Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	4,349,338	IS1 (+) +10 bp	coding (688‑697/720 nt)	dcuR ←	DNA‑binding transcriptional activator DcuR

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1978503 =	NA (NA)	100 (1.460)	87/492	0.0	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	4349338 =	0 (0.000)					coding (697/720 nt)	dcuR	DNA‑binding transcriptional activator DcuR
*	?	NC_000913	3583428 =	NA (NA)	48 (0.700)	45/492	0.4	100%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	= 4349347	0 (0.000)					coding (688/720 nt)	dcuR	DNA‑binding transcriptional activator DcuR

GGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAGGGTGAC  >  NC_000913/1978502‑1978752  |                                                                                                                                                                                                                                                          gggTAAGGGCTCCAACTTATTGATAGGGTTTTAGGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGGGAACGACAGCGACTTCCGGCCCAGCCGGGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTGCGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAGGGTGAc  <  2:127192/251‑1 (MQ=11)  |                                                                                                                                                                                                                                                          GGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAGGGTGAC  >  NC_000913/1978502‑1978752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.