Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	670,635	A→T	L324Q (CTG→CAG)	holA ←	DNA polymerase III subunit delta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	670,635	0	A	T	100.0%	203.4 / NA	52	L324Q (CTG→CAG)	holA	DNA polymerase III subunit delta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (23/29);  total (23/29)

AGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCAGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAACAACAGTTCACGTTGTAATGTGCGCA  >  NC_000913/670388‑670866                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                        agatCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACAAGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGCTTTAGATGACCATAGTGCACCGGATCGAAGGCGCCGCCACACAGAGACTGTAAAGATTTCATATCAACCGTCGTTAAATACGTCCGCCAGGGGTTTATGGCACAACAGCAGAGATAACCCTTCCTGct                                                                                                                                                                                                                                      >  2:339379/4‑251 (MQ=255) aGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGct                                                                                                                                                                                                                                      <  2:78149/251‑1 (MQ=255)  gTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGctc                                                                                                                                                                                                                                     <  2:260453/251‑1 (MQ=255)            gtgtTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACact                                                                                                                                                                                                                           >  1:301577/1‑251 (MQ=255)                ttACGCTTCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACactgac                                                                                                                                                                                                                        <  1:128785/250‑1 (MQ=255)                  aCGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACactgactg                                                                                                                                                                                                                      <  1:127762/250‑1 (MQ=255)                   cGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACtgactgac                                                                                                                                                                                                                    <  2:47509/251‑1 (MQ=255)                        cACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTaa                                                                                                                                                                                                               <  2:201912/251‑1 (MQ=255)                         aCGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAAt                                                                                                                                                                                                              <  1:349593/251‑1 (MQ=255)                                                 cGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTc                                                                                                                                                                                      <  1:153181/251‑1 (MQ=255)                                                            aaCATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGt                                                                                                                                                                           >  1:150456/1‑251 (MQ=255)                                                                ttattaGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGc                                                                                                                                                                        >  2:376337/1‑250 (MQ=255)                                                                  attaGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCAc                                                                                                                                                                      <  1:398706/250‑1 (MQ=255)                                                                  attaGGGATGATTGTGACCCGCGTCAGACCAATCAAATGCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCAc                                                                                                                                                                      <  2:254298/250‑1 (MQ=255)                                                                   ttaGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACgg                                                                                                                                                                    >  1:389993/1‑251 (MQ=255)                                                                    taGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACgg                                                                                                                                                                    <  1:78507/250‑1 (MQ=255)                                                                         atgatTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGAc                                                                                                                                                              <  1:140802/251‑1 (MQ=255)                                                                                        gTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGAc                                                                                                                                               <  2:134082/251‑1 (MQ=255)                                                                                          cAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACtt                                                                                                                                             >  1:92379/1‑251 (MQ=255)                                                                                              ccAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGc                                                                                                                                         <  1:40224/251‑1 (MQ=255)                                                                                                     aaTTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTa                                                                                                                                   >  1:227829/1‑250 (MQ=255)                                                                                                           ccggCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATGAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTAGGGCACAACAGAAGAGATATCCCGTCCTGCTCTGCCCACACTGACTGACCGTAATCTTTTTTGAGGGTGAGTTCCGTTCGTGTCAGTAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTc                                                                                                                            <  2:412074/248‑1 (MQ=255)                                                                                                                  ttCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCcat                                                                                                                      <  2:144706/250‑1 (MQ=255)                                                                                                                     cACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATg                                                                                                                  <  1:118888/251‑1 (MQ=255)                                                                                                                                   gACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTg                                                                                                    >  1:230345/1‑251 (MQ=255)                                                                                                                                          gTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATAcc                                                                                             <  2:109286/251‑1 (MQ=255)                                                                                                                                           tGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCCCAACAGAAGAGCTAACCCTTCCTGCTCTGCACACACTGACTGACCGTAATCTTGCTAGAGGGTGAGTTCCGTTCGCGTCAGGAGTTGCACGGCCTGACGTAACTGCGCCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATagcc                                                                                            >  2:286599/1‑248 (MQ=255)                                                                                                                                            gCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAATATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCg                                                                                           <  1:124777/251‑1 (MQ=255)                                                                                                                                                cGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGc                                                                                       >  1:101479/1‑251 (MQ=255)                                                                                                                                                            gccgccAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCCTATCAaacaac                                                                           >  1:353510/1‑251 (MQ=255)                                                                                                                                                                ccAATCAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTATCGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAacgcac                                                                       <  1:319/251‑1 (MQ=255)                                                                                                                                                                          ccTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGt                                                             >  1:332874/1‑251 (MQ=255)                                                                                                                                                                           cTGTAAAGCTTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGGTTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCCTACCCGACGCTTATCCACCAACGCCCGCAGTGGCGt                                                             >  2:305306/1‑250 (MQ=255)                                                                                                                                                                               aaaGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATggg                                                        <  1:22839/251‑1 (MQ=255)                                                                                                                                                                                  gATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACCACGCCCGCAGTGGCGTATGGGc                                                       >  2:111812/1‑249 (MQ=255)                                                                                                                                                                                     ttCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACt                                                  >  1:38769/1‑251 (MQ=255)                                                                                                                                                                                            cAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGttt                                           <  1:315694/251‑1 (MQ=255)                                                                                                                                                                                                     aTAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAAc                                  <  1:285822/251‑1 (MQ=255)                                                                                                                                                                                                       aaaTACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCACCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAAcc                                 >  1:117126/1‑250 (MQ=255)                                                                                                                                                                                                       aaaTACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGACCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCCGTGGCGTATGGGCAGACTGGCGTTTCAGGTTaaac                                 >  2:226108/1‑248 (MQ=255)                                                                                                                                                                                                         aaccttCCGCCAGGGGTTTATTGCACAACAGAATAGATAACCCTTCCTGCTCGGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGGGTTCCGTTCGTGTCAGGAGTTGCACGCCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCAGCATGCCCCGGCGGTTCTGCCATACCCGAGGCTTATCATACAACGCACGCAGTGGCGTATGGGCAGACTCGCGTTTCAGGTGAACCAGt                              <  2:360831/246‑1 (MQ=255)                                                                                                                                                                                                             gTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTaaca                          <  1:224105/251‑1 (MQ=255)                                                                                                                                                                                                                  ccAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCCATGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAacaacaat                     >  1:23111/1‑249 (MQ=255)                                                                                                                                                                                                                    aGGGGTTTATGGCGCAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGTGTTCCGTTCGTGGCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGCTGTTTGTCAAACAACGCACGCAGCGGCGTATGGGCAGACTGGCGTTTCAGGTTAAcaagaaaacaaagtgc                   >  2:58001/1‑236 (MQ=255)                                                                                                                                                                                                                    aGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAACAACAGTTc                   <  1:85664/251‑1 (MQ=255)                                                                                                                                                                                                                       ggTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAACAACAGTTCACg                >  2:38502/1‑251 (MQ=255)                                                                                                                                                                                                                          ttATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAACAACAGTTCACGtt              <  1:97869/250‑1 (MQ=255)                                                                                                                                                                                                                           caTGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGCTGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACCGGCGTTTCAGGCTAACCAGTAACAACAGTTCACGTTGt            >  2:36253/2‑251 (MQ=255)                                                                                                                                                                                                                            aTGGCACAACAGAAGAGATAACCCTGCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGCCTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACGGGCGTTTCAGGTTAACCAGTAACAACAGTTCACGgtgt            <  2:272121/250‑1 (MQ=255)                                                                                                                                                                                                                                   aacaGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCCGACTGGCGTTTCAGGTTAACCAGTAACAACAGTTCACGTTGTAATGTgcg    >  2:435087/1‑251 (MQ=255)                                                                                                                                                                                                                                    acaGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAACAACAGTTCACGTTGTAATGTgcgc   <  1:339804/251‑1 (MQ=255)                                                                                                                                                                                                                                      agaagaGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCCGGTTAACCCGTAACCACAGTTCACGTTGTAATGTGCGca  >  2:34303/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                        AGTTCAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCAGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAACAACAGTTCACGTTGTAATGTGCGCA  >  NC_000913/670388‑670866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑