Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,337,394	A→G	S522G (AGC→GGC)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,337,394	0	A	G	98.9%	336.0 / NA	93	S522G (AGC→GGC)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (43/49);  total (43/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

ACCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCCAC  >  NC_000913/1337149‑1337638                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      aCCCAAGTGTGCTGATGGCCGCATGCTTGCTGTAGAAAAAAGCCTTAGCACTGGGCCCCAAGCGGCAACCATGGGGCAAATCGTCTCTGGCACCGTGTTTGAAAGTCGTTTCTGATTATCTGGCAAAAGCTAATCTGTCACCGTATCTCGCCGAACTGTGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGTG‑CCGCTGCCCGATCCTATCGAAACGGCAATTCAAAAAGG‑CGAt                                                                                                                                                                                                                                                 <  2:778619/251‑1 (MQ=255)  cccAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGAtt                                                                                                                                                                                                                                                <  1:167132/251‑1 (MQ=255)  cccAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGAtt                                                                                                                                                                                                                                                <  1:714762/251‑1 (MQ=255)     aaGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTa                                                                                                                                                                                                                                              >  1:845082/1‑250 (MQ=255)      aGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAAc                                                                                                                                                                                                                                            <  1:773573/251‑1 (MQ=255)              gATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTcggtg                                                                                                                                                                                                                                    <  1:267881/251‑1 (MQ=255)               aTGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTGCGAAAGTCGTTGCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGGACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGAGCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTcggtgc                                                                                                                                                                                                                                   <  1:688136/251‑1 (MQ=255)                 ggactgaGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGTCACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGACTTACCCGTcggtgcgg                                                                                                                                                                                                                                 <  2:176553/245‑1 (MQ=255)                 ggCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTcggtgcg                                                                                                                                                                                                                                  <  1:852492/250‑1 (MQ=255)                        ggCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTc                                                                                                                                                                                                                          <  2:104819/251‑1 (MQ=255)                                 gCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCg                                                                                                                                                                                                                 <  1:782667/251‑1 (MQ=255)                                   gAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGt                                                                                                                                                                                                                >  1:686989/1‑250 (MQ=255)                                    aaaaaaGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAGACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTa                                                                                                                                                                                                               >  1:376897/1‑250 (MQ=255)                                       aaaGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCTTTTCTGATTATCTGGCAAAAGCGAAACTGACCCCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACtt                                                                                                                                                                                                           <  1:1033/251‑1 (MQ=255)                                             gTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAgg                                                                                                                                                                                                     <  1:552069/251‑1 (MQ=255)                                                         cTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTatccatcc                                                                                                                                                                                         >  2:386031/1‑251 (MQ=255)                                                                           gTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActg                                                                                                                                                                       <  1:270966/251‑1 (MQ=255)                                                                             cAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCg                                                                                                                                                                                        <  1:87064/232‑1 (MQ=255)                                                                             cAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCg                                                                                                                                                                                        >  2:87064/1‑232 (MQ=255)                                                                                     cGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATGTGGCAAAAGCGAAACTGACCCCGTATCGCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTTTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATGTAACCGTCGGTGCGGTGCTGTCCGGCAGCCGTAACTTTGAAGGCCGTATCCAACCGCGGGTTAAAACTAACTGGTTGGCCTc                                                                                                                                                              <  2:218599/250‑1 (MQ=255)                                                                                      gCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGACTcgc                                                                                                                                                            >  2:30855/1‑251 (MQ=255)                                                                                          gCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTcgccgcc                                                                                                                                                        <  1:170961/251‑1 (MQ=255)                                                                                              cGGGTTCGAAAGGCGTTTCTGATTATCTGGCAAAAGCGAAACTGACGCCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCGGTG‑CCGCTGCCCGATCCTACCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCGGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCtg                                                                                                                                                    <  2:358617/251‑1 (MQ=255)                                                                                                ggTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGACGGCCGTATCCATCCGCTGGTTAAAACTAACTGGATGGCCTCGCCGCCGCtggg                                                                                                                                                  >  2:793174/1‑250 (MQ=255)                                                                                                ggTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCtggt                                                                                                                                                  <  1:457424/251‑1 (MQ=255)                                                                                                ggTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCtggt                                                                                                                                                  >  1:632453/1‑251 (MQ=255)                                                                                                        aGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCCATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGTAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTgtct                                                                                                                                          >  2:779921/1‑248 (MQ=255)                                                                                                                  gATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGGTCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCGTATGCGCTgg                                                                                                                                 <  2:288385/250‑1 (MQ=255)                                                                                                                  gATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTACAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGACTATGCGCTGGc                                                                                                                                >  2:820288/1‑251 (MQ=255)                                                                                                                  gATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTTGTGGTTGCCTATGCGCTGGc                                                                                                                                <  2:686989/251‑1 (MQ=255)                                                                                                                  gATTATCTGGCAAAAGCGAAACTGACACCGTATCCCGACGAACTGGGGTTTAACCTTGGGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCt                                                                                                                                   <  2:703242/248‑1 (MQ=255)                                                                                                                    ttATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCgg                                                                                                                              >  1:108344/1‑251 (MQ=255)                                                                                                                    ttATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGCG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGGAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCgg                                                                                                                              >  1:296979/1‑251 (MQ=255)                                                                                                                          ggCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTTCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAGCCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGaaa                                                                                                                          <  1:655607/249‑1 (MQ=255)                                                                                                                           gCAAAAGCGAAACTGACCCCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAatat                                                                                                                       >  1:566036/1‑251 (MQ=255)                                                                                                                            cAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATg                                                                                                                      <  2:57356/251‑1 (MQ=255)                                                                                                                              aaaGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATgcccatttagcgaaatcgat                                                                                                                    >  1:336005/1‑233 (MQ=255)                                                                                                                                 gCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAatat                                                                                                                 <  1:757198/251‑1 (MQ=255)                                                                                                                                 gCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAatat                                                                                                                 <  1:386031/251‑1 (MQ=255)                                                                                                                                  cGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATc                                                                                                                >  1:728843/1‑251 (MQ=255)                                                                                                                                  cGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTATACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATc                                                                                                                >  1:56463/1‑251 (MQ=255)                                                                                                                                    aaaCTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTCTTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGTTTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCa                                                                                                               >  1:324977/1‑250 (MQ=255)                                                                                                                                    aaaCTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCa                                                                                                               >  1:319268/1‑250 (MQ=255)                                                                                                                                     aaCTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAAc                                                                                                             >  2:792104/1‑251 (MQ=255)                                                                                                                                         gACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTgg                                                                                                         >  1:660125/1‑251 (MQ=255)                                                                                                                                         gACACCGTATCACGACGAACTGGGGTTTAACCTTGTGGG‑AATGGGTTGTACCCCCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCTAAACGGCAATCAAAAAAGG‑GGATTTAACCGTCGGTGCGGTGCTTTCCGGCAACCGTACCTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTg                                                                                                          <  2:367719/250‑1 (MQ=255)                                                                                                                                          acacCGTATCTCGGCGAACTGGGGTTTAACCGTGTGGG‑ATGCGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGc                                                                                                        <  2:544015/251‑1 (MQ=255)                                                                                                                                          acacCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCCACCTGGc                                                                                                        >  1:650822/1‑251 (MQ=255)                                                                                                                                            acCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAACATGAATATCAACCCGGCtt                                                                                                      >  2:583492/1‑251 (MQ=255)                                                                                                                                              cGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCt                                                                                                    <  2:329615/251‑1 (MQ=255)                                                                                                                                                 atcgtgacgaACGGGGGTTTAGCCTTGTGAG‑ATACGGTTGTACCACATGTATTGTTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGTTGCGGTGCTGTCCGTCAACCGTAACTTTGAAGGCCGTAGCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGTCGCCGCTGGGGGTGGCCTATGCGCTAGCGGGAAAGATGAATATCAACCTGGCTTCTGa                                                                                                  <  2:331405/245‑1 (MQ=255)                                                                                                                                                 aTCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGACACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCACACTGGCTTCTGa                                                                                                  >  2:249525/1‑250 (MQ=255)                                                                                                                                                 aTCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGa                                                                                                  <  1:372258/250‑1 (MQ=255)                                                                                                                                                  tctcGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTCTCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGGGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAACTATGAATATCAACCTGGCTTCTGatc                                                                                                >  1:30515/1‑249 (MQ=255)                                                                                                                                                      gacgacCTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAACAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACAGTAACTTTGAAGGCCGAATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAACCTAt                                                                                            >  1:269495/1‑251 (MQ=255)                                                                                                                                                       acgaACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATc                                                                                           <  2:447220/251‑1 (MQ=255)                                                                                                                                                        cgaACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCg                                                                                          >  2:251932/1‑251 (MQ=255)                                                                                                                                                        cgaACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCg                                                                                          <  2:266079/251‑1 (MQ=255)                                                                                                                                                          accTGGGTTTTAACCTTGTGGG‑CAACGGTTGTACGTACTGTATTGGTAACTCGGGG‑CCGCTGCCCGATCCTATCGTAAGGGTAATCAAAAAAGG‑CTATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGTTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCATCCTGGCGTCTGAGCCGATCGGc                                                                                        <  2:601297/249‑1 (MQ=255)                                                                                                                                                          aaCTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAAAATCAACCTGGCTTCTTAGCCTATCggg                                                                                        >  2:760225/1‑250 (MQ=255)                                                                                                                                                           aCTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGAGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTTGTGGTTGCCTATGCGCTGGCGGGAGATATGAATATCAACCTGGATTCTGAGCCCATCGGcc                                                                                       >  2:11797/1‑251 (MQ=255)                                                                                                                                                                ggTTTATCCTTGTGGGCAT‑CGGTTGGCCCAACTGTATAGGTAACTCGGTG‑CCGCTGTCCGATCCGATCGAAACTGCAATCAAAAAAGT‑CGATTTCACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTAGCCATCCTCTGGTTAAAACTACCTGGCTGGCCTCGCCGCCGCTGGTGGTTGTCTATGCGCTGGCGGGAAATATGAATATCATCCTGGCTTCTGAGCCTATCGGCCATGAt                                                                                  <  2:572465/251‑1 (MQ=255)                                                                                                                                                                    tAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGc                                                                               <  2:772944/250‑1 (MQ=255)                                                                                                                                                                     aaCCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACGCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGGGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCa                                                                              <  2:728843/250‑1 (MQ=255)                                                                                                                                                                      aCCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCaaa                                                                            <  1:42243/251‑1 (MQ=255)                                                                                                                                                                      aCCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCaaa                                                                            <  1:30855/251‑1 (MQ=255)                                                                                                                                                                          tgtgGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAgg                                                                          <  2:426553/249‑1 (MQ=255)                                                                                                                                                                          tgtgGG‑ATACGGTTGTACCACCTGTATTGGTAACCCTGGC‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTGCTGAGCCTATCGGCCATGATCGCAAAGGc                                                                         <  1:658356/250‑1 (MQ=255)                                                                                                                                                                           gtgGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGa                                                                       <  2:58543/251‑1 (MQ=255)                                                                                                                                                                           gtgGG‑ATACGGTTGTACCACCGGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGAAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCg                                                                        <  2:68804/250‑1 (MQ=255)                                                                                                                                                                            tgGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATAATCGCAAAAGCGAt                                                                      >  2:722765/1‑251 (MQ=255)                                                                                                                                                                               g‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGaaccc                                                                   >  2:839245/1‑247 (MQ=255)                                                                                                                                                                                   gcGGTTGTACCACCGGTATTGTTAACTCTGGG‑CCGCTGCCCGTTCCTATAGAAACGGTAAGCAACAAAGG‑CGTTTTAACCGTCGGTGAGGTGCTGTCCGGCAACCGTAGCTTTGAAGGCCGTATCCATCCGCTGTTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCCGAGCCTATCGGCCATGATCTCAAAGGCGATCCGGtt                                                                <  2:562859/250‑1 (MQ=255)                                                                                                                                                                                   aCGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCAGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATAAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATACGGtt                                                                >  1:676562/1‑251 (MQ=255)                                                                                                                                                                                     ggTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGCAATATGAATATCAACCCGGCTTCTCAGCCTATCGGCCATGGTCGAAAAGGCGATACGGtttt                                                              >  2:350085/1‑250 (MQ=255)                                                                                                                                                                                        ggtcccacgTGTATTGGTATC‑CTGGGGCGGCTCCCCGATCCAATCGAAACGGCAAT‑AAAAAAAGTGGATTTAACCGTCGGTGCGGTCCTGCCCGGCAACCG‑AACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTg                                                          <  2:845082/247‑1 (MQ=255)                                                                                                                                                                                                    atgggTAACCCTGGG‑CCGCCCCCAGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCAATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCGATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGACAGATATCTg                                               <  2:280493/248‑1 (MQ=255)                                                                                                                                                                                                       ggTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGcc                                            <  1:792104/251‑1 (MQ=255)                                                                                                                                                                                                       ggTAACCCTTGG‑CCGCTTCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGGCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACCGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCACGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGcc                                            <  1:820288/251‑1 (MQ=255)                                                                                                                                                                                                          aaCTCTGGG‑CCGCAGCCCGATACTATCGAAACGGCAATCAAAAAAGG‑CGATTTAAACGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCACGTACGCAAAGGCGATCCGGTTTATCTGAAAACTATATGGCCATc                                         >  2:214476/1‑251 (MQ=255)                                                                                                                                                                                                                 gg‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGcaca                                   >  1:817659/1‑250 (MQ=255)                                                                                                                                                                                                                    ccGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGcaca                                   >  1:654796/1‑248 (MQ=255)                                                                                                                                                                                                                     cGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGa                                >  1:98353/1‑250 (MQ=255)                                                                                                                                                                                                                          cccGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGc                          >  1:421845/1‑251 (MQ=255)                                                                                                                                                                                                                            cGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGccc                        >  1:808325/1‑251 (MQ=255)                                                                                                                                                                                                                            cGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGccc                        >  1:269734/1‑251 (MQ=255)                                                                                                                                                                                                                             gATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATTTGGCCATCGGCACCAGAAATTGCCCg                       >  2:444080/1‑251 (MQ=255)                                                                                                                                                                                                                             gATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCg                       >  1:630749/1‑251 (MQ=255)                                                                                                                                                                                                                                 cTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTCGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCg                   >  1:848898/1‑251 (MQ=255)                                                                                                                                                                                                                                       aaaCGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGGTCCGGTTTATCTGAAAGATATCTGGCCCTCGGCACAAGGAATTGACCGGGCGGTAGa              >  2:290875/1‑250 (MQ=255)                                                                                                                                                                                                                                          cGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACaa          <  1:583492/251‑1 (MQ=255)                                                                                                                                                                                                                                           ggCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAg         <  1:839245/251‑1 (MQ=255)                                                                                                                                                                                                                                           ggCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCAACGGCACAAGAAATTGCCCGTGCGGTAGAACAAg         <  1:722765/251‑1 (MQ=255)                                                                                                                                                                                                                                                  aaaaaagG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACGAGTCTCcac  <  1:808594/251‑1 (MQ=255)                                                                                                                                                                                                                                                  aaaaaagG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAAACTGGCTTCAGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCCTCGGCACAAGGAATTGCCCGGGCGGGAGAACAAGTCCCcac  >  2:832106/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      ACCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGG‑ATACGGTTGTACCACCTGTATTGGTAACTCTGGG‑CCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCCAC  >  NC_000913/1337149‑1337638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑