| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,248,424 | 0 | C | A | 26.7% | 128.1 / 18.5 | 60 | V37F (GTT→TTT) | lysP | lysine:H(+) symporter |
| Reads supporting (aligned to +/- strand): ref base C (39/5); new base A (0/16); total (39/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.36e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAAC‑GAACCGGAAACC‑GGCATATATGCAGCCAGTTCACCGAGACTGGTCATCA‑GGAAGTAAACCATCAGGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCG > NC_000913/2248186‑2248672 | gCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAAC‑GAACCGGAAACC‑GGCATATATGCAGCCAGTTCACCGAGACTGGTCATCA‑GGAAGTAAACCATCAGGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTg < 1:554855/250‑1 (MQ=255)gCAACCAGGCCACCGCCGCTAGTCAGCGCCCAGTTGTAGTAGTATGTCGTGCACAGCGCGAAGCCAATGCCTTCTTCAACATAGTTCTGACCGTAAGCGGCAAAC‑GACCCTGAAACC‑GGCATATATGCAGCCAGTTCACCGACACGGGTCATCA‑GGAATTAAACCATCAGGCTAAACTGCATAAACAA‑GAGCAATGCCCCGCCCGGTCCCGCCTGAGAAATCGTTTCGCCAGAGGccacaataatacccg < 2:716919/250‑10 (MQ=255) aaCGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCCAAGCCTTCTTCAACATAGGTCTGACCGTAAGTGGCAAAC‑GAACCGGAAACC‑GGCATATATGCAGCCAGTTCACCGAGACTGGTCATCA‑GGAAGTAAACCATCAGGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGACCTGCCTGCGAAATCGTTGCGCCAAAGGCAACAAAAAGACCTG‑TACCGATGGAAc > 2:552334/1‑251 (MQ=255) aaCGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAAC‑GAACCGGAAACC‑GGCATATATGCAGCCAGTTCACCGAGACTGGTCATCA‑GGAAGTAAACCATCAGGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑AACCGATGGaa > 2:833856/1‑250 (MQ=255) gATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAAC‑GAACCGGAAACC‑GGCATATATGCAGCCAGTTCACCGAGACTGGTCATCA‑GGAAGTAAACCATCAGGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACA‑GCCa > 2:525895/1‑250 (MQ=255) tagtTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAAC‑GAACCGGAAACC‑GGCATATATGCAGCCAGTTCACCGAGACTGGTCATCA‑GGAAGTAAACCATCAGGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACgcgc > 1:107237/1‑251 (MQ=255) ccagcccagcGCGAAACCAAACCCTTCTTAAACATAGTCATGACCGTAAGGCGAAAAC‑AAACCGGAAACC‑GTCATATAGGCACCCAGTGCGCCGACACGGGTAATCA‑AGAAGAAAACCCACGGGCCAACCAGCAAAAGGGA‑GAGAAATCCCCCGCCCGGACCTGCCTGAAAAAGCGTGGCCCGAGAGGAAAAAAAAAGCCCGG‑CCCCGACGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTa < 2:22371/251‑1 (MQ=255) gcccagcGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAAC‑GAACCGGAAACC‑GGCATATATGCAGCCAGTTCACCGAGACTGGTCATCA‑GGAAGTAAACCATCAGGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTTAGGTGACGCGCCTTTAAtt > 1:679642/1‑251 (MQ=255) agcGCGAAGCCAAAGCCTTCTTCTACATAGTTATGACCGTAAGTGGCAACC‑GAACCGGAAACC‑GGCATATATGCAGCCAGTTCACCGAGACTGGTCATCA‑GGAAGTAAACCATCAAGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCAGAACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAACAGACCTG‑TACCGATGCAACC‑GCCAATGGCAATCATCGTCAGGCGACGCGCCTTTAATTCACg > 2:103493/1‑251 (MQ=255) gcgcGAACACAAAGCCTTCTAAAACATGGTTCGGACCGAAAGGGGAAAAC‑GAACCGAAACCC‑GGCATATATGCAGCCAGTCCGCCGAGACTGGTCATCA‑GAAAGAAAACCATCGGGCAAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGACCGGCCGAAGAAATCGTTGCGCAAGGGGCAAAAAAAAGCCCTT‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACgg < 1:283513/251‑1 (MQ=255) gCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAAC‑GAACCGGAAACC‑GGCATATATGCAGCCAGTTCACCGAGACTGGTCATCA‑GGAAGTAAACCATCAGGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGCCCTGCCTGAGAAAGCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TCCCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGc < 1:553318/251‑1 (MQ=255) aaaGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAAC‑GAACCGGAAACC‑GGCATATATGCAGCCAGTTCACCGAGACTGGTCATCA‑GGAAGTAAACCATCAGGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCg > 2:583269/1‑251 (MQ=255) aGCCTTTTTCAAAATAGTCAGGACCGAAAG‑GGCAAACCGAACCGTAACCC‑GGAAATTATGCACCCAGTTCACCGAGCGTGGTCATCA‑GGAATAAAACCATGAGCCAAACCACCATATGGTA‑GAGTAATGCCCCGCCCGGTCTGGCCTGAAAAATGGTTGCGCCAGAGGCAAAAAAAAGACCTG‑TTCCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGgc < 1:145680/251‑1 (MQ=255) aGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAAC‑GAACCGGAAACC‑GGCATATATGCAGCCAGTTCACCGAGACTGGTCATCA‑GGAAGTAAACCATCAGGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAatc > 1:651895/1‑209 (MQ=255) aGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAAC‑GAACCGGAAACC‑GGCATATATGCAGCCAGTTCACCGAGACTGGTCATCA‑GGAAGTAAACCATCAGGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGgc > 2:710857/1‑251 (MQ=255) aGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAAC‑GAACCGGAAACC‑GGCATATATGCAGCCAGTTCACCGAGACTGGTCATCA‑GGAAGTAAACCATCAGGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGgc > 1:574240/1‑251 (MQ=255) ccctttttaaaaatatttCTGACCGAAAGGGACAAA‑‑GAACCGGGCACCCGGCATATATGCAGCTGTTTCACGGAGCCGGGCCATAA‑GAAAGTAACCCACCGGGCCAACCACCATATACAC‑GAGAAGTGCCCCGCCCGGCCTTGCCGGAAAATTCGTTGCGCAAGAGGAAAAAAAAAGCCTTG‑TCCCGAGGGATCC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGgcg < 1:651921/235‑1 (MQ=255) aaCATAGTTCTGACCGTAAGTGGCAAAC‑GAACCGGAAACC‑GGCATATATGCAGCCAGTTCACCGAGACTGGTCATCA‑GGAAGTAAACCATCAGGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAgg > 1:44785/1‑208 (MQ=255) tttggcccaaaaggggaaaaC‑GAACCGAAACCC‑GGCATATATGCCGCCAGTTCCCCGAGCCGGGTCAAAG‑GGAAGAAACCCACCAGGCAAATCAGCATATACGG‑GAGCAATCCCCCGCCCGGACCGGCCTGAAAAATCGTTGCGCCAGAGGCAAAAAAAAGCCCTG‑TCCCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAg < 1:740272/233‑1 (MQ=255) acagTAAGAGGCAAAC‑GAACCGGAAACC‑GGCATATATTCAGCCAGATCACCGAGACTGGTCATCC‑GGAAGTAAACCATCAGGCCACTCAGCATATACGA‑GAGCAATGCCCCTCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCTTTTACTTACCGCCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTctg > 2:673789/4‑249 (MQ=255) aCCGTAAGTGGCAAAC‑GAACCGGAAACC‑GGCATATATGCAGCCAGTTCACCGAGACTGGTCATCA‑GGAAGTAAACCATCAGGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCgg > 2:751950/1‑251 (MQ=255) aGTGGCAAAC‑GAACCGGAAACC‑GGCATATATGCAGCCAGTTCACCGAGACTGGTCATCA‑GGAAGTAAACCATCAGGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAAcc > 1:821025/1‑250 (MQ=255) ccGGAAACC‑GGCATATATGCAGCCAGTTCACCGAGACTGGTCATCA‑GGAAGTAAACCATCAGGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAACGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCACGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACACAAATGCtt > 2:71590/1‑250 (MQ=255) ggAAACC‑GGCATATATGCAGCCAGTTCACCGAGACTGGTCATCA‑GGAAGTAAACCATCAGGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATc > 1:141650/1‑251 (MQ=255) ggAAACC‑GGCATATAGGCAGCCAGTCCACCAAGAC‑GGGCAACAAGAAAGAAAACCACCAGCCCAATCAGCATATCGGA‑GAGCAAGCCCCCGCCCGGCCCTGCCTGAAAAATCGTGCCGCAAGAGGAAAAAAAAAGCCCTG‑TCCCGAGGAAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATc < 1:383288/251‑1 (MQ=255) gcaaCC‑GGCATATATGCACCCAGTTCCCCGAGACTGGTCATCA‑GGAAGTAAACCATCAGGCCAATCAGCATATACGC‑GAGCAATGCCCCGCCCGGACCTCCCTGAGCAATCGTTGCGCCAGAGGCGACAAACAGACCTG‑TACCGCTGGAACC‑GCCAATGGCAGTCATCGTCAGGTGCCGCGCCTTTAATTACCCGCGTAAGCCCGGCGCTTCTGTGGTTTTAATTTCGGAATCCATACAAACATGATATcc > 2:399329/3‑251 (MQ=255) ggCATATATGCAGCCAGTTCACCGAGACTGGTCATCA‑GGAAGTAAACCATCAGGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCtt > 1:469476/1‑250 (MQ=255) cATATATGCAGCCAGTTCACCGAGACTGGTCATCA‑GGAAGTAAACCATCAGGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCa > 2:179041/1‑244 (MQ=255) atatatGCAGCCAGTTCACCGAGACTGGTCATCA‑GGAAGTAAACCATCAGGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTaaa > 1:599894/1‑250 (MQ=255) cAGCCAGTTCACCGAGACTGGTCATCA‑GGAAGTAAACCATCAGGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAACCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGgc > 1:262861/1‑251 (MQ=255) gccagtccaccgagctgtgtaagaa‑GGAAGAAAACCAGAAGGCCAATCAACAAATACGA‑GAGAATTCCCCCGCCGGGCCCTGCGTGAGAAATCGTTGCGCCAGAGACAAAAAAAAGACCTG‑CCCAGAGGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGgcgc < 2:454354/227‑1 (MQ=255) gCCAGTTCACCGAGACTGGTCATCA‑GGAAGTAAACCATCAGGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGgcgc > 1:43595/1‑251 (MQ=255) cAGTTCACCGAGACTGGTCATCA‑GGAAGTAAACCATCAGGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGcgcgc > 2:368668/1‑250 (MQ=255) tCACCGAGACTGGTCATCA‑GGAAGTAAACCATCAGGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGt > 1:16905/1‑251 (MQ=255) gagaCTGGTCAGCA‑GGAAAAAAACCACCAGACAAATAAGAATAT‑CGAAGAGCAACCCCCCGCCCGGCCCTGCCGGAAAAATCGTGGCGCGAGGAGCAAAAAAAAG‑CCTGGTCCCGGTGAAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAg < 2:469476/251‑1 (MQ=255) gagactggcaaacaggaaaaaacccATCAGCCCAATGAGCTTATACGA‑GAGCATTGCCCCGCCCGGACCTCCCGTAAAAATCTTTGCGCAAGAGACAAAAAAAAGCCCTG‑TACCGGGGAAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAg < 1:44391/228‑1 (MQ=255) aCTGGTCATCA‑GGAAGTAAACCATCAGGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACg > 2:246007/1‑251 (MQ=255) catcagaaagaaacccacaagccaaaTCAGCATATACGA‑GAGCAGTCCCCCCCCGGGCCCTGCATGAGAAATCGTTGCGCAAGAGACAAAAAAAAGCCCTG‑TCCCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGc < 1:751950/227‑1 (MQ=255) tca‑GGAAGTAAACCATCAGGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCa > 1:456614/1‑250 (MQ=255) aaGTAAACCATCAGGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGAGTGGCACTCCtt > 1:168095/1‑251 (MQ=255) gaaaccaatcgggcaaaTCAGCATAGACGA‑GAGCAGTCCCCCGCCCGCCCCTCCGGGAGAATTGGTGGCGCGAGAGACAAAAAAAAGCCTTG‑TCCCGATGAAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTcc < 1:751728/236‑1 (MQ=255) aaCCATCAGGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGc > 2:268067/1‑251 (MQ=255) aGGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGaa < 1:316804/93‑1 (MQ=255) aGGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGaa > 2:316804/1‑93 (MQ=255) aGCATATACGA‑GAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTtat > 1:365761/1‑251 (MQ=255) aaataaaCGA‑GAGAAATGCCCCCCCCGGCCCTGCCTGAGAAATCGTGGCGCAAGAGGAAAAAAAAAGCCCTG‑TCCCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTtata < 1:570768/245‑1 (MQ=255) atacgggggcagcgCCCCCCCCGGACCGGCCTGAAAAATCGGGGCGCAAGAAGCAAAAAAAACCCCTG‑CCCCGA‑GAAACCCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGac < 2:389696/238‑1 (MQ=255) aagagacaaaTGCCCCCCCCGGCCCTGCCTGAAAAATCGTGGCGCCAGAGCCAAAAAAAAGCCCTG‑TCCCGAGGAAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGacac < 1:805723/243‑1 (MQ=255) aaTGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCACTGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATg > 2:234596/1‑250 (MQ=255) aTGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATGAt > 1:331693/1‑251 (MQ=255) gcccccGCCCGCACCTGCCTGAGAAACGGTGGCGCCGGAGGCAAAAAAAAGCCTTG‑TCCCGATGAATCC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATGATc < 1:50920/249‑1 (MQ=255) gCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATGATCg > 1:564247/1‑251 (MQ=255) ggACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGAGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATGATCGCCCAGGGAt > 1:814289/1‑251 (MQ=255) aCCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATGATCGCCCAGGGAtt > 1:838818/1‑250 (MQ=255) ctgcctgAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATGATCGCCCAGGGATtat > 2:557288/1‑250 (MQ=255) cgccgaagaAACCGTGGCCCCAGAGGAAAAAAAAAGCCCTG‑TCCGGAGAGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATGATCGCCCAGGGATTATaa < 1:300161/245‑1 (MQ=255) ccggaaaaaTCGTGGCGCAAGAGCCAACAAAAAGCCCTG‑TCCGGATGAAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGt < 1:660581/245‑1 (MQ=255) aaaTCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGt > 1:651499/1‑118 (MQ=255) aaTCGTTGCGCCAGAGCCAACAAAAAGACCGG‑TCCCGAGGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAagcag < 1:517153/251‑1 (MQ=255) gTTGCGCCAGAGGAAACAAAACGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGCAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTACGGCAGAAATACACGGATATAAGACACCTTCATGATCGCCCAGGGCTTATCAGTAAAGCAGCCa > 2:397157/1‑250 (MQ=255) gCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTgg > 2:190607/1‑251 (MQ=255) acaaAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCg > 1:537426/1‑250 (MQ=255) | GCAACCAGGTCAACGGCGATAGTCACCGCCCAGTTGTACCAGTAGTTCCAGCCCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAAC‑GAACCGGAAACC‑GGCATATATGCAGCCAGTTCACCGAGACTGGTCATCA‑GGAAGTAAACCATCAGGCCAATCAGCATATACGA‑GAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTG‑TACCGATGGAACC‑GCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACGATTGGCAATCCTTCCGGCAGAAATACACGGTTATAAGACACCTTCATGATCGCCCAGGGATTATAAGTAAAGCAGCCAATCTCTTTTCTGGCG > NC_000913/2248186‑2248672 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |