Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	536,549	T→C	intergenic (+63/‑37)	hyi → / → glxR	hydroxypyruvate isomerase/tartronate semialdehyde reductase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	536,549	0	T	C	100.0%	274.9 / NA	75	intergenic (+63/‑37)	hyi/glxR	hydroxypyruvate isomerase/tartronate semialdehyde reductase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/35);  total (40/35)

ATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTAACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCTGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTATGGGTACACCGATGGCCATTAATCTGGCGCGTGCCGGTCATCAATTACATGTCACGACCATTGGACCGGTTGCTGATGAATTACTGTCACTGGGTGCCGTCAGTGTTGAAACTGCTCGCCAGGTAACGGAAGCATCGGACATCATTTTTATTATGGTGCCGGACACACCT  >  NC_000913/536307‑536786                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              aTTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTc                                                                                                                                                                                                                                         >  1:336409/1‑249 (MQ=255)       cACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGaaaa                                                                                                                                                                                                                                  <  1:426144/250‑1 (MQ=255)       cACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATGCAGGaaaa                                                                                                                                                                                                                                  <  1:562562/250‑1 (MQ=255)            gCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGaa                                                                                                                                                                                                                             >  1:527250/1‑250 (MQ=255)                     cGATAATCCGCATCTCGGCGAACCTGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAAATCTCACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGATTTTTTAGGCCGCTAAGTTGTCAAGGTATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAaga                                                                                                                                                                                                                   <  2:188287/251‑1 (MQ=255)                     cGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTaaa                                                                                                                                                                                                                      >  1:769993/1‑248 (MQ=255)                      gATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTCAAag                                                                                                                                                                                                                    >  2:185477/1‑249 (MQ=255)                       aTAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCGGACTACAACGGTTGGGTTGGGTGTTAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAgg                                                                                                                                                                                                                 <  1:388856/251‑1 (MQ=255)                          aTCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGGACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGACAAGCGAAATTTAAAAGAGGtt                                                                                                                                                                                                               >  2:730989/1‑250 (MQ=255)                            ccGCATCGCGGCGAACCGGGAACCGGAGAACTTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAAt                                                                                                                                                                                                            >  2:726172/1‑251 (MQ=255)                                    cggcgAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAAc                                                                                                                                                                                                    >  1:338747/1‑251 (MQ=255)                                    cggcgAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAAc                                                                                                                                                                                                    >  1:47152/1‑251 (MQ=255)                                     ggcgAACCGGGAACCGGAGCAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGACGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACt                                                                                                                                                                                                   >  2:506576/1‑251 (MQ=255)                                        gaaccgggaaccggAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTggg                                                                                                                                                                                                >  2:749351/1‑251 (MQ=255)                                        gaaccgggaaccggAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTggg                                                                                                                                                                                                >  1:114144/1‑251 (MQ=255)                                             gggaaccggAGAAATTAAGTATGATTATCTCGTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGTGTTTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGAGTTGACCGAATTCAGGTAAAGCGAAATTTAAAAGAGGTTAATTATGAAGCTgggtttgt                                                                                                                                                                                           <  2:529360/251‑6 (MQ=255)                                              ggaaccggAGAAATTAATTATGATTATCGCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTAt                                                                                                                                                                                          <  1:416402/251‑1 (MQ=255)                                              ggaaccggAGAAATTAAATATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTTGGTGTGAATATAAACCACAAACCACCACGGAAGCCGGGTTACGCTGGATGGATCCGTACCGGTAAAACGTGACGCTATTCAGACAATGCTTTGTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGGTAATTATTAAAATGGGAGTTAt                                                                                                                                                                                          <  2:616964/251‑1 (MQ=255)                                                aaccggAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTTCAACGGTTGGGTTGGGTGTGAATATAAACCCCAATCCACCACGGAATCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTg                                                                                                                                                                                        <  1:728619/251‑1 (MQ=255)                                                aaccggAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTg                                                                                                                                                                                        >  2:405466/1‑251 (MQ=255)                                                 accggAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTgg                                                                                                                                                                                       <  2:20715/251‑1 (MQ=255)                                                         aattaattATTGTTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCa                                                                                                                                                                               <  2:415460/251‑1 (MQ=255)                                                          attaattaTGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCAACACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCAt                                                                                                                                                                              >  2:564540/1‑251 (MQ=255)                                                            taattaTGATTATCTCTTTAAGGAAATCGAAAATTCTGACTACAACGGTTGGGATGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTAGGCAGGGGATCGAGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCAtt                                                                                                                                                                             >  2:593041/1‑250 (MQ=255)                                                              aTTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgt                                                                                                                                                                          <  1:280099‑M1/251‑3 (MQ=255)                                                                  tGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtc                                                                                                                                                                      >  1:792894‑M1/1‑245 (MQ=255)                                                                           ctTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcatt                                                                                                                                                             >  2:700446‑M1/1‑236 (MQ=255)                                                                            tttAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGAATTAgtagtcgtaggcatt                                                                                                                                                             >  2:74943‑M1/1‑235 (MQ=255)                                                                                aGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagac                                                                                                                                                         >  1:337697‑M1/1‑231 (MQ=255)                                                                                 ggTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAAtt                                                                                                                                                                                                           >  1:179699/1‑199 (MQ=255)                                                                                 ggTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAAtt                                                                                                                                                                                                           <  2:179699/199‑1 (MQ=255)                                                                                  gTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggccttagacatt                                                                                                                                                      >  2:456226‑M1/1‑229 (MQ=255)                                                                                       cGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGCATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgc                                                                                                                                                 >  2:382763‑M1/1‑224 (MQ=255)                                                                                            aTTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgat                                                                                                                                            <  1:99900‑M1/251‑33 (MQ=255)                                                                                              tCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgc                                                                                                                                          >  1:423042‑M1/1‑217 (MQ=255)                                                                                                 gACTACAACGGTTGGGTTGGGTGTGAATATAACCCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgc                                                                                                                                          <  1:223535‑M1/248‑35 (MQ=255)                                                                                                  aCTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacg                                                                                                                                      <  2:662630‑M1/251‑39 (MQ=255)                                                                                                     aCAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGGTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgct                                                                                                                                    <  1:456226‑M1/250‑41 (MQ=255)                                                                                                          ggttgggttgGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgc                                                                                                                              <  1:45178‑M1/251‑47 (MQ=255)                                                                                                                   gggtGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatca                                                                                                                     <  1:589707‑M1/251‑56 (MQ=255)                                                                                                                    ggtGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatc                                                                                                                      >  1:456794‑M1/1‑195 (MQ=255)                                                                                                                     gtgtGAATATAAACACCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAACACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAACGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGAATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcagg                                                                                                                   >  2:26798‑M1/1‑194 (MQ=255)                                                                                                                           atatAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgta                                                                                                                                                                   >  1:690420‑M1/1‑188 (MQ=255)                                                                                                                           atatAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgta                                                                                                                                                                   <  2:690420‑M1/197‑10 (MQ=255)                                                                                                                                 aCCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggg                                                                                                       >  2:110765‑M1/1‑182 (MQ=255)                                                                                                                                      aaaCCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGt                                                                                                                                                                                                                >  2:201894/1‑141 (MQ=255)                                                                                                                                      aaaCCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGt                                                                                                                                                                                                                <  1:201894/141‑1 (MQ=255)                                                                                                                                                 gAAGCCGGGTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTGTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaatt                                                                                       <  2:430624‑M1/251‑86 (MQ=255)                                                                                                                                                  aaGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggc                                                                                           <  1:743763‑M1/246‑82 (MQ=255)                                                                                                                                                  aaGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggc                                                                                           >  2:743763‑M1/1‑165 (MQ=255)                                                                                                                                                      cGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGCAATTTAAAAGAGGTTAATTATGAAACTGGGATTTACTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtg                                                                                   >  2:538255‑M1/1‑161 (MQ=255)                                                                                                                                                       ggTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgc                                                                                  >  1:652836‑M1/1‑160 (MQ=255)                                                                                                                                                            aCGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaat                                                                             <  2:336409‑M1/250‑96 (MQ=255)                                                                                                                                                                  gatggatCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgat                                                                      <  1:733808‑M1/251‑103 (MQ=255)                                                                                                                                                                     ggatCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcg                                                                   >  1:375171‑M1/1‑146 (MQ=255)                                                                                                                                                                       atCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgat                                                                 >  1:301282‑M1/1‑144 (MQ=255)                                                                                                                                                                           gTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgctt                                                             <  1:526917‑M1/251‑112 (MQ=255)                                                                                                                                                                                        gtgacgcTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatacggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatc                                                >  1:589731‑M1/4‑127 (MQ=255)                                                                                                                                                                                          gacGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatc                                                >  1:256551‑M1/2‑125 (MQ=255)                                                                                                                                                                                              cTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttctcaggcct                                          >  2:839725‑M1/1‑121 (MQ=255)                                                                                                                                                                                               tATTCAGACAAGGCATTTTTAGGCCTCTAAGTTGGCAGGGGATCGAGCTGTCCGAATTCAGGAAAAGCGAAATTTAACAGAGGTTAATTATGAAAATGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgacgcatccggcaattggtgcacaatgcctgatgcgatgcttgatgcatcttatcaggcct                                          >  2:324420‑M1/1‑120 (MQ=255)                                                                                                                                                                                                aTTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctac                                        <  2:522885‑M1/251‑133 (MQ=255)                                                                                                                                                                                                   cAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaat                                     <  1:74390‑M1/251‑136 (MQ=255)                                                                                                                                                                                                    aGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatg                                    <  2:423042‑M1/251‑137 (MQ=255)                                                                                                                                                                                                       cAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggt                                 >  1:252570‑M1/1‑112 (MQ=255)                                                                                                                                                                                                           gCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtacc                              <  1:405466‑M1/250‑143 (MQ=255)                                                                                                                                                                                                                tttAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatc                         <  2:13977‑M1/250‑148 (MQ=255)                                                                                                                                                                                                                    ggCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctaatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtag                    <  1:74943‑M1/251‑153 (MQ=255)                                                                                                                                                                                                                        gCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccg                >  2:140899‑M1/1‑95 (MQ=255)                                                                                                                                                                                                                        gCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaacaggtgccgcatccggcaatgggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggttccggatcggtaggccg                >  2:13980‑M1/1‑95 (MQ=255)                                                                                                                                                                                                                               tGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataag          <  2:769993‑M1/250‑163 (MQ=255)                                                                                                                                                                                                                                ggCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtagtccgtataaggc        <  1:64569‑M1/251‑165 (MQ=255)                                                                                                                                                                                                                                      ggATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttac  <  2:325668‑M1/251‑171 (MQ=255)                                                                                                                                                                                                                                        aTCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctcatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatggg                                  <  1:422585‑M1/217‑139 (MQ=255)                                                                                                                                                                                                                                        aTCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctcatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatggg                                  >  2:422585‑M1/1‑79 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              ATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTAACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCTGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTATGGGTACACCGATGGCCATTAATCTGGCGCGTGCCGGTCATCAATTACATGTCACGACCATTGGACCGGTTGCTGATGAATTACTGTCACTGGGTGCCGTCAGTGTTGAAACTGCTCGCCAGGTAACGGAAGCATCGGACATCATTTTTATTATGGTGCCGGACACACCT  >  NC_000913/536307‑536786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑