Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,228,275	0	T	C	49.0%	27.0 / 23.6	52	intergenic (‑1005/‑707)	yohF/yohP	putative oxidoreductase YohF/putative membrane protein YohP
Reads supporting (aligned to +/- strand):  ref base T (15/11);  new base C (11/14);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 4.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAAC‑GCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTGCTCGCG‑TTCCGCC‑GCCCGTGC‑TTT‑AACCGTACCCAGGCGGGCAGTAA‑CCTCCGCCCG‑ATTC‑TTT‑CCCCAGATA‑TCGAGATGC‑CAGCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTGGTGTACCACGGG‑CCGGTCGTACCTGCGGCCGGATCGTT‑CAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCT  >  NC_000913/2228029‑2228515                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                            cGGGTTTTGCTGGCATTAATATCGGTTTCCACGCCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGAACGGGCGCGATAAGGGTGTTCTGCTCTGTTTCTATTTGCTGCAAGACCGTGTTTAAC‑GCCTCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTGCACGCG‑TTCCGCC‑GCCCGTGC‑TTT‑AACCGTACCCAGGCGGGCAGTAC‑CCTCCGc                                                                                                                                                                                                                                                         <  2:63503/251‑1 (MQ=255) cGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAAC‑GCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTGCTCGCG‑TTCCGCC‑GCCCGTGC‑TTT‑AACCGTACCCAGGCGGGCAGTAA‑CCTCCGc                                                                                                                                                                                                                                                         <  1:287786/251‑1 (MQ=255)   ggTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAAC‑GCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑GTTTCCCGCG‑GTCCGCCCGCCCCGCC‑TTT‑AACCGAACCC‑CGCGGGCCGTAA‑CCTcccccc                                                                                                                                                                                                                                                       >  2:25759/1‑251 (MQ=255)           tGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAAC‑GCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTTCTCGCG‑TTCCGCC‑GCCCGGGC‑TTT‑AACCGTACCCAGGCGGGGGGGAA‑ACCCCGCCCG‑ATTC‑ttt                                                                                                                                                                                                                                             >  1:140603/1‑251 (MQ=255)                               acacCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAAC‑GCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑GTTGCTCGCG‑TTCCGCC‑GCCCGTGC‑TTT‑AACCGTAACCCGGCGGGCAGTAAACCCCCCCCCG‑ATTT‑TTT‑CCCCaaaaacggagtgccc                                                                                                                                                                                                                         >  1:17337/1‑237 (MQ=255)                                 cccTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAAC‑GCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTGCTCGCG‑TTCCGCC‑GCCCGTGC‑TTT‑AACCGTACCCAGGCGGGTAGTAA‑CCTCCGCCCG‑ATTC‑TTT‑CCCCAGATA‑TCGAGATGC‑CAGc                                                                                                                                                                                                                    <  1:615952/250‑1 (MQ=255)                                 acCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAAC‑GCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCCACTGGCGGGGTTTCGTCG‑G‑TTCCGCC‑CCCCGCGC‑CTT‑TACCGCACCCAGGTGGGGAGCAA‑CCCCCGCCCGCATT‑‑TTT‑ccccccaaattcgtagtccacc                                                                                                                                                                                                                      >  2:494588/1‑233 (MQ=255)                                 acCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAAC‑GCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTTG‑TCGCG‑TTTCGGC‑CCCCCTGCCTTT‑AACC‑TACCCAGGCCGGGAGTAA‑ACCCCGCCCG‑TGTC‑TTT‑TCCCCGATA‑TCGGAATGC‑Caat                                                                                                                                                                                                                    >  1:691531/1‑249 (MQ=255)                                     tCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAAC‑GCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTGCTCGCG‑TTCCGCC‑GCCCGTGC‑TTT‑AACCGTACCCAGGCGGGCAGCAA‑CCTCCGCCCG‑ATTC‑TTT‑CCCCAGATA‑TCGAGATGC‑CAGccc                                                                                                                                                                                                                  <  2:345565/249‑1 (MQ=255)                                       aaCTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAAC‑GCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTGCTCCCG‑GTCCGCC‑GCCCCCGC‑TTTTAACC‑TACCCCGGCCGGCAGGAA‑CCCCCCCCCG‑GTTC‑TTT‑TCCCCGATA‑TCCAGATggccaccccccc                                                                                                                                                                                                               >  1:319984/1‑240 (MQ=255)                                                 gTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAAC‑GCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑GTTTCTCCCG‑TTTCGCC‑GCCCCGGC‑TTT‑TACCGTACCCCGGCGGGGCGTAA‑CCCCCGCCCG‑GTTT‑TTT‑TCCCCGGTA‑TTGAGATGC‑CAACCCCCC‑G‑GT‑AAggcca                                                                                                                                                                                                  >  1:697339/1‑246 (MQ=255)                                                           tCTGCTATAGCTGGCGATCGGTCGCGATAATGGTGGTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAAC‑GCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTGCTCGCG‑GTCCCCC‑GCCCCTGC‑TTTTAACCG‑CCCCCGGCGGGCCGTAA‑CCCCCCCCCC‑GTTT‑TTT‑CTCCCGATAATCG‑GGTGC‑CAGCccgcc‑ggtacggccaaaggacccgtg                                                                                                                                                                                         >  2:56978/1‑231 (MQ=255)                                                                      tGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAAC‑GCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTGCTCGCG‑TTCCCCC‑GCCCGTGC‑TTT‑AACCCTACCCCGGCGGGCAGTAA‑ACCCCCCCCC‑ATTT‑TTT‑TCCCCGCAA‑TCGGGATGC‑CCGCCCCCC‑G‑TTTAAC‑CCAAAA‑AACCCTTGGTGTACCACgg                                                                                                                                                                           >  1:554045/1‑251 (MQ=255)                                                                        gCGATGGGCCGCAATATTGGTGTTCTGCGCTTTTTCAATTGGCTTCAAGACCGTGTTTAAC‑GCCGCCTGGGTTTGCCACTCCAAGTACAGGCGGGCTACGCGGCCAGCCAGCAATTGGCGGT‑TTTGCTCGCG‑TCCCGCC‑GCCCGTGC‑TTT‑AACCGTACCCAGGCGGGCAGTAC‑CCCCCGCCCG‑ATTC‑TTT‑CCCCAGATA‑TCGAGAGGC‑CAGCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTGGTGTACCACGGG‑c                                                                                                                                                                        <  1:135620/251‑1 (MQ=255)                                                                         cGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAAC‑GCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTGTTCCCG‑GTCCGCC‑GCCCCTGC‑GTT‑TACCGTTACCACGCGGGCAGAGA‑ACTCCCCCCG‑GTTCCTTT‑CCCCAGA‑A‑TCGAGAAGCACA‑CCCGCC‑GGTT‑AGG‑CCaaaatatcgtttggggatcacacggc                                                                                                                                                                         >  2:388568/1‑229 (MQ=255)                                                                             cGGTCGCGATAAGGGTGTTCTGCTTTTTTTCTATTTGCTCCAAGACCGTGTTTAAC‑CCCGCCGGGGTTTGCCACTCCCAGTACGGGCGGGCTACGCGGCAGGCCGGAAATTGGCGGG‑TTTGCCCGGG‑TTCCGCC‑GCCCGTGC‑TTT‑ACCCGTACCCGGGCGGGCAGTAC‑CCCCCGCCCG‑ATTC‑TTT‑CCCCAGATA‑TCGAGATGC‑CAGCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTGGTGTACCACGGG‑CCGGTc                                                                                                                                                                   <  1:335515/251‑1 (MQ=255)                                                                                    gATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAAC‑GCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTGCTCGCG‑GTCCGCC‑GCCCCGGC‑TTT‑AACCCTACCCAGGCGGGCCGTAA‑ACTCCGCCCG‑ATTC‑CTT‑TCCCAGATA‑TCGAGATGC‑CAGCCCGCC‑G‑TT‑TAG‑CCAAAAATACCGTTGGGGTACCACGGG‑CCGGGCGTAccct                                                                                                                                                            >  1:457590/1‑249 (MQ=255)                                                                                         gggtgttcttgttttttcctttttGCTGAAAGCCCGTGTTTACC‑CCACCCTGGGTTTGCCACCCCCAGTAAGGGCGGGTGACGCTGCCGGCCAGCATTGGGCGGG‑TTTGCTCGCT‑TTCCGCG‑GCCCGTGC‑TTT‑ACCCGTCCCCAGGCGGGAATTAC‑CCTCCGCCGG‑ATTC‑TTC‑CCCCAGATA‑TCGAGATGC‑CAGCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTGGTGTACCACGGG‑CCGGTCGTACCTGCGGcc                                                                                                                                                       <  2:212393/230‑1 (MQ=255)                                                                                         gggTGTTCTCCTTTTTTTCTTTTTGC‑GAAAAGCCCTGTTTAACCGCCGCCGGGGTTTGCCCCCCCCAGTCCAGGGGGGCTACGCGGCCGGCCAGCAATTGGCGGT‑TTTGCTCGCT‑TTCCGCC‑CCCCGTTT‑TTT‑ACCCGACCCCAGGGGGGCAGTAC‑CCCCCCCCCG‑ATTC‑TTC‑CCCCAGATA‑TCGAGATGC‑CAGCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTGGTGTACCACGGG‑CCGGTCGTACCTGCGGcc                                                                                                                                                       <  2:697339/250‑1 (MQ=255)                                                                                               ccttctctTTTTCTATTTGCTGCAAGCCCTTTTTTAAC‑CCCGCCGGGGTTTGCCACTCCCAGTACGGGCGGTCTACGCTGCCAGCCAGCAATTGGCGGT‑TTTGCTCGCG‑TTCCGCC‑CCCCGTGC‑TTT‑AACCGTCCCCGGGCGGGCAGTAA‑CCCCCCCCCG‑CTTC‑TTT‑CCCCAGATA‑TCGATATCC‑CAGCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTGGTGTACCACGGG‑CCGGTCGTACCTGCGGCCGGATc                                                                                                                                                  <  1:357234/246‑1 (MQ=255)                                                                                                  gttctTTTCCTATTGCCTGCAAGACCGGTTTTAAC‑GCCGCCTGGTTTTGCCCCTCCCGGGACGGGCGGGTTCCGCTGCCAGCCGGCAATTGGCGGG‑TTTGCTCGCT‑TTCCGCC‑GCCCGTGC‑TTT‑AACCTTCCCCAGGGGGGCAGTAC‑CCCCCGCCCA‑TTTC‑TTT‑CCCCAGATA‑TCGAGATGC‑CAGCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTGGTGTACCACGGG‑CCGGTCGTACCTGCGGCCGGATCGt                                                                                                                                                <  1:201857/247‑1 (MQ=255)                                                                                                  cctctTTTTCTATTCGCTGCAAGCCCGTGTTTACC‑GCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTCCGCTGCCGGCCAGCAATTGGGGGG‑TTGGCTCGGT‑TTCCGCC‑GCCTGCGC‑TTT‑ACCCGTACCAAGGCGGCAAGTAC‑CCCCCCCCGG‑ATTT‑TTC‑CCCCAGATA‑TCGAGATGC‑AAGCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTGGTGTACCACGGG‑CCGGTCGTACCTGCGGCCGGATCGTT‑c                                                                                                                                             <  1:42854/250‑1 (MQ=255)                                                                                                           cTATTTGCTGCAAGACCGTGTTTAAC‑GCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGACTACGCTGCCAGCCAGCAATTGGCGGG‑GATGCCCGCG‑CTCCGCC‑CCCCGGGC‑TTT‑TACCCGACCCAGGGGGGCCGTAA‑CCGCCCCCCC‑AATC‑TTTTCCCCA‑ACA‑TCGAGAAGG‑CAACCCGCCCG‑TA‑AAG‑C‑AAAAATAACGTTTGGGGACCACCGG‑CCGGGCGGAACCGCGGCCGGATCGCT‑CAGGGCAAAc                                                                                                                                    >  1:60802/1‑251 (MQ=255)                                                                                                               ttGCTGCAAGACCGTGTTTAAC‑GCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑GTTTCTCGCG‑TTCCGCC‑GCCCGTGC‑TTT‑AAACGGACCCAGGCGGGCAGTAA‑CCTCCGCCCG‑CTTC‑TTT‑TCCCCGATA‑TCCAGATGC‑CAACCCCCC‑G‑GT‑AAG‑CCAAAAAGACCGTTGGTGTAACACCGG‑CCCGGCGTAACTGCGGCCgggtacatcagagccaaaggcc                                                                                                                                 >  1:641248/1‑231 (MQ=255)                                                                                                                ggcggcAAGACCGTGTTTAAC‑GCCGCCGGGGTTTGCCACCCCCAGTACAGGCGGGCTACGTTGCCAGCCAGCAATTGGCGGG‑TTTGCTCGCG‑TTCCGCC‑GCCCGTGT‑TTT‑ACCCGTACCCGGGGGGGCAGTAC‑CCTCCGCCCG‑TTTC‑TTT‑CCCCAGATA‑TCGAGATGC‑CAGCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTGGTGTACCACGGG‑CCGGTCGTACCTGCGGCCGGATCGTT‑CAGAGCAAACGGccc                                                                                                                               <  1:29404/247‑1 (MQ=255)                                                                                                                   tgcAAGACCGTGTTTAAC‑GCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTTCTCGCG‑GTCCGCC‑CCCCGGGC‑TTT‑AACCGTACCCAGGCGGGCAGTAA‑CCCCCGCCCC‑CTTC‑TTT‑CCCCCGATA‑TCGAGAATC‑CAACCCGCC‑G‑TT‑AAA‑CCAAAAGTACCGTTGGTGTACCACGGG‑CCGGTCGTACCTGCGGCCGGAACGGT‑CCGGGCAAACGGccccct                                                                                                                            >  1:148485/1‑249 (MQ=255)                                                                                                                    gcAAGACCGTGTTTAAC‑GCCGCCTGGGTTTGCCCCTCCCAGTACAGGCGGGCTACGCGGCCAGCCAGCAATTGGCGGG‑TTTGCTCGCG‑TTCCGCC‑GCCCGTGC‑TTT‑ACCCGTCCCCAGGCGGGCAGTAC‑CCCCCGCCCG‑ATTC‑TTT‑CCCCAGATA‑TCGAGATGC‑CAGCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTGGTGTACCACGGG‑CCGGTCGTACCTGCGGCCGGATCGTT‑CAGAGCAAACGGCCCCAtt                                                                                                                           <  1:365562/251‑1 (MQ=255)                                                                                                                                       ccgccTGGTTTTGCCACTCCCAGTAAAGGCGGGCTACGCTGCCGGCAGGCAATTGGCGGT‑TTTGCTCGCG‑TTCCGCC‑GCCCGTGC‑TTA‑AACCGTACCCAGGCGGGCAGTAC‑CCTCCGCCCG‑ATTT‑TTT‑CCCCAGATA‑TCGAGATGC‑CAGCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTGGTGTACCACGGG‑CCGGTCGTACCTGCGGCCGGATCGTT‑CAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACAtt                                                                                                         <  1:59508/251‑1 (MQ=255)                                                                                                                                       ccgccTGGGTTTGCCCCTCCCATAACGGGGGGGCTCCGCGGCCAGCCGGCAATGGGCGGG‑TTTGCTCGCG‑TTCCGCC‑GCCCGTGC‑TTT‑ACCCGTACCCAGGCGGGCAGTAA‑CCTCCGCCCG‑ATTC‑TTC‑CCCAAGAAA‑TCGAGATGC‑AAGCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTGGTGTACCACGGG‑CCGGTCGTACCTGCGGCCGGATCGTT‑CAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACAtt                                                                                                         <  1:35938/251‑1 (MQ=255)                                                                                                                                         gcctggttttccctcgcccAGTACGGGCGGGCTACGCTGCCAGCCAGAAATGGGCGGT‑TTTGCCCGCG‑TTCCGCC‑GCCCGTGC‑TTT‑ACCCGTCCCCAGGCGGGCATTAC‑CCCCCGCCCG‑TTTC‑TTT‑CCCCAGATA‑TCGAGATGC‑CGGCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTGGTGTACCACGGG‑CCGGTCGTACCTGCGGCCGGATCGTT‑CAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACAtttt                                                                                                       <  1:76595/235‑1 (MQ=255)                                                                                                                                          ccTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑GTTGCTCCCG‑GTCCGCC‑GCCCGGGC‑TTT‑AAACGTAACCAGGGGGGGCGTAA‑AATCCGCCCG‑ATTC‑TTT‑TCCCCGATA‑TCGAGATGG‑CAACCCCCC‑G‑TT‑AAG‑CCAAAAATACCGTTGGGGTTCCCCGGG‑GCGGGCGTACCCTCGGCCCGGGCGTT‑CATAGCGAACAGCCCCacttaaccctcttccggccttttt                                                                                                      >  1:589573/1‑228 (MQ=255)                                                                                                                                           cTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTTCTCCCC‑TTCCGCC‑CCCCGTGC‑TTT‑AACCGTACCCCAGCGGGCAGTAA‑CCTCCGCCCG‑CTTT‑TTT‑CCCCAGATA‑TCGACATGC‑CAGCCCGCC‑G‑GT‑AAGACCAAAA‑TACCGTTGGGGGGCAACGGG‑GCGGGCGGACATGCGGCCCGATCGTTACA‑AGCAAACCGACCCACTAAGACTTCTGcccccattcttt                                                                                                     >  2:545328/1‑241 (MQ=255)                                                                                                                                            ggggTTTGCCCCCCCCAGTACGGGCGGGCTCCGCTGCCAGCCAGCAATGGGCGGG‑TTTGCTCGCG‑TTCCGCC‑GCCCGTGC‑TTA‑AACCGTCCCCAGGCGGGCAGTAC‑CCTCCGCCCG‑ATTC‑TTT‑CCCCAGATA‑TCGAGATGC‑CAGCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTGGTGTACCACGGG‑CCGGTCGTACCTGCGGCCGGATCGTT‑CAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACAtttttt                                                                                                     <  1:635768/249‑1 (MQ=255)                                                                                                                                             gggTTTGCCACTCCCAGAACAGGCGGGCTACGCTGCCAGCCGGAAAGTGGCGGG‑TTTGCTCGCG‑TTCCCCC‑GCCGGTTT‑TTT‑AACCGTCCCCAGGCGGGCAGTAC‑CCTCCGCCCG‑ATTT‑TTT‑CCCCAGATA‑TCGAGATGC‑CAGCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTGGTGTACCACGGG‑CCGGTCGTACCTGCGGCCGGATCGTT‑CAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGc                                                                                                   <  1:592471/251‑1 (MQ=255)                                                                                                                                              ggTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑GTTTCTCGCGGTTCC‑CC‑GCCCCTGC‑TTTTAACC‑TACCCAGGCGGGGAGTAA‑ACCCCGCCCC‑ATTT‑TTT‑TCCCCGATA‑TCGAGGTGC‑CCGCCCCCC‑G‑TT‑AAG‑CCAAAAGTACCGTTTGGGGACCACGGGGCCGG‑CGTACCCTCGGCCGGATCGGT‑CCGAGGAAAAGGCCCCCTTTAGCCTTCTGCCGACCtttttttcc                                                                                                  >  1:452747/1‑248 (MQ=255)                                                                                                                                                                  ggcgggcctccgcgccagcccGAAATTTGCGGG‑TTTGCTCGCG‑TCCCCCC‑CCCCGTTC‑TTT‑ACCCGTCCCCAGGGGGGCAGTAA‑CCCCCCCCCG‑ATTT‑TTT‑CCCCAGATA‑TCGAGATGC‑CAGCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTGGTGTACCACGGG‑CCGGTCGTACCTGCGGCCGGATCGTT‑CAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAg                                                                              <  1:77817/238‑1 (MQ=255)                                                                                                                                                                        cTACGCTGCCAGCCAGCAATTGGCGGG‑TTTGCTCGCG‑TTCCGCC‑GCCCGTGC‑TTT‑AACCGTACCCAGGCGGGCAGTAA‑CCTCCGCCCG‑ATTC‑TTT‑CCCCAGATA‑TCGAGATGC‑CAGCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTGGTGTACCACGGG‑CCGGTCGGACCTGCGGCCGGATCGGT‑CAGAGCAAACGGCCCCCTTAAACCTTCTGCCGACATTTTTTTCCGCTCCATATCCGCCGAAAAGTCGATc                                                                        >  1:799487/1‑251 (MQ=255)                                                                                                                                                                               gccagccaGCAATTGGCGGG‑TTTGCTCGCG‑GTCCGCC‑GCCCGTGC‑TTT‑AACCGTACCCAGGCGGGCAGTAA‑CCCCCGCCCG‑ATTC‑TTT‑CCCCAGATA‑TCGAGATGC‑CAGCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTGGTGTACCACGGG‑CCGGTCGTACCTGCGGCCGGAACGTT‑CAGAGCAAACGGCCCCCTTAAGCCTTCTTCCGACATTTTTTGCCGCCCCATATCCGCCCAAAAGTCGATCTGCGGAc                                                                 >  1:528133/1‑251 (MQ=255)                                                                                                                                                                                 cagccagcAATTGGGGGG‑TTTGCGCGCG‑TTCCGCC‑GCCCGGGT‑TTT‑AACCGTCCCCAGGCGGGCAGTAA‑CCTCCGCCCG‑ATTC‑TTC‑CCCCAGATA‑TCGAGATGC‑CAGCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTGGTGTACCAc                                                                                                                                                                             <  1:572037/143‑1 (MQ=255)                                                                                                                                                                                 cagccagcAATTGGCGGG‑TTTGCTCGCG‑TTCCGCC‑GCCCGTGC‑TTT‑AACCGTACCCAGGCGGGCAGTAA‑ACTCCGCCCC‑ATTC‑TTT‑CCCCAGATA‑TCGCGATGC‑CCGCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTTGTGTACCAc                                                                                                                                                                             >  2:572037/1‑143 (MQ=255)                                                                                                                                                                                         aaTTGGCGGG‑TTTGCTCGCG‑TTCCGCC‑CCCCCTGC‑TTT‑AACCGTACCCAGGCGGGCCGCAA‑CCTCCGCCCG‑ATTC‑TTT‑TCCCCGATT‑TCGAGATGC‑CAGCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTGGTGTACCACGGG‑CCGGTCGTACCTGCGGCCCGATCGTT‑CAGAGCAAACGGCCCCATTAAGCCCTCTGCCGCCATTTTTTGCCGCTCCATCTCCGCCGAAAAGTCGCACTGCGGCCCAGCCtcagg                                                       >  2:207701/1‑247 (MQ=255)                                                                                                                                                                                               cGGG‑TTTGCTGGCG‑TCCCCCC‑GCCCGTGT‑TTA‑AACCGTCCCCCGGCGGGAAGTAC‑CCCCCCCCCG‑ATTC‑TTC‑CCCCAGATA‑TGGAGACGC‑CACCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTGGTGTACCACGGG‑CCGGTCGTACCTGCGGCCGGATCGTT‑CAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAAc                                                 <  1:460817/251‑1 (MQ=255)                                                                                                                                                                                                ggg‑TTTGCTCGCG‑TTCCGCC‑GCCCGTGC‑TTT‑AACCGTACCCAGGCGGGCAGTAA‑CCTCCGCCCG‑ATTC‑TTT‑CCCCAGATA‑TCGAGATGC‑CAGCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTGGTGTACCACGGG‑CCGGTCGTACCTGCGGCCGGATCGTT‑CAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTg                                                      >  1:483392/1‑245 (MQ=255)                                                                                                                                                                                                ggg‑TTTGCTCGCG‑TTCCGCC‑GCCCGTGC‑TTT‑AACCGTACCCAGGCGGGCAGTAA‑CCTCCGCCCG‑ATTC‑TTT‑CCCCAGATA‑TCGAGATGC‑CAGCCCGCC‑G‑TT‑AAA‑CCCAAAGTACCGTTGGTGTACCACGGG‑CCGGTCGTACCTGCGGCCGGACCGTT‑CAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACCTTTTTTGCCGCTCCATATCCGCCGCAAAGTCGATCTGCGGACCACCCTGAGTGGctacc                                                >  2:611002/1‑247 (MQ=255)                                                                                                                                                                                                                              gtttt‑ACCCGTCCCCAGGCGGGCAGTAC‑CCCCCCCCCG‑ATTC‑TTC‑CCCCAAATA‑TCGAGTTGC‑CAGCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTGGTGTACCACGGG‑CCGGTCGTACCTGCGGCCGGATCGTT‑CAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCagctagct                       <  1:547229/248‑1 (MQ=255)                                                                                                                                                                                                                                ttt‑AACCGACCCCAGGGGGGGAGTAC‑CCCCCCCCGG‑ATTC‑TTT‑CCCCAGATA‑TCGAGATGC‑CGGCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTGGTGTACCACGGG‑CCGGTCGTACCTGCGGCCGGATCGTT‑CAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAgctggctg                      <  1:850686/249‑1 (MQ=255)                                                                                                                                                                                                                                   tacccGTACCCGGGCGGGCAGTAC‑CCCCCCCCCT‑ATTC‑TTC‑CCCCAGATA‑TCGAGATGC‑CCGCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTGGTGTACCACGGG‑CCGGTCGTACCTGCGGCCGGATCGTT‑CAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAAt                   <  1:593805/247‑1 (MQ=255)                                                                                                                                                                                                                                        gTACCCAGGCGGGCAGTAA‑CCTCCGCCCG‑ATTC‑TTT‑CCCCAGATA‑TCGAGATGC‑CAGCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTGGTGTACCACGGG‑CCGGTCGTACCTGCGGCCGGATCGTT‑CAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCagcta                          >  1:850807/1‑238 (MQ=255)                                                                                                                                                                                                                                         cccccAGGCGGGCAGTAC‑CCCCCGCCCG‑ATTC‑TTC‑CCCCAAATA‑TCGAGTTGC‑CAGCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTGGTGTACCACGGG‑CCGGTCGTACCTGCGGCCGGATCGTT‑CAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGt            <  1:25759/249‑1 (MQ=255)                                                                                                                                                                                                                                             cAGGCGGGCAGTAA‑CCTCCGCCCG‑ATTC‑TTT‑CCCCAGATA‑TCGAGATGC‑CAGCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTGGTGTACCACGGG‑CCGGTCGTACCTGCGGCCGGCTCGTT‑CAGAGCAAACGGCCCCATTACGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTc          >  2:606535/1‑249 (MQ=255)                                                                                                                                                                                                                                                gCGGGCAGTAA‑CCTCCGCCCG‑ATTC‑TTT‑CCCCAGATA‑TCGAGATGC‑CAGCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTGGTGTACCACGGG‑CCGGTCGTACCTGCGGCCGGATCGTT‑CAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGcc      >  2:284716/1‑250 (MQ=255)                                                                                                                                                                                                                                                   ggCAGTAA‑CCTCCGCCCG‑ATTC‑TTT‑CCCCAGATA‑TCGAGATGC‑CAGCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTGGTGTACCACGGG‑CCGGTCGTACCTGCGGCCGGATCGTT‑CAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCt  >  1:638723/1‑251 (MQ=255)                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                            CGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAAC‑GCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGG‑TTTGCTCGCG‑TTCCGCC‑GCCCGTGC‑TTT‑AACCGTACCCAGGCGGGCAGTAA‑CCTCCGCCCG‑ATTC‑TTT‑CCCCAGATA‑TCGAGATGC‑CAGCCCGCC‑G‑TT‑AAG‑CCAAAAGTACCGTTGGTGTACCACGGG‑CCGGTCGTACCTGCGGCCGGATCGTT‑CAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCT  >  NC_000913/2228029‑2228515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑