Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,0560GA29.8% 110.8 / 20.9 57intergenic (‑119/+84)ves/spyHutD family protein Ves/ATP‑independent periplasmic chaperone
Reads supporting (aligned to +/- strand):  ref base G (36/4);  new base A (0/17);  total (36/21)
Fisher's exact test for biased strand distribution p-value = 4.29e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTT‑CATTGTTATCGTTCAGGTAACCGTGGAT‑GGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCATTTT‑ACCTTTTGCCGCT‑GGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCG‑TC‑AGG‑ATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTT  >  NC_000913/1824812‑1825297
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                         
ggatgCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTT‑CATTGTTATCGTTCAGGTAACCGTGGAT‑GGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑Gagaaaag                                                                                                                                                                                                                                                      <  2:955383/250‑1 (MQ=255)
  atgCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTT‑CATTGTTATCGTTCAGGTAACCGTGGAT‑GGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑Gagaaaagaa                                                                                                                                                                                                                                                    >  1:483648/1‑250 (MQ=255)
     cAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTT‑CATTGTTATCGTTCAGGTAACCGTGGAT‑GGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAAcc                                                                                                                                                                                                                                                <  1:476480/251‑1 (MQ=255)
      aaTGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTT‑CATTGTTATCGTTCAGGTAACCGTGGAT‑GGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAAccg                                                                                                                                                                                                                                               >  2:152375/1‑251 (MQ=255)
       aTGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTT‑CATTGTTATCGTTCAGGTAACCGTGGAT‑GGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAAccgc                                                                                                                                                                                                                                              >  1:425508/1‑251 (MQ=255)
            ggcccGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTCCAAATTTCGCGCGTTTCACCGGAAGCATTTCGCCACAGATTCCCCGCCATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCGGTTCTTTT‑AATTGTTATCGTTCAGGTAACCGTGGAT‑GGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTTTTTG‑AGAA‑GAGAAAAGAAAACCGCCGa                                                                                                                                                                                                                                           <  1:631004/245‑1 (MQ=255)
                              ccgtgtggcgggTGGGAACGTGAAAATGTCGCTTGTTTCCCGGGCAGCTTTCCCCCACAGATTCCCCGATATTTTACGCATAAAAATGTTTTCCTAACCCGCCCCCGGCCGTTTTTTTT‑CATTGTTATCGTACAGGTAGCCGTGGAT‑GGTTTTCGGCAACTGGGGTGATTAACGTTAGTGGTGCTCGGTCTTTTTTACC‑ACCTGTTTG‑AGAA‑GAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTg                                                                                                                                                                                                                       <  2:612080/241‑1 (MQ=255)
                               cGTTTTGCCGGGGGGAACGTGCAAATTCCGCGCTTTCCCCCGGCAACATTTCGCCACAAATTCACCGACTTTTTACGCATATCAAATTATTCCATCCTCTC‑CCCGGGCCGTTTTTTTTCTTTTTTATGGTTAAGTTACCCGTGGAT‑GTTTAGCGACAACTGGGGTGTTTTACGTTAGGGGGGATCAGTTTTTTTTTAC‑AACTGTTTG‑AAAA‑GAAAAAAAAAGACCGCCGATCCTGTCCACCGCATTACTGc                                                                                                                                                                                                                      <  1:947640/251‑1 (MQ=255)
                                gTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTT‑CATTGTTATCGTTCAGGTAACCGTGGAT‑GGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGc                                                                                                                                                                                                                      >  2:53077/1‑250 (MQ=255)
                                              aaCGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTT‑CATTGTTATCGTTCAGGTACCCGTGGAT‑GGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACaa                                                                                                                                                                                                        >  2:902756/1‑250 (MQ=255)
                                                cGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTT‑CATTGTTATCGTTCAGGTAACCGTGGAT‑GGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGAc                                                                                                                                                                                                     >  2:533293/1‑251 (MQ=255)
                                                        tttcccgcgttccccggccgtcaTTTCGCCACAGATCCCCCAACATTTTAGCCAAATCAAATTATTCCATGCCCCCCCCCGCCCCTTTCTTTT‑AATTTTTCGCGTGGAGGTACCCGTGGGT‑GGTTATCGGCACCTCGGGGGTTTTACGTTAGTGGTGACCGGGTTTTTTTAAC‑ACCTTTTTG‑AAAA‑GAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGt                                                                                                                                                                                              <  1:395425/229‑1 (MQ=255)
                                                          ccgcgctTTTCCCCGGCAGCATTTCCCCACAGATCCCCCCACTTTTTACGCATATAAAAGTTTTCCATCCCCGCCCCCGCCCCTTTTTTTT‑CATTGTTATCGTTCAGGTACCCGGGGTT‑GTTTATCGGCAACTCGGGTGATTTACTTTAGTGGTGATCAGGTTTTTTTTAC‑AAC‑GTTTGGAGAA‑GAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCtt                                                                                                                                                                                           <  1:152375/250‑1 (MQ=255)
                                                               cgTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTT‑CATTGTTATCGTTCAGGTAACCGTGGAT‑GGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtt                                                                                                                                                                                      >  1:632545/1‑251 (MQ=255)
                                                                   tCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTT‑CATTGTTATCGTTCAGGTAACCGTGGAT‑GGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcggagaacgggagagg                                                                                                                                                                                  >  2:852539/1‑238 (MQ=255)
                                                                      ccggcacctttccGCCACAGATTCCCCGACTTTTTCCGCATATCAAAGTATTCCATCCCCCCCCCCGGCCCTTTTTTTT‑CTTTTTTATCTTTCAGGTACCCGTGGTT‑GTTTATCGGCAACTGGGGTTTTTTCCTTTAGTGGTGATAAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGc                                                                                                                                                                               <  1:983014/239‑1 (MQ=255)
                                                                           agcaTTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGAATTCCATACTCGCTCCCGGCCCGTTCTTTT‑CATTGTTATCGTTCAGGTAACCGTGGAT‑GGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCttaa                                                                                                                                                                           >  2:327472/1‑247 (MQ=255)
                                                                                   gCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTT‑CATTGTTATCGTTCAGGTAACCGTGGAT‑GGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGGTGAAA‑gcatattc                                                                                                                                                                 >  1:237070/1‑244 (MQ=255)
                                                                                          atccccgtcttttttcctcATATCAATGTTTTCCATACTCGCCCCCGTCCCGTTTTTTT‑CATTTTTATCTTTCAGGTACCCGGGGAG‑GGTTATCGGCAACTCGGGTTTTTAACGTTAGGGGTGATCGGGTTTTTTTTAA‑AACTGTTTG‑AAAA‑GAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGtt                                                                                                                                                        <  1:663102/233‑1 (MQ=255)
                                                                                          aTTCCCCGACATTTTCCGCATATCAAAGTATTCCATCCCCGCTCCCGGCCTTTTCTTTT‑CTTTTTTATCGTTCAGGTAACCGGGGAT‑GGTTATCGGCAACTCGGGTTTTTTACGTTAGTGGTGATCAGGTTTTTTTTAC‑AACTTTTTG‑AGAA‑GAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGtt                                                                                                                                                        <  1:957832/251‑1 (MQ=255)
                                                                                                 gACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTT‑CATTGTTATCGTTCAGGTAACCGTGGAT‑GGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGTTTTTTTGCTGAAA‑GATTATT‑CAGACA‑TTGCAGGc                                                                                                                                                  >  2:656171/1‑250 (MQ=255)
                                                                                                   cATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTT‑CATTGTTATCGTTCAGGTAACCGTGGAT‑GGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GGTTATT‑CAG‑CAGTTGACGGCAtt                                                                                                                                               >  1:986002/1‑251 (MQ=255)
                                                                                                    aTTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTT‑CATTGTTATCGTTCAGGTAACCGTGGAT‑GGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATT‑TAG‑CAGTTGCAGGCAttt                                                                                                                                              >  1:812290/1‑251 (MQ=255)
                                                                                                                     gTATTCCATACTCGCTCCCGGCCCGTTCTTTT‑CATTGTTATCGTTCAGGTAACCGTGGAT‑GGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATTACA‑‑CAGTTGCAGGCCTTTT‑ACCTTTTTCCGCT‑ggg                                                                                                                           >  2:348190/1‑250 (MQ=255)
                                                                                                                         tCCATACTCGCTCCCGGCCCGTTCTTTT‑CATTGTTATCGTTCAGGTAACCGTGGAT‑GGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACACGACCGGCGGTCTTCAGGTTTTTGGGTGAAAAGAT‑ATT‑TAG‑CCGCTGCAGGCATTTTTACCtttgccccttgaagtt                                                                                                                         >  2:1134094/1‑238 (MQ=255)
                                                                                                                              aCTCGCTCCCGGCCCGTTCTTTT‑CATTGTTATCGTTCAGGTAACCGTGGAT‑GGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCCTTTT‑ACCTTTTTCCGCT‑TGGCGTTTTGTc                                                                                                                  >  2:860439/1‑251 (MQ=255)
                                                                                                                                   cTCCCGGCCCGTTCTTTT‑CATTGTTATCGTTCAGGTAACCGTGGAT‑GGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGTTTCCGGCATTTT‑TCCTTTTTCCCCT‑GGCCGTTCTGTCAGAcc                                                                                                             >  2:251824/1‑250 (MQ=255)
                                                                                                                                        ggCCCGTTCTTTT‑CATTGTTATCGTTCAGGTAACCGTGGAT‑GGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGTTGCCGGCCTTTT‑ACCTTTTGCCGCT‑gggcg                                                                                                                         >  2:109502/1‑231 (MQ=255)
                                                                                                                                        ggCCCGTTCTTTT‑CATTGTTATCGTTCAGGTAACCGTGGAT‑GGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCATTTT‑ACCTTTTGCCGCT‑GGACGGTCTGTCAGACGCTtct                                                                                                        >  1:184053/1‑251 (MQ=255)
                                                                                                                                             gTTCTTTT‑CATTGTTATCGTTCAGGTAACCGTGGAT‑GGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAA‑GATTATT‑CAG‑CAGGTGCAGGCCTTTT‑ACCTTTTGCCGCT‑GGACGTTCTGTCAGACGCTTCTCaaa                                                                                                    >  2:828686/1‑250 (MQ=255)
                                                                                                                                              tttctttctttttttATCGTTCGGGTACCGGTGGAG‑GGTTATCGTCAACTGGGTTAATTTTTGTTATGGGTAATCAGG‑TTTTTTTTC‑CACTGTTTGGAGAA‑AAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCATTTT‑ACCTTTTGCCGCT‑GGACGTTCTGTCAGACGCTTCTCaaa                                                                                                    <  1:1127217/237‑1 (MQ=255)
                                                                                                                                                tttttgcATTGTTTTCGTTCAGGTGACGGGGGGT‑GGTTACCGGGACCCCGGGGGATTTAGTTTAGTGGGTAACAGGTTTTTTTAAC‑AATTGTTGG‑AGAA‑GAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCATTTT‑ACCTTTTGCCGCT‑GGACGTTCTGTCAGACGCTTCTCAAAAt                                                                                                  <  1:878193/244‑1 (MQ=255)
                                                                                                                                                       aTTGTTATCGTTCAGGTAACCGTGGAT‑GGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGGTGCAGGCATTTT‑ACCTTTTGCCGCT‑GGCCGTTCTGTCAGACGCTTCTCAAAATTTGCAtt                                                                                           >  1:47528/1‑250 (MQ=255)
                                                                                                                                                       aTTGTTATCGTTCAGGTAACCGTGGAT‑GGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTATTAAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCATTTT‑ACCTTTTTCCGCT‑GGACGTTCTGTCAGACGCTTCTCAAAATTATCAttt                                                                                          >  1:168859/1‑250 (MQ=255)
                                                                                                                                                          gTTATCGTTCAGGTAAC‑GGGGATTGTTTATCGGCAACCCGGGTAATTTACGTTAGGGGGGAGGGGGTTTTTTTTAC‑ATCTGTTGG‑AGAA‑AAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCATTTT‑ACCTTTTGCCGCT‑GGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTaaa                                                                                        <  1:327472/250‑1 (MQ=255)
                                                                                                                                                             aTCGTTCAGGTAACCGTGGAT‑GGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCATTTT‑ACCTTTTGCCGCT‑GGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGc                                                                                    >  1:65267/1‑251 (MQ=255)
                                                                                                                                                              tctttCAGGTAACCGTGGAT‑GGTTATCGGCAGCTGGGGTAATTTACGTTAGTGGTGATCAGGTTTTTTTTAC‑AACTGTTTG‑AAAA‑GAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCATTTT‑ACCTTTTGCCGCT‑GGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGc                                                                                    <  1:55971/247‑1 (MQ=255)
                                                                                                                                                                           gggggAT‑GGTTTTCGGCAACTGGGGTATTTTTGGTTAGGGGTAATGAGGTTTTTTTTAC‑AACTTTTGG‑AAAA‑AAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCATTTT‑ACCTTTTGCCGCT‑GGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCgg                                                                      <  1:348190/248‑1 (MQ=255)
                                                                                                                                                                            gTGGAT‑GGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGGTGCAGGCATTTT‑ACCTTTTTCCGCT‑GGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTTCggg                                                                     >  1:763058/1‑250 (MQ=255)
                                                                                                                                                                               gAT‑GGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCATTTT‑ACCTTTTGCCGCT‑GGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCG‑Tc                                                                 >  1:758755/1‑251 (MQ=255)
                                                                                                                                                                                 ggggttatcgaccacgggggTGATTTCCGTTAGTGGAGATCCGGCTTTTTTCAC‑ATCTGTTTG‑AGAA‑AAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCATTTT‑ACCTTTTGCCGCT‑GGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCG‑TC‑a                                                               <  2:47528/234‑1 (MQ=255)
                                                                                                                                                                                          ggCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCATTTT‑ACCTTTTGCCGCTTGGA‑GGTCTGTCAGACGCTTACCAAAATTAACATTTAATTGCTTTTTCTTTTCCGGCG‑TC‑Agggagttgt                                                      >  1:730605/1‑244 (MQ=255)
                                                                                                                                                                                             aaCTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCATTTT‑ACCTTTTTCCGCT‑GGACGTTCTTTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCG‑TC‑AGG‑ATGTTGTaa                                                   >  2:739868/1‑250 (MQ=255)
                                                                                                                                                                                                 cGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCATTTT‑ACCTTTTGCCGCT‑GGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCG‑TC‑AGG‑ATGTTGTAAAtttt                                              >  1:1100557/1‑251 (MQ=255)
                                                                                                                                                                                                      gatttaagtggtgtgggacctgtttttttttAC‑AATTGTTGG‑AAAA‑GAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCATTTT‑ACCTTTTGCCGCT‑GGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCG‑TC‑AGG‑ATGTTGTAAATTTTGTTCt                                         <  2:248095/227‑1 (MQ=255)
                                                                                                                                                                                                         ttACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAATTTTTTTGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCCTTTT‑ACCTTTTGCCGCT‑GGACGTTCTGTCAGACGCTTCTCAAAAATAACATTAAATTGCTTTTTCTGTTCCGGCG‑TC‑AGG‑ATGTTGTAAATTTTGTTCTggg                                      >  2:643500/1‑251 (MQ=255)
                                                                                                                                                                                                            acgttaggggttagcagtcttttttAA‑AATTGTTTG‑AAAAAGAGAAAAGAAATCCGCCGATCCTGTCCACCGCATTGCTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTGTTTTGGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCATTTT‑ACCTTTTGCCGCT‑GGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCG‑TC‑AGG‑ATGTTGTAAATTTTGTTCTGGGt                                     <  1:1054199/231‑1 (MQ=255)
                                                                                                                                                                                                               gtgggggtgaccagCTTTTTTTACCACCTTTTTG‑AAAA‑AAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCATTTT‑ACCTTTTGCCGCT‑GGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCG‑TC‑AGG‑ATGTTGTAAATTTTGTTCTGGGTTTcc                                 <  2:986002/238‑1 (MQ=255)
                                                                                                                                                                                                                 gtggtgATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCATTTT‑ACCTTTTGCCGCT‑GGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCG‑TC‑AGG‑ATGTTGTAAATTTTGTTCTGGGTTTCCAtg                              >  1:309637/1‑251 (MQ=255)
                                                                                                                                                                                                                   ggtgATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGGCAAGACCGGCGGGCTTAAGTTTTTTTGCTGAAA‑GATTATT‑CAA‑CAGTTGCAGGCATTTT‑ACCTTTTTCCGCT‑GGACGTTCTTTCAGACGCGTCTCCAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGCTC‑‑GG‑ATGTTGTAACTTTTGTTCTGGGTTTCCAtggt                            >  2:180393/1‑249 (MQ=255)
                                                                                                                                                                                                                   ggggATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGCCTTAAGTGTTTTTGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCATTTT‑ACCTTTTTGCGCT‑GGAAGTTCTTTCAGACGCTTCTCAAAATTTGCATTTAATTGGTTTTTTTGTTCCGCCG‑TC‑AGGTAT‑TTTTAAATTTTTTTCTGGGTTTCCAtttt                            >  2:173381/4‑248 (MQ=255)
                                                                                                                                                                                                                        cgagtttttttttAC‑AATTGTT‑A‑AGAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCATTTT‑ACCTTTTGCCGCT‑GGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCG‑TC‑AGG‑ATGTTGTAAATTTTGTTCGGGGTTTCCATGTGCGcc                        <  2:812290/244‑1 (MQ=255)
                                                                                                                                                                                                                                ttttttacacttgtggg‑agaa‑gaaaaAAGAAAGCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCATTTT‑ACCTTTTGCCGCT‑GGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCG‑TC‑AGG‑ATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGt                 <  2:483648/234‑1 (MQ=255)
                                                                                                                                                                                                                                 tttttaaaaactgttgaaaaa‑gaaaaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCATTTT‑ACCTTTTGCCGCT‑GGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCG‑TC‑AGG‑ATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGt                 <  1:860439/231‑1 (MQ=255)
                                                                                                                                                                                                                                 ttttAC‑AACTGTTTG‑AGAA‑GAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCATTTT‑ACCTTTTGCCGCT‑GGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCG‑TC‑AGG‑ATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTa               <  2:758755/250‑1 (MQ=255)
                                                                                                                                                                                                                                   ttAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCATTTT‑ACCTTTTGCCGCT‑GGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCG‑TC‑AGG‑ATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCt            >  1:709510/1‑251 (MQ=255)
                                                                                                                                                                                                                                      acatcTGTTAG‑AGAA‑GAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCATTTT‑ACCTTTTGCCGCT‑GGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCG‑TC‑AGG‑ATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCttt          <  1:428566/247‑1 (MQ=255)
                                                                                                                                                                                                                                        aaCTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCCTTTT‑TCCTTTTTCCTCT‑GAAAGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTTTTCCGGCG‑TCCAGG‑‑TGTTGTAAATTTTTTTTTGGGTTTTCATGTGCGCCAGCATGTTTGCTTTgt        >  2:531171/1‑250 (MQ=255)
                                                                                                                                                                                                                                          cTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCATTTT‑ACCTTTTGCCGCT‑GGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCG‑TC‑AGG‑ATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTgcgc      >  1:170017/1‑251 (MQ=255)
                                                                                                                                                                                                                                            gTTTG‑AGAC‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCATTTT‑ACCTTTTGCCGCT‑GGAAGGTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTTTGTTCCGGCG‑TC‑AGG‑ATGTTGTAAATTTTGTTCTGGGTTTCTCTGTGCGCCAGCATGTTAGCTTTTCGCTg    >  1:565830/1‑251 (MQ=255)
                                                                                                                                                                                                                                              ttG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCATTTT‑ACCTTTTGCCACT‑GGACGTTTTGTCAGACGCTTATCAAAATTAGATTTAAATTGCTTTTTTTGTTCCGGCG‑TC‑AGG‑ATGTTTGAATTTTTGTTCTGGGTTTCCTTTTGCGCCAGTATGtttgttttcgcttttt  >  2:803475/1‑237 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                         
GGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTT‑CATTGTTATCGTTCAGGTAACCGTGGAT‑GGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAC‑AACTGTTTG‑AGAA‑GAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAA‑GATTATT‑CAG‑CAGTTGCAGGCATTTT‑ACCTTTTGCCGCT‑GGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCG‑TC‑AGG‑ATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTT  >  NC_000913/1824812‑1825297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑