Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,640,045	+C	coding (13/534 nt)	rrrQ ←	putative lysozyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,640,045	1	.	C	100.0%	223.8 / NA	59	I5V (ATC→GTC)	rrrQ	putative lysozyme
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (32/27);  total (32/27)

ATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGAT‑TTTTGTGTTCATAGATATTTATGATGAGGACGCTCGTGCTTATTGGCAGGATTTTCAATCTTAAAGGAGTACTGATGCTGCAGATAAGACTCAACTTTTTCTGACAATTTTTCTGCTACTTCCAGGAAGACTTGCCGGACGCTCCTTCTGGCTGCTGCCTCATAAAACTCCAGCGCAGCTCCTTCAACACGGTCCATGGCGACATCCAGGTCAAAAATTTCACCGTCAAAGCGTT  >  NC_000913/1639796‑1640280                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                            aTGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGAtc                                                                                                                                                                                                                                             >  2:377668/1‑250 (MQ=255)         gcCTTATCACGCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTgtgt                                                                                                                                                                                                                                     >  2:28999/1‑251 (MQ=255)          ccTTATCACGCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCGCCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGtt                                                                                                                                                                                                                                    <  2:526983/251‑1 (MQ=255)          ccTTATCACGCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGtt                                                                                                                                                                                                                                    >  1:169588/1‑251 (MQ=255)             tATCACGATCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTGTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGCTCTTTTGTGTTCa                                                                                                                                                                                                                                  <  2:273465/250‑1 (MQ=255)              aTCACGCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcc                                                                                                                                                                                                                                <  1:290040‑M1/251‑3 (MQ=255)                      cAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgct                                                                                                                                                                                                                        <  2:164702‑M1/251‑11 (MQ=255)                      cAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccg                                                                                                                                                                                                                          <  2:229934‑M1/249‑9 (MQ=255)                        aTGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttc                                                                                                                                                                                                                      <  1:294127‑M1/251‑13 (MQ=255)                            cgttgcccttgccgcatttttccttCGCCAGTTTCAGATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCGTCTTTTTCGTCCAGAACCTGGTCAAGGATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTGGTGTTCTcccgcccgcttcgtct                                                                                                                                                                                                                  <  2:320718‑M1/239‑17 (MQ=255)                                gACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttac                                                                                                                                                                                                               <  1:106534‑M1/250‑20 (MQ=255)                                                     cGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttggg                                                                                                                                                                                         >  1:6114‑M1/1‑210 (MQ=255)                                                          gTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcat                                                                                                                                                                                    >  2:365819‑M1/1‑205 (MQ=255)                                                           tttCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcat                                                                                                                                                                                    >  1:143413‑M1/1‑204 (MQ=255)                                                             tCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatact                                                                                                                                                                                 >  1:91593‑M1/1‑202 (MQ=255)                                                                tatTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgttattcatcatacactttgggatcatactga                                                                                                                                                                               >  2:442585‑M1/1‑199 (MQ=255)                                                                aattGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaa                                                                                                                                                                              <  1:221528‑M1/250‑53 (MQ=255)                                                                 aatggGCAAAAGGGTTTTGCCATCCACCACTGTGGCACCCCGACAGATGGTCCGTATGCCAGAACCATCGCGGTATGCCATTGAGAGGTTACCTTCTTTTTCGTCCAGCAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGAACCAATCAGCGCCAGAACGGCAACCGACCGGCCGTATCAGATCTTTTGTGTTCAcccgcccgcttcgtattacgaaattcatcataaacttagggatcatactccac                                                                                                                                                                             >  2:94158‑M1/3‑198 (MQ=255)                                                                       aaaaaCGGTTTTTCCATCCACCACTGTGGCACCCCGCCAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCCGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgccttacgatattcatcataaactttgggatcatactgaagctcccc                                                                                                                                                                       >  2:379181‑M1/1‑192 (MQ=255)                                                                       aaaaaCGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctcccc                                                                                                                                                                       <  1:28975‑M1/251‑60 (MQ=255)                                                                       aaaaaCGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccacccgcttcttcttacgatattcatcataaactttgggatcatactgaagctcccc                                                                                                                                                                       >  2:117928‑M1/1‑192 (MQ=255)                                                                        aaaaCGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgctt                                                                                                                                                                                                                       <  1:526769‑M1/202‑12 (MQ=255)                                                                        aaaaCGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgctt                                                                                                                                                                                                                       >  2:526769‑M1/1‑191 (MQ=255)                                                                                     catccaCCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcct                                                                                                                                                         >  1:105757‑M1/1‑178 (MQ=255)                                                                                        ccaccaCTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataacctttgggatcatactgcagctcccctccagatgcctcctgt                                                                                                                                                       >  2:46743‑M1/1‑175 (MQ=255)                                                                                           ccacTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGCCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagac                                                                                                                                                   <  1:106731‑M1/251‑80 (MQ=255)                                                                                                    cACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGCATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgacgctccccgccagatgcctcctttagaacccacgcac                                                                                                                                          >  2:181264‑M1/1‑163 (MQ=255)                                                                                                            cAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcg                                                                                                                                  >  2:127988‑M1/1‑155 (MQ=255)                                                                                                              gATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcggg                                                                                                                                >  2:299095‑M1/1‑153 (MQ=255)                                                                                                                      atatGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaat                                                                                                                        <  1:510900‑M1/251‑107 (MQ=255)                                                                                                                         tGCCAGAACCATCGCGGGATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatccc                                                                                                                     >  1:118356‑M1/1‑142 (MQ=255)                                                                                                                             aGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCAACAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaaatttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccattt                                                                                                                 >  2:542711‑M1/1‑138 (MQ=255)                                                                                                                             aGAACCATCGCGGTATGCCAGTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatccccttt                                                                                                                 <  2:149058‑M1/251‑114 (MQ=255)                                                                                                                               aaCCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttcc                                                                                                               >  1:550289‑M1/1‑136 (MQ=255)                                                                                                                                  cATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagc                                                                                                            <  2:555623‑M1/251‑119 (MQ=255)                                                                                                                                   aTCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagct                                                                                                           >  1:281755‑M1/1‑132 (MQ=255)                                                                                                                                           aTGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgcctgatgcctcctgtagacgcatcgcgcgcccttcgggaactaaatcccctttccagctataaagcg                                                                                                   >  2:49929‑M1/1‑124 (MQ=255)                                                                                                                                            tGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggcactaaatcccctttccagctataaagcg                                                                                                   >  2:304663‑M1/1‑123 (MQ=255)                                                                                                                                             gCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcga                                                                                                  >  1:532866‑M1/1‑122 (MQ=255)                                                                                                                                                                  gttgcgtcaAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgct                                                                               <  2:553816‑M1/244‑148 (MQ=255)                                                                                                                                                                      cGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtg                                                                        <  1:377668‑M1/251‑155 (MQ=255)                                                                                                                                                                       gTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgc                                                                       <  2:146481‑M1/251‑156 (MQ=255)                                                                                                                                                                         ccAGAAACTGGTCAAGTATCTGAGGGGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagatataaagcgaagccaaacgaataactgctgcttgtgca                                                                      >  2:121829‑M1/1‑94 (MQ=255)                                                                                                                                                                         ccAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgca                                                                      <  1:127988‑M1/250‑157 (MQ=255)                                                                                                                                                                              aaCTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgcctgatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagtttt                                                                <  1:49929‑M1/251‑163 (MQ=255)                                                                                                                                                                                cTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgt                                                              >  1:373542‑M1/1‑87 (MQ=255)                                                                                                                                                                                cTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgcagcttgtgcaagttttgt                                                              >  2:280486‑M1/1‑87 (MQ=255)                                                                                                                                                                                     cAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttg                                                         <  1:365819‑M1/251‑170 (MQ=255)                                                                                                                                                                                      aaGTATCTGAGGGGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttga                                                        <  2:169588‑M1/251‑171 (MQ=255)                                                                                                                                                                                      aaGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttg                                                         >  2:29594‑M1/1‑81 (MQ=255)                                                                                                                                                                                         tATCTGAGGAGCAGATGAGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggataatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagatttgtttttgaacc                                                     >  1:454986‑M1/1‑78 (MQ=255)                                                                                                                                                                                                             cAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgaaattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgacccgaaatacaaaagagcgtc                                   >  1:500378‑M1/1‑58 (MQ=255)                                                                                                                                                                                                                 cccaATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatacaaaagagcgtcagtttt                             <  2:93381‑M1/250‑198 (MQ=255)                                                                                                                                                                                                                    aaTCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatacaaaagagcgtcagtttta                            >  1:230802‑M1/1‑51 (MQ=255)                                                                                                                                                                                                                       catcgccagAACGGCTGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaatcgaaatacaaaagagcgtcaggtttaagcat                       <  1:287689‑M1/248‑204 (MQ=255)                                                                                                                                                                                                                           gcccgaacgGCAGCCGTCTGGCTGTATCTGATCTTATGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactcaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaagacaaaagagcgtcagttttaagcatttaa                   <  2:347660‑M1/247‑208 (MQ=255)                                                                                                                                                                                                                                aaCGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatacttaagctcccccccagatgcctcctgtagacgcatcgagcgaccttcgggaactaaatcccatttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaacgaaatacaaaagagcgtcagttttaagcatttaaaaca               >  2:293004‑M1/1‑39 (MQ=255)                                                                                                                                                                                                                                 aCGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatacaaaagagcgtcagtgttaagcatttaaaacacc             >  2:68248‑M1/1‑38 (MQ=255)                                                                                                                                                                                                                                     cAGCCGACAGGCCGTTTCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatacaaaagagcgtcagttttaagcatttaaaacaccttta         <  2:491567‑M1/251‑218 (MQ=255)                                                                                                                                                                                                                                            cAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatacaaaagagcgtcagttttaagcatttaaaacacctttattgttag  <  2:105757‑M1/251‑225 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                            ATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGAT‑TTTTGTGTTCATAGATATTTATGATGAGGACGCTCGTGCTTATTGGCAGGATTTTCAATCTTAAAGGAGTACTGATGCTGCAGATAAGACTCAACTTTTTCTGACAATTTTTCTGCTACTTCCAGGAAGACTTGCCGGACGCTCCTTCTGGCTGCTGCCTCATAAAACTCCAGCGCAGCTCCTTCAACACGGTCCATGGCGACATCCAGGTCAAAAATTTCACCGTCAAAGCGTT  >  NC_000913/1639796‑1640280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑