Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,990,183	T→A	K60* (AAA→TAA)	yqeK ←	protein YqeK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,990,183	0	T	A	100.0%	256.5 / NA	66	K60* (AAA→TAA)	yqeK	protein YqeK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (30/36);  total (30/36)

TGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTAGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAAT  >  NC_000913/2989946‑2990429                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                       tGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTAGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAAtt                                                                                                                                                                                                                                            >  1:48668/1‑250 (MQ=255)   gTGCAAATCCATGAGTCGAACTCGACATTCATCTTAGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAAc                                                                                                                                                                                                                                         <  1:709695/251‑1 (MQ=255)           ccATGAGTCGAACTCGACATTCATCTTAGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACat                                                                                                                                                                                                                                 <  1:286396/251‑1 (MQ=255)            cATGAGTCGAACTCGACATTCATCTTAGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACata                                                                                                                                                                                                                                <  1:118458/251‑1 (MQ=255)                aGTCGAACTCGACATTCATCTTAGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCtt                                                                                                                                                                                                                            >  1:826743/1‑251 (MQ=255)                             aTTCATCTTAGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTcc                                                                                                                                                                                                               <  2:713734/251‑1 (MQ=255)                                 aTCTTAGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGACAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCatg                                                                                                                                                                                                            >  2:211365/1‑250 (MQ=255)                                        cTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATgttgtt                                                                                                                                                                                                    >  1:65218/1‑251 (MQ=255)                                                         cGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATtctc                                                                                                                                                                                   >  1:695089/1‑251 (MQ=255)                                                           tACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATtctc                                                                                                                                                                                   >  1:184524/1‑249 (MQ=255)                                                            aCAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGc                                                                                                                                                                                <  1:536613/251‑1 (MQ=255)                                                            aCAGGCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGc                                                                                                                                                                                <  2:826743/251‑1 (MQ=255)                                                               gTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCAtt                                                                                                                                                                             >  2:286182/1‑251 (MQ=255)                                                                       aaaCAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAAtt                                                                                                                                                                     <  2:562461/251‑1 (MQ=255)                                                                        aaCAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAAttt                                                                                                                                                                    <  2:438332/251‑1 (MQ=255)                                                                         aCAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACTTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGATATATAACTGTTTTTAACTTTACTCTTTACGTTGGCTTAATTGACAGAATTAACATTCACATATCTTGAAGTTAAGGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAAtttt                                                                                                                                                                   <  1:276724/251‑1 (MQ=255)                                                                           aaTACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCGGCATTAAGGAAtttttt                                                                                                                                                                 <  1:578700/251‑1 (MQ=255)                                                                                 gAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACgg                                                                                                                                                           >  2:150620/1‑251 (MQ=255)                                                                                               aaGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCt                                                                                                                                             >  1:852645/1‑251 (MQ=255)                                                                                               aaGAAACAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTc                                                                                                                                              <  2:541169/250‑1 (MQ=255)                                                                                                  aacatgtttacccgttGAAACAATAGTGGAACCGACTAACGTTTCTTTAAAAGATTTGAGTTCATCATCTGCCAAACTAAACTCATCATTGACAGATCGTGTGATTTAACTGTTTTTAACTTTACTCTGTAAGTTGACTTAATTGACCGAATTAACATTCACTTCTCCTGAATTTAATGACCTTGCTGTTGTTTGCGTGAGAACATTCTCAGCATTAATGACTTCTTTTAATGTAATCCTTGGTTTCtctt                                                                                                                                          >  2:179879/16‑248 (MQ=255)                                                                                                   aCCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCttttt                                                                                                                                         >  2:348348/1‑251 (MQ=255)                                                                                                         ttACTCAGTGAATCAATAGAGGAAAGTACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAGACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTTGTTTCTTTTTCGAGt                                                                                                                                    <  1:146606/250‑1 (MQ=255)                                                                                                              cAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCTTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAAt                                                                                                                              <  2:840931/251‑1 (MQ=255)                                                                                                              cAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAAt                                                                                                                              <  2:57819/251‑1 (MQ=255)                                                                                                                gTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAAttt                                                                                                                            >  1:489291/1‑251 (MQ=255)                                                                                                                  gAATCAATAGAGGAAAGTACTAACGTTTCTTTAAAAGAAGTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTTCTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTAc                                                                                                                          <  2:431613/251‑1 (MQ=255)                                                                                                                   aaTCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTcac                                                                                                                                                                                                                                   <  2:849883/145‑1 (MQ=255)                                                                                                                   aaTCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTcac                                                                                                                                                                                                                                   >  1:849883/1‑145 (MQ=255)                                                                                                                           gagGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATg                                                                                                                 <  1:412154/251‑1 (MQ=255)                                                                                                                                acggACTAACGTTTCTTTAAAAGATTTGATTTCATCATCTGTTACACTAAAATCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTAAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTAGAGTCCGACTTTCCAACATCATGCatat                                                                                                            >  2:612468/3‑251 (MQ=255)                                                                                                                                aaGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCatat                                                                                                            >  2:529000/1‑251 (MQ=255)                                                                                                                                 aGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTATACTCATCATTGACAGATCTTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGCATTAACATTCACATATCTTg                                                                                                                                                                                                                           >  2:609979/1‑139 (MQ=255)                                                                                                                                 aGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTGGACTTAATTGACAGAATTAACATTCACATATCTTg                                                                                                                                                                                                                           <  1:609979/139‑1 (MQ=255)                                                                                                                                  ggACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCatat                                                                                                            >  1:19761/1‑249 (MQ=255)                                                                                                                                          gTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTAACATATCGTGAGATATAACTGTTTTTAACTTTACTCTTTTCGTTGACTTAATTGACAGAAGTAACATTCACATATCTTTAATTTAATGTCCATGATGTTTTTTGCGTGAGAACATTCGCAGCATTAAGGAATTTTGTTAACGGAATGCTTTGTTTCTTTTTCGAGTCCGAATTTTGAATATCATGCAGAGTGACCATTTc                                                                                                  <  2:664388/251‑1 (MQ=255)                                                                                                                                                ttAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATg                                                                                               <  1:696878/248‑1 (MQ=255)                                                                                                                                                   aaaGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTg                                                                                         <  1:576020/251‑1 (MQ=255)                                                                                                                                                      gAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGACTCTGCg                                                                                       >  2:700637/1‑250 (MQ=255)                                                                                                                                                       aaTTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGaa                                                                                     <  2:566836/251‑1 (MQ=255)                                                                                                                                                          tGATTTCATCATCTGGTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAAt                                                                                  >  1:522372/1‑251 (MQ=255)                                                                                                                                                             tttCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGCGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGCAAATTc                                                                                >  1:446851/1‑250 (MQ=255)                                                                                                                                                                           aaactaaactCATCATTTACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGtat                                                                  >  1:202223/1‑250 (MQ=255)                                                                                                                                                                             actaaactCATCATTTACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACTGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATGGTATAcc                                                               <  2:695089/251‑1 (MQ=255)                                                                                                                                                                             actaaactCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACa                                                                                                                         >  2:394579/1‑193 (MQ=255)                                                                                                                                                                             actaaactCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACa                                                                                                                         <  1:394579/193‑1 (MQ=255)                                                                                                                                                                                         atTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTAc                                                   >  2:278649/1‑251 (MQ=255)                                                                                                                                                                                           tGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCAAGTCAGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCg                                                 >  2:203110/1‑251 (MQ=255)                                                                                                                                                                                               aGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGAc                                             <  1:134555/251‑1 (MQ=255)                                                                                                                                                                                                gATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACt                                            <  2:48668/251‑1 (MQ=255)                                                                                                                                                                                                    gTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCg                                                                                                                                       <  2:307292/156‑1 (MQ=255)                                                                                                                                                                                                    gTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCg                                                                                                                                       >  1:307292/1‑156 (MQ=255)                                                                                                                                                                                                    gTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGAt                                        >  1:207015/1‑251 (MQ=255)                                                                                                                                                                                                    gTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGAt                                        <  2:19761/251‑1 (MQ=255)                                                                                                                                                                                                    gTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGAt                                        <  1:106689/251‑1 (MQ=255)                                                                                                                                                                                                     tGAGATATAACTGTTTTTAAATTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATg                                       >  2:733236/1‑251 (MQ=255)                                                                                                                                                                                                      gagaTATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATg                                       <  1:407162/250‑1 (MQ=255)                                                                                                                                                                                                       agaTATAACTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGtt                                     >  2:680768/1‑251 (MQ=255)                                                                                                                                                                                                              aCTGTTTTTAACTTTACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTTCTGTTc                              <  1:800636/251‑1 (MQ=255)                                                                                                                                                                                                                           ttACTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCt                   <  1:194972/249‑1 (MQ=255)                                                                                                                                                                                                                             aCTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTcc                <  1:636970/250‑1 (MQ=255)                                                                                                                                                                                                                             aCTCTTTACGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTcc                <  1:278649/250‑1 (MQ=255)                                                                                                                                                                                                                                ctTTACGTTGACTTAAATGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATTTTACTGTTCAATAATCCCCTTCCAt              <  2:805193/249‑1 (MQ=255)                                                                                                                                                                                                                                     cGTTGACTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCGCATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACt       <  2:636839/251‑1 (MQ=255)                                                                                                                                                                                                                                      gTTGACTTAATTGACAGAATTAACATTCACATATCTTGACTTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAGTCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACt       >  2:692611/1‑250 (MQ=255)                                                                                                                                                                                                                                          aCTTAATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATACTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAAt  >  1:611936/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                       TGGTGCAAATCCATGAGTCGAACTCGACATTCATCTTAGCTCGATTATTATTCCTTCGTACAGTCGATGTAAACAATACAGAAAGAGGATTATTAAGAACCAGTTTACTCAGTGAATCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGTCCATTGTATACCTCACATTTTTACCGTGACTCGATGTTACTGTTCAATAATCACCTTCCATCAATACTAAAAT  >  NC_000913/2989946‑2990429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑