Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913786,8550AC34.1% 283.7 / 21.0 140G247G (GGT→GGG) gpmA2,3‑bisphosphoglycerate‑dependent phosphoglycerate mutase
Reads supporting (aligned to +/- strand):  ref base A (75/14);  new base C (0/46);  total (75/65)
Fisher's exact test for biased strand distribution p-value = 5.86e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 141 total aligned reads displayed.

ACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAGAATAGTTTCATTCC  >  NC_000913/786605‑787102
                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                           
aCCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTa                                                                                                                                                                                                                                                             <  2:204319/251‑1 (MQ=255)
       ccTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGt                                                                                                                                                                                                                                                     >  2:1115718/1‑251 (MQ=255)
       ccTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAAGGCAGTAAAAGCGCGTCGCGGGTGAGGTTTAATTGTCTGATGCGCCGTCAGAGTTGCGTATCCGATGAATCGCCACAGGCCTGATAAGTCGCGCAGCGTCGCAGCAGGCATTGTGGTCGTTTGTGAGGAACGAAAAAG‑CCGATTCACGTGCGGTTGGCTTTCTTGGTTTATACGAGTG‑ACGTTTGCTTCGCGTTAGC‑CTGGt                                                                                                                                                                                                                                                     <  2:922523/251‑1 (MQ=255)
           cATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑Gc                                                                                                                                                                                                                                                <  1:500582/251‑1 (MQ=255)
                     gAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCgct                                                                                                                                                                                                                                    >  1:161765/1‑251 (MQ=255)
                     gAAGATACCGATGCTCTGATACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGCATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCTCCgct                                                                                                                                                                                                                                    >  2:872973/1‑251 (MQ=255)
                        aatcccGATGCTCTTTTACGTCAACGGGCGAATGCAGAAAAAGCGCGTCGCGGGTAAGGTTTAATTGCCGGAGGCCCCGTCAGAGGGGCGTATCCGATGAATCCCCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCATTGTGCTCCATTGTTAGCAAAAAAAAAG‑CCGACTCCCTTGCAGTCGGTTTTCTCATTTTAAACGAATG‑ACTTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCgctgct                                                                                                                                                                                                                                 <  1:546500/247‑1 (MQ=255)
                           cccGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGAGGAATCACCACAGGCCTGATAAGCCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTGCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTc                                                                                                                                                                                                                              <  2:361394/250‑1 (MQ=255)
                              gATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGCCTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCt                                                                                                                                                                                                                           >  1:436840/1‑251 (MQ=255)
                              gATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAGTG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCt                                                                                                                                                                                                                           >  1:532284/1‑251 (MQ=255)
                              gATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑CA‑CCGCCGCTGCTTTCGCt                                                                                                                                                                                                                           >  2:841536/1‑251 (MQ=255)
                                      gTTACGTCAACTGGCGAATGCAGTAAAGGCGCGTCGGGGGTAAGGTTTATTTGTCGGATGCGCCGTCAGAGGGGCGTATCGGAGGAATCCCCACGGGCCTGATAAGGCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGTTTTCTCATTTTAACCGAATG‑ACGTTTACTTCTCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGAtctc                                                                                                                                                                                                                   <  1:1001076/251‑1 (MQ=255)
                                         aCGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTc                                                                                                                                                                                                                >  2:118930/1‑251 (MQ=255)
                                          cGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCCTTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTc                                                                                                                                                                                                                >  1:579338/1‑250 (MQ=255)
                                           gTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCACCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTc                                                                                                                                                                                                                >  1:890839/1‑249 (MQ=255)
                                                 gggcgatgtcAGTAAAAGGGCGGCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCGGAGGGGCGAATCCGATGAATCCCCACAGGCCGGATAAGCCGCGCAGCGTCGCATCAGGCAATGTCCTCCTGTGTTAGCAACAAAAAGG‑CCGTCTCCCTTGCAGCCGGCTTTCTCATTTTAAACGAAGG‑ACGTTTACTGCGCTTTCCC‑CTGTTTT‑GA‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTAc                                                                                                                                                                                                        <  2:25284/242‑1 (MQ=255)
                                                  ggCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTAcc                                                                                                                                                                                                       >  2:243935/1‑251 (MQ=255)
                                                   gggtatgaaacaaaaGCGGGCCGCGGGTAAGTTTTAATGGCGGGATGCGCCGTCAGGGGGGTCTATCGGAGAAACCCCCACAGGCAGGATAGGTCGGGCGGCGTCGAACCGGGAACTGTGCTCGATTGTTAGCAAAAAAAAAC‑CCGACTCCCTCGCGGCCGTGTTTCTCATTTTAAACGAATC‑GCTTTTGCTTCGCTTT‑CC‑CGTGTTT‑GCCAA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTAccc                                                                                                                                                                                                      <  2:579338/240‑1 (MQ=255)
                                                     gAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTAccc                                                                                                                                                                                                      >  2:799037/1‑249 (MQ=255)
                                                     gAATGCAGTAAAAGCGCATCGCGGGAAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTAccc                                                                                                                                                                                                      >  1:827663/1‑249 (MQ=255)
                                                          cAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGCTGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCATataat                                                                                                                                                                                               >  2:106456/1‑251 (MQ=255)
                                                          cAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGataat                                                                                                                                                                                               >  2:83917/1‑251 (MQ=255)
                                                                     gggcgggggaaaGGTTTAATTGGGGGATGGGCCGCCAGGGTGGCGTACCCGATGATACCCCACAGCCCGGATAAGCCGCGCAGCGTGGCATGGGGCATTGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACCCCCTTGCAGTCGGTTTTCTCTTTTAAAACGAATG‑ACTTTTACTTCGCTTTCCC‑CGGTTTT‑GC‑AG‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGc                                                                                                                                                                                    <  2:161765/241‑1 (MQ=255)
                                                                     cgTCGGGGGTAAGGTTTAATTGTCGTAGGCGCCGTCAGGGTGGCTTACCCGATAAATCCCCACAGGCCTGATAAGTCGCGCAGGTTCGCATCAGGAAATGTGCTCTATTGTTAGAAAAAAAAAAG‑CCGACTCCTTTGCAGTCGGTTTTCTCATTTTAAACGAATG‑ACTTTTACTTCGTTTTCCC‑CGGTTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGc                                                                                                                                                                                    <  1:1013586/251‑1 (MQ=255)
                                                                     cgTCGCGGGAAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGCTAATAGCGTTTCAGc                                                                                                                                                                                    >  1:578404/1‑251 (MQ=255)
                                                                      gTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCg                                                                                                                                                                                   >  1:586531/1‑251 (MQ=255)
                                                                      gTCGCGGGTAAGGTTTAATTGTCGGAGGCGCCGTCAGAGTGGCGAATCCGATAAACCCCCACAGGCCTGATAAGGCGCGCAGGGCCGCACCAGGCATTGTGCTCAATTGTTATCAACAAAAAAG‑CCGACTCACTTGCAGGCGGCTTTCTCATTTTAAACGAATG‑GCGTTTACTCCGCTTTACC‑CTGGTTG‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCg                                                                                                                                                                                   <  1:1067582/251‑1 (MQ=255)
                                                                        cgcgGGTAAGGTTAAATTGTGGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCCCCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTTTTAGCAACAAAAAAG‑CCGACTCTCTTGCAGTCGGCTTTCTCATTTTAAACAAATG‑ACTTTTACTTCGCTTTACC‑CGGTTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCgg                                                                                                                                                                                  <  1:684077/250‑1 (MQ=255)
                                                                          cgGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGttt                                                                                                                                                                               >  2:256526/1‑251 (MQ=255)
                                                                            ggTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCAtt                                                                                                                                                                                                          >  2:222258/1‑222 (MQ=255)
                                                                               aaGGTTTAATTTCCGGATGCGCTTCCAGAGTGGCGTACCCGATAAATCGCCACAGGCTAGATAAGTCGCGAACCTGCGCAAAAGGCAGTGTGCTCCATTGTAAAAAAAAAAAAAG‑CCGACTCAATTGAAATCGCTTTTCTCATTTTAAACGAATG‑AGGTTTTCTTCGTTTTCCC‑CAGGTTT‑CA‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCatctttttgaaa                                                                                                                                                                           <  1:106456/250‑12 (MQ=255)
                                                                               aaGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAAt                                                                                                                                                                          >  1:310597/1‑251 (MQ=255)
                                                                                    taaaTTGTGGGATGCGCCGTCAGAGGGGCGTACCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCCCCATTGTTAGCAAAAAAAAAG‑CCGCCTCCCTTGCAGTCGGTTTTCTCATTTTAAACGAATG‑ACTTTTACTTCGCTTTCCC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATtctc                                                                                                                                                                      <  1:1115718/248‑1 (MQ=255)
                                                                                            gggttgccccGTCAGAGTGGCGTATCCGATGAACCCCCCCAGTCCTGATAAGCCGGGCGTCGCCCCATCGGGCAATGTGCTCCATTTTTAGCAAAAAAAAAG‑CCGCCTCCCTTGCGGTCGGTTTTCTCATTTAAAACAAATG‑ACTTTTACTTCGCTTTCCC‑CTGTTTT‑CC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACt                                                                                                                                                              <  2:674078/242‑1 (MQ=255)
                                                                                               aTGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATACTAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCAt                                                                                                                                                           >  2:379013/1‑250 (MQ=255)
                                                                                                  cgcCGTAAGAGTGGCGTATCCGATGAATCCCCACAGGCCTGATAAGTCGCGCAGCGCCGCATCAGGCAATGTGCTCCATTTTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGTTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTCCC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATacac                                                                                                                                                       <  1:506749/251‑1 (MQ=255)
                                                                                                        cAGAGGGGCGTATCCGATGAATCCCCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCTTTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGTTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTCCC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCg                                                                                                                                                  <  1:57025/250‑1 (MQ=255)
                                                                                                           ggTGGCGTATCCGATGAATCACCACGGGCCTGATAAGTCGCGCAGCGTCGAATCGGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTCCC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCAc                                                                                                                                              <  1:227985/250‑1 (MQ=255)
                                                                                                                 gTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGata                                                                                                                                                                                                 >  1:78418/1‑194 (MQ=255)
                                                                                                                  tacccgaagaatcccccaaGGCCTAATAAGTCGCGCAGCGTCGAACCAGGCATTTTGCTCCATTTTTAGAAAAAAAAAAC‑CCGCCTCCCTTGCAGTCGGCTTTCACATTTTAAACGAATG‑ACGTTTCCTTCGCTTTCCC‑CTGTTTT‑CC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAg                                                                                                                                         <  2:1056583/231‑1 (MQ=255)
                                                                                                                   aTCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCg                                                                                                                                      >  2:1103181/1‑251 (MQ=255)
                                                                                                                   aTCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAAAAAAAAAG‑CCGACTCACTTGCAGTCGGTTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGTTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTc                                                                                                                                       <  1:504959/250‑1 (MQ=255)
                                                                                                                     ccGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCggg                                                                                                                                    >  1:19737/1‑251 (MQ=255)
                                                                                                                       ggagaaccaccagtgctcagATAAGGCGCGCGGCGCCGCATCACGCAATGTGCCCCTTTGTAAGAAAAAAAAAAC‑CCGCCTCCCTTGCAGTCGCTTTTCTCATTT‑AAACCAAAGTCCGTTCACTCCGCTTTCCC‑CGGTTTT‑CC‑AA‑CCGCCGCTGCTTTCGCTGCGATCGCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCATTCGGGat                                                                                                                                  <  2:1034161/232‑1 (MQ=255)
                                                                                                                          gatcccccacAGCCCTGAAAAGTCGCCCAGCGTCGAATCAGGAAATGTGCTCCATTTTAAGAAACAAAAAGG‑CCGACTCACTTGCGGTCGGTTTTCTCATTTTAAACTAATG‑GCGTTTACTTCGCTTTCCC‑CGGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGatat                                                                                                                                <  1:1023736/244‑1 (MQ=255)
                                                                                                                                  acaGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCa                                                                                                                        >  1:623368/1‑250 (MQ=255)
                                                                                                                                           aTAAGTCGCGCAGCGTCGCATCAGGAAATGTGCTCCATTGTTAGAAACAAAAAAG‑CCGACTCACTGGCAGTCGGCTTTCTCTTTTTAAACGAATG‑ACGTTTCCTTCGCTTTCCC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCtctt                                                                                                              <  1:808612/251‑1 (MQ=255)
                                                                                                                                             aagtcgggaaccggcGCATCGGGCAATGTGCTCCATTGTTGCCAACAAAAAGC‑CCGCCTCACTTGCGTTCGGCTTTCTCATTTTAAACGAATC‑ACGTTTACTTCGCTTCCCC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCtcttct                                                                                                            <  1:83917/237‑1 (MQ=255)
                                                                                                                                               gcccgcgggcggcgaatcgggCATTGTGCTCCATTTTAAGAAACAAAAAGG‑CCGACTCTCTTGCTGTCGGCTTTCTAATTTTAACCGGGTG‑CCGTTTACTTCGTTTTCCC‑CGGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCttcttc                                                                                                          <  2:532284/232‑1 (MQ=255)
                                                                                                                                                  gcgcgccgccgattCAGGCAATTTGCTCCATTTTTAGCAA‑AAAAAAGTCCGACTCCCTTGCATTCGTCTTTCTCATTTTAACCGAAGG‑CCGTTTACTTCTCTTTCCC‑CTGTTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCt                                                                                                       <  1:129850/238‑1 (MQ=255)
                                                                                                                                                   cgcAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTc                                                                                                      >  2:505884/1‑251 (MQ=255)
                                                                                                                                                     cAGCGTCGCATCAGTCAATGTGCTCCATTTTTAGCAAAAAAAAAG‑CCGACTCACTTGCAGTCGGTTTTCTCATTTTAAACAAATG‑ACGTTTACTCCGCTTTCCC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCAt                                                                                                    <  1:1074927/251‑1 (MQ=255)
                                                                                                                                                       gCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGt                                                                                                  >  1:1133029/1‑251 (MQ=255)
                                                                                                                                                        cGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCAtt                                                                                                                                                                                                          >  2:1132409/1‑146 (MQ=255)
                                                                                                                                                        cGTCGCATCAGGCAATGTCCCCCATTGTTAGCAAAAAAAAAC‑CCGCCTCACTTGCATTCGGTTTTCTTATTTAAAACGAATG‑ACGTTTACTTCTCTTTCCC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGtt                                                                                                 <  1:715039/251‑1 (MQ=255)
                                                                                                                                                           cGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATc                                                                                              >  1:1085422/1‑251 (MQ=255)
                                                                                                                                                              aTCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCa                                                                                             >  2:562129/1‑249 (MQ=255)
                                                                                                                                                                cAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCa                                                                                             >  2:794814/1‑247 (MQ=255)
                                                                                                                                                                   gCAATGTGCTCAATTGTTAGCAAAAAAAAAG‑CCGCCTCCCTTGCAGTCGGTTTTCTCATTTTAAACGAATG‑ACGTTTCCTCCGCTTTCCC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAtt                                                                                      <  1:921607/251‑1 (MQ=255)
                                                                                                                                                                    aaaTGTCCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGTTTTCTCATTTTAAACGAAGG‑CCTTTTCCTTCCCTTTCCC‑CTGGTTT‑AC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt                                                                                     <  1:393834/250‑1 (MQ=255)
                                                                                                                                                                     aaTGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTc                                                                                    >  2:47536/1‑251 (MQ=255)
                                                                                                                                                                     aaTGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTc                                                                                    >  2:242623/1‑251 (MQ=255)
                                                                                                                                                                        gttctcaattgttacaaaaaaaaaaG‑CCGACTCACTTGCATGCGTCTTTCTTATTTTAAACGATTG‑ACTTTTCCTTCGCTTTCCC‑CTGTTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCAc                                                                                  <  2:629002/231‑1 (MQ=255)
                                                                                                                                                                             ctatttttaacaaaaaaaaaC‑CCGCCTCACTTGCGGGCGTTTTTCTATTTTTAAACGAATG‑ACGTGTACTTCGTTTTCCC‑CTGTTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTAGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAgc                                                                              <  2:858872/235‑1 (MQ=255)
                                                                                                                                                                             ccATTGTTAGAAAAAAAAAAG‑CCGACTCACTTGCATCCGGTTTTCTCATTTAAAACGATTG‑CCGTTTACTTCTTTTTCCC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAgcgc                                                                            <  1:1141838/251‑1 (MQ=255)
                                                                                                                                                                               aTTGTTATCAACAACAAAG‑CCGACTCACCTGCAGTCGGCTTTCTCATTTTAAACGAAAG‑CAGTTTACTTCGCTTTACC‑ATGGTTT‑GC‑AA‑CCGCCGATGCCTTCGCTGCGATCGAGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTACGATCCAGAATATTTTCTTCGCTCATGTTATCACGATATTCCACCAGCGCAc                                                                          >  2:166395/1‑251 (MQ=255)
                                                                                                                                                                                 tGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGt                                                                        >  1:63083/1‑251 (MQ=255)
                                                                                                                                                                                  gTTAGCAACACAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGt                                                                        >  2:262671/1‑250 (MQ=255)
                                                                                                                                                                                       caacaaAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCAAACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAag                                                                    >  2:347584/1‑249 (MQ=255)
                                                                                                                                                                                        aacaaAAAAG‑CCGACTCACTTGCAGTCGGTTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTCCC‑CTGTTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGt                                                                 <  1:256526/251‑1 (MQ=255)
                                                                                                                                                                                          caaAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGtt                                                                >  1:274791/1‑250 (MQ=255)
                                                                                                                                                                                            aaaaaG‑CCGCCGCCCTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGTTTTCCC‑CTGTTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTAcc                                                             <  1:238429/251‑1 (MQ=255)
                                                                                                                                                                                             aaaaagccgtcacatgtGCAGCCGGTTTTCTCATTTTAAACAAATG‑CTGTTCACTTCTCTTT‑CC‑CCGGTTT‑GC‑AAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTAcc                                                             <  2:13944/235‑1 (MQ=255)
                                                                                                                                                                                             aaaaG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCg                                                            >  1:244693/1‑251 (MQ=255)
                                                                                                                                                                                                aG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCgtgt                                                         >  2:691143/1‑251 (MQ=255)
                                                                                                                                                                                                     ccctcccttccAGTCGGTTTTCCCATTTTAAACGAATT‑GCGTTTACTTCGCTTTCCC‑CTGTTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAg                                                     <  2:412876/241‑1 (MQ=255)
                                                                                                                                                                                                       cTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCg                                                   >  1:531965/1‑251 (MQ=255)
                                                                                                                                                                                                         cACTTGCAGTCGGCTTTCTCATTTTAAACGACTG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGAGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCgat                                                 >  1:422835/1‑251 (MQ=255)
                                                                                                                                                                                                            ttGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTc                                                                                                                                                             >  1:144384/1‑140 (MQ=255)
                                                                                                                                                                                                              gCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATc                                             >  1:509065/1‑250 (MQ=255)
                                                                                                                                                                                                                agtgggttttatcATTTTAACCGAAGG‑ACTTTTACTTCGCTTTCCC‑GTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCAc                                           <  1:872973/239‑1 (MQ=255)
                                                                                                                                                                                                                 gTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCAcgc                                         >  1:112429/1‑251 (MQ=255)
                                                                                                                                                                                                                  tgggCTTTCTTATTTTAAACGAGTG‑CCTTTTCCTCCG‑TTTGCCGCTGTTTC‑GC‑AC‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCAcgc                                         <  2:547202/248‑1 (MQ=255)
                                                                                                                                                                                                                  tCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCAcgcg                                        >  2:756919/1‑251 (MQ=255)
                                                                                                                                                                                                                  ccgtttttCTCTTTTAAAACGAATG‑ACGTTTACTTCGCTTTCCC‑CTGGTTT‑GA‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCAcgcg                                        <  1:756919/246‑1 (MQ=255)
                                                                                                                                                                                                                     gCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTc                                     >  1:1095268/1‑251 (MQ=255)
                                                                                                                                                                                                                        ttCTCATTTTAACCGGATG‑ACGTTTACTTCGCTTTACC‑CTGGCTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTAGCTCATGTTATCAAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCAGCGATGATCACGCGCTCAcc                                  >  2:454039/1‑251 (MQ=255)
                                                                                                                                                                                                                         tctcATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCg                                 >  2:333285/1‑251 (MQ=255)
                                                                                                                                                                                                                          ctcATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGc                                >  2:645501/1‑251 (MQ=255)
                                                                                                                                                                                                                               tttaaaaaaaatgtcttttACTCCGCTTT‑CC‑CTGGTTTTGC‑AG‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCAAACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGctct                             <  1:347584/234‑1 (MQ=255)
                                                                                                                                                                                                                                ttAAACGAATG‑CCGTTTCCTTCGTTTTCCC‑CTGTTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTc                           <  1:691143/250‑1 (MQ=255)
                                                                                                                                                                                                                                  aaaCGATTG‑ACGTTTACTTCGTTTTCCC‑CGGTTTT‑GA‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCAt                         <  1:580470/250‑1 (MQ=255)
                                                                                                                                                                                                                                      gAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATAcgcg                    >  1:18967/1‑251 (MQ=255)
                                                                                                                                                                                                                                       aaTG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCgg                   >  2:186509/1‑251 (MQ=255)
                                                                                                                                                                                                                                        aaggctgTTTACTTCCCTTTCCC‑CGGGTTT‑‑C‑AAGCCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCgg                   <  1:213070/245‑1 (MQ=255)
                                                                                                                                                                                                                                            aCGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGCTATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGc                  >  2:960886/1‑248 (MQ=255)
                                                                                                                                                                                                                                              gTTTACTTCTCTTCACC‑CTGTTTT‑GC‑AC‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAGAAt             <  2:1075512/251‑1 (MQ=255)
                                                                                                                                                                                                                                                   cTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAGAATAGttt        >  2:1175146/1‑251 (MQ=255)
                                                                                                                                                                                                                                                         tttACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAGAATAGTTTCATTcc  >  2:819886/1‑251 (MQ=255)
                                                                                                                                                                                                                                                           tACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAGAATAGTTTCATTcc  >  1:707276/1‑249 (MQ=255)
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ACCGATGCCTGCATCGGCTGGGAAGATACCGATGCTCTGTTACGTCAACTGGCGAATGCAGTAAAAGCGCGTCGCGGGTAAGGTTTAATTGTCGGATGCGCCGTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATG‑ACGTTTACTTCGCTTTACC‑CTGGTTT‑GC‑AA‑CCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAGAATAGTTTCATTCC  >  NC_000913/786605‑787102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑