BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,265,236	0	A	G	27.5%	242.4 / 19.1	123	I20I (ATT→ATC)	yeiW	putative metal‑chelating domain‑containing protein YeiW
Reads supporting (aligned to +/- strand): ref base A (28/59); new base G (33/0); total (64/59)
Fisher's exact test for biased strand distribution p-value = 6.33e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 124 total aligned reads displayed.

GCGATGGTGATGATTATCGCCACTCTGTTTTGCTTACTGCGGATTAAAGATGTTTAAGGCGCGGTCAGCATTTCCAGATCGATAAGCCAGGTCATGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCAC‑TTGCCCGAAAGG‑CCCGTCGCGAGTACTTTGTCGCGATAT‑TTTTGACATTTTCGACTACAGGAATTTTTCGATGCCAAGAGCGAACGAAATTAAAAAAGGTATGGTAC  >  NC_000913/2264988‑2265486

gCGATGGTGATGATTATCGCCACTCTGTTTTGCTTACTGCGGATTAAAGATGTTTAAGGCGCGGTCAGCATTTCCAGATCGATAAGCCAGGTCATGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAAt                                                                                                                                                                                                                                                                    <  1:525116/251‑1 (MQ=255)

 cGATGGTGATGATTATCGCCACTCTGTTTTGCTTACTGCGGATTAAAGATGTTTAAGGCGCGGTCAGCATTTCCAGATCGATAAGCCAGGTCATGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATa                                                                                                                                                                                                                                                                   <  1:270043/251‑1 (MQ=255)

     ggTGATGATTATCGCCACTCTGTTTTGCTTACTGCGGATTAAAGATGTTTAAGGCGCGGTCAGCATTTCCAGATCGATAAGCCAGGTCATGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGTCATACC‑GGGAATAGGGc                                                                                                                                                                                                                                                               <  1:148407/251‑1 (MQ=255)

      gTGATGATTATCGCCACTCTGTTTTGCTTACTGCGGATTAAAGATGTTTAAGGCGCGGTCAGCATTTCCAGATCGATAAGCCAGGTCATGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCt                                                                                                                                                                                                                                                              <  2:347656/251‑1 (MQ=255)

             ttATCGCCACTCTGTTTTGCTTACTGCGGATTAAAGATGTTTAAGGCGCGGTCAGCATTTCCAGATCGATAAGCCAGGTCATGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGGCC‑TCTGGCATACC‑GGGAATAGGGGTGG‑AAAtt                                                                                                                                                                                                                                                      >  1:516674/1‑251 (MQ=255)

               aTCGCCACTCTGTTTTGCTTACTGCGGATTAAAGATGTTTAAGGCGCGGTCAGCATTTCCAGATCGATAAGCCAGGTCATGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGAAGGGCGTATTGGCGGGGTTGTCACTCTG‑CATACC‑CGGGATAGGGCGGG‑AAATT‑gg                                                                                                                                                                                                                                                   >  2:693265/1‑250 (MQ=255)

                   ccACTCTGTTTTGCTTACTGCGGATTAAAGATGTTTAAGGCGCGGTCAGCATTTCCAGATCGATAAGCCAGGTCATGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTTCCA‑TCTGGCCTACCCGGGAA‑AGGGGTGG‑GAAAT‑G‑AAggg                                                                                                                                                                                                                                              >  2:183520/1‑251 (MQ=255)

                    cACTCTGTTTTGCTTACTGCGGATTAAAGATGTTTAAGGCGCGGTCAGCATTTCCAGATCGATAAGCCAGGTCATGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCGTGCCA‑TCTGGCATACC‑GGGGATAGGGGTGG‑AAATT‑G‑TAgggg                                                                                                                                                                                                                                             >  1:260304/1‑251 (MQ=255)

                      ctctGTTTTGCTTACTGCGGATTAAAGATGTTTAAGGCGCGGTCAGCATTTCCAGATCGATAAGCCAGGTCATGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGGTTGCCC‑TCTGGCATACC‑GGGGATaagggggggaattggagggggc                                                                                                                                                                                                                                              >  2:148550/1‑230 (MQ=255)

                       tctGTTTTGCTTACTGCGGATTAAAGATGTTTAAGGCGCGGTCAGCATTTCCAGATCGATAAGCCAGGTCATGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGc                                                                                                                                                                                                                                            >  1:112603/1‑249 (MQ=255)

                         tGTTTTGCTTACTGCGGATTAAAGATGTTTAAGGCGCGGTCAGCATTTCCAGATCGATAAGCCAGGTCATGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGGTTGCCA‑TCTCGCCTTCC‑GGGAATAAGGCTGG‑AAATT‑G‑AAgggggaggg                                                                                                                                                                                                                                        >  2:398403/1‑246 (MQ=255)

                          gTTTTGCTTACTGCGGATTAAAGATGTTTAAGGCGCGGTCAGCATTTCCAGATCGATAAGCCAGGTCATGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGACCATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTTTCA‑GCTGGCAAACC‑CGGTATATGGCTGG‑GAAAT‑G‑CAgggggaggtg                                                                                                                                                                                                                                       >  2:257531/1‑245 (MQ=255)

                               gCTTACTGCGGATTAAAGATGTTTAAGGCGCGGTCAGCATTTCCAGATCGATAAGCCAGGTCATGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAacac                                                                                                                                                                                                                                  <  2:425631/251‑1 (MQ=255)

                                      gCGGATTAAAGATGTTTAAGGCGCGGTCAGCATTTCCAGATCGATAAGCCAGGTCATGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATGCC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCg                                                                                                                                                                                                                           <  2:183696/250‑1 (MQ=255)

                                        ggATTAAAGATGTTTAAGGCGCGGTCAGCATTTCCAGATCGATAAGCCAGGTCATGGCCTGCTGATGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGGAAAGGGGGGG‑AAATTTG‑AAGGGG‑AGTGCAACAC‑ACCCCGCac                                                                                                                                                                                                                        >  1:200973/1‑250 (MQ=255)

                                         gATTAAAGATGTTGAAGACGCGGTCCGAATTTCCAGATCGATAAGACAGGTCATGGCCGGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAACTGTAACAGCGGTGCTGTTCATCAAGCTGAATGCAGGGCGTATCGGCGGGATTTCTC‑TCCTGCATACC‑GCGTATAGGGATGG‑AAGTT‑G‑AAgagggaatggataacgacccgcaac                                                                                                                                                                                                                        >  2:450031/1‑227 (MQ=255)

                                           ttAAAGATGTTTACGGCGCGGTCAGCATTTCCAGATCGATCAGCCAGGTCATGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTTCCA‑TCCCGCACACC‑GGGGATAGGGGTGG‑ATATT‑G‑ACAGGGCAGTGCCACAC‑GCCCCCCAAccc                                                                                                                                                                                                                     >  2:245116/1‑251 (MQ=255)

                                            tAAAGATGTTTAAGGCGCGGTCAGCATTTCCAGATCGATAAGCCAGGTCATGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTTGCA‑TCTGGCATACC‑GGGAATAGGGGTGG‑GAATT‑G‑GAGGGGCAGTGCAAAAC‑GCCCCGCaaaccc                                                                                                                                                                                                                    >  1:589655/1‑247 (MQ=255)

                                               aGATGTTTAAGGCGCGGTCAGCATTTCCAGATCGATAAGCCAGGTCATGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGGTTTCCA‑TCTGGCATACC‑GGGGATAGGGGGGG‑GAATT‑G‑TAAGGGCAGGGCAACAC‑GCCCCGCCACCCgatc                                                                                                                                                                                                                 >  2:789459/1‑248 (MQ=255)

                                               aGATGTTTAAGGCGCGGTCAGCATTTCCAGATCGATAAGCCAGGTCATGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTTGCA‑TCTGGCCTACC‑GGGGGAAGGGCTGG‑GAATT‑G‑GAGGGGGAGTGCAACCCCGCCCCCCAAccgggc                                                                                                                                                                                                                  >  2:152754/1‑247 (MQ=255)

                                               aGATGTTTAAGGCGCGGTCAGCATTTCCAGATCGATAAGCCAGGTCATGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAAcc                                                                                                                                                                                                                      <  1:789459/246‑1 (MQ=255)

                                               aGATGTTTAAGGCGCGGTCAGCATTTCCAGATCGATAAGCCAGGTCATGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCCCAACCCGGAc                                                                                                                                                                                                                 >  1:488156/1‑251 (MQ=255)

                                               aGATGTTTAAGGCGCGGTCAGCATTTCCAGATCGATAAGCCAGGTCATGGCCGGCTGACGCGAGTTACCGCACATTCCAGCGCTGGCTTGTAAGCCTGCACAGACGTGCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGAc                                                                                                                                                                                                                 <  2:209244/251‑1 (MQ=255)

                                                 aTGTTTAAGGCGCGGTCAGCATTTCCAGATCGATAAGCCAGGTCATGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACgg                                                                                                                                                                                                               <  1:693344/251‑1 (MQ=255)

                                                     ttAAGGCGCGGTCAGCATTTCCAGATCGATAAGCCAGGTCATGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCAA‑TCTAGCATACC‑GGGAATAGGGCTGG‑AGATT‑G‑AAGGGGCCGTGCAAAAC‑GCCCCGCAACCCGGACggaact                                                                                                                                                                                                           >  1:168261/1‑247 (MQ=255)

                                                        aGGCGCGGTCAGCATTTCCAGATCGATAAGCCAGGTCATGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGGTTGCCA‑TCTGGCATACC‑GGGGATAGGGCTGG‑GAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACCGCC‑TTcc                                                                                                                                                                                                        >  1:34080/1‑250 (MQ=255)

                                                           cgcgGTCAGCATTTCCAGACCGATAAGCCAGGTCATGGCCTGCTGACGCGAGTTACCGCCCATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TAc                                                                                                                                                                                                   <  2:455691/251‑1 (MQ=255)

                                                               gTCAGCATTTCCAGATCGATAAGCCAGGTCATGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGGATAGGGGTGG‑AAATT‑G‑AAGGGGCAGAGCAACAC‑CCCCCGCACCCCGGACGGCA‑TTCCC‑TACgttg                                                                                                                                                                                               >  1:308508/1‑251 (MQ=255)

                                                                          cAGATCGATAAGCCAGGTCATGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAgg                                                                                                                                                                                    <  1:148550/251‑1 (MQ=255)

                                                                            gATCGATAAGCCAGGTCATGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGGATAGGGCTGG‑GAAAT‑G‑AAGGGGCAGTGCCACAC‑GCCCCGCAAACCGGGCGGGG‑TTCCA‑TACGTTGTTCTCTTAAgggt                                                                                                                                                                                  >  1:573651/1‑249 (MQ=255)

                                                                             aTCGATAAGCCAGGTCATGGCCTGCTGACGCGAGGTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAg                                                                                                                                                                                     <  1:507312/247‑1 (MQ=255)

                                                                               cGATAAGCCAGGTCATGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGGATAGGGCTGG‑AAATT‑G‑AAGGGGCAAGGCAAAAC‑GACCCGCAACCCGGACGGCA‑TTCCA‑TCCGTTGTTCTCTTAAGGTTAac                                                                                                                                                                               >  1:297150/1‑251 (MQ=255)

                                                                               cGATAAGCCAGGTCATGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑GAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAac                                                                                                                                                                               >  1:311558/1‑251 (MQ=255)

                                                                                       cAGGTCATGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTTCCC‑TCTGGCATACC‑GGGAATAGGGCTGG‑GAATT‑G‑CAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCT‑TTCCC‑TACGTTTTTCTCTTAAGGTTTACCA‑CCGGAt                                                                                                                                                                      >  1:336672/1‑251 (MQ=255)

                                                                                             aTGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGGTTGCCC‑ACTGGCATACC‑GGGGGTAGGGCTGG‑GAATT‑G‑CAGGGGGGGTGGAAAAC‑GCACCGCAAACCGGTAGGCACTTCC‑‑TACGTTGTTctctgtaggtgagccaccgcgattgtgg                                                                                                                                                                 >  2:460030/1‑227 (MQ=255)

                                                                                             aTGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATtgatga                                                                                                                                                                <  1:126715/251‑1 (MQ=255)

                                                                                               ggctggctgGCGCTAGTGACCGCACATTCTGGCGTTGGCTTGTAAGCCTGCACAGCCTGTGATTCGCAGTGGCGAGGTTAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCTGGCTTGCGA‑TCTGTCATACT‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCTCCGCAACCCGTACGGCA‑GTCCA‑TACGTTGGTCTCCTAAGGTTAACCG‑CCGGTTtgatgtgc                                                                                                                                                              <  2:667776/246‑4 (MQ=255)

                                                                                               ggCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAgc                                                                                                                                                              <  1:692167/251‑1 (MQ=255)

                                                                                                    gctgACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGca                                                                                                                                                         <  1:709694/251‑1 (MQ=255)

                                                                                                              gTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTTCCA‑TCTGGCATACC‑GGGGATAGGGCTGG‑GAATT‑G‑AAGGGGCAGTGCAACAC‑CCCCCGCAACCCCGGCGGCA‑TTTCA‑TACGTTTTTCTCTTTAGGTTTACCA‑CCGGATTGTTGAGCGCGCACAGTaccacc                                                                                                                                               >  1:11515/1‑251 (MQ=255)

                                                                                                                tACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGGATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAAAAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAAGTTAACCA‑CCGGATTGATGAGCGCGCACAGTaacaccct                                                                                                                                             >  1:572053/1‑249 (MQ=255)

                                                                                                                   cGCACATTTCAGCGCGGGCTTGTAAGCCTGCCGAGACTTTCGGTCGGAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATGCC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑GTCCA‑TACGTTGTTCTCTTAAGTTTAACCA‑CCGGATTGATGAGCGCTCTCAGTACCACCttttt                                                                                                                                          <  2:279011/251‑1 (MQ=255)

                                                                                                                    gcgcaTTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCG‑CCGGTTTGATGAGCGCGCACATTACCACCtttttt                                                                                                                                         <  2:653646/248‑1 (MQ=255)

                                                                                                                       caTTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCa                                                                                                                                      <  2:797826/251‑1 (MQ=255)

                                                                                                                       caTTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCa                                                                                                                                      <  1:245349/251‑1 (MQ=255)

                                                                                                                             aGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TAAGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGc                                                                                                                                 >  1:171182/1‑250 (MQ=255)

                                                                                                                             aGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGCTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGGGTTCCC‑TCTGGCAAACC‑GGGGATAGGGGTGGGAAATT‑‑‑AAGGGGGAGTGCAACAC‑CCCCCGCCACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAAGTGAACCAACCGG‑CTGCTGAGCGCGCCCCGTACCCCCCTTTTTGCAcccca                                                                                                                                 >  2:268051/1‑247 (MQ=255)

                                                                                                                                 cTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGGATAGGGCTGG‑GAATT‑G‑AAAGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCC‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTTCACCAGCcaaa                                                                                                                             >  1:553632/1‑250 (MQ=255)

                                                                                                                                     cTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCCTACC‑GGGGATAGGGCTGG‑GAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAAACCGGACGGCA‑ATACA‑TACGTTGTTCTCTTAAGGATAACCC‑CCGGATTTATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCaaaaatgtg                                                                                                                        >  1:169198/1‑246 (MQ=255)

                                                                                                                                       tGTAAGCCTGCACAGACGTTCGGTCGCAGTGGCGAGGTGAAAAGTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCg                                                                                                                        <  2:153085/249‑1 (MQ=255)

                                                                                                                                        gTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAAt                                                                                                                     <  1:398403/251‑1 (MQ=255)

                                                                                                                                          aaGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATAc                                                                                                                   <  1:335819/251‑1 (MQ=255)

                                                                                                                                                gCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCAC‑tt                                                                                                             <  2:112603/250‑1 (MQ=255)

                                                                                                                                                  acaGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCC‑TCTGGCATAAC‑GGGGATAGGGATGG‑GAATT‑GGAAGGGG‑AGTTCCACAC‑GCCCCCCAACCCGGCCGGGA‑GTTCA‑TACGTTGTTCTCTTAAGGATAACCA‑CCGGATTTATTAGCGCGCACAGTACCCCCCTTTTTGCACCAgtgatcggaatagcaaacgtctgg                                                                                                           >  1:120307/1‑228 (MQ=255)

                                                                                                                                                  acaGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAg                                                                                                                                  <  2:120307/228‑1 (MQ=255)

                                                                                                                                                   caGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCAC‑TTGccc                                                                                                         <  2:813115/251‑1 (MQ=255)

                                                                                                                                                   caGACTTTCGGTCGCAGAGGCGAGGAGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCCAACC‑GGGGATAGGGCTGG‑GAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGGGCGCACAGTACCACCTTTTTTGCTCCAGCAAAAATGCGAATACCAC‑TTGccc                                                                                                         >  1:253189/1‑251 (MQ=255)

                                                                                                                                                        tttCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGGATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATAACAC‑TTGCCCGaa                                                                                                      >  1:135429/1‑249 (MQ=255)

                                                                                                                                                           cGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCAC‑TTGCCCGAAAGG‑c                                                                                                 <  1:61224/250‑1 (MQ=255)

                                                                                                                                                            ggTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGCATAGGGCTGG‑AAATT‑G‑GAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAAACA‑CCGGATTGGTGAGCGCGCACCGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCAC‑CTGCCCCAACGG‑ccc                                                                                               >  1:663567/1‑251 (MQ=255)

                                                                                                                                                             gTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GTGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCAC‑TTGCCCGAAAGG‑CCCg                                                                                              <  2:332137/251‑1 (MQ=255)

                                                                                                                                                             gTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGGATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGAGCGAATACCAC‑TTGCCCGAAAGG‑CCCg                                                                                              >  1:579550/1‑251 (MQ=255)

                                                                                                                                                                gCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCAC‑TTGCCCGAAAGG‑CCCGTcg                                                                                           <  1:93130/251‑1 (MQ=255)

                                                                                                                                                                    tGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTTCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑CAATT‑G‑AAGGGGGCGTGCCACAA‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGATAACCC‑CCGGATTGATGAGGGCGCACAGTACAACCTTTTTTTCACCAGCAAAAATGAGAATACCAC‑TTACCCGAAAGG‑ccaggcgcg                                                                                         >  1:145649/1‑242 (MQ=255)

                                                                                                                                                                        gAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCAC‑TTGCCCGAAAGG‑CCCGTCGCGAGTACt                                                                                   <  1:309987/251‑1 (MQ=255)

                                                                                                                                                                           gTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCCTACC‑GGGGATAGGGCTGG‑AAATT‑G‑AAGGGGGAGTGCAACAC‑GCCCCGCAACCCGGACGGCC‑ATCCA‑TACGTTGTTCTCTTAAGGGTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGAAAAAATGCGAATACAAC‑CTGCCCGCAAGG‑CCCGTCGCGAGTAAtttt                                                                                >  1:41479/1‑250 (MQ=255)

                                                                                                                                                                             gAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGGATAGGGCTGG‑GAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGGTAACCA‑CCGGGTTGATGAGCGCGCACAGTACCACCTTTTTTTCACCAGCAAAAGAGCGAATAACAC‑TTGCCCGGAAGG‑CCCGTCGCGAGTACtttttt                                                                              >  2:483382/1‑248 (MQ=255)

                                                                                                                                                                                aaTTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGTCGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCTC‑TTGCCCGAAAGG‑CCGGTCGCGAGTACTTTGTcgcg                                                                           <  2:190781/251‑1 (MQ=255)

                                                                                                                                                                                      aCAGCGTTGCTGTTCATCAAGCTGAATGCCGGGCGTATTGGCGGTCTTGCCC‑TCTGGCCTACC‑GGGGAAAAGGCTGG‑AAATT‑G‑AAAGGGCAGAGCAACAA‑CCCCCGCCACCCGGCCGGAA‑TTCCAATCCGT‑GTTTTCTTAAGGTTAAACA‑CCCGGTTGATGAGCGCCCACAGGACCCCCCTTTTTACACCCACAAAAGAGCCAACACAAC‑ATTCCCCAAAGG‑CCCGGCCCGAGTACtttttgccgcaactt                                                                     >  2:601327/1‑239 (MQ=255)

                                                                                                                                                                                      aCAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCAC‑TTGCCCGAAAGG‑CCCGTCGCGAGTACTTTGTCGCGATAt‑t                                                                     <  1:473454/250‑1 (MQ=255)

                                                                                                                                                                                        aGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTTGCATCCC‑GGGAATAGGGCCGG‑AAATT‑G‑AAGGGGCAGGGGAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCC‑TAAGATGTTCTCTTAAGGATAACCA‑ACGGATTTCTGGTCGCGCACAGTACCACCCTTTTTTCACCCGCAAAAAAGGGAATACCAC‑TTGCCCGAAAAG‑CCCGTCGCGAGTTCATTGTTGCGCTAt‑tttt                                                                  >  2:619461/1‑251 (MQ=255)

                                                                                                                                                                                        aGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGCGTTGCCA‑TCTGGTCTACC‑GGGGGAAGGGCTGG‑GAATT‑G‑AAGGGGCAGGGGAACAC‑GCCCCGCAACCCCGTAGGCA‑TTTCA‑TACGTTGTTCTCTTAAGGGTAACCA‑CCGGATAGAGGAGCGCGCACAGTACCACCTTTTTTTCACCAGCAAAAATGAGGATAACCC‑TTGACCGAAAGG‑CCCGGCTCGAGTACTTTGTCGCGATAg‑tttt                                                                  >  2:405442/1‑251 (MQ=255)

                                                                                                                                                                                           gTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCCGGCACACC‑GGGAATAGGGGTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGGCGGCA‑ATCCC‑TACGATGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGAGCGAATACCAC‑TTGCCCGAAAGG‑CACGTCGCGAGTACTTTGTCGCGATAT‑TTTTGAc                                                               >  1:35922/1‑251 (MQ=255)

                                                                                                                                                                                           gTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCCTGCCA‑TCTTGCATACC‑GGGAATAGGGGTGG‑AAATT‑G‑AAGGGGCCGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGGTTGATGAGCGCGCACAGTACCACCTTTTTTTCACCAGCAAAAGTGCGAATACCAC‑TTGCCCGAAAGG‑CCCGTCGCGAGTACCTTGTCGCGATAT‑TTTTGAc                                                               >  1:259855/1‑251 (MQ=255)

                                                                                                                                                                                                 gTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAT‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCAC‑TTGCCCGAAAGG‑CCCGTCGCGAGTACTTTGTCGCGATAT‑TTTTGACAtttt                                                          <  1:600635/250‑1 (MQ=255)

                                                                                                                                                                                                   tcatcaAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCAC‑TTGCCCGAAAGG‑CCCGTCGCGAGTACTTTGTCGCGATAT‑TTTTGACATTTTCg                                                        <  2:488156/250‑1 (MQ=255)

                                                                                                                                                                                                    catcaAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCCTACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGGGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAATGCGAATACCAC‑TTGCCCGAAAGG‑CCCGTCGCGAGTACTTTGTCGCGATAT‑TTTTGACATTTTCGAc                                                      >  1:540718/1‑251 (MQ=255)

                                                                                                                                                                                                        aaGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCAC‑TTGCCCGAAAGG‑CCCGTCGCGAGTACTTTGTCGCGATAT‑TTTTGACATTTTCGACTACa                                                  <  1:183520/251‑1 (MQ=255)

                                                                                                                                                                                                             gAATGCAGGGCGTATTGGCGGGCTTGTCA‑TCCGGAATACC‑GGGACTAGGGCTGG‑AAATT‑G‑AAGGGGTAGGGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCAA‑TTAGTTGTTCTCTTAAGGTTAAACA‑CCGGATTGATGAACGCACACAGTAACACCTTTTTTGTCCCAGCAAAAGTGAGAATAC‑ACATTGCCCGAAAGG‑CCCGTCGCGAGTACTTTGTCGCGATCT‑TTTTGACAtttcatactaccggaat                                             >  1:497978/1‑237 (MQ=255)

                                                                                                                                                                                                             gAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCAC‑TTGCCCGAAAGG‑CCCGTCGCGAGTACTTTGTCGCGATAT‑TTTTGACATTTTCGACTACAGGa                                               <  1:147598/249‑1 (MQ=255)

                                                                                                                                                                                                              aaTGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGGATAGGGCTGG‑AAATT‑G‑AAGGGGGAGTGCAACAC‑GCCCCGCAACCCGGGCGGGA‑TTCCA‑TACGTTGTTCTCTTTAGGTTAACCC‑CCGGGTTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGGGCGGATAACAC‑TTGCCCCAAAGGGCCCG‑CGCGCGTACTTTGTCGCGATAT‑TTTTGACATTTTTGACTACAGGAAtt                                            >  2:496507/1‑251 (MQ=255)

                                                                                                                                                                                                                 gCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGTAGTGCAACAC‑GCCTCGCAACCCGGACGGCA‑TGCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCTT‑TTTCCCTAAAGG‑CCCGTCGCGAGTACTTTGTCGCGCTAT‑TTTTTACATTTTCGACTACAGGAAttttt                                         <  2:460971/251‑1 (MQ=255)

                                                                                                                                                                                                                 gCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCAC‑TTGCCCGAAAGG‑CCCGTCGCGAGTACTTTGTCGCGATAT‑TTTTGACATTTTCGTCTACAGGAAtttt                                          <  2:553632/250‑1 (MQ=255)

                                                                                                                                                                                                                    gggCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCAC‑TTGCCCGAAAGG‑CCCGTCGCGAGTACTTTGTCGCGATAT‑TTTTGACATTTTCGACTACAGGAATTTTTCgg                                      >  1:66625/1‑250 (MQ=255)

                                                                                                                                                                                                                     ggCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑TGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCAC‑TTGCCCGAAAGG‑CCCGTCGCGAGTACTTTGTCGCGATAT‑TTTTGACATTTTCGACTACAGGAATTTTTCGAt                                     <  1:328119/251‑1 (MQ=255)

                                                                                                                                                                                                                     ggCGTAGTGGCGGGCTTGTCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAGCAC‑GCCCCGGAACCCGGACGGCA‑TTCCA‑TACGTGGTTCTCTTAAGGTTAACCA‑CCGGAGTGATGAGCGCGCACAGTACCTCCTTGTTTGCGCGAGCAAAAGTGCGAATACCGC‑TTGCCCTAATGG‑CCCGGCGCGAGTACTTCGTCGTGATATGTTTTG‑CAGTTGCTACTTCAGGAATTTTTCGAt                                     <  2:612018/251‑1 (MQ=255)

                                                                                                                                                                                                                        gTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCAC‑TTGCCCTAAAGG‑GCCGTCGCGAGTACTGTGGCGCGATAT‑TTTTGGCATTTTCGACTTCAGGAATTTTTCGATGcc                                  <  2:740000/251‑1 (MQ=255)

                                                                                                                                                                                                                           ttGGCGGGCTTGCCA‑TCTGGAATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCAC‑TTGCCCGAAAGG‑CCCGTCGCGAGTACTTTGTCGCGATAT‑TTTTGACATTTTCGACTACAGGAATTTTTCGATGCCa                                 >  2:613731/1‑249 (MQ=255)

                                                                                                                                                                                                                             ggCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCAC‑TTGCCCGAAAGG‑CCCGTCGCGAGTACTTTGTCGCGATAT‑TTTTGACATTTTCGACTACAGGAATTTTTCGATGCCAag                               >  2:586569/1‑249 (MQ=255)

                                                                                                                                                                                                                                  gCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCAGCAACCCGGACGGCA‑TTCCA‑TAAGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCAC‑TTGCCCGAAAGG‑CCCGTCGCGAGTACTTTGTCGCGATAT‑GTGTGACATTTTCGACTACAGGAATTTTTCGATGCCAAGAGcgaac                        <  2:612218/251‑1 (MQ=255)

                                                                                                                                                                                                                                        cA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCAC‑TTGCCCGAAAGG‑CCCGTCGCGAGTACTTTGTCGCGATAT‑TTTTGACATTTTCGACTACAGGAATTTTTCGATGCCAAGAGCGAACGAAAtt                  <  1:191453/251‑1 (MQ=255)

                                                                                                                                                                                                                                        cA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCCTTTTTGCACCAGCAAAAGTGCGAATACCAC‑TTGCCCGAAAGG‑CCCGTCGCGAGTACTTTGTCGCGATAT‑TTTTGACATTTTCGACTACAGGAATTTTTCGATGCCAAGAGCGAACGAAAtt                  >  1:426354/1‑251 (MQ=255)

                                                                                                                                                                                                                                             tGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCAC‑TTGCCCGAAAGG‑CCCGTCGCGAGTACTTTGTCGCGATAT‑TTTTGACATTTTCGACTACAGGAATTTTTCGATGCCAAGAGCGAACGAAATTaaa               >  1:38306/1‑250 (MQ=255)

                                                                                                                                                                                                                                               gCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCAC‑TTGCCCGAAAGG‑CCCGTCGCGAGTACTTTGTCGCGATAT‑TTTTGACATTTTCGACTACAGGAATTTTTCGATGCCAAGAGCGAACGAAATTaaaaaa            <  2:826626/251‑1 (MQ=255)

                                                                                                                                                                                                                                                cATACC‑GGGAATAGGGCTGG‑AAATT‑G‑ACGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCAC‑tt                                                                                                             >  1:161359/1‑155 (MQ=255)

                                                                                                                                                                                                                                                cATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCAC‑tt                                                                                                             <  2:161359/155‑1 (MQ=255)

                                                                                                                                                                                                                                                   aCC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCAC‑TTGCCCGAAAGG‑CCCGTCGCGAGTACTTTGTCGCGATAT‑TTTTGACATTTTCGACTACAGGAATTTTTCGATGCCAAGAGCGAACGAAATTAAAAAAGGt         >  1:509618/1‑250 (MQ=255)

                                                                                                                                                                                                                                                    cc‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCAC‑TTGCCCGAAAGG‑CCCGTCGCGAGTACTTTGTCGCGATAT‑TTTTGACATTTTCGACTACAGGAATTTTTCGATGCCAAGAGCGAACGAAATTAAAAAAGGTAt       <  2:486341/251‑1 (MQ=255)

                                                                                                                                                                                                                                                     c‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCAC‑TTGCCCGAAAGG‑CCCGTCGCGAGTACTTTGTCGCGATAT‑TTTTGACATTTTCGACTACAGGAATTTTTCGATGCCAAGAGCGAACGAAATTAAAAAAGGTATg      <  1:492217/251‑1 (MQ=255)

                                                                                                                                                                                                                                                          aaTAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCAC‑TTGCCCGAAAGG‑CCCGTCGCGAGTACTTTGTCGCGATAT‑TTTTGACATTTTCGACTACAGGAATTTTTCGATGCCAAGAGCGAACGAAATTAAAAAAGGTATGGTAc  <  2:572053/251‑1 (MQ=255)

GCGATGGTGATGATTATCGCCACTCTGTTTTGCTTACTGCGGATTAAAGATGTTTAAGGCGCGGTCAGCATTTCCAGATCGATAAGCCAGGTCATGGCCTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCA‑TCTGGCATACC‑GGGAATAGGGCTGG‑AAATT‑G‑AAGGGGCAGTGCAACAC‑GCCCCGCAACCCGGACGGCA‑TTCCA‑TACGTTGTTCTCTTAAGGTTAACCA‑CCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGAATACCAC‑TTGCCCGAAAGG‑CCCGTCGCGAGTACTTTGTCGCGATAT‑TTTTGACATTTTCGACTACAGGAATTTTTCGATGCCAAGAGCGAACGAAATTAAAAAAGGTATGGTAC  >  NC_000913/2264988‑2265486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`