Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,640,045	+C	coding (13/534 nt)	rrrQ ←	putative lysozyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,640,045	1	.	C	97.1%	124.1 / ‑3.7	34	coding (13/534 nt)	rrrQ	putative lysozyme
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (12/21);  total (12/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

CCTTATCACGCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGAT‑TTTTGTGTTCATAGATATTTATGATGAGGACGCTCGTGCTTATTGGCAGGATTTTCAATCTTAAAGGAGTACTGATGCTGCAGATAAGACTCAACTTTTTCTGACAATTTTTCTGCTACTTCCAGGAAGACTTGCCGGACGCTCCTTCTGGCTGCTGCCTCATAAAACTCCAGCGCAGCTCCTTCAACACGGTCCATGGCGACATCCAGGTCAAAAATTTCACCGTCAAAGCGTTCTTTG  >  NC_000913/1639805‑1640285                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 ccTTATCACGTTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGtt                                                                                                                                                                                                                                         <  2:9741/251‑1 (MQ=255) ccTTATCACGCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTAGGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGAT‑CTTtggtgtt                                                                                                                                                                                                                                        <  2:159331/251‑6 (MQ=255)    tATCACGCTCAATGGCGTTTACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAc                                                                                                                                                                                                                                      >  1:229948‑M1/1‑250 (MQ=255)                       gACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacg                                                                                                                                                                                                                   >  1:329260‑M1/1‑231 (MQ=255)                                           tCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttg                                                                                                                                                                                                <  1:375771‑M1/250‑40 (MQ=255)                                                        atTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactga                                                                                                                                                                                    >  2:387724‑M1/1‑198 (MQ=255)                                                          tGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagct                                                                                                                                                                                >  1:161138‑M1/1‑196 (MQ=255)                                                          tGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagct                                                                                                                                                                                >  1:313839‑M1/1‑196 (MQ=255)                                                           gggAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCGcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagct                                                                                                                                                                                <  1:107792‑M1/250‑56 (MQ=255)                                                               aaaaCGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccg                                                                                                                                                                           >  1:389147‑M1/1‑191 (MQ=255)                                                                        tttCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgc                                                                                                                                                                   <  2:161138‑M1/250‑69 (MQ=255)                                                                         ttCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcc                                                                                                                                                                  <  1:127163‑M1/250‑70 (MQ=255)                                                                                                  aCAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttc                                                                                                                                        >  1:160091‑M1/1‑156 (MQ=255)                                                                                                         gTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAACCTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCACCCGACAGGCCGTATCTGTTCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaact                                                                                                                                 <  2:247378‑M1/251‑103 (MQ=255)                                                                                                                 gCCAGACCCATCGCGGTATGCCCTTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGACCGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttctggaactaaatccc                                                                                                                          <  2:238432‑M1/250‑110 (MQ=255)                                                                                                                  ccAGAACCATCGCGGTATGCCATTTTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaacgaaatcccct                                                                                                                        <  2:346003‑M1/251‑112 (MQ=255)                                                                                                                    aGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactga                                                                                                                                                                                    <  1:397876‑M1/189‑52 (MQ=255)                                                                                                                    aGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactga                                                                                                                                                                                    >  2:397876‑M1/1‑138 (MQ=255)                                                                                                                     gAACCATCGCGGTATGCCATTGTGTGGTTACCTACTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttc                                                                                                                     <  2:24466‑M1/251‑115 (MQ=255)                                                                                                                                    gCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcga                                                                                                       <  1:198959‑M1/250‑129 (MQ=255)                                                                                                                                      cATTGTGTGGTTACCTTCTTTTACGACCAGAAACTGGTGAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagc                                                                                                    <  1:206553‑M1/251‑132 (MQ=255)                                                                                                                                         tgtgtgGTTACCTTCTTTTTCGTCCAGAACCTGGTCAAGTATCTGGGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagcca                                                                                                  <  2:60357‑M1/250‑134 (MQ=255)                                                                                                                                         tgggtggttACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGAGGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagcca                                                                                                  <  2:314210‑M1/247‑134 (MQ=255)                                                                                                                                                  ccctTCTTTTCCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacc                                                                                        <  2:314913‑M1/250‑144 (MQ=255)                                                                                                                                                           gtcGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAAGCATCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttg                                                                               <  2:12752‑M1/250‑153 (MQ=255)                                                                                                                                                            tCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttg                                                                               <  1:200344‑M1/250‑153 (MQ=255)                                                                                                                                                             cGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgt                                                                              <  2:224160‑M1/250‑154 (MQ=255)                                                                                                                                                                  aGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagt                                                                        >  1:51824‑M1/1‑92 (MQ=255)                                                                                                                                                                                                               ggcgccAGACCGGCAGCCGACAGGCCGTAACTGGTCTTTAGTGTTCAcccgcccgcttcgtcttacgatattcatcataaacttggggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatacaaaagagcgtcagttttaagcatt                           <  2:304709‑M1/250‑205 (MQ=255)                                                                                                                                                                                                                       aaCGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatacaaaagagcgtcagttttaagcatttaaaacac                   <  1:387724‑M1/251‑213 (MQ=255)                                                                                                                                                                                                                        aCGGCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatcttcatcatacactttgggttcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccctcgggcactaaatcccctttccagctataaagcgaagccaaacgactacctgctgcctgcgcaagttttgtttttgaaccgaaatacaaaagagcgtcagttttcagcatttaaaacacc                  >  2:57623‑M1/1‑38 (MQ=255)                                                                                                                                                                                                                          ggCAGCCGACAGGCCGTATCTGATCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatacaaaagagcgtcagttttaagcatttaaaacacctt                >  1:289364‑M1/1‑36 (MQ=255)                                                                                                                                                                                                                                  aCAGGCCGTATCTGATCTTTTGGGTTCAaccgcccgcttcgtcttacgatattcatcataaacgttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagcgataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaactgaaatacaaaagagcgtcagttttaagcatttaaaacacctttattgtta        <  2:74056‑M1/251‑224 (MQ=255)                                                                                                                                                                                                                                         gcatcTGATCTTTTGTGTTCAcccgcccgattcgtcttacgatattcatcataaactttgggatcacaccgaagctccccgccagatgcctcctgtaggcgcatcgcgcgaccttcgggaactaaatcccatttccagctataaagcgaagccaaacgaatacctgatgcttgtgcacgttttgtttttgacccgaaatccaaatgaacgtcagttttaagcatttaaaacaacttaattgttagtcatt  >  2:394187‑M1/3‑21 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 CCTTATCACGCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGAT‑TTTTGTGTTCATAGATATTTATGATGAGGACGCTCGTGCTTATTGGCAGGATTTTCAATCTTAAAGGAGTACTGATGCTGCAGATAAGACTCAACTTTTTCTGACAATTTTTCTGCTACTTCCAGGAAGACTTGCCGGACGCTCCTTCTGGCTGCTGCCTCATAAAACTCCAGCGCAGCTCCTTCAACACGGTCCATGGCGACATCCAGGTCAAAAATTTCACCGTCAAAGCGTTCTTTG  >  NC_000913/1639805‑1640285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑