| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 458,189 | 0 | A | G | 20.6% | 142.4 / 10.5 | 63 | D255G (GAT→GGT) | clpX | ATP‑dependent Clp protease ATP‑binding subunit ClpX |
| Reads supporting (aligned to +/- strand): ref base A (16/34); new base G (13/0); total (29/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.48e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGACAAAGCAAGC‑GAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGC > NC_000913/457950‑458431 | aGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑GTTGCCGGGCTGGAT‑AAA‑GTGAttt > 1:121650/1‑251 (MQ=255) gCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTc < 2:237851/251‑1 (MQ=255) gCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTTCCGGTCTGGGT‑AAA‑GTGATTTcccccc > 1:63994/1‑248 (MQ=255) tGGTATTGTCTACATCGATGAACTCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGGCGGTCTGGGT‑AAA‑GTGATTTTCCAACgtg > 2:273696/1‑251 (MQ=255) aTTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGAAAGCCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCGGGCGCTGTTGAAACTGAGCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGTCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATTCCTCTAATATCCTGGTTATTTGTGGCGGTGCG‑TTTGCCGGTTTGGAT‑AAA‑GTGATTTCCCACCGTGTAg < 1:332172/250‑1 (MQ=255) tGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGaa < 1:371349/250‑1 (MQ=255) aTCGATGAAATCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTTCCGGTCTGGAT‑AAA‑GTGGTTTCCCCCCGGGTAGAAAACGGCTc > 1:134925/1‑251 (MQ=255) aTGAAATCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGACACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTATAAGATCCTGTTTATTTGTGGCGGTGGG‑TTTGCCGGGCTGGATCAAA‑TGGATTTCC‑ACCGTGTAGGAACAGGCTCCGGc > 2:354758/1‑251 (MQ=255) ccGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACGGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGt < 1:153830/250‑1 (MQ=255) cGTAAGGCAGACAACCCGTCCATTACCCGAGACGTTGCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCGAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGGGGAGAAACCGGCTCCGTCATTGGTTTTGGCTCGAc < 2:326486/248‑1 (MQ=255) aaGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGGGCGACGG‑Taa > 1:9415/1‑250 (MQ=255) gTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTTCCGGGCTGGAT‑AAA‑GTGATTTTCCACCGCGGAGAAACCGGCTCCGGCACTGGGTTTTGCGCGACGG‑Taaa > 1:136395/1‑249 (MQ=255) ccGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGa < 1:280783/251‑1 (MQ=255) gTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCGCTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGAGCG‑GTTGCCGGGCTGGGA‑AAA‑GTGATTTTCCACCGTGTAGAAACCGGCTCCGGCATTTGTTTTGGCGCGGCGG‑Taaaaaagaaagacgcc > 1:268972/1‑238 (MQ=255) gTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATGCCTCTAAGATCCTGGTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGACa < 2:360688/251‑1 (MQ=255) cGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTTCCGGGCTGGGT‑AAA‑GGGATTTTCCACCGGGTAGAAACCGGCTCCGGCATTGGTTTTGGGGCGGCGG‑TAAAAGCGAAGACCGGCAaaacaaacg > 2:132989/1‑247 (MQ=255) agaCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAACCTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TGTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGACAaagcaa < 1:90400/245‑1 (MQ=255) agaCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTTCCGGTCTGGGT‑AAA‑GTGATTTTCCACCGTGTAGAAAACGGCGCCCGCCTTGGGTTTGGCGCGGCGG‑GAAAAGCGAAGGCCGGCaaacaaaggcg > 2:21783/1‑241 (MQ=255) agaCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCAGGATGGGT‑AAAAGGGGTTTCCC‑CCGTGGAAAAAACGGATCCGGCGTTGGTTTTGGCGGGGAGG‑TAAAAACGAAGTACGGCaaacgaaaaaagg > 2:90400/1‑241 (MQ=255) ccGTTGCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGACAAAGCAAGC‑GAAGGc < 2:56596/250‑1 (MQ=255) aCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑GTTGTCGGGCTGGGT‑AAA‑GTGGTTTCCCACCGGGTAGAAACCGGCTCCGGCGTTGGTTTTGGGGCGGCGGGTAAAA‑AGGAGTCCCGCAAAGCAAGC‑GAAggg > 1:279263/1‑250 (MQ=255) cGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGACAAAGCAAGC‑GAAGGCg < 1:280467/251‑1 (MQ=255) tttCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑GTTGCCGGTCTGGAT‑AAA‑GGGATTTCCCACCGGGTAGAAACCGGACCCGGCATTGGGTTTTGCGCGACGG‑GCAAAGCGAAGTGCGGCAaagtaagcagaggcg > 1:175627/1‑242 (MQ=255) ttCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGACAAAGCAAGC‑GAAGGCGAg < 2:252750/250‑1 (MQ=255) cGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGACAAAGCAAGC‑GAAGGCGAgctgc < 1:9380/251‑1 (MQ=255) ggTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGACAAAGCAAGC‑GAAGGCGAgctgct < 1:227526/251‑1 (MQ=255) gAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGACAAAGCAAGC‑GAAGGCGAGCTGCTGgc < 1:160994/251‑1 (MQ=255) cagcagGCACTGTTGAAACTGATCGAAGGTACGGTCGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGGCAAAGCAAGC‑GAAGGCGAGCTGCTGGCGCAGGTTGa < 2:125030/251‑1 (MQ=255) agcagGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGACAAAGCAAGC‑GAAGGCGAGCTGCTGGCGCAGGTTGaa < 1:425762/251‑1 (MQ=255) agGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGGTTTGC‑GGGCATGAT‑AAA‑ATGGTTTTCCACCGGGTAGAAAACGGCTCCGGCATTGGTTTTGGCGCGACGG‑TGAAAGCGAAGTCCGACCAACCAAGC‑GAAGGCGAGCTGCCGGCGCAGGGTGAAcc > 2:273737/1‑250 (MQ=255) agGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGACAAAGCAAGC‑GAAGGCGAGCTGCTGGCGCAGGTTGAACCg < 2:139748/251‑1 (MQ=255) aCTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGACAAAGCAAGC‑GAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAg < 1:177926/251‑1 (MQ=255) ttgcaaCTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGGTTTGC‑GGTCTGGGT‑AAC‑ATGATTTCCCCCCGGGGAGAAAACGGCTCCGCCATTGGGTTTGTTGCGACCG‑GAAAAACGAAGCACGAAACAGCAACG‑GAACGCGCGCTGCTGGCGCCGGttacacaggaagagct > 2:152904/5‑237 (MQ=255) gAAAATGAACGAAGGTAAGGTCGCGGCTGTTCAACCGAAAGGTGGGCGTACATATCCGCAGCGGGAATTCTTGCAGGTTGCTAGCTCTGCGAGCCGGTTTATTTGTGGCGGTGTG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAAGCGGCTCCGGCATTGGTCGTGGCGCGACGG‑TAAAAGCGAAGTGCGACAAAGCAAGC‑GAAGGCGAGCTGCTGGCGCAGGTTGACCCGGAAGATCtta < 2:180002/251‑3 (MQ=255) gATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGACAAAGCAAGC‑GAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCaa < 2:207120/249‑1 (MQ=255) cGAAGGTACGTTAGCTGCTGTTCCACAGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGAGCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGACAAAGCAAGC‑GAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTg < 2:373046/251‑1 (MQ=255) gTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGACAAAGCAAGC‑GAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCtt < 1:132989/251‑1 (MQ=255) aCGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGTCGGTCTGGGT‑AAA‑GTGATTTCCCCCCGTGGTGAAAACGGCACCCGCATTGGTTTTGGCGCGGCGG‑TTAAAGCGAAATCACACCAAGAAAGCCGAAG‑CGGGCTGCTGGCGCAGGGTGAACCGGACGAACTGCTTAAATTTGGTCTTAt > 2:386758/1‑251 (MQ=255) gctgctGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑GAAAAGCGAAGTCCGACAAAGCAAGC‑GAAGGCGAGCTGCTGGCGCAGGTTGAAACGGAAGATCTGATCAAGGTTGGTCTTATCCCTg > 1:284725/1‑250 (MQ=255) gctgTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGACAAAGCAAGC‑GAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGt < 2:329522/250‑1 (MQ=255) gctgTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGACAAAGCAAGC‑GAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGt < 2:279263/250‑1 (MQ=255) aCCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGGT‑AAA‑GGGATTTCCCACCGGGGAGAAAACGGCTCCGGCATTGGGTTTGGCGGGACGG‑GAAAAGCGAAGGCCGGCAAAGCAAGC‑GAAGGGGAGCTGCTGGCGCCGGGTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGgtc > 1:23070/1‑251 (MQ=255) gggctgaattctcagcagcaGGAATGCTTGCAGGTTGATACCTCTAAGATCCTGTTTATGTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGACAAAGCAAGC‑GAAGGCGAGCGGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTGATTGGTCGCCTGCCgg < 2:366167/236‑1 (MQ=255) gggCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCCCCGTGTAGAAACCGGCTCCGGCATTGGGTTTTGCGCGACGG‑TAAAAGCGAAGTCCGACAAAGCAAGC‑GAAGGCGAGCTGCTGGCGCCGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCggg > 1:27347/1‑250 (MQ=255) ggCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGGG‑GTTGCCGGTCTGGGT‑AAA‑GTGATTTTCCACCGTGTAGAAAACGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGACAAAGCAAGC‑GAAGGCGAGCGGCTGGCGCAGGTTGAAACGGAAGATGTGGTCAAGGTTGGTCTTATCCCCGAGGTTATTGGTCGTGTGCCGGtt > 1:60987/1‑251 (MQ=255) cATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGGT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTTGCGCGACGG‑TAAAAGCGAAGTCCGACAAAGCAAGC‑GAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGGCTTATCCCTGAGTTTTTTGGTCGGCTGCCGGGTGTCGCAAc > 1:49279/1‑251 (MQ=255) cATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑GTTGCCCGTCTGGAT‑AAA‑GTGATTTTCCACCGTGTAGAAACCGGCCCCGGCATTGGTTTTGGCGCGCCGG‑GAAAAGCGGAGTCCGACAAAAGAAGC‑GAAGGCGAGCTGCTGGCGCCGGGTGAAACGGAAGATCTGATCAAGTTTGGTCTTTTCCCTGAGGTTTTTGGGCGGCTGCCGGGTGTCGCAAc > 1:184897/1‑251 (MQ=255) ccGCAGCAGGAATTCTTGCAGGTTGATACCGCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGACAAAGCAAGC‑GAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGtt < 2:87392/251‑1 (MQ=255) cGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGACAAAGCAAGC‑GAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTAGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTtg < 1:259463/251‑1 (MQ=255) gcagcaGGAATTCTTGCAGGTTGATTCCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGACAAAGCAAGC‑GAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTtga < 1:105212/251‑1 (MQ=255) gcagGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCTGGGCTGGAA‑AAA‑GTGATTTCCCACCGTGTAGAAAACCGCACCCGCATTGGTTTTGGCGCGACGG‑GAAAAACGAAGTCCGACAAAGCAAGC‑GAAGGCGAGCTGCTGGCGAAGGGTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTAATGGTCGTCTGCCGGTTGTCGCAACGTtgaagg > 2:239118/1‑249 (MQ=255) gcagGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCgg < 2:265534/105‑1 (MQ=255) aTTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTATGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCCGCCCCGGCATTGGTTTTGGCGCGGCGG‑TAAAAGCGAAGTCCGACAAAACAAGC‑GAAGGCGAGCTGCTGGCGCAGGTTGGACCGGAAGATCTGGTCAAGTTTGGTCTTATCCCTGAGGTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTg > 1:398919/1‑250 (MQ=255) cAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGACAAAGCAAGC‑GAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGGTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCgaaga < 2:127672/251‑1 (MQ=255) ggTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGGCCGGGT‑GAA‑GGGATTTTCCACCCGGTAGAAACCGGCACCGGCATTGGTTTTGGCGCGGCGG‑GAAAAACGAAGTCCCGCAAAGCAAAC‑GAAGGCGAGCGGCTGGGGGAGGGTGAACCGGGAAAACTGGACAAGTTTGGTCTTATCCCTGAGGTTTTTGGTCGGCTGCCGGGTGTGGCAACGATGGATGAACTGAGCgacgaa > 1:6876/1‑250 (MQ=255) gTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑GTTGCCCGTCTGGAT‑AAA‑AGGATTTCCCACCGGGTAGAAACCGGCCCCGGCATTGGTTTTGGCGCGACGG‑TGAAAGCGAAGGCCGACAAAGCAAGC‑GAAGGGGAGCCGCTGGCGCCGGTTGAACCGGCAGAGCTGATCAAGTTTGGTCTTCTCCCCGGGGTTATTTGTCGTCTGCCGGTTGTCGCCACGTTGGATGAACTGGGCgcagcagc > 2:70175/1‑244 (MQ=255) taattggggCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGCTTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGACAAAGCAAGC‑GAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCTTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAagag < 2:268972/243‑1 (MQ=255) aTTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGACAAAGCAAGC‑GAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGc < 2:252874/250‑1 (MQ=255) tgtgGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGACAaagcaagc < 1:121531/108‑1 (MQ=255) gtgGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGACAAAGCAAGC‑GAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAATAGCCGaaa < 1:406081/251‑1 (MQ=255) ggTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGACAAAGCAAGC‑GAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTAAGATCCTCAAAGAGCCGAAAAACGc > 2:165618/1‑251 (MQ=255) gTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGACAAAGCAAGC‑GAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGcc > 2:286866/1‑251 (MQ=255) gTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGACAAAGCAAGC‑GAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGc > 2:169723/1‑249 (MQ=255) | AGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCG‑TTTGCCGGTCTGGAT‑AAA‑GTGATTTCCCACCGTGTAGAAACCGGCTCCGGCATTGGTTTTGGCGCGACGG‑TAAAAGCGAAGTCCGACAAAGCAAGC‑GAAGGCGAGCTGCTGGCGCAGGTTGAACCGGAAGATCTGATCAAGTTTGGTCTTATCCCTGAGTTTATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGC > NC_000913/457950‑458431 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |