| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,312,070 | Δ1 bp | intergenic (‑28/+31) | alsB ← / ← alsR | D‑allose ABC transporter periplasmic binding protein/DNA‑binding transcriptional repressor AlsR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,312,069 | 0 | G | . | 94.1% | 144.8 / 1.9 | 34 | intergenic (‑27/+32) | alsB/alsR | D‑allose ABC transporter periplasmic binding protein/DNA‑binding transcriptional repressor AlsR |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base . (15/17); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGCAATTGAGATTGAAAATCGCCTTCTGAAGGAGAGGCAAAAATATCAACGCTGACGCCCAGTGTTTTTGCTTCATCTTCAATGCCTTTTTTCATATCTACCCAAAATGGGTTGGAGAGGGTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCG > NC_000913/4311824‑4312309 | gattttgccgttatcgacgaccaggttgattttgcaacctgatgcgcaataggggcaaaccgtgacgacttttttcatcggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACt > 1:53019‑M2/234‑251 (MQ=255) ttatcgacgaccaggttgattttgcaacctgatgcgcaataggggcaaaccgtgacgacttttttcatcggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGc > 1:99565‑M2/224‑251 (MQ=255) gaccaggttgattttgcaacctgatgcgcaataggggcaaaccgtgacgacttttttcatcggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTAc < 1:294865‑M2/36‑1 (MQ=255) caacctgatgcgcaataggggcaaaccgtgacgacttttttcatcggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACGCCGCGATGGCGGAATTACCTTTTTCTTATTTCAg < 2:127398‑M2/52‑1 (MQ=255) gcgcaataggggcaaaccgtgacgacttttttcatcggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTg < 1:111513‑M2/60‑1 (MQ=255) gcgcaataggggcaaaccgtgacgacttttttcatcggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTg < 1:1723‑M2/60‑1 (MQ=255) tttttcatcggtctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCg < 2:52569‑M2/86‑1 (MQ=255) tttttcatcggtctcgctccagttaatcaaatcacgcatacgcgctcgcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaacgctgacgaaaaacaggctgtcggctgttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTGCAGCGCGCCTGTTCAGAATAAATCAACAAGTGCGCCg < 2:100230‑M2/86‑1 (MQ=255) catcggtctggctccaggtaatcaaatcacgcaaacgcgctctcgactacagtatgcatctattatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGTGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGAttttt < 2:103515‑M2/92‑1 (MQ=255) cagcggtctcgctacagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgttgggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGttttt < 2:87473‑M2/92‑1 (MQ=255) ctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaatcaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCcggttttttcca < 2:197250‑M2/96‑4 (MQ=255) ctcgctccagttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaatcaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCcggttttttcca > 1:197250‑M2/152‑244 (MQ=255) gttaatcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatcttacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCACCAATTGCGCCGGTTTTTTCCATCTTAATATTAGc > 2:303194‑M2/143‑251 (MQ=255) atcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGt < 1:269824‑M2/113‑1 (MQ=255) aaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCa < 1:141692‑M2/116‑1 (MQ=255) gcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactattcattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTg > 1:102957‑M2/129‑250 (MQ=255) acgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTg > 1:220831‑M2/125‑251 (MQ=255) ctacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGAc > 2:149839‑M2/113‑251 (MQ=255) cacagttgatgtggggtcattcccggatattacgggcactatttagtcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGATA‑GTAACTCCGCGATGGCGGAAGTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTGTTTTCCATATTAATATTAGCTTTTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAggtt < 2:151644‑M2/161‑1 (MQ=255) ttatgtggggtcattacctgatattacgggccctatttattcaaaactctgacgaaaaacaggctgtcgtacgttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGCATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTACTATTAGCTTGTTCACTGTTGAGCTGGCCGACAGAGACAAAAAAcgcgtcaagcaaggctggttgt > 2:75655‑M2/85‑241 (MQ=255) gggtcattacctgatatgacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTc < 2:83060‑M2/174‑1 (MQ=255) cattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTg < 1:262554‑M2/177‑1 (MQ=255) atattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCAtt < 1:149839‑M2/187‑1 (MQ=255) ggcactatttattcaacactctgacggaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGTTGGCGGGATTCCCTTTTTCTTATTTCAACGCGCCTGGTGAAAAGAAATCAACAATTGCGCCGGCTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGTCGACAGAGACAAAATAAGCGTACAGCCAGGTTGGGTGTAATATTCTTGCCGAGGCATTACGACCt > 2:238158‑M2/57‑250 (MQ=255) aaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTg > 1:273393‑M2/31‑249 (MQ=255) aacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGTTGAGCa > 2:395741‑M2/28‑250 (MQ=255) acaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCaa > 2:308749‑M2/27‑250 (MQ=255) caggctgtcgtcagttttgacgtgaGGTAATGGTACA‑GTAACTCCGAGATGGCGGAATTACCTTTTTCTTATGTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTGCCAGATTAATATTAGCGGGTTCAATGTTGAGCTGGGCGACAGTGACAAAAAAAGCGGCCAGCAAGGTTGGTTGTAAGATGCTTGCCGAGGCATTACGACTTAATAACGGCGTTTCCGGGGCTGTTGAGCAaat < 2:134858‑M2/226‑1 (MQ=255) aggctgtcgtcggttttgacgtgaGGTAAGGGGCCAGG‑AACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCACGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAaat > 2:246136‑M2/25‑250 (MQ=255) aggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGCCCTAATAACGGCGTTTCCGGGGCTGGTGAGCAaat > 1:430425‑M2/25‑250 (MQ=255) gtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATc > 1:102236‑M2/17‑251 (MQ=255) tttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCa < 1:308749‑M2/240‑1 (MQ=255) gtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGc > 2:291562‑M2/5‑251 (MQ=255) ggaaGGGGACAG‑TAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCGTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCg < 1:368127/249‑1 (MQ=255) | CTGCAATTGAGATTGAAAATCGCCTTCTGAAGGAGAGGCAAAAATATCAACGCTGACGCCCAGTGTTTTTGCTTCATCTTCAATGCCTTTTTTCATATCTACCCAAAATGGGTTGGAGAGGGTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCG > NC_000913/4311824‑4312309 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |