| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,004,132 | 0 | G | A | 30.0% | 109.2 / 19.2 | 62 | A16V (GCT→GTT) | rarD | putative transporter RarD |
| Reads supporting (aligned to +/- strand): ref base G (9/33); new base A (18/0); total (29/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.07e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTG‑AAGTACGCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA‑CGCCCTGCCGCG‑TTTGTTTTG‑CATCCATC‑GGGAATACTCATTTTTAATTGGTAACAGCAG‑TTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCA > NC_000913/4003887‑4004380 | ggTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCAAGGAGTAAAACAACTTT‑AAGTACGCTGGCGCTAT‑AACCCAAAAAAAAAAAACGGc > 1:471473/1‑250 (MQ=255) gTGATTGTTCACCGACCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTG‑AAGTACGCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCaa < 2:388940/251‑1 (MQ=255) aTTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGTCCGTAGTAAATCAACTTT‑AAGTACGCCGGCGCTAT‑ACCCCAAAAAAAATAAACGGccaa > 1:73371/1‑246 (MQ=255) aTTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTG‑AAGTACGCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAagag < 2:636685/251‑1 (MQ=255) cACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTG‑AAGTACGCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGt < 2:311100/250‑1 (MQ=255) cACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTG‑AAGTACGCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTa < 1:518647/251‑1 (MQ=255) ccGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTG‑AAGTACGCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAAt < 1:120851/251‑1 (MQ=255) cccAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTG‑AAGTACGCTGGCGCTAT‑ACCCCAAATAAAATAAGCgg < 1:178219/234‑1 (MQ=255) aGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACCGAGTAAATAAAATTT‑AAATAAGCTGGGGCCAT‑ACCCCCAAAAAAATAAACCGCCAGAGGGAGGAATA‑AGCCCTCCcgcgg > 1:830037/1‑250 (MQ=255) gCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTG‑AAGTACGCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA‑CGCCCTGCCGCG‑TTTGTTTTG‑cat < 1:179160/251‑1 (MQ=255) gCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTG‑AAGTACGCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA‑CGCCCTGCCGCG‑TTTGTTTTG‑cat < 1:208457/251‑1 (MQ=255) gCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAAGTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCTCTGGCGGCAAATGCTCATCAGCACCTCCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGTGCACGTAGTAAATCAACTTG‑AAGTACGCTGGCGCTAT‑CCCCCAAATAAAATAAGCGTCAAGAGCGAGTAATA‑CGCCCTGCCGCG‑TTTGTTTTG‑cat < 2:407842/251‑1 (MQ=255) aaTCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGGAGTAAATAAACTTG‑AAGTACGCTGGAGCCAT‑AACCCAAATAAAAAAAACGGCAAGAGCGAGTAATA‑CGCCCTGCCGCG‑GTTGttttttatccctcg > 1:474907/1‑241 (MQ=255) tCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAGATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAGCTTG‑TAGTACGCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA‑CGCCCTGCCGCG‑TTTGTTTTG‑CATCCAGC‑gg < 1:727114/250‑1 (MQ=255) aGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGGACGGAGGAAAACAACTTT‑AAGTACGCCTGCGCTAT‑ACCCCCAATAAAAAAAACAGCAAAAACGGGGAATA‑AACCCCGCCGCG‑CTTGTTTTT‑TATCCATC‑ggggg > 2:284221/1‑249 (MQ=255) gCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGGCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGGACGTAGTAAAGCAGCTTG‑AGGTACGCTGGCGCTAT‑TCCCCAAATAAAATAAGCGGGAAGAGCGAGTAATA‑CGCCCTGCCGCG‑TTTGTTTTG‑CATCCAGC‑GGGAAt < 1:703256/251‑1 (MQ=255) ggCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCACCTCCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTG‑AAGTACGCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA‑CGCCCTGCCGCG‑TTTGTTTTG‑CATCCATC‑GGGAATACTc < 2:830037/251‑1 (MQ=255) ggCAGAGACCGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTCAAAAAACTTG‑AGATACGCTGGGCCCAT‑ACCCCAAAAAAAATAATCGGCCAGCGGCAGGAATA‑AGCCCTGCCGCGGTTTTTTTTG‑C‑TCCATC‑ggggagaacc > 1:568795/1‑244 (MQ=255) agagACTTCCAGCATAAAAATTTTCTGTGGCGGCTGAATCAGCGTTTTTAAATAGGACCACGGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTG‑AAGTACGCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA‑CGCCCTGCCGCG‑TTTGTTTTG‑CATCCATC‑GGGAATACTCAtt < 2:677999/251‑1 (MQ=255) agagACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTG‑AAGTACGCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA‑CGCCCTGCCGCG‑TTTGTTTTG‑CATCCATC‑GGGAATACTCAtt < 1:189510/251‑1 (MQ=255) gaCTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCAAGGTGTAAAACAACTTG‑AAGTACGCTGGCGCCAT‑ACCCCCAAAAAAAAAAAAGGCAAAAGAGGGGAATA‑CGCCCCTCCGCG‑TTTGTTTTT‑CCTCCAAC‑GGGGATACACAttttt > 2:41748/1‑251 (MQ=255) aTAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCCCGGAGTAAATCAACCTG‑GAGTAAGCGGGCGCCAT‑AACCCAAATAAAATAAGCGGCAAAAGCGGGGAAAA‑AGCCCCGCCGCG‑GTTGTTTTG‑CATCCATC‑GGGAATACTCATTTTTTATTTGTAAc > 1:76792/1‑251 (MQ=255) aaaaaTTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGAAGTAAAACAACTTG‑TAGTAAGCTGGGGCTATCACCCCAAA‑AAAATAAAAGGGCACAGTGGGAAATAACTCCCCCCCCGG‑TTTTTTTTG‑‑ATTCATC‑GGGAATACTCATTTTTTATTGGTAAca > 2:154140/1‑250 (MQ=255) gtgGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAATCGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTG‑AAGTACGCTTGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA‑CGCCCTGCCTCG‑TTTGTTTTG‑CATCCATC‑GTGAATACTCATTTTTAATTGGTAACAGCAG‑TTTAc < 1:224153/248‑1 (MQ=255) ggCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTG‑AAGTACGCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA‑CGCCCTGCCGCG‑TTTGTTTTG‑CATCCATC‑GGGAATACTCATTTTTAATTGGTAAcagca < 1:389545/240‑1 (MQ=255) ggCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACCGAGGAAATAAACTTG‑AAGTACGCTTGCGCTCT‑ACCCCCAATAAAAAAAAAGGGAAGAGCGAGGAAAA‑CACCCTGCCGCG‑GTTGTTTTTTCATC‑TTC‑GGGGATACTCATTTTTTAtttgttacaaga > 2:389545/1‑230 (MQ=255) gAATCAGCGTTTTTAAATAGGCCCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTG‑AAGTACGCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA‑CGCCCTGCCGCG‑TTTGTTTTG‑CATCCAGC‑GGGAATACTCATTGTTAATTGGTAACAGCAG‑TTTACCTGCTTTTATGt < 1:638188/251‑1 (MQ=255) aTCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGCAGTAAATCAACTTG‑AAGTAAGCTGGCGCTAT‑ACCCCCAATAAAATAAGAGGGAAGAGGGAGGAATA‑CGCCCTTCCGCG‑GTTGTTTTT‑CATTCATC‑GGGGATACTCATTTTTAATTGGTAACAGCAG‑TTTTCCTGCTTTTATGttt > 1:85577/1‑249 (MQ=255) ttttAAATAGGACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACCGAGTAAATCAACTTT‑AAGTAAGCTGGCGCTAT‑ACCCCCAATAAAAAAAACGGCCAGAGCGAGTAATA‑AGCCCCGCCGCG‑TTTGTCTTG‑CATCCATC‑GGGGATACTCATTTTTAATTGGTAACAGCAG‑GTTACCTGCTTTTATGTCTTCAACCt > 1:842706/1‑250 (MQ=255) aaaTAGGACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTG‑AAGTACGCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA‑CGCCCTGCCGCG‑TTTGTTTTG‑CATCCATC‑GGGAATACTCATTTTTAATTGGTAACAGCAG‑TTTACCTGCTTTTATGTCTTCAACCTACCat < 2:767699/251‑1 (MQ=255) ggACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATAAACTTG‑AAGTAAGCTTGCGCTAT‑ACCCCAAATAAAAAAAACGGCCAGAGCGAGGAATA‑CGCCCTGCCGCG‑TTTGTTTTT‑CATCCATC‑GGGGATACTCCTTTTTAATTTGTAACAGCAG‑TTTACCTTCTTTTTTTTCTTTAAACTACCCTATAgg > 1:709814/1‑251 (MQ=255) aCCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCCCACAGTAAATCAACCTG‑AAACACGCTTGGGCTCA‑ACCCCCAACAAAAAAAAAGGCCAGAGAGAGGAATA‑ATCCCCGCCGCG‑GTTGTTTTG‑TATTCCTC‑CGGGATACTCAATTTTATTTGGTAAAAACAG‑ATTTCCTTCTTTTTTTTCTTCAACCCACCATATTggtt > 2:454881/1‑250 (MQ=255) aCCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTG‑AAGTACGCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA‑CGCCCTGCCGCG‑TTTGTTTTG‑CATCCATC‑GGGAATACTCATTTTTAAGTGGTAACAGCAG‑TTTACCTGCTTTTATGTCTTCAACCTACCATATAggtg < 2:127559/251‑1 (MQ=255) aaaTGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTG‑AAGTACGCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA‑CGccc < 1:336564/146‑1 (MQ=255) aaaTGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAAACAACTTT‑AAGTACGCCTGCGCCAT‑ACCCCCAAAAAAAAAAACGGCAAGAGCGGGTAATA‑CGACCCGCCCCG‑GTTGTTTTT‑CATCCATC‑GGGGATACTCATTTTTAATTTGTAACAGCAG‑TTTTCCTGCTTTTTTTTCTTCAAACTACCCTATAGGTGGAGGGGGCAccg > 1:31890/1‑249 (MQ=255) aaTGCTCATCCGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGGACGGAGTAAATCAACTTGGAAG‑ACGCCGGGGCTTT‑AACCCAAATAAAAAAAACGGCAAGAGCGAGGAATA‑CGCCCTGCCGCG‑GTTGTTTTG‑CATTCATCAGGGAA‑ACTCATTTTTTATTTGTAACAGCCG‑TTTACATGCTTTTTTTTCTTTAACCTACCCTATAAGTGGCGGTGGCACTgg > 1:128873/1‑251 (MQ=255) aaTGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGGAGTAAATCAAATTT‑AAGTAAGCTGGCGCTAT‑ACCCCCAATAAAATAAAAGGCAAAAGCGGGAAATA‑AGCCCCACCGCG‑TTTTTTTTG‑CATCCATC‑GGGGATAATCATTTTTTATTGGGAACAACAG‑TTTTACTGCTTTTTTTTTTTCAAACTTCCCTATACGTGGCGGGGGCACTgg > 1:660406/1‑251 (MQ=255) catcaGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTG‑AAGTACGCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA‑CGCCCTGCCGCG‑TTTGTTTTG‑CATCCATC‑GGGAATACTCATTTTTAATTGGTAACAGCAG‑TTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAat < 1:284221/249‑1 (MQ=255) atcaGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCAAGCAATAAAACAACTTG‑AAGTAAGCTGGCGCCAT‑ACCCCCAAAAAAATAAAAGGCACGAGAGAGTAATA‑CGCCCCGCCGCG‑GTTGTTTTG‑CACTCATC‑GGGAAAACTCATTTTTTATTGGTAACAGCAG‑TTTACATGCTTTTATGTCTTCTACCTACCATATatgtgg‑ggtggggtccgggcaactt > 2:542592/1‑237 (MQ=255) caccacCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGCAATAAATACACTTG‑AAGTACGCTGGCGCTAT‑ACCCCAAAAAAAATAAACGGCCAGAGCGAGGAATA‑CGCCCCGCCGCG‑ATTTTTTTT‑CCTCCATC‑AGGGATACTCCTTTTTTATTTGGAACAACAG‑TTTTCCTGCTTTTTTTTCTTCCACCTACCATATAAGTGGGGGTGGCACCTGCAATATaaaact > 2:5641/1‑248 (MQ=255) ccaccaTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTG‑AAGTACGCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA‑CGCCCTGCCGCG‑TTTGTTTTG‑CATCCATC‑GGGAATACTCATTTTTAATTGGTAACAGCAG‑TTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGt < 1:346781/251‑1 (MQ=255) aCGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTG‑AAGTACGCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA‑CGCCCTGCCGCG‑TTTGTTTTG‑CATCCATC‑GGGAATACTCATTTTTAATTGGTAACAGCAG‑TTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAAt < 1:678380/251‑1 (MQ=255) aCGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTG‑AAGTACGCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA‑CGCCCTGCCGCG‑TTTGTTTTG‑CATCCATC‑GGGAATACTCATTTTTAATTGGTAACAGCAG‑TTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAAt < 1:106442/251‑1 (MQ=255) cGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTG‑AAGTACGCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA‑CGCCCTGCCGCG‑TTTGTCTTG‑CATCCATC‑GGGAATACTCATTTTTAATTGGTAACAGCAG‑TTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAAtt < 2:842706/251‑1 (MQ=255) cGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAAATTG‑AAGGAAGCTGGCGCTAT‑ACCCCAAATAAAAAAAACGGCAAGAGCGAGGAATA‑CGCCCTGCCGCG‑GTTGTTTTT‑CATCCCTC‑GGGAATACTCCTTTTTTATTGGTAACAGCAG‑TTTACCTGCTTTTATTTCTTCAACCTACCATATAGGTGGCGGGGGGACTGGGAATATAAAGCTGTTCTTCATTGTGTAAtt > 1:844531/1‑251 (MQ=255) ccAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAAAGAATCAACTTA‑AAGTACGCTGGGGCTAT‑ACCCCCAATAAAATAACCAGCAAGAGCGAGTAAAA‑CGCCCTGCCGCG‑TTTGTTTTG‑CCTCCATC‑GGGGATAATCATTTTTAATTTGTAAAAGAAG‑GTTACCCCCTTTTTTTTCTTTAACCTAACATATAGGTGGGGGAGGCACTGGCTATATAAAGCtattttttattttttaattta > 1:261375/1‑230 (MQ=255) gTCAGGATTTCATCGGCGGGCACGTAGTAAAAAAACTTG‑AAGTACGCTGGCGCTAT‑ACCCCCAATAAAAAAAACGGCAAGAGCGAGGAATA‑CGCCCTGCCGCG‑TTTGTTTTT‑CATCCATC‑GGGAAAAATCATTTTTTATTTGTAACAACAGGTTTAC‑TTCTTTTATTTTTTCAACCTACCCTATAGGTGGCGGGGGCACTGGCAATATAAAGCTGTTCTTCATTTTGTTATTCAAAGCGggcgacggcggc > 1:255227/1‑249 (MQ=255) atttcatcggcgccaaagcagcaaaTCAACTTG‑AAGTACCATGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGAGAGTACTA‑CGCCCTGCCGCG‑TTTGTTTTT‑CATCCAAC‑GGGAAAACTCATTTTTTATTGGTAACAGCAG‑TTTACCTGCTTTTATGTCTTCAACCTAACATATAGGCGGCGGTGGCACTGGCAATATAAAGATGTTCTTTCTTGTGTAATTCAACGCGGGCGACGGCGACCTTAtt > 2:244322/23‑251 (MQ=255) aTCGGCGGGCACGTAGTAAATCAACTTG‑AAGTACGCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA‑CGCCCTGCCGCG‑TTTGTTTTG‑CATCCATC‑GGGAATACTCATTTTTAATTGGTAACAGCAG‑TTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTg < 2:31890/251‑1 (MQ=255) taAATCAACTTG‑AAGTACGCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA‑CGCCCTGCCGCG‑TTTGTTTTG‑CATCCATC‑GGGAATACTCATTTTTAATTGGTAACAGCAG‑TTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATT‑CCTGCACGCAACAGGctact < 2:844531/250‑1 (MQ=255) aTCAACGTG‑AAGTACGCTTGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA‑CGCCCTTCCTCG‑TTTGTTTTG‑CATCCATC‑GGGAATACTCATTTTTAATTGGTAACAGCAG‑TTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACtagt < 1:448920/251‑1 (MQ=255) aaCTTG‑AAGTACGCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA‑CGCCCTGCCGCG‑TTTGTTTTG‑CATCCATC‑GGGAATACTCATTTTTAATTGGTAACAGCAG‑TTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGc < 1:471184/251‑1 (MQ=255) aaCTTG‑AAGTACGCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA‑CGCCCTGCCGCG‑TTTGTTTTG‑CATCCATC‑GGGAATACTCATTTTTAATTGGTAACAGCAG‑TTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGc < 1:542592/251‑1 (MQ=255) tG‑AAGTACGCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA‑CTCCCTGCCGCG‑TTTGTTTTG‑CATCCATC‑GGGAATACTCATTTTTAATTGGTAACAGCAG‑TTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAg < 2:474014/249‑1 (MQ=255) gTACGCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA‑CGCCCTGCCGCG‑TTTGTTTTG‑CATCCATC‑GGGAATACTCATTTTTAATTGGTAACAGCAG‑TTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAAc > 1:134124/1‑251 (MQ=255) aCGCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA‑CGCCCTGCCGCG‑TTTGTTTTG‑CATCCATC‑GGGAATACTCATTTTTAATTGGTAACAGCAG‑TTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACg < 2:85577/250‑1 (MQ=255) cGCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA‑CGCCCTGCCGCG‑TTTGTTTTG‑CATCCATC‑GGGAATACTCATTTTTAATTGGTAACAGCAG‑TTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCt > 2:29349/1‑251 (MQ=255) cTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA‑CGCCCTGCCGCG‑TTTGTTTTG‑CATCCATC‑GGGAATACTCATTTTTAATTGGTAACAGCAG‑TTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCg > 1:503128/1‑251 (MQ=255) aaataaaataAGCGGCAAGAGCGAGTAATA‑CGCCCTGCCGCG‑TTTGTTTTG‑CATCCATC‑GGGAATACTCATTTTTAATTGGTAACAGCAG‑TTTACCTGCTTTTATGTCTTCAACCTACCATATAggt < 2:480564/127‑1 (MQ=255) aaataaaataAGCGGCAAGAGCGAGTAATA‑CGCCCTGCCGCG‑TTTGTTTTG‑CATCCATC‑GGGAATACTCATTTTTAATTGGTAACAGCAG‑TTTACCTGCTTTTATGTCTTCAACCTACCATATAggt > 1:480564/1‑127 (MQ=255) taaGCGGCAAGAGCGAGTAATA‑CGCCCTGCCGCG‑TTTGTTTTG‑CATCCATC‑GGGAATACTCATTTTTAATTGGTAACAGCAG‑TTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATc > 1:42869/1‑251 (MQ=255) aGCGGCAAGAGCGAGTAATA‑CGCCCTGCCGCG‑TTTGTTTTG‑CATCCATC‑GGGAATACTCATTTTTAATTGGTAACAGCAG‑TTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCa > 1:412097/1‑250 (MQ=255) | GGTGATTGTTCACCGCCCAGATAAACAGTAGCCAGTTGCCACCAATCAGCACGGCAGAGACTGCCAGCATAAAAATTTTCTGTGGCGTCTGAATCAGCGTTTTTAAATAGGACCACTGGCGGCAAATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTG‑AAGTACGCTGGCGCTAT‑ACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATA‑CGCCCTGCCGCG‑TTTGTTTTG‑CATCCATC‑GGGAATACTCATTTTTAATTGGTAACAGCAG‑TTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCA > NC_000913/4003887‑4004380 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |