Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,867,576	G→T	intergenic (‑154/‑152)	ibpA ← / → yidQ	small heat shock protein IbpA/DUF1375 domain‑containing putative lipoprotein YidQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,867,576	0	G	T	100.0%	294.2 / NA	73	intergenic (‑154/‑152)	ibpA/yidQ	small heat shock protein IbpA/DUF1375 domain‑containing putative lipoprotein YidQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (42/31);  total (42/31)

ACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGCAGTTTATGGTCTATTGCATAGACTGAGGGGGCAGCACGAAACGTTAAAGTGCGATAGCCGCCACAGTGCGACGTAATGATGATGTTATTTTCAGCAAGGGATCGCTATAACTCATCAGGCCAACACCCACGATCAACAGATGAATTTATTATGATAAGAAATGTGTTGTTAGCGTTCATGATATGCAGCGGAATGACATTACTCGGAGGATGCTCCAGCGTGATGTCCCATACCGGA  >  NC_000913/3867333‑3867814                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               cctctggctctggTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGtttt                                                                                                                                                                                                                                         >  2:819122/2‑250 (MQ=255)    ctggctctggTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATgg                                                                                                                                                                                                                                      >  1:982814/1‑251 (MQ=255)     tggctctggTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGt                                                                                                                                                                                                                                     >  1:68258/1‑251 (MQ=255)     tggctctggTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGt                                                                                                                                                                                                                                     >  1:309850/1‑251 (MQ=255)       gctctggTTGTTTTCTAAGTGGTTAAACAAACGGTCAAAACCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCt                                                                                                                                                                                                                                   <  2:271812/251‑1 (MQ=255)         tctggTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTAt                                                                                                                                                                                                                                 >  2:1164219/1‑251 (MQ=255)          cggttggttTTCTAAGGGGTAAAACAACCGGTCAAACCCAATACCAGAACGGTAAAGCGGGAATAAATAAAAGTTACGAATAACCAATAGCTC‑TGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTAt                                                                                                                                                                                                                                 <  1:543621/243‑1 (MQ=255)                gttTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAg                                                                                                                                                                                                                          <  1:268179/251‑1 (MQ=255)                 ttttCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAGATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAg                                                                                                                                                                                                                          <  1:255966/250‑1 (MQ=255)                   ttCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGta                                                                                                                                                                                                                        <  2:791531‑M1/250‑3 (MQ=255)                    tCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtag                                                                                                                                                                                                                       <  1:1112108‑M1/250‑4 (MQ=255)                     cTAAGTGGTTAAACAAAAGGTAAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACATTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGt                                                                                                                                                                                                                         >  2:944669‑M1/1‑246 (MQ=255)                       aaGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagattt                                                                                                                                                                                                                   <  1:607381‑M1/251‑8 (MQ=255)                       aaGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagat                                                                                                                                                                                                                     <  2:189658‑M1/249‑6 (MQ=255)                                aacaaacGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTAGTGCATAGtagatttgcgggat                                                                                                                                                                                                            <  2:101616‑M1/249‑15 (MQ=255)                                aacaaacGGTAAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggtt                                                                                                                                                                                                            >  1:101616‑M1/1‑235 (MQ=255)                                 acaaacGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCGCCTGAAATCAGCGAGAATGTAAGTCCTTCCACAATGGACAGGTCAGGTAGCCAGAACTCCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgat                                                                                                                                                                                                         <  1:453400‑M1/251‑18 (MQ=255)                                   aaacGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgata                                                                                                                                                                                                        >  1:34127‑M1/1‑232 (MQ=255)                                    aacGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAATACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataat                                                                                                                                                                                                      <  1:634348‑M1/251‑21 (MQ=255)                                    aacGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAACAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataat                                                                                                                                                                                                      >  2:1064647‑M1/1‑231 (MQ=255)                                    aacGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataat                                                                                                                                                                                                      >  1:1143268‑M1/1‑231 (MQ=255)                                      cGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCTAGAATGTAAGCCCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGGCTATTGCATAGtagatttgcgggatgataattg                                                                                                                                                                                                    <  2:353081‑M1/251‑23 (MQ=255)                                           aaaTCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggat                                                                                                                                                                                               >  1:354769‑M1/1‑224 (MQ=255)                                           aaaTCCAAAAGCAGAACGGTAAAGCGGGGATAAATAAAAGTGACGAATAACCAAGAGCTGCTGAAATCAGCGAGAATGTAAGCCCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCTTCCTCAACGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTAAAGCATTATCAGCGGACTTTTTTTTTCAGTGTATGGTCTATTGCATAGtagatttgcgggatgataattgcggat                                                                                                                                                                                               <  2:77299‑M1/251‑28 (MQ=255)                                               ccAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgc                                                                                                                                                                                            <  1:272260‑M1/250‑31 (MQ=255)                                                    aGCAGAAAGGTAAAGAGGTGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGAAAGACCTTCCACACTGAACAGGTCAAGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCAACGATGTAAAAATGGGTCTGGAAATGAACTTTTAAAGCCTTAGCAGCGTACTTTTTTTTTCAGTTTATGGTCTATTGCAAAGtagatttgcggtgtgataattgcggattgtagaaat                                                                                                                                                                                      >  2:714401‑M1/1‑215 (MQ=255)                                                       aGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgtgatgataattacggattgcagaaataaa                                                                                                                                                                                   <  1:246845‑M1/251‑40 (MQ=255)                                                       aGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgg                                                                                                                                                                                                               <  2:207275‑M1/223‑12 (MQ=255)                                                       aGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgg                                                                                                                                                                                                               >  1:207275‑M1/1‑212 (MQ=255)                                                             gTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggc                                                                                                                                                                             >  1:828235‑M1/1‑206 (MQ=255)                                                               aaaGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcg                                                                                                                                                                            <  2:354769‑M1/250‑47 (MQ=255)                                                                aaGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggctgataattgcggattgcaggaataaaaagggcggg                                                                                                                                                                          >  2:819629‑M1/1‑203 (MQ=255)                                                                aaGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcgg                                                                                                                                                                           >  1:108767‑M1/1‑203 (MQ=255)                                                                 aGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCCTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcgg                                                                                                                                                                           >  2:576913‑M1/1‑202 (MQ=255)                                                                   cGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcgg                                                                                                                                                                           >  2:699907‑M1/1‑200 (MQ=255)                                                                          aaaTCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctcc                                                                                                                                                                <  2:905913‑M1/251‑59 (MQ=255)                                                                                aaGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgccctt                                                                                                                                                          >  1:482970‑M1/1‑187 (MQ=255)                                                                                                  aGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgcc                                                                                                                                        >  1:369544‑M1/1‑169 (MQ=255)                                                                                                  aGCTCCTGAAAACAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgcc                                                                                                                                        <  2:1106775‑M1/251‑83 (MQ=255)                                                                                                       cTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatg                                                                                                                                   >  2:129167‑M1/1‑164 (MQ=255)                                                                                                          aaaTCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCCGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcgg                                                                                                                                >  1:637784‑M1/1‑161 (MQ=255)                                                                                                                           aGACCTTACACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGCACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCCTAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaaacccttatc                                                                                                                 >  2:358362‑M1/1‑144 (MQ=255)                                                                                                                            gACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagctt                                                                                                                                              <  1:974764‑M1/219‑77 (MQ=255)                                                                                                                            gACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagctt                                                                                                                                              >  2:974764‑M1/1‑143 (MQ=255)                                                                                                                                      aaTGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatcccgcctacaaaat                                                                                                    >  2:1143094‑M1/1‑133 (MQ=255)                                                                                                                                           aCAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggctgcggcgtgaacgccttatccggcctacaaaatcatg                                                                                                >  2:610723‑M1/1‑128 (MQ=255)                                                                                                                                             aggtcaggtAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaa                                                                                              >  2:488691‑M1/1‑126 (MQ=255)                                                                                                                                                  aggtAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattc                                                                                         >  1:372479‑M1/1‑121 (MQ=255)                                                                                                                                                       gCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatac                                                                                    >  2:564481‑M1/1‑116 (MQ=255)                                                                                                                                                             acacCCATCAGTCGCGTCATCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccgtatccggcctacaaaatcatgaaaatgcaatacattgcaa                                                                             <  2:998639‑M1/251‑142 (MQ=255)                                                                                                                                                              cacCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaag                                                                            <  1:564481‑M1/251‑143 (MQ=255)                                                                                                                                                                cccATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagat                                                                          <  2:34127‑M1/251‑145 (MQ=255)                                                                                                                                                                  cATCAGGCGCGCCCTCATCGGCTACGATGTAGAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGTGCTTTTTTTTTCAGGTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagattatctccgcccttctctgatagctacttgacggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaggattt                                                                        <  2:982814‑M1/251‑147 (MQ=255)                                                                                                                                                                     cAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcg                                                                     <  1:1164219‑M1/251‑150 (MQ=255)                                                                                                                                                                      aGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgt                                                                    <  1:614792‑M1/251‑151 (MQ=255)                                                                                                                                                                      aGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCCTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgt                                                                    <  1:576913‑M1/251‑151 (MQ=255)                                                                                                                                                                              ccTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcctg                                                             >  1:267036‑M1/1‑93 (MQ=255)                                                                                                                                                                                      gCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcctgataagcgt                                                     >  1:252063‑M1/1‑85 (MQ=255)                                                                                                                                                                                         aCGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcctgataagcgtagcg                                                 <  1:690005‑M1/251‑170 (MQ=255)                                                                                                                                                                                                      gggTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcctgataagcgtagcgcatcaggcacgct                                    >  1:625161‑M1/1‑69 (MQ=255)                                                                                                                                                                                                          cTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcct                                                                                                           >  1:142222‑M1/1‑65 (MQ=255)                                                                                                                                                                                                          cTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcct                                                                                                           <  2:142222‑M1/176‑112 (MQ=255)                                                                                                                                                                                                            ggAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgcacgccttatccggcctccaaaatcatgaaaattcaatacattgcaagattttcgtaggcctgataagcctagcgcatcaggcacgctcgcatg                              >  1:1093931‑M1/1‑63 (MQ=255)                                                                                                                                                                                                             gAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcctgataagcgtagcgcatcaggcacgctcgcatgg                             <  2:267036‑M1/251‑190 (MQ=255)                                                                                                                                                                                                               aaTGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcctgataagcgtagcgcatcaggcacgctcgcatggtt                           >  1:320098‑M1/1‑60 (MQ=255)                                                                                                                                                                                                                aTGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATTGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagatgttcgtaggcctgataagcgtagcgcatcaggcacgctcgcatggtta                          <  1:922928‑M1/251‑193 (MQ=255)                                                                                                                                                                                                                      ttttCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcctgataagcgtagcgcatcaggcacgctcgcatggttagcgccc                    >  1:22961‑M1/1‑53 (MQ=255)                                                                                                                                                                                                                       tttCAAGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctaaaaaatcatgaaaattcaatacattgcaagattttcgtaggcctgataagcgtagcgcatcaagcacgctcgcctggttagcgccct                   >  2:1075931‑M1/1‑52 (MQ=255)                                                                                                                                                                                                                            aGCCTTATCAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGcagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacaccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtacgcctgataagcgtagcgcatcaggcacgctcgcatggttagcgccattaaat              >  1:415467‑M1/1‑47 (MQ=255)                                                                                                                                                                                                                                ttATCCGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggccttcaaaatcatgaaaattctatacattgcaagattttcgtaggcctgataagggtagcgcatcaggcacgctcgcatggttagcgccattaaatat            >  1:24826‑M1/1‑43 (MQ=255)                                                                                                                                                                                                                                    cAGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcctgataagcgtagcgcatcaggcacgctcgcatggttagcgccattaaatatcgatat      >  2:365243‑M1/1‑39 (MQ=255)                                                                                                                                                                                                                                     aGCGGACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaacttcaatacattgcaagattttcggaggcctgataagcgaagcgcatcaggcacgcccgcatggttagcgccattaaatatcgatatt     >  1:384890‑M1/1‑38 (MQ=255)                                                                                                                                                                                                                                        ggACTTTTTTTTTCAGTTTATGGTCTATTGCATAGtagatttgcgggatgataattgcggattgcagaaataaaaagggcggagatgatctccgcccttttcttatagcttcttgccggatgcggcgtgaacgccttatccggcctacaaaatcatgaaaattcaatacattgcaagattttcgtaggcctgataagcgtagcgcatcaggcacgctcgcatggttagcgccattaaatatcgatattcgc  >  1:861027‑M1/1‑35 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               ACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGCAGTTTATGGTCTATTGCATAGACTGAGGGGGCAGCACGAAACGTTAAAGTGCGATAGCCGCCACAGTGCGACGTAATGATGATGTTATTTTCAGCAAGGGATCGCTATAACTCATCAGGCCAACACCCACGATCAACAGATGAATTTATTATGATAAGAAATGTGTTGTTAGCGTTCATGATATGCAGCGGAATGACATTACTCGGAGGATGCTCCAGCGTGATGTCCCATACCGGA  >  NC_000913/3867333‑3867814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑