Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,301,917	C→T	Q246Y (CAG→TAT)	oppA →	oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,301,917	0	C	T	100.0%	221.9 / NA	61	Q246Y (CAG→TAT)	oppA	oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/30);  total (31/30)

GATCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATAT  >  NC_000913/1301680‑1302161                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     gatCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt                                                                                                                                                                                                                                          <  2:114204/250‑1 (MQ=255)        caTTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAATGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAtt                                                                                                                                                                                                                                  <  2:167972/251‑1 (MQ=255)                  ccccTTAAGTGAACCCGTTCCGTACTTCTATAAATTTCTTGTTCACCCATCAACTTCACCGGTGCCAAAATCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAg                                                                                                                                                                                                                        <  2:421362/249‑1 (MQ=255)                       tAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                                                                                                                                                                                                                   >  1:222226/1‑251 (MQ=255)                       tAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAc                                                                                                                                                                                                                    <  2:185900/250‑1 (MQ=255)                        aaGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCg                                                                                                                                                                                                                  >  2:90090/1‑251 (MQ=255)                             aaCCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc                                                                                                                                                                                                             >  1:371831/1‑251 (MQ=255)                                ccGTTCCGTACTTCTATAAATTACTTGTTCACCGATCAACTTCACCGGTGCCAAACGCCGCTATCGAGAAATTCGGCGACAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTAAACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTCCCGATGTCAAc                                                                                                                                                                                                          >  1:326924/1‑251 (MQ=255)                                           ttCTATAAGTTACTTGTTCACCCATCAACTTTACCGGTTCCAATAGCCGCTATCGAGAAATTCGGCGACAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAACCCGTAATAAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGtgg                                                                                                                                                                                               >  2:154251/1‑249 (MQ=255)                                               ataAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAgtggg                                                                                                                                                                                           >  1:307921/1‑250 (MQ=255)                                               ataAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCAGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAACTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAgtggt                                                                                                                                                                                           >  2:32948/1‑251 (MQ=255)                                                   aTTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTTGTGaaa                                                                                                                                                                                       <  2:320166/251‑1 (MQ=255)                                                   aTTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGaa                                                                                                                                                                                        >  1:315060/1‑250 (MQ=255)                                                             cACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGAc                                                                                                                                                                             >  1:291268/1‑251 (MQ=255)                                                                                 gCCAAAAGCCGCTATCGAGAGATTCGGCGAAAAATGGACCCAGCCTGGTAATATCTTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGTAGCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAATTTGCCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATc                                                                                                                                                          <  1:152270/250‑1 (MQ=255)                                                                                      aagcagctaaagagaAATTCGACGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACAAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAGCTATTTGCCTATTGCTTCTGAAGTTACCGAAGTCAACCGCTACCGTAGTGGTGAAAGCTACAAGAAGAATAACAGCATGCCGATCGAATTg                                                                                                                                                    <  2:83284/240‑1 (MQ=255)                                                                                       aGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGt                                                                                                                                                   <  1:90090/251‑1 (MQ=255)                                                                                        gCCGCTATCGATAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTAGACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCATGAACGCAGCCCTACCTACTGGAACAACGCGAAAACCGTTATTAACTTTGTAACCGATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTTCCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGAGCAAATTGtt                                                                                                                                                  <  2:296218/251‑1 (MQ=255)                                                                                         ccGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATTCCGTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCAGGCCGATCGAATTGttg                                                                                                                                                 <  2:412130/251‑2 (MQ=255)                                                                                              aTCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAAGCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTGCCAGa                                                                                                                                             <  1:414107/250‑1 (MQ=255)                                                                                                      aTTCGGAGTAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTACAGAAGCTGaaa                                                                                                                                     >  2:386879/1‑250 (MQ=255)                                                                                                          ggCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAaga                                                                                                                                <  2:98206/251‑1 (MQ=255)                                                                                                           gctaaaaaTGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGGCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGAGCGAATTGTTCCAGAAGCTGAAAAAagag                                                                                                                               <  2:94995/248‑1 (MQ=255)                                                                                                           gCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAagag                                                                                                                               <  1:303142/251‑1 (MQ=255)                                                                                                            cGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAgaga                                                                                                                              <  2:231764/251‑1 (MQ=255)                                                                                                                        ccAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGa                                                                                                                    >  2:313216/1‑249 (MQ=255)                                                                                                                               ggTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGACTTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGACGTTCACGt                                                                                                           >  2:199362/1‑251 (MQ=255)                                                                                                                                gTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACTCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGtt                                                                                                          <  2:401095/251‑1 (MQ=255)                                                                                                                                gTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGtt                                                                                                          <  2:307921/251‑1 (MQ=255)                                                                                                                                  aaTATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCAGGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTg                                                                                                         <  2:139175/250‑1 (MQ=255)                                                                                                                                   atatCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGt                                                                                                                                                   <  2:328034/207‑1 (MQ=255)                                                                                                                                   atatCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGt                                                                                                                                                   >  1:328034/1‑207 (MQ=255)                                                                                                                                       cGTCACCAACGGCGCCTATACCTTAAAAGATTGAGTCGTAAGCGAACGAATCGTTCTTGAACGCAGCCCGACCGACTGGAACAACCCGAAAGCCGTTATTGACTATGTAACCTATTTGCCTAGTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATCACGAATAACAGTATGCCGATCGAATTGTTGCACAATATGAAAAAAGAGATTCCGGACGAAATTCCGGTTGATCCAt                                                                                                   <  2:164650/251‑1 (MQ=255)                                                                                                                                        gTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCAt                                                                                                   >  1:392190/1‑250 (MQ=255)                                                                                                                                        gTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCAt                                                                                                   >  2:71262/1‑250 (MQ=255)                                                                                                                                              aaCGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCtgtg                                                                                            <  1:2764/251‑1 (MQ=255)                                                                                                                                                cGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGc                                                                                           >  2:115736/1‑250 (MQ=255)                                                                                                                                                        ataCATTAAAAGATTGGGTCGTAACCGAACGCATCGTTCTTAAACGCAGCACGACCTACTGGAACAACGCGAAAACCGTTCTTAAATATGTAACCTCTTTGCCTATTGCTTCTGAAGTTACCGATGTCAAACGCTACCGTAGTGGTGCAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGACAAAAGAGATCCCTGACGAAGTTAACGTTGTTCCATACCTGTGCACTTACTAt                                                                                  >  2:3470/1‑251 (MQ=255)                                                                                                                                                               aaaaGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAagag                                                                                                                               <  1:49387/199‑1 (MQ=255)                                                                                                                                                               aaaaGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAagag                                                                                                                               >  2:49387/1‑199 (MQ=255)                                                                                                                                                               aaaaGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGaa                                                                            <  2:302692/250‑1 (MQ=255)                                                                                                                                                                       gggTCGTAAACGAACGAAGCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTaacaa                                                                    <  1:213604/250‑1 (MQ=255)                                                                                                                                                                              aaaCGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAAc                                                            >  1:20529/1‑251 (MQ=255)                                                                                                                                                                                  gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGCTGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCCGAAGCTGAAAAAAGAGATCCCGGCCGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGcc                                                        >  2:38899/1‑251 (MQ=255)                                                                                                                                                                                  gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGcc                                                        >  1:225689/1‑251 (MQ=255)                                                                                                                                                                                   aacgaaTTGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGCAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGCAAAAAGAGATCCCGGAAGAAGTTCACGTTGATCCATACCTGTGAACTTACTATTACGAAATTAACAACCAGAAACCGcc                                                        >  2:307383/1‑250 (MQ=255)                                                                                                                                                                                   aacgaaTCGTTCTTGAACGCAGCCCGACATACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACAGcc                                                        >  1:307565/1‑250 (MQ=255)                                                                                                                                                                                         cctttCTTTAACGCTGCCCGACCTCCTGGAACAACGCGAAACCCTTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATTTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATCCCTGTGCACTTACTATTACGAAATTAACACCCAGAAACCGCCATTCAAc                                                 <  2:227266/248‑1 (MQ=255)                                                                                                                                                                                          cGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAAACGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGCGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACg                                                >  1:112258/1‑251 (MQ=255)                                                                                                                                                                                                      cAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTCCTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcgtgtgc                                    <  2:350643/251‑1 (MQ=255)                                                                                                                                                                                                        gCCCGACCTACTGAAACAACGCGAAAACCGTTATGACCTATGTAACCAATTTGCCTATTACTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAAAAGCTGAAAAAAGATATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTTCTATTACGAAATTAACACCCAGAAACCGCCATTCAACGAtgtgcgtgtgcgg                                  <  2:86818/251‑2 (MQ=255)                                                                                                                                                                                                         cccGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATgtgcgtgtgcgt                                  >  2:198772/1‑250 (MQ=255)                                                                                                                                                                                                             aCCTACTGCAACAATGCGAAAACCGTTATTAACTATGGATCCTATTTGACTATTGCTTTTGAAGTTACCGAGGTCAACCGCTACCGTTGTGTTGAAATCGACATGACGAATAATAGCATGCCGATCGAATTGTTCCAAAAGCTAAAAAAAGAAATCCCGGACGAAGTTCCCGTTGATCCATACCGGTGCACGTACTATATCGAAATTATCAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGc                             <  1:330731/251‑1 (MQ=255)                                                                                                                                                                                                                 aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACt                          <  1:51522/250‑1 (MQ=255)                                                                                                                                                                                                                     gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAAc                     >  1:223532/1‑251 (MQ=255)                                                                                                                                                                                                                       acaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTaacaac                                                                   >  1:232291/1‑203 (MQ=255)                                                                                                                                                                                                                       acaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTaacaac                                                                   <  2:232291/203‑1 (MQ=255)                                                                                                                                                                                                                               aaaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATgg            >  1:219284/1‑250 (MQ=255)                                                                                                                                                                                                                                aaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGAc          >  1:264736/1‑251 (MQ=255)                                                                                                                                                                                                                                      ttattaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGat    >  2:268877/1‑251 (MQ=255)                                                                                                                                                                                                                                        attaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGatat  >  2:118791/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     GATCACACATTAGAAGTCACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATAT  >  NC_000913/1301680‑1302161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑