251 (MQ=255)
                                                                                            acacTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTGCTGCGT‑TTCG‑C‑GGGGATGGGGGC‑G‑GCCATGCCAGTCTCTTTTTTTTGTATTGT‑GGGAATCC‑ATTTCTTTTTTTTTTTTTTTT‑AATGCAATAATTATTTTACTGCTCCCCTGAGTTTATGTTGTAAAAAACTCATGTTTCgtggggggcg                                                                                                                                                   >  2:355006/1‑245 (MQ=255)
                                                                                              acTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTGCCGCGT‑TTCG‑C‑GGGGATGGGGGC‑G‑ACCCTGACAGTCTCTTTTTTTTGTTTCGT‑GGGAATCA‑TTTTTATTTTTTTTTTTAGCT‑AATGCAATAGTTACTGAACTGATCCCAGGAGTTAATGTTGAACAAATCTCATGTTGCGGGGTGGTCGCt                                                                                                                                                 >  1:380082/1‑251 (MQ=255)
                                                                                                 gCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTTCCGCGT‑TTTG‑C‑GGGGATGGGGGG‑GGACC‑TGGCAGTTTCTTTTTTTTGTTTCGT‑GGAAATAA‑TTTTTATTTTTTTTGTTAGCT‑TATGCAATAGTTACTGAACTGATCCGATGAGATTATGTTGTACAAATATCATGTTTCgtggggggtgctttt                                                                                                                                              >  2:457351/1‑240 (MQ=255)
                                                                                                    aTTGGATTCGGCATTCCACTCCAGTAATTTCAGGCCACGCGTTAAAGGCTGAACCTGTCCAGTCGCTGTTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGATCAAATCTCATGTTGCGTGGTGGTCGCTTTTAcc                                                                                                                                           <  1:574996/251‑1 (MQ=255)
                                                                                                            cGGCAATCTGCTCCAGTAATTGCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑GTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGc                                                                                                                                   <  2:383682/251‑1 (MQ=255)
                                                                                                               cAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGtt                                                                                                                                <  1:435214/251‑1 (MQ=255)
                                                                                                               cAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGtt                                                                                                                                <  1:93682/251‑1 (MQ=255)
                                                                                                                aaTCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGttt                                                                                                                               <  2:195447/251‑1 (MQ=255)
                                                                                                                   ccACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTGTTCTGTATCGT‑GGAAATCA‑TTTTCAGTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTAt                                                                                                                             <  1:436295/250‑1 (MQ=255)
                                                                                                                          aGTAATGTCAGGCCACGCGTTCAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTTC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTAt                                                                                                                     <  2:195367/251‑1 (MQ=255)
                                                                                                                             aaTTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTTTGCCCCGC‑TTCG‑C‑GGGGATGGGGGC‑G‑ACCCTGACAGTCTCTTTTTTTTTTATCGT‑GGAAATCA‑TTTTTATTTTTTTTTTTTGCT‑AATGCAATAGTTACTGAACTGATCCGCTGAGTTTATGTTGTACAAAAATCATGTTGCGTGGTGGGCGCTTTTTCCACAGATGCGTTTTTGCCAGTATGGt                                                                                                                  >  1:559963/1‑251 (MQ=255)
                                                                                                                              aTTTCAGGCCAAGCGTTAAAGACTGAACCGGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTGTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGATTATGCCAGTATGGtt                                                                                                                 <  2:37108/251‑1 (MQ=255)
                                                                                                                                  cAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTttgtt                                                                                                             <  1:457351/251‑1 (MQ=255)
                                                                                                                                              tAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTttatt                                                                                                  <  1:241706/250‑1 (MQ=255)
                                                                                                                                                 aGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGTCTTCC‑C‑GGGATTGGG‑GG‑G‑GCCATGAAAGTCTCTTTTTTTTTTTTCGT‑GGAAATCA‑TTTTCTTTTTTTTTGTTAGCTTAAT‑CAATAGTTAATGAACACATTCCATGGGGTTATGTTTAACAAAACTTATGTTTCGCGGGGGTCGCTTTTTACACAGATGTGTTTATGACCGTGTGGGTTGTTTTATTTTTTTTaaa                                                                                               >  2:135221/1‑250 (MQ=255)
                                                                                                                                                      gAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTTCCGCGT‑TTCG‑C‑GGGGATGGGGGG‑G‑GCCATGACAGTCTCTTTTTTCTGTATCGT‑GGGAATCA‑TTTTTATTTTTTTTTTTAGCT‑TATGCAATAGTTACTGAACTGATCCGATGAGTTTATGTTGGACAAATCTCATTTTGCGTGGGGGTCGCTTTTTCCCCAGATGCGTTTTTGCCCGGTTGGTTTGTTTGATTTTTTTTTAATATggg                                                                                         >  1:454460/1‑251 (MQ=255)
                                                                                                                                                        aCCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGtt                                                                                       <  2:554320/251‑1 (MQ=255)
                                                                                                                                                          cTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGGATGGGGGC‑G‑ACCATGACAGTCTCTTTTTTTTGTATTGT‑GGGAAACA‑TTTTTATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAAAATAACCGATGGGTTAATGGTGAAAAAAACTCATTTTGCGTGGGGGTCGCTTTTATCACAGAAGCGTTTATGCCCGTATGGGTTGTTGAATTTTTATTAAATTTTGGTTgg                                                                                     >  1:99498/1‑250 (MQ=255)
                                                                                                                                                           tGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTTTGCCGCGT‑TTTG‑C‑GGGGATGGGGGC‑G‑GCCCAGACAGTCTCTTTTTTCTGTTTCGT‑GGGAATCA‑TTTTTCTTTTTTTTTTTTGCT‑AATGCAATAGTTACTGAACTGATCCGATGTGTTAATTTTTTAAAAATCTCATGt                                                                                                                                                                >  2:235537/1‑175 (MQ=255)
                                                                                                                                                           tGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGt                                                                                                                                                                <  1:235537/175‑1 (MQ=255)
                                                                                                                                                                aGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTGCCGCGG‑TTCG‑C‑GGGAATGGGGGC‑G‑GCCCTGACAGTCTCTTTTTTTTGTATCGT‑GGAAATCA‑TTTTTATTTTTTTTTTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTTATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTTCCACAGATGCGTTTATGCCCGTATGGTTTGTTGAATTTTTTTTTAATCTGGGTTGAGCgggt                                                                               >  1:32594/1‑248 (MQ=255)
                                                                                                                                                                 gTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAAt                                                                                                                                                                                                                                               <  2:177324/92‑1 (MQ=255)
                                                                                                                                                                       ggTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGa                                                                          <  1:304369/249‑1 (MQ=255)
                                                                                                                                                                          gCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCaa                                                                      <  1:331460/250‑1 (MQ=255)
                                                                                                                                                                          gCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAg                                                                     <  2:356975/251‑1 (MQ=255)
                                                                                                                                                                                       cggGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCaaa                                                        <  2:454460/251‑1 (MQ=255)
                                                                                                                                                                                          gTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTg                                                     >  2:1864/1‑251 (MQ=255)
                                                                                                                                                                                                              c‑gGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTgcgc                                     >  1:343663/1‑249 (MQ=255)
                                                                                                                                                                                                                 ggAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGtt                                 <  1:355006/251‑1 (MQ=255)
                                                                                                                                                                                                                 ggAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGtt                                 <  1:407480/251‑1 (MQ=255)
                                                                                                                                                                                                                   aaTGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCATTTaa                               <  2:492027/251‑1 (MQ=255)
                                                                                                                                                                                                                    aTGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATTCCAGTATGGTGTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTaaa                              <  1:462656/251‑1 (MQ=255)
                                                                                                                                                                                                                      gggTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATg                            >  1:77278/1‑251 (MQ=255)
                                                                                                                                                                                                                       ggTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGCACAACTGCGTGCGCAGTTAAATg                            >  2:559700/1‑250 (MQ=255)
                                                                                                                                                                                                                        gTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGa                           >  2:13367/1‑250 (MQ=255)
                                                                                                                                                                                                                             g‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGt                       >  1:558268/1‑250 (MQ=255)
                                                                                                                                                                                                                             g‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGt                       >  1:294680/1‑250 (MQ=255)
                                                                                                                                                                                                                               aCCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAACTCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGACAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCCAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTAt                     >  1:484258/1‑251 (MQ=255)
                                                                                                                                                                                                                                    gACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTgg                >  1:140916/1‑251 (MQ=255)
                                                                                                                                                                                                                                     aCAGTCTCTTTTTTCTGTATCGT‑GGAAAGCA‑TGTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGt               <  2:85313/251‑1 (MQ=255)
                                                                                                                                                                                                                                      cAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACGGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATTCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAGCTGCGTGCGCAGTTAAATGAACGTATTCTGGTg              <  2:159267/251‑1 (MQ=255)
                                                                                                                                                                                                                                            ctgttttCTGTATCGT‑GGAAAGCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCTCAGATGCGTGTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTgt          <  2:564653/246‑2 (MQ=255)
                                                                                                                                                                                                                                            ctTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTg           <  1:290005/248‑1 (MQ=255)
                                                                                                                                                                                                                                                  tCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGt  >  2:538223/1‑251 (MQ=255)
                                                                                                                                                                                                                                                  tCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGt  >  1:354610/1‑251 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                            
GACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGT‑TTCG‑C‑GGGAATGGGTGC‑G‑ACCATGACAGTCTCTTTTTTCTGTATCGT‑GGAAATCA‑TTTTCATTTTTATTGTTAGCT‑AATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGT  >  NC_000913/4223406‑4223893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑