BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,558,907	T→A	K207M (AAG→ATG)	iadA ←	isoaspartyl dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,558,907	0	T	A	95.7%	439.6 / NA	141	K207M (AAG→ATG)	iadA	isoaspartyl dipeptidase
Reads supporting (aligned to +/- strand): ref base T (0/6); new base A (66/68); total (66/75)
Fisher's exact test for biased strand distribution p-value = 2.94e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 142 total aligned reads displayed.

GCTACCGTTGCCGTCGGAGCTGAGGGTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTC  >  NC_000913/4558660‑4559151

      gTTGCCGTCGGAGCTGAGGGTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGt                                                                                                                                                                                                                                              >  1:94460/1‑251 (MQ=255)

      gTTGCCGTCGGAGCTGAGGGTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGt                                                                                                                                                                                                                                              >  2:803382/1‑251 (MQ=255)

         gCCGTCGGGGCTGAGGGTGCCGCGTGCCAGCGGAATCCCCGCCTGAACGGCGCGGGCAATACCTTCGGCGGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCg                                                                                                                                                                                                                                            <  2:20428/250‑1 (MQ=255)

         gCCGTCGGAGCTGAGGGTGACGCGTGCCAGCGGAATACCCGCCTGACCGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGc                                                                                                                                                                                                                                           <  2:440084/251‑1 (MQ=255)

            gTCGGAGCTGAGGGTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGcccc                                                                                                                                                                                                                                        >  2:484695/1‑250 (MQ=255)

                  gCTGAGGGTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGa                                                                                                                                                                                                                                  <  1:131976/251‑1 (MQ=255)

                         gTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGAGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                                                                                                                                                                                                                           >  1:473225/1‑251 (MQ=255)

                                    cAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑Gtt                                                                                                                                                                                                               <  2:682673/251‑1 (MQ=255)

                                         gAATCCCCGCCTGGACGGCGCGGGCAATACCTTCGGCTGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCTCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTAccg                                                                                                                                                                                                          <  2:649288/251‑1 (MQ=255)

                                           acaCCCGCCTGAACGGCTCGGGCAATACGTGCGGAAGGGGCGATCGGGTCGTAAATGCTGCTGGTGAGTTCGATTGTGCCGCCTTGGCGCGCGAACGCCATCGCTTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGAAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGTAACACGGTGACGCCAG‑GTTTAccgcc                                                                                                                                                                                                        <  2:699802/249‑1 (MQ=255)

                                           aTACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTAccgcc                                                                                                                                                                                                        >  1:588285/1‑251 (MQ=255)

                                           aTACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTAccgcc                                                                                                                                                                                                        >  2:698869/1‑251 (MQ=255)

                                             aCCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTAccgccg                                                                                                                                                                                                       >  1:683097/1‑250 (MQ=255)

                                               ccGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGc                                                                                                                                                                                                    >  2:191858/1‑251 (MQ=255)

                                                 gCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGCACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCa                                                                                                                                                                                                   >  1:503020/1‑250 (MQ=255)

                                                 gCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAAAGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGAGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGC‑CGGCAGCAGCTTGCTGAACGGCACATCGCAGATTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGGGGAACACGGTGACGCCTG‑GTTTAccaccaagcc                                                                                                                                                                                                   >  2:679389/1‑242 (MQ=255)

                                                   cTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GATTACCGCCGAGCAAAc                                                                                                                                                                                                >  1:444446/1‑251 (MQ=255)

                                                    tGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAAcc                                                                                                                                                                                               >  1:376793/1‑251 (MQ=255)

                                                     gAACGGCGCGGGCAATTCCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAAcc                                                                                                                                                                                               <  1:464939/250‑1 (MQ=255)

                                                      aaCGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGc                                                                                                                                                                                             >  1:766968/1‑251 (MQ=255)

                                                                 cAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACGCGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGAt                                                                                                                                                                                  <  1:715710/251‑1 (MQ=255)

                                                                 cAATAACTTCGGCAGGAGGGCCCGGTGCGTCAATGCTGCTGGTGCTATCGATGGTGCCGCCTTAGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGAACGTTGCGGTTAACGTGGTG‑CGGCAGACGATTGCAAATCGGCACATCGCAGGTTTCCAGCAGGT‑CATAAAAAGGGTGTAACGCCACTTTACTGTCGCCCAAGTGGAACACGGTGACGCCAG‑GTTTAACGACGAGAAAACCGCCAACCCGGGa                                                                                                                                                                                   >  2:236058/1‑250 (MQ=255)

                                                                     aCCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTg                                                                                                                                                                              <  1:816933/251‑1 (MQ=255)

                                                                      ccTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTgc                                                                                                                                                                             <  1:364341/251‑1 (MQ=255)

                                                                              gggggCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGAGCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCTGAGCAAACCGCCAACGCGGGATTCTGCCGCCatat                                                                                                                                                                     <  1:608414/250‑1 (MQ=255)

                                                                                  gCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGc                                                                                                                                                                 >  1:290074/1‑251 (MQ=255)

                                                                                    gACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGcc                                                                                                                                                                >  1:318334/1‑250 (MQ=255)

                                                                                            cGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACACCGGTACGATGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCAAG‑GTTTACCGCAGAGCAAACCGCCAACGCGGGATTCTGCCGCCATCTTGGACAGGTGATa                                                                                                                                                        >  1:731638/1‑250 (MQ=255)

                                                                                              tCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGaaaacc                                                                                                                                                     >  2:175831/1‑246 (MQ=255)

                                                                                               cAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACg                                                                                                                                                    >  1:655529/1‑251 (MQ=255)

                                                                                                 aTGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGG‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTTACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCTCCATATTGGCCAGGTGATAAACGTc                                                                                                                                                  <  1:641290/251‑1 (MQ=255)

                                                                                                        tggggataccggtggggccaccTTTGCGCGGGAACGCCAGCGCCTGCTCAAACAACGGTACGTTGCTGTTAACGTGGGT‑CGGCAGCAGCTTTCTGTTCGGCACATCTCATTTTTCCAGCAGGC‑CATAAATAGGCTGTGACGCCATTTTACGGCCGCCCTTGTGGAACACGGTGCTGCCGG‑TTTTACCGCGGAGAAAACCGCCAACGGGGGATTCGGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGc                                                                                                                                            <  2:691170/230‑1 (MQ=255)

                                                                                                        gggTGATATCGAGGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCg                                                                                                                                           <  1:194886/250‑1 (MQ=255)

                                                                                                         ggTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGc                                                                                                                                            <  1:105044/249‑1 (MQ=255)

                                                                                                         ggTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCgg                                                                                                                                          >  1:314137/1‑251 (MQ=255)

                                                                                                                      gggccgccTTGGCGCGCGAACTCCAGCGCCTGCTCAAACAACGTTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCGGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATc                                                                                                                             <  2:766968/249‑1 (MQ=255)

                                                                                                                      gTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATCTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATc                                                                                                                             <  1:634429/251‑1 (MQ=255)

                                                                                                                        gccgccTTTGCGCGCGAACCCCAGCGCCTGCCCAAACAACGGTCCGTTGCGGTTAACGTGGGC‑CGGCAGCAGGTTGTTGGTCGGCACCTCGCAGTTTTCCAGCAGGT‑AATAAATAGGCTGTAACGCCATTTTACTGTCGCCAATGTGGAACGCGGTGACGCGGG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCag                                                                                                                           <  1:67176/251‑1 (MQ=255)

                                                                                                                        cccgccTATGCGAGCTAACCCCAGCGCGCGTTCAAACAACGGTCCGTGTCAGTTACGGCGGGC‑CGGCGGCAGCTTGCGAAACGGCAAACCGCAGTTTTC‑AGCCGGTGCAAAACTAGGCTGTAACGCCATTTTACTGTCCCCCATGTGGACCCAGGGGGCGC‑AGCGTTTACGGCCGAGCAAACCGCCAACGCGGGGTTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCTGCAGAACGGTGATCat                                                                                                                           <  2:813147/250‑2 (MQ=255)

                                                                                                                           gccTTTGCGCGCTAACTCAGGCGCCTGCTCAAACAACGGTCCGTTGCGGTTAA‑GGGGGTGCGGCGGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGGGGAACACGGTGACGCCAG‑TTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAgaga                                                                                                                        <  2:704915/251‑1 (MQ=255)

                                                                                                                            ccTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCag                                                                                                                           >  2:614146/1‑247 (MQ=255)

                                                                                                                             cTTTGCGCGCGAACTCCAGCGCCTGCTCAACCAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTCATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGACCATGTGGAACACGGCGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGGGATAAACGTTCGGTGCGGCAGAACGGTGATCAGAGATg                                                                                                                      >  1:337401/1‑251 (MQ=255)

                                                                                                                             cTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATg                                                                                                                      >  1:208405/1‑251 (MQ=255)

                                                                                                                             cTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATg                                                                                                                      >  1:715343/1‑251 (MQ=255)

                                                                                                                             cTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATg                                                                                                                      >  1:772229/1‑251 (MQ=255)

                                                                                                                                 gcgcgcGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGgcg                                                                                                                   <  1:698869/250‑1 (MQ=255)

                                                                                                                                  cgcgcgAACTCCAGCGCCTGCTCAACCAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCa                                                                                                                 <  1:388899/251‑1 (MQ=255)

                                                                                                                                  cgcgcgAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGgcgc                                                                                                                  >  1:362136/1‑250 (MQ=255)

                                                                                                                                   gcgcgAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGTT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGAGATAAACGTCCGGGGCGGCAGAACGGTGATCAGAGATGGCGCAt                                                                                                                >  2:758202/1‑251 (MQ=255)

                                                                                                                                     gcgAACGCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGGGGTT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATGGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACAGGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCAttt                                                                                                              <  2:549464/251‑1 (MQ=255)

                                                                                                                                     ccgaccTCCAGCGCCTGCTCAACCACCGGTCCGTTGCGGTTACTGTGGGT‑CGGAGGCAGTTTGCGGATCGGCCCATCCCGGTTTTCCAGCAGGT‑CATAAATAGTCTGTAACGCCATTTTACTGTCGCCCATGGGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCAttt                                                                                                              <  2:832045/246‑1 (MQ=255)

                                                                                                                                        aacgccAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCAc                                                                                                           <  1:683998/247‑1 (MQ=255)

                                                                                                                                            ccAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTCCCAGCAGGT‑CATAAATAGGCGGTACCGCCATTTTACTGTCGCCCAGGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGc                                                                                                         <  2:607990/249‑1 (MQ=255)

                                                                                                                                                  ccTGCTCAAACATCGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATcaca                                                                                                 <  1:210432/251‑1 (MQ=255)

                                                                                                                                                  ccTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTGGCTGATCGGCACATCGCAGTTTGCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCAATGTGGAACAGGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATcaca                                                                                                 <  1:324267/251‑1 (MQ=255)

                                                                                                                                                     gCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACAcg                                                                                               >  1:581365/1‑250 (MQ=255)

                                                                                                                                                     gCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACAcg                                                                                               >  1:258102/1‑250 (MQ=255)

                                                                                                                                                      atCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGGGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACAcgat                                                                                             >  2:515536/2‑251 (MQ=255)

                                                                                                                                                        cAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACAcgatcg                                                                                           >  2:237017/1‑251 (MQ=255)

                                                                                                                                                        cAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACAcgatc                                                                                            <  1:109649/250‑1 (MQ=255)

                                                                                                                                                         aaaCAACAGTACGTAGCGGTTAACGGGGGA‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGACGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGGGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATAGCGCATTTCACGCCAATCACAcgatcg                                                                                           >  2:226874/1‑250 (MQ=255)

                                                                                                                                                           acaacGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACAcgatcgat                                                                                         >  1:659893/1‑250 (MQ=255)

                                                                                                                                                           acaacGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATa                                                                                        <  1:39684/251‑1 (MQ=255)

                                                                                                                                                            aaacGGTCCGTTGCGGTTAACGTGGGT‑CGGCAGCATCTTGCTGATCGGCACATCGCATTTTTCCAGCAGGT‑CATAAATAGGCTGTACCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCGG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATaa                                                                                       <  2:114019/250‑1 (MQ=255)

                                                                                                                                                              cgggTACGTTGCGTTTAACGGGGGT‑CGGCAGCAGCTTGCTTACCGGCACATCGAAGTTTTCCAGCAGGG‑CATAAATAGGCTGTAACGCCTTTTTACGGTCGCCCATGTTGAACACGGTGACGCCGG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATc                                                                                     <  2:568211/249‑1 (MQ=255)

                                                                                                                                                                     gttgcgtttagggtgggt‑ggGCAGCAGTTTGCTGATCGGCCAAACGAAGTTTTCCAGAAGGC‑AATAAATAGGCTGAAACGCCTTTTTACTGCCGCCCAGGGGGAACACGGTGCCGCCAG‑GTTTACCGCCGAGAAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCAc                                                                                <  2:318334/237‑1 (MQ=255)

                                                                                                                                                                     gTTGCGGTTAACGTGGGT‑CGGCCGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGC‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGCGCACACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGCGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGCTAATCGCCACGt                                                                              >  2:613694/1‑251 (MQ=255)

                                                                                                                                                                          ggTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCtttt                                                                         >  2:654360/1‑251 (MQ=255)

                                                                                                                                                                          cccgacttggggT‑CGGCAGAAGTTTGCTGAACGGATCACCGCAGTTTCCCAGCAGGT‑CAAAAATAGGCTGTACCGCCATTTTCCTTCCGCCCATGGGAAACCCGGTGACGCCGG‑GTTTCCCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAAGCGCCACGTCtttt                                                                         <  2:291760/242‑1 (MQ=255)

                                                                                                                                                                           gTTACCGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCttttt                                                                        <  1:533202/251‑1 (MQ=255)

                                                                                                                                                                               aCGTGGGT‑CGGAAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGTT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACCCGGTGACGCCAG‑GTTTACCGCCGAGCAACCCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCAc                                                                    <  2:715343/251‑1 (MQ=255)

                                                                                                                                                                                 gTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACgg                                                                  <  1:475494/251‑1 (MQ=255)

                                                                                                                                                                                     gT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAAcc                                                              >  2:748184/1‑251 (MQ=255)

                                                                                                                                                                                         ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGt                                                           >  1:279930/1‑251 (MQ=255)

                                                                                                                                                                                            agcagcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTACCGCCATTTTACTGTCGCCCAGGGGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAAc                                                                                                                                                     <  1:139504/158‑1 (MQ=255)

                                                                                                                                                                                            agcagcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTCCCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAAt                                                        <  1:104496/251‑1 (MQ=255)

                                                                                                                                                                                            agcagcTTGCAGATCTGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCTCCATATTGGCCAGGTGATAAAc                                                                                                                                                     >  2:139504/1‑158 (MQ=255)

                                                                                                                                                                                              cagcTTGCTGCTCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGCTCAGCGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGGCTTTTTcc                                                                      >  2:263527/1‑235 (MQ=255)

                                                                                                                                                                                                  ccgCTGAACGGAACGTCGCAGTTTCCCAGCAGGT‑CATAAATGGGCTGTAACCCCATTTTTCTGTCGCCCAGGTGGACCACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCACCGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCg                                                  <  2:826229/249‑1 (MQ=255)

                                                                                                                                                                                                       gATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGa                                               >  2:401812/1‑249 (MQ=255)

                                                                                                                                                                                                       gATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGa                                               >  1:94418/1‑249 (MQ=255)

                                                                                                                                                                                                             cacaTCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCGTTTGACGGCCGCCCAGGGGGAACCGGGTGACGCCAG‑TTTTACCGCCGAGCAAACCGCCAACGCGGGATTCGGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACAt                                       <  2:160364/251‑1 (MQ=255)

                                                                                                                                                                                                                 cgccgtttttcgtgcagCT‑CATCAAGAGGCTGTACCGCGATTTTCCTGTCGCCCTTGTGGAACACGCGGAGGCCAG‑GTCTACCGGCGAGCAAACCGCCGACGTGGGATTCTGCAGCCATAATAGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATGTCACGCCAATCACGCGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGAACATGATa                                   <  2:413499/238‑1 (MQ=255)

                                                                                                                                                                                                                  cGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATaa                                  <  1:237017/251‑1 (MQ=255)

                                                                                                                                                                                                                   gCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATa                                   >  2:256144/1‑249 (MQ=255)

                                                                                                                                                                                                                   gCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATa                                   >  1:697759/1‑249 (MQ=255)

                                                                                                                                                                                                                    cAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAgc                                >  2:311121/1‑251 (MQ=255)

                                                                                                                                                                                                                     aGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAgcg                               >  2:519809/1‑251 (MQ=255)

                                                                                                                                                                                                                           ccAGCAGGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTc                         >  2:344229/1‑251 (MQ=255)

                                                                                                                                                                                                                           ccAGCAGGT‑AATAAATGGGCTGTACCGCTATTTTACTGTCGCCCATGTGGAACACGGTGACGCCTG‑GTTTACCGCCGAGCAAACCGCGAACGCGGTATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAAGGGTGCGGGAAGGGACATGATAAGCGCCGGTc                         <  1:737934/251‑1 (MQ=255)

                                                                                                                                                                                                                                gggT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGGGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCAt                    <  1:831257/250‑1 (MQ=255)

                                                                                                                                                                                                                                agGT‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCAt                    >  1:542398/1‑251 (MQ=255)

                                                                                                                                                                                                                                   t‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATcc                  >  1:815182/1‑250 (MQ=255)

                                                                                                                                                                                                                                   t‑CATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATcc                  >  1:489612/1‑250 (MQ=255)

                                                                                                                                                                                                                                     aataaagtggCTGTACCGCCTTTTTATTGTCGCCCTTGTGGAACGCGTTCACGCCAG‑GTTTCCCCCCGAGCAAACCGCCAACGCGGTATTCTTCCGCCATATTGGCCAGGTGATAACCGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCa                 <  2:430371/242‑1 (MQ=255)

                                                                                                                                                                                                                                       tAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACTTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCTACGGAACCCGTAATGGTGCGTGAAGGGACATGATAAGCGCCGGTCAGAATCCAGgc              >  1:357344/1‑251 (MQ=255)

                                                                                                                                                                                                                                         aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGgcgc            >  1:404961/1‑251 (MQ=255)

                                                                                                                                                                                                                                             ggCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTTAGCATCCAGGCGCTGAt        >  1:71348/1‑251 (MQ=255)

                                                                                                                                                                                                                                                ggaaaCGCTTTTTTACTGTCCCCCATGTGGAACTGGGTGGCGCAGG‑GTTCACCGCAGAGCAACCCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGcc     <  2:140533/248‑1 (MQ=255)

                                                                                                                                                                                                                                                   aaCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCcttc  >  2:295711/1‑251 (MQ=255)

GCTACCGTTGCCGTCGGAGCTGAGGGTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGT‑CGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGT‑CATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAG‑GTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTC  >  NC_000913/4558660‑4559151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`