Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,326,450	0	T	G	35.0%	61.6 / 10.7	40	G114G (GGT→GGG)	atoB	acetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (4/22);  new base G (14/0);  total (18/22)
Fisher's exact test for biased strand distribution p-value = 1.32e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGTAATTAAAGCCGCCATTGAACGTGCAAAAATCGATTCACAACACGTTGATGAAGTGATTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGTATGG‑AAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACA‑GGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCA‑TGGTTATCATAT‑GGGGATTACCGCCGAAAACGTGGCTAAAGAGTACGGA‑ATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTCACAGCGTAAAGCGGCAGCCGCAATTGAGTCCGGTGCTTTTAC  >  NC_000913/2326204‑2326698                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                               aGTAATTAAAGCCGCCATTGAACGTGCAAAAATCGATTCACAACACGTTGATGAAGTGATTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGTATgg                                                                                                                                                                                                                                                            <  1:174619/251‑1 (MQ=255) aGTAATTAAAGCCGCCATTGAACGTGCAAAAATCGATTCACAACACGTTGATGAAGTGATTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGGATgg                                                                                                                                                                                                                                                            >  1:410639/1‑251 (MQ=255)     agtAAAGCCGCCATTGAACGTGCAACACTAGATTCACAACACGTTGATGAAGTGATTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGCAACGGTGTGCGGATTCACGGTCAATCAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑CGGGGATgggac                                                                                                                                                                                                                                                         >  2:113553/3‑247 (MQ=255)                 aTTGAACGTGCAAAAATCGATTCACAACACGTTGATGAAGTGATTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGGATGG‑gaaaaattaggttatc                                                                                                                                                                                                                                           >  2:275228/1‑239 (MQ=255)                     aaCGTGCAAAAATCGTTTCACAAAACGTTGTTGAAGTGATTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGCCGCGTCCGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGAATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCGTTGTGGCGGCGGGGttggtagaaatgaagtttggcccc                                                                                                                                                                                                                                         >  2:157861/1‑228 (MQ=255)                      aCGTGCAAAAATCGATTCACAACTCGTTGATGAAGTGATTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGTATGG‑AAAATAT‑GAGTTTAGCCCCCt                                                                                                                                                                                                                                     <  1:378877/251‑1 (MQ=255)                            aaaaaTCGATTCACAACACGTTGATGAAGTGATTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGTAGGG‑AAAATAT‑GTGGTTAGccccccaattat                                                                                                                                                                                                                               >  1:342571/1‑244 (MQ=255)                              aaaTCGATTCACAACACGTTGATGAAGTGATTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGTATGG‑AAAATAT‑GAGTTTAGCCCCCTACTTACTc                                                                                                                                                                                                                             <  2:408888/251‑1 (MQ=255)                                   gATTCACAACACGTTGATGAAGTGATTATGGGTACCGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCGTGCCGCCCAGGCCATTCAGGCAGGTCAGGCTCAGAGCATTGTGGCGG‑GGGGTATGG‑AAAATAT‑GAGTTTGGCCCCCTAGTTACACGATGc                                                                                                                                                                                                                        <  2:157022/251‑1 (MQ=255)                                            ggcGTTGATGAAGTGATTATGGGTAACGGGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGGTGGCAGAAACGGTGTGCGGATGCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGTATGG‑AAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACg                                                                                                                                                                                                               <  2:134700/249‑1 (MQ=255)                                                  gatgaAGTGATTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGTATGG‑AAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTg                                                                                                                                                                                                          <  1:275228/250‑1 (MQ=255)                                                  gaggaAGTGATTAGGGGTAACGGGTTACAAGCCGGGCTGGGGCAAAATCCTGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTGAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGTATGG‑AAAATAT‑GAGATTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTgg                                                                                                                                                                                                         <  2:376744/248‑1 (MQ=255)                                                     gaAGTGATTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGTATGG‑AAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTa                                                                                                                                                                                                      <  1:130874/251‑1 (MQ=255)                                                     gaAGTGATTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGACACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCAAGGTCAATAAAGTATGTGGATCGGGTCTTAAAAGTGTGGCGCTTGCCGCACAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGGATGG‑AGAAAAT‑AAGATTATCCCCCTCCTTACTCGaagtaaaaagaacgtccggatt                                                                                                                                                                                                      >  1:369185/1‑230 (MQ=255)                                                        gTGATTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGTATGG‑AAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCg                                                                                                                                                                                                   <  1:408895/251‑1 (MQ=255)                                                          gATTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGTATGG‑AAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTc                                                                                                                                                                                                 <  1:207764/251‑1 (MQ=255)                                                           aTTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGTATGG‑AAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCt                                                                                                                                                                                                <  2:253808/251‑1 (MQ=255)                                                             tATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGTATGG‑AAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCtt                                                                                                                                                                                               <  1:98024/250‑1 (MQ=255)                                                                  gTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGTATGG‑AAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGAc                                                                                                                                                                                         <  2:266646/251‑1 (MQ=255)                                                                   aaaCGTTTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTGAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGTATGG‑AAAATAT‑GATTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACg                                                                                                                                                                                        <  2:839/250‑1 (MQ=255)                                                                          ttACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGGATGG‑AAAATAT‑GAGTTTAGCCCCCTACTTACTCGCTGCAAAAGCACGCTCTGGGTATCGTCTTGGAGACGGGCA‑ggg                                                                                                                                                                                >  1:319348/1‑250 (MQ=255)                                                                                    ggCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGGATGG‑GAAAAAT‑GAGGTTAGCCCCCTACTTACTCGATGCAAAAGCACGCGCTGGGTATCGTCTAGGAGACGGACA‑GGGTTATGACGt                                                                                                                                                                       >  1:192265/1‑250 (MQ=255)                                                                                                             ggCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGGATGG‑GAAAAAT‑GAGGTTAGCCCCCCACTTACTCGATTCAAAAGCACCCCCAGGTTATTGTATTGGATAAGGACA‑GGTTTATGACGGAATCCttcccgctgggccggagtgcg                                                                                                                                             >  2:333324/1‑231 (MQ=255)                                                                                                                 cTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGGATGG‑AAAATAT‑GGGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGGGACGGGCA‑GGGTTATGACGTAATCCTGCGCGATGGGCCGATGTGCGCCAc                                                                                                                                         >  1:28002/1‑251 (MQ=255)                                                                                                                                cTGGCAGAAACGGGGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGGATGG‑AAAATAT‑GGGGTTAGGCCCCTACTTACTCGATGCAAAAGCAAGCCCTGGTTTTCGTCTTGGAGAAGGGCA‑AGGTTATGACTTAAACATGCGCGAAGACCTGATGGGGGCCACCCC‑Tgggtttccttt                                                                                                                         >  1:91424/1‑242 (MQ=255)                                                                                                                                  ggCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGGGTGG‑AAAATAT‑GAGGTTAGCCCCCCACTTACTCGATGCAAAAAAACGCTCTGGGTAACGTCTTGGAGACGGAAA‑GGGTTATGACGTAAAACTGCGCGATGGGCTGATGTGCGCCACCCA‑AGGGTATCATAT‑gg                                                                                                                      >  1:253548/1‑251 (MQ=255)                                                                                                                                                       cACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGGATGG‑GAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCAAGCTCTGGGTATCGTCTTGGAGACGGACA‑GGGTTATGACGGAATCCTGCGCGATGGCCCGATGTGAGCCACCCA‑TGGTTATCATAT‑GGGGGTTACCGCCGAAAACGTgg                                                                                                 >  1:233745/1‑251 (MQ=255)                                                                                                                                                       cACGGTCAATAAAGTATGTGGTGCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGTATGG‑AAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACA‑GGTTTATGACGTAATCCTGCGCGATGGCCGTATGGGCGCCACCCA‑TGGTTATCATAT‑GGGGATTACCGCCGAAAACGTg                                                                                                  <  2:410639/250‑1 (MQ=255)                                                                                                                                                        aCGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGTATGG‑AAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACA‑GGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCA‑TGGTTATCATAT‑GGGGATTAccgccg                                                                                                          <  1:279514/241‑1 (MQ=255)                                                                                                                                                        aCGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGTATGG‑AAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACA‑GGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCA‑TGGTTATCATAT‑GGGGATTACCGCCGAAAACGTGGc                                                                                                <  1:419866/251‑1 (MQ=255)                                                                                                                                                                   aggaggtgGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCGGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGTATGG‑AAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACA‑GTTTTATGACGTAATCCTGCGCTATGGCCTGATGTGCGCCACCCA‑TGGTTATCATAT‑GGGGATTCCCGCCGAAAACGTGGCTAAAGAGTACg                                                                                     <  2:163117/246‑1 (MQ=255)                                                                                                                                                                                           ggtgGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGTATGG‑AAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACA‑GGTTTATGACGTAAGCCTGCGCGATGGCCTGATGTGCGCCACCCA‑TGGTTATCATAT‑GGGGATTACCGCCGAAAACGTGGCTAAAGAGTACGGA‑ATTACCCGTGAAAGGCAGGATg                                                            <  1:260852/250‑1 (MQ=255)                                                                                                                                                                                            gtagcgcTTGCCTCCCAGTCCATCCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGTATGG‑AAAATAT‑GAGTTTAGCCCCCTACTTCCTCTATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGCCGGACA‑GGTTTATCACGTAATCCTGCGCGATGCCCTGATGTGCGCCCCCCA‑TGGTTATCATAT‑GGGGATTACCGCCGCAAACGTGGCTAAAGAGTACGGA‑GTTCCCCGTTAAATGCAGGATGa                                                           <  2:356422/248‑1 (MQ=255)                                                                                                                                                                                                              gCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGGATGG‑GAAATTT‑GAGATTCGCCCCCTCCTTACTCGATGCAAAAGCACCCTCTGGTTATTGTCTTGGGGACGGACATGGTTT‑TTACGGAAACCTGCGCTATGGCATGGTGTGCGCCACCCC‑TGGTTATCCTATTGGGGGTTAC‑GCCGGAAACGGGGCGCAAGAATACGGACATTCCCCGGGAAA‑GCAgggggcaatggcgctcacctcacc                                         >  2:130533/1‑229 (MQ=255)                                                                                                                                                                                                                           gTCAGGCGCAGAGCATTGTGGCGG‑GGGGTATGG‑AAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACA‑GGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCA‑TGGTTATCATAT‑GGGGATTACCGCCGAAAACGTGGCTAAAGAGTACGGA‑ATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTCACAGCGTAAAGCGGCa                            <  1:397005/251‑1 (MQ=255)                                                                                                                                                                                                                            tCAGGCGCAGAGCATTGTGGCGG‑GGGGGATGGGAAAATTTTGA‑TTT‑GCCCCCCACTTACTCGATGCAAAAACACGCCCTTGTTTTCGTCTTGGGGAAGGACA‑GGGTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCACTGGT‑ATCATAT‑GGGGGTTACCGCCGCAAACGTGGCTAAAGAGTACGGC‑ATTACCCCTGAAATGCAGGATTAAATGGCGCTAAATTCACAGCGTAAAGCGGccg                           >  2:114056/1‑249 (MQ=255)                                                                                                                                                                                                                              aGGCGCAGAGCATTGTGGCGG‑GGGGTATGG‑AAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCCCTGGGTATCCACTTTGAGGCGGGCC‑GGTTTATTACGTAATCCTGCGCGATGGCCTCATGTGCGCCACCCA‑CGGTTATCATAT‑TGGGGTTACCGCCGAAAACGTGGCTTAAGGGTACGGA‑ATTAACCCTGAAATGCAGGATGGACTGGCGCTCCAATTACCACGTAAAGCGGCAGcc                         >  1:188277/1‑251 (MQ=255)                                                                                                                                                                                                                                      agCATTGTGGCGG‑GGGGGATGG‑GAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCCCTGGGTATCGTCTTGGAGACGGACA‑GGGTTATGACGGAATCCTGCGCGATGGCCTGATGGGCGCCACCCA‑TGGTTATCATAT‑GGGGATTACCGCCGAAAACGCGGCTAAAGAGGACGGA‑ATTAACCGGGGAAAGCAGGATGAAATGGCGCTACATTCACAACGGAAAGCGGCAGCCGCAATTg                  >  1:94565/1‑250 (MQ=255)                                                                                                                                                                                                                                                ggg‑ggggTATGG‑AAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACA‑GGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCA‑TGGTTATCATAT‑GGGGATTACCGCCGAAAACGTGGCTAAAGAGTACGGA‑ATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTCACAGCGTAAAGCGGCAGCCGCAATTGAGTCCGGTGCt       <  2:319348/250‑1 (MQ=255)                                                                                                                                                                                                                                                cGG‑GGGGTATGG‑AAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACA‑GGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCA‑TGGTTATCATAT‑GGGGATTACCGCCGAAAACGTGGCTAAAGAGTACGGA‑ATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTCACAGCGTAAAGCGGCAGCCGCAATTGAGTCCGGGGCt       >  2:410331/1‑251 (MQ=255)                                                                                                                                                                                                                                                       gTATGG‑AAAATAT‑GAGTTTAGCCCCCTACTTACTAGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGATGGACA‑GGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCA‑TGGGTATCATAT‑GGGGATTACCGCCGAAAACGTGGCTAAAGAGTACGGA‑ATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTCACAGCGTAAAGCGGCAGCCGCAATTGAGTCCGGTGCTTTTAc  >  1:163663/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                               AGTAATTAAAGCCGCCATTGAACGTGCAAAAATCGATTCACAACACGTTGATGAAGTGATTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGG‑GGGGTATGG‑AAAATAT‑GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACA‑GGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCA‑TGGTTATCATAT‑GGGGATTACCGCCGAAAACGTGGCTAAAGAGTACGGA‑ATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTCACAGCGTAAAGCGGCAGCCGCAATTGAGTCCGGTGCTTTTAC  >  NC_000913/2326204‑2326698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑