Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,652,331	T→C	intergenic (+2346/+397)	ydfD → / ← ynfP	lysis protein/protein YnfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,652,331	0	T	C	98.9%	344.2 / NA	94	intergenic (+2346/+397)	ydfD/ynfP	lysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (47/45);  total (49/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACTTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCAT  >  NC_000913/1652094‑1652573                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      gtTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATg                                                                                                                                                                                                                                          <  2:224355/250‑1 (MQ=255) gtTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAA‑CGGGTCATTAATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTTAACAAAGAGCCTCTCATTTATGTT‑TACCTC‑agagcatatgg                                                                                                                                                                                                                                         >  2:928691/1‑244 (MQ=255)  tttCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTTATGTT‑CACCTC‑AGAGAGTATGGc                                                                                                                                                                                                                                        >  1:416051/1‑251 (MQ=255)   ttCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGc                                                                                                                                                                                                                                        <  2:608836/250‑1 (MQ=255)      tGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCCTCTTATTAAG‑CCAGCAATCGAAGCTCCCCGGAGTCAGATGAAATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGGATGGc‑aga                                                                                                                                                                                                                                    >  1:805269/1‑250 (MQ=255)       gTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGc‑agaac                                                                                                                                                                                                                                  <  1:312171/251‑1 (MQ=255)       gTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGc‑agaa                                                                                                                                                                                                                                   <  1:1140201/250‑1 (MQ=255)       gTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGc‑agaa                                                                                                                                                                                                                                   <  2:416051/250‑1 (MQ=255)         ttATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGc‑agaaca                                                                                                                                                                                                                                 <  1:1111952/250‑1 (MQ=255)         ttATACTGGAGTTCGCCCTGGTCAGCTGTGTGCACTTGGATGGGAGGACCTAGATCTGCAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGCACCAAT‑AGGGTCAATCATCTTATTAAG‑CCAACAATCGACGATCTCCGGAGGCAGATGACATTAACGGAACTGAGCCAAGAGCCTATCATTTATGTT‑AACCTC‑agagagaatcgccgaaaa                                                                                                                                                                                                                                  >  2:971482/1‑238 (MQ=255)           ataCTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGc‑agaacaga                                                                                                                                                                                                                               <  2:1192407/250‑1 (MQ=255)           ataCTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCGCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGc‑agaacagaa                                                                                                                                                                                                                              <  1:809615/251‑1 (MQ=255)             aCTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGaaaa                                                                                                                                                                                                                            <  1:754641/251‑1 (MQ=255)                      cGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAAt                                                                                                                                                                                                                    <  2:1078704/250‑1 (MQ=255)                      cGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAA‑CCAGCAATCGACGCCCTCCCGAGTCAGATGACATTCACGAGACTGAGCAAAAAACATATCATTTATGTTTCACT‑C‑AGAGGGGATGGC‑AGAACAAAAAAACAAAAATg                                                                                                                                                                                                                   >  2:369672/1‑251 (MQ=255)                       gcccgtgTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAATCACAGGCGGGAACCAAT‑CGGGTCATTCAGCTTATTAAG‑CCAGCAAGCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAATCAAAAATGc                                                                                                                                                                                                                  <  2:1102739/245‑1 (MQ=255)                                aGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGtt                                                                                                                                                                                                         <  2:575001/251‑1 (MQ=255)                                    gtgtgCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTc                                                                                                                                                                                                     <  1:255462/251‑1 (MQ=255)                                      gtgCACGTGGAGGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCaa                                                                                                                                                                                                   <  2:696245/251‑1 (MQ=255)                                           cTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGAATGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTg                                                                                                                                                                                               >  1:951818/1‑250 (MQ=255)                                                   ggaggtCATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTACACGGTTCCTACAACACAGGCGGGAACCAAT‑CGGTTCATTCAGCTTATTAAG‑CCAGCAATCGACGCTCTCCGTAGTCAGATGACATGAACGAGACTGAGCAAAGAGCATAACATTGATGTC‑CACCTC‑ATAGAGTATGGC‑AGATCAGAAAAACAAAAATGCACCGTTGTTTTTCAACCTTAAGTGTCAg                                                                                                                                                                                      <  2:1025967/251‑1 (MQ=255)                                                   ggaggaCATAGATCTGAAAAATGGAACAATGCTGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAA‑CAGGTCATTCATCTTCTTAAA‑TCAGCAATCGACGCTCTCCGGAGGCAGATGACATTAACGAGACTGAGCAAAGAGGCTATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAAAAGAAAAACAAAAAAGCACCTTTGTTTTTTAACCTGAAGTGTc                                                                                                                                                                                        >  2:652533/1‑249 (MQ=255)                                                   ggaggaCATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTGTTCAACCTGAAGTGTCAg                                                                                                                                                                                      <  2:999212/251‑1 (MQ=255)                                                             gATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAACGCACCGTTGTTTTTCAACCTGAAGTGTCAGCgaga                                                                                                                                                                                 <  1:431487/246‑1 (MQ=255)                                                             gATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGGCATTCATCTTATTAAAACCA‑CAAACGACGCCCTCCGGAGTCAGATGACATTAACAAGGCTGAGCAAAGAGCCTATCATTGTTGTT‑TACCTC‑AGAGAGTATGGC‑AGAACAAAAAAAAAAAAATGCACCTTTGTTTTTTAAACTGGAGGGTCCGCgagaagaa                                                                                                                                                                             >  2:431487/1‑246 (MQ=255)                                                                 tGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCgaga                                                                                                                                                                                 <  1:105163/242‑1 (MQ=255)                                                                 tGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGAAACCGCCGCTCTCCGGAGTGAGATGACATTAAAGAGACTGAGCAAAGAGCATATCAATGATTTT‑CAACTC‑CGAGAGGATGGC‑AGAAAAAAAAAACAAAAATGCACCTTTGTTTTTCAACCTGGAGTGTccgagaga                                                                                                                                                                                 >  2:105163/1‑235 (MQ=255)                                                                  gAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTaaaaa                                                                                                                                                                          <  1:349691/248‑1 (MQ=255)                                                                  gAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCCCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAAAGCATATCATTTATGTT‑CACCTC‑AGAGAGGATGGC‑AGAACAAAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGAGTCAACGAGAGGaaaaa                                                                                                                                                                          >  2:349691/1‑248 (MQ=255)                                                                          gAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGAc                                                                                                                                                               <  1:652533/251‑1 (MQ=255)                                                                          gAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAACAAT‑CGGGGCATTCATCTTATTAAA‑CCAGCAATCGACGCCCTCCGGAGGCAGATGACATTAAAGAAACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAAAAGAAAAAACAAAATGCACCTTTGTTTTTCAACCTGAAGAGTCAACGAAAGAAAAAAAGTAttgttac                                                                                                                                                               >  2:260943/1‑245 (MQ=255)                                                                              aaTGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATACGGG‑AATTATTTTTATTTAG‑CCCGCAATCGGCGCTCTCCCGCGTCAGATGACAATAACGAGACTGAGCAAAAAGCATATCAATGATTTT‑TACCTC‑AGAGGGTATGGG‑AGAACAGAAAAACAAAAAAGCCCCTTTGTTTTTCAAACTGACATGTCAGAGAGAGAAAAAAATTATGGTGACCAtt                                                                                                                                                           >  2:1030684/1‑251 (MQ=255)                                                                               atgatgATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCAttt                                                                                                                                                          >  1:1039125/1‑251 (MQ=255)                                                                               atgatgATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAACAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑CGAGAGTATGGC‑CGAACAGAAAAACAAAAATGCACCTTTGTTTTTTAACCTGAAGTGGCAGCGAGAGGAAAAAATTATGGTGACCAttt                                                                                                                                                          >  1:303450/1‑251 (MQ=255)                                                                                  atgatCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTAc                                                                                                                                                       <  2:587253/251‑1 (MQ=255)                                                                                   tgatCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAACAAT‑CGGGTCATTTATCTTTTTAAG‑CCAGCAATCGACGCTCTCCGGGGTCAGATGACATTTACGAGACTGAGCAAAGAGCATATCATTTAAGTT‑CACCTC‑AGAGAGTATTGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTAcc                                                                                                                                                      >  1:468657/1‑251 (MQ=255)                                                                                       cAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑tt                                                                                                                                                  <  2:845711/250‑1 (MQ=255)                                                                                         ggAGAAATTTCGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAA‑AGGGTCCTTCATCTTATTAAG‑CAAGAAATCGGCGCGCTCCGGAGACAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTTATGTT‑TACATC‑AGAGAGTATGGC‑AGAACAGAAAAAAAAAAATGCACCTTTGTTTTTTAACATGAAGTGTCAGCCAAAGTAAAAAATTTTAGTGACCATTTTTACG‑TTGAc                                                                                                                                               >  2:310972/1‑251 (MQ=255)                                                                                          gagaAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCCAT‑CGGGGCATTCATCTTATTAAG‑CCAGCAAACGACGCCCTCCGGGGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTTATGTT‑AACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGAACCTTTGTTTTTTAAACTGAAGGGTTAGCGAGAGGAAAAAATTATGGTGACCCTTTTACCC‑TTGAcc                                                                                                                                              >  1:600564/1‑250 (MQ=255)                                                                                           agaAAATTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAACCAT‑CGGGTCATTCCACTTTATAAG‑CCCGCAACAGGCGCCCTCCCGAGACAAAAAACATTAAAGAGACTGAACAAAGAGCATCTCATTGCTGTT‑TACCCCCAAAG‑GTATGGCGCGAA‑AGAAAAAAAAAAAAGCACCTTTTTTTTTCAACCTGGAGCGGCAAGGAAAGTAAAAAATTATTGTGACCATTTTTCCGGTTGACt                                                                                                                                              >  2:707304/1‑251 (MQ=255)                                                                                            gaAATTTAGCAAAAGACCGTTTCACGGTACAAACAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAC‑CCAGCAATCCACGCACTCCGGAGTCAGATGACATTAACGGGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTT‑AGAGAGTATGGC‑AGAACAGAAAAAAAAAAATGCACCTTTGTTTTTCAAACTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTTCCG‑TTGACTc                                                                                                                                             >  1:801074/1‑250 (MQ=255)                                                                                                tttAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAACAAA‑CGGGGCATTCATCTTATTAAG‑CCAGCAATCGAAGCTCTCCGGAGGCAGATGAAATTAACGAGACTGAGCAAAGAGCATATCATTTATGGT‑CACCTC‑AGAGAGGAAGGA‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGGGTCAGGGAGAGTAAAAAAATATGGGGACCATTTTACCG‑TTGaataaata                                                                                                                                         >  1:1144570/1‑243 (MQ=255)                                                                                                   aGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGc                                                                                                                                     <  1:369672/251‑1 (MQ=255)                                                                                                   aGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAA‑CGGGTCATTCATTTTATTAAG‑CCAACAATCGGCGCCCTCCGGAGGCAGATGGCATTAACGAGTCTGAGCAAAGAGCATATAATTGATGTT‑TCCCCC‑AGAGAGTATGGC‑AGAAAAGAAAAAAAAAAATGCAACTTTGTTTTTCAAACTGAAGGGTCCGGGGGAGTAAAAAAATATGGTTACCATTTTTACG‑TTGACTCAATAAggg                                                                                                                                     >  2:813920/1‑250 (MQ=255)                                                                                                      aaaaGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAAACCACGCTCTCCGGAGTCCGAAGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAACGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGc                                                                                                                                     >  1:906467/1‑248 (MQ=255)                                                                                                       aaaGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGc                                                                                                                                                                                     <  1:761810/200‑1 (MQ=255)                                                                                                         aGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGAtgt                                                                                                                               <  2:96635/251‑1 (MQ=255)                                                                                                         aGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGAtgt                                                                                                                               <  2:161474/251‑1 (MQ=255)                                                                                                           aCCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAAt                                                                                                                                                                        <  2:461542/209‑1 (MQ=255)                                                                                                             cGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGa                                                                                                                           <  2:575261/251‑1 (MQ=255)                                                                                                              gTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGAc                                                                                                                          <  1:1158836/251‑1 (MQ=255)                                                                                                              gTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑ACAGCAATCGACGCTCTCCGGAGTCAGATGAAATTAACGAGACTGAGCAAAAAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAAAAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTTCCG‑TTGGCTCAATAAGGCAGATGTGGGAc                                                                                                                          >  1:353604/1‑251 (MQ=255)                                                                                                                      gTACCAAAAACACAGGCGGGAACCAAC‑AGGGCCACTCCTCTTATTAAG‑CCAGCCATCGACCCTCTCCGGAGGCAGATGCCATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAAAAGAAAAACAAAAAAGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGAGACCATTTTTCCG‑TTTACTCAATAAGGCAGGTGGGGGACGCAGCGAt                                                                                                                  >  1:918342/1‑251 (MQ=255)                                                                                                                        aCCAAAAACACAGGCGGGAAACAAA‑CGGGTCCTTCATCTTATTAAA‑ACCGCAAACGACGCTCTCCCGAGGCAGAAGACATTAAAGAGACTGAGCAAAGAGCATATCATTGATGTT‑TACCTC‑AGAGAGTATGGA‑AGAAAAAAAAAAAAAAAATGCACCTTTTTTTTTCAACCTGGAGAGTCAACGAGAGTAAAAAAATATGGTGAACATTTTACCG‑GTGACTCAATAAGGCAGATGTTGGACGCAGCGATa                                                                                                                 >  2:1067461/1‑250 (MQ=255)                                                                                                                             aaaCACAGGCGGGAACCACT‑CAGGGCATTCATGCTATTTAG‑CCAGCCATCCACGCTCTCCGGGGTCAGAAGACATTAACGAGAATGAGCCAAGAGCAACTCCTTGATGTT‑CACCTC‑AGAGAGGATGGC‑AGGACAGAAAAACAAAAATGCACCTTTGTTTTTCTACCTGGAGTGTCAACGAGAGTAAAAAATTATTGTCACCATTTTACCC‑TTGACTCAATAAGGCACATGTGGGACGCAGCGATaaaaagg                                                                                                           >  2:52612/1‑248 (MQ=255)                                                                                                                             aaaCACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAA‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAAAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAAAAAAACAAAAAAGCACCTTTGTTTTTCAAACTGAAGTGTCAGCGAGAGGAAAAAAATATGGGGACCATTTTACCG‑TTGACTCAATAAGGCAGATGGGGGAAGCAGCGATaaaaagg                                                                                                           >  2:628350/1‑248 (MQ=255)                                                                                                                               acacAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGc                                                                                                         <  2:600564/251‑1 (MQ=255)                                                                                                                                   aGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCgg                                                                                                      >  1:865464/1‑250 (MQ=255)                                                                                                                                         gAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGGGCCGGACTCCGc                                                                                               >  1:602394/1‑251 (MQ=255)                                                                                                                                                  cGGGTCATTCATCTTATTAAG‑CCAGCAATCGCCGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGTCCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGaa                                                                                        >  2:589201/1‑250 (MQ=255)                                                                                                                                                  cGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGaa                                                                                        >  2:238475/1‑250 (MQ=255)                                                                                                                                                  cGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGaa                                                                                        <  2:787742/250‑1 (MQ=255)                                                                                                                                                   gggTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGaaa                                                                                       >  2:198082/1‑250 (MQ=255)                                                                                                                                                   gggTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACATGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATACGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGaaa                                                                                       >  2:138878/1‑250 (MQ=255)                                                                                                                                                    ggTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAAt                                                                                     >  1:676898/1‑251 (MQ=255)                                                                                                                                                        attcatCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCata                                                                                 <  2:1082583/251‑1 (MQ=255)                                                                                                                                                          tcatCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCatat                                                                                <  1:101094/250‑1 (MQ=255)                                                                                                                                                                tattaAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAg                                                                             <  2:906467/247‑1 (MQ=255)                                                                                                                                                                tattaAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCg                                                                          >  1:907374/1‑250 (MQ=255)                                                                                                                                                                      g‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACAt                                                                    >  2:568935/1‑250 (MQ=255)                                                                                                                                                                                cGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGcc                                                           <  2:712262/250‑1 (MQ=255)                                                                                                                                                                                  aCGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCctgc                                                        >  2:826543/1‑251 (MQ=255)                                                                                                                                                                                   cGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCctgct                                                       >  1:518016/1‑251 (MQ=255)                                                                                                                                                                                    gCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCctgctg                                                      <  2:907374/251‑1 (MQ=255)                                                                                                                                                                                      tctcCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAACATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGTACGCAGCGATAAAACGTGCCGTACTCCGCCATCGAAAATCATATCAGTCGAGACAtcatacggcctgtgggt                                                    >  2:200517/1‑235 (MQ=255)                                                                                                                                                                                       ctcCGGAGTCATATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTc                                                   >  2:653935/1‑251 (MQ=255)                                                                                                                                                                                         ccGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCg                                                  <  1:826543/250‑1 (MQ=255)                                                                                                                                                                                         ccGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGc                                                 >  2:33818/1‑251 (MQ=255)                                                                                                                                                                                          cGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCt                                                <  1:582209/251‑1 (MQ=255)                                                                                                                                                                                           ggAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTg                                               <  2:951818/251‑1 (MQ=255)                                                                                                                                                                                              gTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACa                                            <  2:1039125/251‑1 (MQ=255)                                                                                                                                                                                                cAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGc                                          >  1:823889/1‑251 (MQ=255)                                                                                                                                                                                                  gATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTg                                        <  2:1136579/251‑1 (MQ=255)                                                                                                                                                                                                        nttAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CCCCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTTGTACGCAGCGATAAAACGTGCCGGCCTCCGCCATCGAAAATCATATCAGTCGAGACATACTTCTGCCTGCAGGTCGCTAACCGCTgtagct                                   >  2:767230/2‑245 (MQ=255)                                                                                                                                                                                                           aaCGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAAc                                <  1:928691/250‑1 (MQ=255)                                                                                                                                                                                                                gaCTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGc                           >  2:518819/1‑250 (MQ=255)                                                                                                                                                                                                                  cTGAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCAtt                        >  1:864012/1‑251 (MQ=255)                                                                                                                                                                                                                    gAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCAttt                       <  1:62322/250‑1 (MQ=255)                                                                                                                                                                                                                    gAGCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTta                      <  2:801074/251‑1 (MQ=255)                                                                                                                                                                                                                      gCAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTtat                     >  1:917033/1‑250 (MQ=255)                                                                                                                                                                                                                       cAAAGAGCATATCATTGATGTT‑CACCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTtat                     >  2:913249/1‑249 (MQ=255)                                                                                                                                                                                                                                       gATGTT‑CCCCTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAGACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGcc    <  1:138878/250‑1 (MQ=255)                                                                                                                                                                                                                                        aTGTT‑CACCTC‑AGAGAGTATGGC‑AGCACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCAt  >  1:812269/1‑251 (MQ=255)                                                                                                                                                                                                                                        aTGTT‑CACCAC‑AGAGAGAATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGAGTCAGCGAGAGAAAAAAATTATGGTGACCATTTTACCG‑TGGACTCATTAAGGCAGATATGGGACGCAGCGATAAAAAGGGCCGGACGCCGCCATCAAAAATCATATAAGCCGAGACATACTTATGCCTCCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCAt  >  2:710070/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      GTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAG‑CCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACTTC‑AGAGAGTATGGC‑AGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCG‑TTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCAT  >  NC_000913/1652094‑1652573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑