Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091329,4860CA29.8% 101.2 / 18.8 47intergenic (+291/‑165)dapB/carA4‑hydroxy‑tetrahydrodipicolinate reductase/carbamoyl‑phosphate synthetase small subunit
Reads supporting (aligned to +/- strand):  ref base C (31/2);  new base A (0/14);  total (31/16)
Fisher's exact test for biased strand distribution p-value = 3.51e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATAAAGGGCTTTAATTTTTGGCCCTTTTATTTTTGGTGTTATGTTTTTAAATTGTCTATAAGTGCCAAAAATTACATGTTTTGTCTTCTGTTTTTGTTGTTTTAATGTAAATTTTGACCATTTGG‑TCCACTTTTTTCTGCTCGTTTTTATTTCA‑TGCAATCTTCTTGCTGCGCAAGCGTTTTCCA‑GAA‑CAGGTTAGAT‑GATCTTTTTGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAAAATAATATAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAGGGTGTTTTGATTAAGTCAGCGCTATTGGTTCTGGAAGACGGAACCCAGTTTCACGGTCGGGCCATAGGGGCAACAGGTTCGGCGGTTGGGG  >  NC_000913/29246‑29735
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                          
ataAAGGGCTTTAATTTTTGGCCCTTTTATTTTTGGTGTTATGTTTTTAAATTGTCTATAAGTGCCAAAAATTACATGTTTTGTCTTCTGTTTTTGTTGTTTTAATGTAAATTTTGACCATTTGG‑TCCACTTTTTTCTGCTCGTTTTTATTTCA‑TGCAATCTTCTTGCTGCGCAAGCGTTTTCCA‑GAA‑CAGGTTAGAT‑GATCTTTTTGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAAAATAatat                                                                                                                                                                                                                                                  >  1:815457/1‑251 (MQ=255)
  aaaGGGCTTTAATTTTTGGCCCTTTTATTTTTGGTGTTATGTTTTTAAATTGTCTATAAGTGCCAAAAATTACATGTTTTGTCTTCTGTTTTTGTTGTTTTAATGTAAATTTTGACCATTTGG‑TCCACTTTTTTCTGCTCGTTTTTATTTCA‑TGCAATCTTCTTGCTGCGCAAGCGTTTTCCA‑GAA‑CAGGTTAGAT‑GATCTTTTTGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAAAATAAtata                                                                                                                                                                                                                                                 >  1:541908/1‑250 (MQ=255)
     gggCTTTAATTTTTGGCCCTTTTATTTTTGGTGTTATGTTTTTAAATTGTCTATAAGTGCCAAAAATTAAATGTTTTGTCTTCTGTTTTTGTTGTTTTAATGTAAATTTTGACCATTTGG‑TCCACTTTTTTCTGCTCGTTTTTATTTCA‑TGCAATCTTCTTGCTGCGCAAGCGTTTTCCA‑GAA‑CAGGTTAGAT‑GATCTTTTTGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAAAATAATATaaaa                                                                                                                                                                                                                                              >  1:1089793/1‑250 (MQ=255)
             aTTTTTGGCCCTTTTATTTTTGGTGTTATGTTTTTAAATTGTCTATAAGTGCCAAAAATTACATGTTTTGTCTTCTGTTTTTGTTGTTTTAATGTAAATTTTTACCATTTGG‑TCCACTTTTTTCTGCTCGTTTTTATTTCA‑TGCAATCTTCTTGCTGCGCAAGCGTTTTCCA‑GAA‑CAGGTTAGAT‑GATCTTTTTGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAAAATAATATAAAAAATCCC‑Gcc                                                                                                                                                                                                                                    <  1:349860/251‑1 (MQ=255)
                               tttGGTGTTATGTTTTTAAATTGACAATAAGTGCCAAAAATTACATGTTTTGTCTTCTGTTTTTGTTGTTTTAATGTAAATTTTGACCATTTGG‑TCCACTTTTTTCTGCTCGTTTTTATTTCA‑TGCAATCTTCTTGCTGCGCAAGCGTTTTCCA‑GAA‑CAGGTTAGAT‑GATCTTTTTGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAAATTAATATAAAAAATCCC‑GCCATTAAGTTGACTTTTAgc                                                                                                                                                                                                                  >  2:653254/1‑251 (MQ=255)
                                ttGGAGTTATGTTTTTAAATTGTCTATAAGTGCCAAAAATTACATGTTTTGTCTTCTGTTTTTGTTGTTTTAATGTAAATTTTGACCATTTGT‑TCCACTTTTTTCTGCTCGTTTTTATTTCA‑TGCAATCTTCTTGCTGCGCAAGCGTTTTCCA‑GAA‑CAGGTTAGAT‑GATCTTTTTGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAATATAATATAAAAAATCCC‑GCCATTAAGTTAACTTTTAgcg                                                                                                                                                                                                                 >  2:946567/1‑251 (MQ=255)
                                       tATGTTTTTAAATTGTCTATAAGTGCCAAAAATTACATGTTTTGTCTTCTGTTTTTGTTGTTTTAATGTAAATTTTGACCATTTGG‑TCCACTTTTTTCTGCTCGTTTTTATTTCA‑TGCAATCTTCTTGCTGCGCAAGCGTTTTCCA‑GAA‑CAGGTTAGAT‑GATCTTTTTGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAAAATAATATAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCatat                                                                                                                                                                                                          >  2:32906/1‑251 (MQ=255)
                                          ggttttAAATTCTCTATAAGTGCCAAAACTTACATGTTTTGTCTTCTGTTTTTGTTGTTTTAATGTAAATTTTGACCATTTGG‑TCCACTTTTTTTTGCTCGTTTTTATTTCA‑TGCAATCTTCTTGCTGCGCAAGCGTTTTCCA‑GAC‑CAGGTTAGAT‑GATCTTTTTGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACACAATAATATAAAAACTCCA‑GCCATTAAGTTGACTTTTAGCGCCCATAtctc                                                                                                                                                                                                       >  2:648818/3‑251 (MQ=255)
                                                  aatGTCTATAAGTGCCAAAAATTCCATGTTTTGTCTTCTGTTTTTGTTGTTTTAATGTAAATTTTGACCATTTGG‑TCCACTTTTTTCTGCTCGTTTTTATTTCA‑TGCAATCTTCTTGCTGCGCAAGCGTTTTCCA‑GAA‑CAGGTTAGAT‑GATCTTTTTGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAAAATAATATAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgc                                                                                                                                                                                               >  2:20435/3‑251 (MQ=255)
                                                       cTATAAGTGCCAAAAATTACATGTTTTGTCTTCTGTTTTTGTTGTTTTAATGTAAATTTTGACCATTTGG‑TCCACTTTTTTCTGCTCGTTTTTATTTCA‑TGCAATCTTCTTGCTGCGCAAGCGTTTTCCA‑GAA‑CAGGTTAGAT‑GATCTTTTTGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAAAATAATATAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGccgccg                                                                                                                                                                                          >  2:677922/1‑251 (MQ=255)
                                                         ataAGTGCCAAAAATTACATGTTTTGTCTTCTGTTTTTGTTGTTTTAATGTAAATTTTGACCATTTGG‑TCCACTTTTTTCTGCTCGTTTTTATTTCA‑TGCAATCTTCTTGCTGCGCAAGCGTTTTCCA‑GAA‑CAGGTTAGAT‑GATCTTTTTGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAAAATAATATAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGtt                                                                                                                                                                                        >  1:699523/1‑251 (MQ=255)
                                                          cttccgatataaaTTACATGTTTTGTTTTTTTTTTTTTTTTTTTTAATGTAATTTTTGCCCTTTTGG‑CCCTTTTTTTTCTGCTCTTTTTTATTTCA‑TGAAATTTTCTTGCGGCGCAAGCGTTTCCAA‑AAA‑CAGGTTAAAT‑GATTTTTTTGTCGTTTAATGCC‑TAAAAAA‑AATGCATGACCCAAAAAAAAAAAAAAAAAACCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGccgccg                                                                                                                                                                                          <  2:76061/238‑1 (MQ=255)
                                                                    aaaTTACATGTTTTGTCTTCTGTTTTTGTTGTTTTAATGTAAATTTTGACCATTTGG‑TCCACTTTTTTCTGCTCGTTTTTATTTCA‑TGCAATCTTCTTGCTGCGCAAGCGTTTTCCA‑GAA‑CAGGTTAGAT‑GATCTTTTTGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAAAATAATATAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGccgccg                                                                                                                                                                                          >  1:76061/1‑238 (MQ=255)
                                                                       ttACATGTTTTGTCTTCTGTTTTTGTTGTTTTAATGTAAATTTTGACCATTTGG‑TCCACTTTTTTCTGCTCGTTTTTATTTCA‑TGCAATCTTCTTGCTGCGCAAGCGTTTTCCA‑GAA‑CAGGTTAGAT‑GATCTTTTTGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAAAATAATATAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgt                                                                                                                                                                          >  2:1168424/1‑251 (MQ=255)
                                                                         ccttttttttttttttGTTTTTTTTTTTTTAATGTAATTTTTGACCTTTTGT‑CCCTTTTTTTTCTGCTCGTTTTTTTTTCA‑TGCAATCTTCTTGCTGCGCAAGCTTTTCCCA‑GAA‑CAGGTTAGAT‑GATTTTTTTGTCGTTAAATGCT‑TAAAAAA‑CATGCATGACCCAAAAAATAATAAAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtc                                                                                                                                                                         <  1:677922/235‑1 (MQ=255)
                                                                         aCATGTTTTGTCTTCTGTTTTTGTTGTTTTAATGTAAATTTTGACCATTTGG‑TCCACTTTTTTCTGCTCGTTTTTATTTCA‑TGCAATCTTCTTGCTGCGCAAGCGTTTTCCA‑GAA‑CAGGTTAGAT‑GATCTTTTTGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAAAATAATATAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAACTtcgtcg                                                                                                                                                                        >  2:85107/1‑251 (MQ=255)
                                                                                      ttttttttttttttttTAATGAAAATTTTGCCCTTTTGG‑CCCACTTTTTTCTGCTCTTTTTTATTTCA‑TGCAATCTTCTTGCTGCGAAAGC‑TTTTCCA‑AAAACAGTTTAGTT‑GATTTTTTTGCCGTTTAAT‑CC‑TTTAAAAACATGAATGAGCCAAAAAAAAATAAAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAt                                                                                                                                                              <  1:1067889/244‑1 (MQ=255)
                                                                                                      ttaataaaaTTTTTGCCATTTGGGTCCTTTTTTTTCTTCGTGTTTTTATTCCA‑GGAATTCTTCTTGCGGCGCAAGTGTTTTCCA‑AAA‑CGGGTTAGAT‑TATTTTTTTGTCGCTTAATGCC‑TGAAAAA‑CATGAAGGAGCCAAAAAATAATAAAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                                                                                                                             <  1:900556/244‑1 (MQ=255)
                                                                                                       aaTGTAAATTTTGACCATTTGG‑TCCACTTTTTTCTGCTCGTTTTTATTTCA‑TGCAATCTTCTTGCTGCGCAAGCGTTTTCCA‑GAA‑CAGGTTAGAT‑GATCTTTTTGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAAAATAATATAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatc                                                                                                                                          >  1:72713/1‑251 (MQ=255)
                                                                                                           tAAATTTTGACCATTTGG‑TCCACTTTTTTCTGCTCGTTTTTATTTCA‑TGCAATCTTCTTGCTGCGCAAGCGTTTTCCA‑GAA‑CAGGTTAGAT‑GATCTTTTTGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAAAATAATATAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtg                                                                                                                                      >  1:823870/1‑251 (MQ=255)
                                                                                                               ttttGACTATTTGT‑CCCATTTTTTTCTTCT‑TTTTTTTTTTCAATGCATTCTTTTTCCTGCACAAGCTTTTACCA‑GAA‑‑AGGTTTGATTGTTCTTTTTGGCTTTTAATGCC‑TTAAAAA‑CATGAATGACCAAAAAAAAAAAAAAAAAAACCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGa                                                                                                                                   <  1:696168/250‑1 (MQ=255)
                                                                                                                 ttgcccttttGG‑TCCTTTTTTTTCTGCTTTTTTTTATTTCA‑TGAAATTTTCTTGCGGCGCAAGC‑TTTTCCAAGAC‑CGGGTTAGAT‑GATTTTTTTGTCGTTTAATGCC‑TGAAAAA‑CATGCAGGACCCAAAAAATAATAAAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                                                                                                                                 <  1:1168424/243‑1 (MQ=255)
                                                                                                                     ccATTTGG‑TCCACTTTTTTCTGCTCGTTTTTATTTCA‑TGCAATCTTCTTGCTGCGCAAGCGTTTTCCA‑GAA‑CAGGTTAGAT‑GATCTTTTTGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAAAATAATATAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAat                                                                                                                             >  1:533583/1‑250 (MQ=255)
                                                                                                                       aTTTGG‑TCCACTTTTTTCTGCTCGTTTTTATTTCA‑TGCAATCTTCTTGCTGCGCAAGCGTTTTCCA‑GAA‑CAGGTTAGAT‑GATCTTTTTGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAAAATAATATAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                                                                                                                          >  1:479160/1‑251 (MQ=255)
                                                                                                                                          tGCTCGTTTTTATTTCA‑TGCAATCTTCTTGCTGCGCAAGCGTTTTCCA‑GAA‑CAGGTTAGAT‑GATCTTTTTGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAAAATAATATAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat                                                                                                        >  1:651982/1‑251 (MQ=255)
                                                                                                                                            ccttttttttttttctggCAATCTTTTTGCTGCGCATGCGTTTCCAA‑AAA‑CAGTTTAGAT‑GATTTTTTTTCCGTTTAATGCC‑TGAAAAA‑CATGCATGACCAAAAAAATAATAAAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAata                                                                                                       <  1:704242/234‑1 (MQ=255)
                                                                                                                                            cTCGTTTTTATTTCA‑TGCAATCTTCTTGCTGCGCAAGCGTTTTCCA‑GAA‑CAGGTTAGAT‑GATCTTTTTGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAAAATAATATAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAatat                                                                                                      >  1:825836/1‑251 (MQ=255)
                                                                                                                                            cTCGTTTTTATTTCA‑TGCAATCTTCTTGCTGCGCAAGCGTTTTCCA‑GAA‑CAGGTTAGAT‑GATCTTTTTGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAAAATAATATAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGGGAGTGAatat                                                                                                      >  1:1008449/1‑251 (MQ=255)
                                                                                                                                                tttttATTTCA‑GGCATTTTTTTTTCTGCGAAATC‑TTTTCCAAGAA‑CAGGTAAGAT‑TATCTTTTTGTCGCTTAATGCT‑TAAAAAA‑CAGGAAGGAGCCAAAAAATAATAAAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATAtt                                                                                                     <  2:538709/248‑1 (MQ=255)
                                                                                                                                                 ttttATTTCA‑TGCAATCTTCTTGCTGCGCAAGCGTTTTCCA‑GAA‑CAGGTTAGAT‑GATCTTTTTGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAAAATAATATAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTctct                                                                                                 >  1:661841/1‑251 (MQ=255)
                                                                                                                                                    tATTTCA‑CGCAATCTTCTTGCTGCGCAAGCTTTTTCAA‑GAA‑AAGGTTAGAT‑GATCTTTTTGTCGTTTAAGTCC‑TAAAAAA‑AATGAATGACCAAAAAAAAAATAAAAAAAACCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTgg                                                                                               <  1:1128491/250‑1 (MQ=255)
                                                                                                                                                     aTTTCA‑TGCAATCTTCTTGCTGCGCAAGCGTTTTCCA‑GAA‑CAGGTTAGAT‑GATCTTTTTGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAAAATAATATAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTgg                                                                                               >  2:9448/1‑249 (MQ=255)
                                                                                                                                                          a‑TGCAATCTTCTTGCTGCGCAAGCGTTTTCCA‑GAA‑CAGGTTAGAT‑GATCTTTTTGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAAAATAATATAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAGGgtgt                                                                                        >  1:161753/1‑251 (MQ=255)
                                                                                                                                                               acttttcttgctgctaatggtttttCCA‑GAA‑CAGTTTAGAT‑GTTTTTTTGGTCGCTTA‑TGCCCAAAAAAA‑CAGGCATGAGCCAAAAAAAAATAAAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAGGGTGTTTTg                                                                                    <  2:661841/230‑1 (MQ=255)
                                                                                                                                                               aaTCTTCTTGCTGCGCAAGCGTTTTCCA‑GAA‑CAGGTTAGAT‑GATCTTTTTGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAAAATAATATAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAGGTGAGTGAATATTCTCTGGAGGGTGtt                                                                                       >  1:329822/1‑248 (MQ=255)
                                                                                                                                                               aaTCTTCTTGCTGCGAAGGCTTTTTCAA‑GAA‑CAGGTTAGAT‑GATCTTTTTGTCGTTAAACGCT‑TGAAAAA‑CAGGCAGGAGCCAAAAAAAAATAAAAAAAACCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAGGGTGTTTTg                                                                                    <  1:9448/251‑1 (MQ=255)
                                                                                                                                                                       agcggcgcAAGCTTTTTCCA‑AAA‑CAGGTTAGAT‑GTTTTTTTTGTCGCTTAATGCC‑TGAAAAC‑CATGCATGACCAAAAAAAAAATAAAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAGGGTGTTTTGATTaa                                                                               <  1:85107/244‑1 (MQ=255)
                                                                                                                                                                                              a‑CAGGTTAGAT‑GATCTTTTTGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAAAATAATATAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAGGGTGTTTTGATTAAGTCAGCGCTATTGGTTCTGGAAGAc                                                      >  2:781442/1‑251 (MQ=255)
                                                                                                                                                                                                  gggtAGAT‑GATCTTTTTGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAAAATAATATAAAAAATCCC‑GCCATTAAGATGACTTTTAGCGCCCATATCTCAAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATACAGTGAGTGAATATTCTCTGGAGGGTGTTTTGATTAAGTCAGCGCTATTGGTTCTGGAAGACggg                                                   >  2:1151157/4‑250 (MQ=255)
                                                                                                                                                                                                      tagtttttttttttCTCGATTAAGGCC‑TGTAAAA‑CATGCATCACACACAAA‑TAAAAAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAGGGTGTTTTGATTAAGTCAGCGCTATTGGTTCTGGAAGACGGAAc                                                 <  2:541908/241‑1 (MQ=255)
                                                                                                                                                                                                      aGAT‑GCTCTTTTTGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAAAATAATATAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGACTGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAGGGCGTTTTGATTAAGTCAGCGCTATTGGTTCTGGAAGACGGAAccc                                               >  1:763644/1‑251 (MQ=255)
                                                                                                                                                                                                           gatCTTTTTGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAAAATAATATAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAGGGTGTTTTGATTAAGTCAGCGCTATTGGTTCTGGAAGACGGAACCCAGtt                                           >  1:297997/1‑251 (MQ=255)
                                                                                                                                                                                                                 tttGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAAAATAATATAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAGGGTGTTTTGATTAAGTCAGCGCTATTGGTTCTGGAAGACGGAACCCAGTTTCACgg                                     >  1:286110/1‑251 (MQ=255)
                                                                                                                                                                                                                 tttGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAAAATAATATAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAGGGTGTTTTGATTAAGACAGCGCTATTGGTTCTGGAAGACGGAACCCAGTTTCACgg                                     >  1:942292/1‑251 (MQ=255)
                                                                                                                                                                                                                                     aaaa‑CATGCATGAGCAAAAAAATAAAATAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAGGGTGTTTTGATTAAGTCAGCGCTATTGGTTCTGGAAGACGGAACCCAGTTTCACGGTCGGGCCATAGGGGCAACa                  <  1:781442/251‑1 (MQ=255)
                                                                                                                                                                                                                                           aggaatgAGCCAAAAAATAAAAAAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAGGGTGTTTTGATTAAGTCAGCGCTATTGGTTCTGGAAGACGGAACCCAGTTTCACGGTCGGGCCATAGGGGCATCAGGTTc             <  1:1184478/247‑1 (MQ=255)
                                                                                                                                                                                                                                                   gCCACAAAATAATATAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAGGGTGTTTTGATTAAGTCAGCGCTATTGGTTCTGGAAGACGGAACCCAGTTTCACGGTCGGGCCATAGGGGCAACAGGTTCGGCGGTTg     >  2:281345/1‑251 (MQ=255)
                                                                                                                                                                                                                                                      acaAAATAATATAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAGGGTGTTTTGATTAAGTCAGCGCTATTGGTTCTGGAAGACGGAACCCAGTTTCACGGTCGGGCCATAGGGGCAACAGGTTCGGCGGTTgggg  >  1:445174/1‑251 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                          
ATAAAGGGCTTTAATTTTTGGCCCTTTTATTTTTGGTGTTATGTTTTTAAATTGTCTATAAGTGCCAAAAATTACATGTTTTGTCTTCTGTTTTTGTTGTTTTAATGTAAATTTTGACCATTTGG‑TCCACTTTTTTCTGCTCGTTTTTATTTCA‑TGCAATCTTCTTGCTGCGCAAGCGTTTTCCA‑GAA‑CAGGTTAGAT‑GATCTTTTTGTCGCTTAATGCC‑TGTAAAA‑CATGCATGAGCCACAAAATAATATAAAAAATCCC‑GCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAGGGTGTTTTGATTAAGTCAGCGCTATTGGTTCTGGAAGACGGAACCCAGTTTCACGGTCGGGCCATAGGGGCAACAGGTTCGGCGGTTGGGG  >  NC_000913/29246‑29735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑