Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,731,7130GA26.9% 90.1 / 11.6 53E195K (GAA→AAA) xylFxylose ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (24/14);  new base A (0/14);  total (24/29)
Fisher's exact test for biased strand distribution p-value = 2.38e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TTACGACCGTATGATTAACGATGCGGATATCGATTTTTATATTTCTTTCGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAA‑TTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGG‑TGACCAATGGGTT‑G‑ATGGCTGGT‑TACCGGAAAAC‑GCAT‑TGAAAAT‑TATGGAAAACGCGCTAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGT‑ATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAA  >  NC_000913/3731463‑3731958
                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                          
ttACGACCGTATGATTAACGATGCGGATATCGATTTTTATATTTCTTTCGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAA‑TTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGG‑TGACCAATGGGTT‑G‑ATGGCTGGT‑TACCgg                                                                                                                                                                                                                                                            >  1:428685/1‑251 (MQ=255)
          aTGATTAACGATGCGGATATCGATTTTTATATTTCTTTCGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAA‑TTAGTTCCTGAGGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGG‑TGACCAATGGGTT‑G‑ATGGCTGGT‑TACCGGAAAAC‑GCAt‑t                                                                                                                                                                                                                                                <  2:264313/251‑1 (MQ=255)
                   gATGCGGATATCGATTTTTATATTTCTTTCGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAA‑TTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGG‑TGACCAATGGGTT‑G‑ATGGCTGGT‑TACCGGAAAAC‑GCAT‑TGAAAAT‑TAt                                                                                                                                                                                                                                      >  1:417915/1‑251 (MQ=255)
                      gCGGATATCGATTTTTATATTTCTTTCGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAA‑TTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGG‑TGACCAATGGGTT‑G‑ATGGCTGGT‑TACCGGAAAAC‑GCAT‑TGAAAAT‑TATGGa                                                                                                                                                                                                                                   <  1:247899/251‑1 (MQ=255)
                         gcgatCGATTTTTATATTTCTTTCGATAACGAAAAAGTCGGTGAACTGCAGTCAAAAGCCCTGGTCGATATTGTTCCGCACGGTAA‑TTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCTCCGGACAAATGTAAGTGTTACAACCTTACGTTGATTCCGGCACAATTAAAGTCGTTGG‑TGACCAATGGGTT‑G‑ATGGCTGGT‑TACAGGAAAAC‑GCAT‑TGAAAAT‑TATGGaac                                                                                                                                                                                                                                 >  2:268717/4‑249 (MQ=255)
                          atatCGATTTTTATATTTCCTTCGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAA‑TTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGG‑TGACCAATGGGTT‑G‑ATGGCTGGT‑TACCGGAAAAC‑GCAT‑TGAAAAT‑TATGGaaaaa                                                                                                                                                                                                                               <  2:159418/251‑2 (MQ=255)
                                   tttATATTTCTTTCGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAA‑TTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGG‑TGACCAATGGGTT‑G‑ATGGCTGGT‑TACCGGAAAAC‑GCAT‑TGAAAAT‑TATGGAAAACGCGCTAA‑c                                                                                                                                                                                                                      <  1:367157/250‑1 (MQ=255)
                                        atTTCTTTCGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAA‑TTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGG‑TGACCAATGGGTT‑G‑ATGGCTGGT‑TACCGGAAAAC‑GCAT‑TGAAAAT‑TATGGAAAACGCGCTAA‑CCGCCaa                                                                                                                                                                                                                <  1:355520/251‑1 (MQ=255)
                                        atTTCTTTCGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAA‑TTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGG‑TGACCAATGGGTT‑G‑ATGGCTGGT‑TACCGGAAAAC‑GCAT‑TGAAAAT‑TATGGAAAACGCGCTAA‑CCGCCa                                                                                                                                                                                                                 <  2:361085/250‑1 (MQ=255)
                                                cGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAA‑TTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGG‑TGACCAATGGGTT‑G‑ATGGCTGGT‑TACCGGAAAAC‑GCAT‑TGAAAAT‑TATGGAAAACGCGCTAA‑CCGCCAATAATAAc                                                                                                                                                                                                         >  1:540116/1‑250 (MQ=255)
                                                            aGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATAGTTCCGCACGGTAA‑TTACTTCCGGATGGGCGGATCGACGGAAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTACGGAAAAATTAAAGTCGTTGG‑TGACCAAGGGGTG‑G‑ATGGCGGGT‑TACAGGAAAAC‑GCAT‑TGAAAAT‑TATGGAAAAGGAGATAA‑CGACAAATAATAACAAAATTGATGCTg                                                                                                                                                                                            >  2:477649/1‑251 (MQ=255)
                                                               gggTGAACGGAAGGAAAAACCCCTGGTGGATATTGTTCCGCA‑GGTAAATTCCTCCCTGTGGGGCGGCTCCCCGGTAGTCACCAACGCAAGGCTGTCCCGCGCGGGACAAATGAAAGTGTTAACACCTTGCGTTGATTCGGGAAAAATTAAAGTCGTTGT‑AGACCAATGGTTT‑G‑ATGGCGTGT‑TCCCGGAAAAC‑GCAT‑TAAAATT‑TATGGAAAACCCGCTAA‑CCGCCAATAATAACAAAATTGATGCTGTAg                                                                                                                                                                                         <  1:256107/250‑1 (MQ=255)
                                                                gggaaaCTGCAGCCAAAAGCCCTTGTCGATATTGTCCCGCAATGTAT‑TTACTTCCTGATGGGCGACGCCCCTGTAGATAACAACGCCAAGCTTTCCCGCGCCGAACAAATGAAAGTTTTAAACCCTTACGTGGAGTCCGAAAAAATTAAAGTTTTTGT‑GGACCAATGGGTT‑G‑ATGGCTGTT‑TCCCGAAAAAC‑GCAT‑TAAAATT‑TATGAAAAACGGGCTAG‑CCGCCAATAATAACAAAATTGATGCTGTAGt                                                                                                                                                                                        <  1:354891/247‑1 (MQ=255)
                                                                        gCAGGCAAAGGCCCTGGTCGATATTGTTCCGCAAGGTAA‑TTCCTTCCTGTTGGGCGGCTCGCGGGTAAATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAATTTTTAAAACCTTACGTTGATTCGGAAAAAATTAAATGCGTTGG‑TGACCAATGTGTT‑G‑ATGGCTGGT‑TCCCGAAAAAC‑GCAT‑TTAAAAT‑TAGGGAAAACTCGCTAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTc                                                                                                                                                                                  <  1:157852/249‑1 (MQ=255)
                                                                         cAGGCAAAAGCCCTGGACGATTTTGTTCCGCAAGGTAA‑TTACTACCTGATGGGCGGCTCGGCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAACTGAAAGTGTTCAAACATTACGTTGATTCCGGACAAATTAAAGTCGTTGG‑TGACCAATGGGTT‑G‑ATGGCTGGT‑TACCGGAAAAG‑GCAT‑TGAAAAT‑TATGGAAAACGCGCTAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCaa                                                                                                                                                                                >  2:432814/1‑250 (MQ=255)
                                                                          aGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAA‑TTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGG‑TGACCAATGGGTT‑G‑ATGGCTGGT‑TACCGGAAAAC‑GCAT‑TGAAAAT‑TATGGAAAACGCGCTAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAAc                                                                                                                                                                              >  2:27318/1‑251 (MQ=255)
                                                                           gcaaaaaGCCCTGGTGAATATTGTTCGGAAAGGTAT‑TTACTTCGTAGGGGGCGGTTCGCCGTTAGATAACAACGGCAAGCTGTTCCGCGCGGAACAAAGAAAAGTGTTAAAGCCTTATGTTGATTCCGGAAAAATTAAAGTCGTTGG‑GAACCAAGGGGTT‑G‑ATGGCTGGT‑TACCGAAAAAC‑GCAT‑ATAAAAT‑TAGGGAAACCGCGATAG‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACg                                                                                                                                                                             <  1:490265/248‑1 (MQ=255)
                                                                               aacccccTGATCGATATTGTCCCGCAAGGTAA‑TTACTCCCTGGTGGGCGTCTCGCGGGTAAAAAAAAACGCCAATCTGTTCCGCGGCGGACAAATGAAAGTGTTAAAACCTTACGTGTATCCCGAAAAAATTAAAGTCGTTGT‑TGAACAGTGGGTG‑G‑ATGGCTGGT‑TACCGAAAAAC‑GCAT‑TAAAAAT‑TATGGAAAACCCGCAAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGc                                                                                                                                                                         <  2:93805/247‑1 (MQ=255)
                                                                                 aGCCCTGGTCGATATTGTTCCGCAAGGTAA‑TTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGG‑TGACCAATGGGTT‑G‑ATGGCTGGT‑TACCGGAAAAC‑GCAT‑TGAAAAT‑TATGGAAAACGCGCTAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGcc                                                                                                                                                                        >  1:105125/1‑250 (MQ=255)
                                                                                                gttcccgaaaGGTAT‑TTCCTGCCTGATGGGGGGCGCGCCGGTAGAAAACAACGCCAAGCTGTCCCGCGAGGAAAAAATGAAAGTGTTAAATCCTTACGTTGTTTCGGAAAAAATTAAAGTCGTGGG‑TGACCAATGGGTT‑G‑ATGGCTGGT‑TACCGAAAAAC‑GCAT‑TGAAAAT‑TATGAAAAACGCGCTAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCaa                                                                                                                                                        <  2:13985/243‑1 (MQ=255)
                                                                                                 gTTCCGCAAGGTAA‑TTACTTCCTGATGGGCGGCACGCCGGTAGATAACACCGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAACGTCGTTGG‑TGACCAATGGGTT‑G‑ATGGCTGGT‑TACCGGAAAAC‑GCAT‑TGAAAAT‑TATGGAAAACGCGCTAA‑CCGCCAATAATAACAAAATTGATGCTGTCGTTGCCTCAAACGATGCCACCGCAGGTGGGTCAAt                                                                                                                                                       >  2:384930/1‑251 (MQ=255)
                                                                                                   cccgaaaGGTAA‑TTACTTCCGGAGGGGGGGCCCGCCGTTAGATAACAACGCCAAGTTGTCCCGCGCCGGACAAATGAAAGTGTTAAACCTTTACGTTGATTCCGAAAAAATTAAAGTCGTGGG‑TGCCCAATGGGTT‑G‑AGGGCTGGT‑TACCGAAAAAC‑GCAT‑GGAAAAT‑TATGGAAAACCCGATAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTc                                                                                                                                                     <  2:105125/246‑1 (MQ=255)
                                                                                                    tccgaaggtaaattgcatcctagtGGGCGGCTCGCCTGTAGATAACCACGCCAACCTGTCCGGCCCCAGACAAATGAATTTGTTAAATCCTTACGTTGGTTCGGAAAAAATTATAGTCTTTGT‑CGGCCAATGGGTT‑G‑CTGGCTTGT‑TACCGAAAAGC‑GCTG‑TAAAAAT‑TATGAAAGACCCGCAAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAAtt                                                                                                                                                      <  2:366108/227‑1 (MQ=255)
                                                                                                                        gggtGGGCGCCTCGCGGGTATAAAACACCGCCACGCTTTCCCGGGCCGAAAAAAGAAAATTGTAAAAACTTTACGTTGATTCCGAAAAAATAAAAGTCGTGGG‑TGACCAATGGGTT‑T‑AGGGGTGGT‑TCCCGAAAAAC‑GAAT‑GAAAAAT‑TATGGAAACCGCGCTAC‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGt                                                                                                                                 <  2:368021/248‑1 (MQ=255)
                                                                                                                               cggcTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGG‑TGACCAATGGGTT‑G‑ATGGCTGGT‑TACCGGAAAAC‑GCAT‑TGAAAAT‑TATGGAAAACGCGCTAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATc                                                                                                                            >  1:408325/1‑249 (MQ=255)
                                                                                                                                   gctccGGTAAAAAACACCGCCAAGCTGTTCCGAGCCGGAAAAATAAAATTGTAAAAACCTTCCGTTGATTCCGAAAAAATAAAAGTCGTTGT‑TGCCCAA‑GGGTT‑GGAGGGTTGGT‑TACCG‑AAAACTGCAT‑TAAAAAT‑TAGGAAAAACGCGCTAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGAGGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGaa                                                                                                                      <  2:438428/248‑1 (MQ=255)
                                                                                                                                   acgcccgtaaaaaacaccGCAAAGCTGTTCCGCGCCAGAAAAAGGAATGTGGTAAACCCTCACTTAGTTTCCGAAAAAATGAATGGCGTTGG‑TGCCCATGGGGTG‑G‑ATGGCGGGT‑CCCCGAAAAAC‑GCAT‑‑AAAAATGTATGAAAACCGCGCAAC‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGTAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGaa                                                                                                                      <  2:9816/239‑1 (MQ=255)
                                                                                                                                            gATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGG‑TGACCAATGGGTT‑G‑ATGGCTGGT‑TACCGGAAAAC‑GCAT‑TGAAAAT‑TATGGAAAACGCGCTAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAAt                                                                                                             >  2:17666/1‑251 (MQ=255)
                                                                                                                                                      ccAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTGACGTTGATTCCGGAAAAATTAAAGTCGTTGG‑TGACCAATGGGTT‑G‑ATGGCTGGT‑TACCGGAAAAC‑GCAT‑TGAAAAT‑TATGGAAAACGCGCTAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGACAGTAGCCATCTCCGGcc                                                                                                     >  2:339962/1‑249 (MQ=255)
                                                                                                                                                               cccGCGCGGGACAAATGAAAGTGATAAACCCTTGCGTGGATTACGAAAAAATTAAAGGCGTTGG‑TGACAGATGGGTG‑G‑ATGGCTGGT‑TACCGGAAAAC‑GCAT‑AGAAAAT‑TATGGAAAACGGGCTAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGAt                                                                                          <  2:355508/250‑1 (MQ=255)
                                                                                                                                                               cccGCGCCGAACAAATAAAAGTGTTAAAACCTTCCGTTGATTCCGAAAAAATAAAATTCGTTGG‑TTACCAATGGTTG‑G‑ATGGCTGGT‑TACCGGAAAAC‑ACTT‑TGAAAAT‑TAGGGAAAACGCGCGAT‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGTAATTCAGGCATTAAGCGCGCAATGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGa                                                                                           <  1:455340/249‑1 (MQ=255)
                                                                                                                                                                 cgcgcgtgaaaaaggAAAGTGTTAAAACTTTACGTGGATTCAGGAAAAAGTAAGTTGGTTGG‑TG‑CCAAAGGGTTTG‑ATGGC‑GGTGTACCGGAAAAC‑GCAT‑GAAAAAT‑TATGGAAAACGCGCTAC‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGAtct                                                                                        <  2:105390/237‑1 (MQ=255)
                                                                                                                                                                     ccGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGG‑TGACCAATGGGTT‑G‑ATGGCTGGT‑TACCGGAAAAC‑GCAT‑TGAAAAT‑TATGGAAAACGCGCTAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGACGCGGATCTCGc                                                                                     >  1:77447/1‑250 (MQ=255)
                                                                                                                                                                       ggACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGG‑TGACCAATGGGTT‑G‑ATGGCTGGT‑TACCGGAAAAC‑GCAT‑TGAAAAT‑TATGGAAAACGCGCTAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAgg                                                                                  >  1:357506/1‑251 (MQ=255)
                                                                                                                                                                       ggACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGG‑TGACCAATGGGTT‑G‑ATGGCTGGT‑TACCGGAAAAC‑GCAT‑TGAAAAT‑TATGGAAAACGCGCTAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGACACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAgg                                                                                  >  1:523639/1‑251 (MQ=255)
                                                                                                                                                                        gACAAATAAAAGTGTTAAACCCTTACGTTGATTCCGGAAAAATAAAAGTCGTGGG‑TGACCAATGGGTT‑G‑ATGGCTGGT‑TACCGAAAAAC‑GCAT‑TAAAAAT‑TATGGAAAACGCGCTAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGt                                                                                 <  1:27318/251‑1 (MQ=255)
                                                                                                                                                                         aaaaatgaaatgGTTAAACCCTTACGTTGATCCCGAAAAAATAAAAG‑CTTTGGGTGACAAATGGGTT‑G‑ATGGCGGTT‑TACCGAAAACC‑GCAT‑AAAAAAT‑TATGAAAAGCGCGCTAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTa                                                                                <  2:428685/249‑1 (MQ=255)
                                                                                                                                                                             aTGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGG‑TGACCACTGGGTT‑G‑ATGGCTGGT‑TACCGGAAAAC‑GCAT‑TGAAAAT‑TATGGAAAACGCGCTAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTa                                                                             >  2:21613/1‑250 (MQ=255)
                                                                                                                                                                                aaaGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGG‑TGACCAATGGGTT‑G‑ATGGCTGGT‑TACCGGAAAAC‑GCAT‑TGAAAAT‑TATGGCAAACGCGCTAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGAGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACg                                                                         >  2:1146/1‑251 (MQ=255)
                                                                                                                                                                                        aaaaCCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGG‑TGACCAATGGGTT‑G‑ATGGCTGGT‑TACCGGAAAAC‑GCAT‑TGAAAAT‑TATGGAAAACGCGCTAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTAGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATtgctg                                                                 >  2:54522/1‑251 (MQ=255)
                                                                                                                                                                                               tacttggATTCCGAAAAAATTAAAGTCGTGGG‑TGCCCAAGGGTTT‑G‑AGGGCTGTT‑AACCGAAAAAC‑GAAT‑AGAAAAT‑TAGGGAAAACGCGCAAC‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTa                                                           <  2:149588/244‑1 (MQ=255)
                                                                                                                                                                                                               aaataaaggTCGTGGG‑TGACAAAGGGGTT‑G‑AGGGCTGTT‑TACCGGAAAAC‑GCAT‑GAAAAAT‑TATGGAAACCGCGCTAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTAGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGgtg                                          <  1:54522/243‑1 (MQ=255)
                                                                                                                                                                                                                   tAAAGCCGTTGG‑TGCCCAATGGGTT‑G‑ATGGCTGGT‑TACCGGAAAAC‑GCAT‑TGAAAAT‑TATGGAAAACGCGATAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGt‑at                                      <  1:384755/250‑1 (MQ=255)
                                                                                                                                                                                                                       gTCGTTGG‑TGACCAATGGGTT‑G‑ATGGCTGGT‑TACCGGAAAAC‑GCAT‑TGAAAAT‑TATGGAAAACGCGCTAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGT‑ATAAAcc                                 >  1:573364/1‑251 (MQ=255)
                                                                                                                                                                                                                         gagtgggagacaaaTGGGTG‑G‑AGGGCGGGG‑TACCGAAAAAC‑ACAT‑TAAAAAT‑TATGGAAAACGCGATAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGT‑ATAAACCt                                <  2:77454/239‑1 (MQ=255)
                                                                                                                                                                                                                         cGTTGG‑TGACCAATGGGTT‑G‑ATGGCTGGT‑TACCGGAAAAC‑GCAT‑TGAAAAT‑TATGGAAAACGCGCTAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGT‑ATAAACCt                                >  2:322758/1‑250 (MQ=255)
                                                                                                                                                                                                                                   ccAATGGGTT‑G‑ATGGCTGGT‑TACCGGAAAAC‑GCAT‑TGAAAAT‑TATGGAAAACGCGCTAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGT‑ATAAACCTATTACgttgt                      >  1:514493/1‑251 (MQ=255)
                                                                                                                                                                                                                                     aaTGGGTT‑G‑TTGGCTGTT‑TACCGGAAAAC‑GCAT‑TGAAAAT‑TATGAAAACCGCGCTAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGT‑ATAAACCTATTACGttgttg                    <  1:21613/251‑1 (MQ=255)
                                                                                                                                                                                                                                      aTGGGTT‑G‑ATGGCTGGT‑TACCGGAAAAC‑GCAT‑TGAAAAT‑TATGGAAAACGCGCTAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGT‑ATAAACCTATTACGTTGTTgg                   <  1:17666/251‑1 (MQ=255)
                                                                                                                                                                                                                                         ggTT‑G‑ATGGCTGGT‑TACCGGAAAAC‑GCAT‑TGAAAAT‑TATGGAAAACGCGCTAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGT‑ATAAACCTATTACGTTGTTGGCa                 >  1:121230/1‑250 (MQ=255)
                                                                                                                                                                                                                                              gaatggagggT‑TACCGAAGAAC‑TCAT‑TGAAAAT‑TATGTAGAACGCGCTAC‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGT‑ATAAGCCTATTACGTTGTTGGCAAAt              <  2:244284/242‑1 (MQ=255)
                                                                                                                                                                                                                                              g‑ATGGCTGGT‑TACCGGAAAAC‑GCAT‑TGAAAAT‑TATGGAAAACGCGCTAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGT‑ATAAACCTATTACGTTGTTGGCAAATAc            >  1:77123/1‑251 (MQ=255)
                                                                                                                                                                                                                                                    cTGGT‑TACCGGAAAAC‑GCAT‑TGAAAAT‑TATGGAAAACGCGCTAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGT‑ATAAACCTATTACGTTGTTGGCAAATACTGCCg       >  2:386263/1‑251 (MQ=255)
                                                                                                                                                                                                                                                       gT‑TACCGGAAATC‑GCAT‑TGAAGAT‑TCTGGAAAACGCGCTAA‑CTGCCAATAATAACAAATTTGATGATGTAGTTGACTCAAACGTTCCCACCGCAGGTGGCGCAATTCTGGCATTAAGCGCGCCAGGTTTATCAGGGTAAGTAGCAATCTCCGACCAGGATGCGGATCTCGCAGGTATTAAACGTATTTCTGCCGGTCCGCAAATTATGACGG‑GTCATACACCTCTTACTTTGTTTGCATATACTGCCGc      >  2:26960/1‑249 (MQ=255)
                                                                                                                                                                                                                                                           agctgAAAAC‑ACATGTGAAAAT‑TATGGAAA‑CGGCCTAATCCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGT‑ATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGaa  <  2:237469/247‑1 (MQ=255)
                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                          
TTACGACCGTATGATTAACGATGCGGATATCGATTTTTATATTTCTTTCGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAA‑TTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGG‑TGACCAATGGGTT‑G‑ATGGCTGGT‑TACCGGAAAAC‑GCAT‑TGAAAAT‑TATGGAAAACGCGCTAA‑CCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGT‑ATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAA  >  NC_000913/3731463‑3731958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑