Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	535,337	T→C	A474A (GCT→GCC)	gcl →	glyoxylate carboligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	535,337	0	T	C	100.0%	198.9 / NA	55	A474A (GCT→GCC)	gcl	glyoxylate carboligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/28);  total (27/28)

GTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGAAAGCCTTAATGGCGCAATATCGGGTACCGGTAGTCGTGGAAGTTATTCTCG  >  NC_000913/535096‑535581                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     gTCTGTCACACATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAACTGTGGCCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGCTTGAAGCGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCCACACCGCCAATCTgggg                                                                                                                                                                                                                                             >  2:210303/1‑251 (MQ=255)      gcacaAATCGCTGCGAAACAATGGCTGAATGTGTTTAAGGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTGAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCGGATCCGAAACGCAAGGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTGGGCGCGCAGTTCAACTTTCCGGACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGAt                                                                                                                                                                                                                                        <  1:60123/250‑1 (MQ=255)               gcggCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCgtcagtc                                                                                                                                                                                                                               <  1:450341/248‑1 (MQ=255)               gCTGCGGCACAAATGCTGGATGTCTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCgtcagtc                                                                                                                                                                                                                               >  2:191986/1‑251 (MQ=255)                cTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAACTGTGGTAAGGCTGGTCCGTTCGGCTGGACGATTCCGGCTGCGCTCGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGTCTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGAAGTTCAACATTCTGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCgtcagtc                                                                                                                                                                                                                               >  2:462330/1‑250 (MQ=255)                   gggcaaaagtgcgtgttttCTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACaa                                                                                                                                                                                                                           <  2:417702/233‑1 (MQ=255)                   cgtcaaaattggggaagggttttAAATACCGCCACGGGATCAGCTGTGGGCAGGCTGGTCCGTTAGGCTGGACTATTCCGGCTGCGTTAGGGGTTTTTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTTGCTACTTTGACTTCCAGTTCCTGATTTAAGAGTGAGCTGGTGGCGCGTAGTTCAACATTCCGTACGCTCATGTGCGGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACaa                                                                                                                                                                                                                           <  1:486152/231‑1 (MQ=255)                      caaaaatgttgattgtattaaaaGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAAcgc                                                                                                                                                                                                                        <  2:240132/231‑1 (MQ=255)                       acaAATGCTGCACGTCTTTAACGACCGCCACCGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGAATACGGATGCGCTAGGGGTTTGTGCCGCTGATACGACACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTACAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAAcgcg                                                                                                                                                                                                                       >  2:517047/1‑251 (MQ=255)                           aTGCTGCATGTCTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCCCAACGCGCttt                                                                                                                                                                                                                   >  2:168034/1‑251 (MQ=255)                             gctgcATGTCTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTg                                                                                                                                                                                                                 >  1:272327/1‑251 (MQ=255)                                           aaGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGc                                                                                                                                                                                                   >  1:360418/1‑251 (MQ=255)                                            aGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGCTTCCGGCTGCGCCAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCg                                                                                                                                                                                                  >  1:209575/1‑251 (MQ=255)                                             gACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGt                                                                                                                                                                                                 >  1:503845/1‑251 (MQ=255)                                               ccGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTGGACAGGGACTACTGCGTGc                                                                                                                                                                                               <  1:149900/251‑1 (MQ=255)                                                       ggATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTGGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGc                                                                                                                                                                                        <  1:518959/250‑1 (MQ=255)                                                            aaCTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCgg                                                                                                                                                                                  >  2:10647/1‑250 (MQ=255)                                                                 tgGTCAGGTTGGTCCGTTTGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAat                                                                                                                                                                              >  2:182412/1‑250 (MQ=255)                                                                  ggTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGCTTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAatat                                                                                                                                                                            >  2:36844/1‑251 (MQ=255)                                                                      aGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAAc                                                                                                                                                                        >  1:130503/1‑251 (MQ=255)                                                                           ggTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTcc                                                                                                                                                                     >  1:270646/1‑249 (MQ=255)                                                                              ccGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCagtg                                                                                                                                                                 >  1:300760/1‑250 (MQ=255)                                                                               cGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCagtgaa                                                                                                                                                               <  1:462330/251‑1 (MQ=255)                                                                                   aGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCagtgaagtg                                                                                                                                                            >  2:156064/1‑250 (MQ=255)                                                                                       gggACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATgg                                                                                                                                                       <  2:282264/250‑1 (MQ=255)                                                                                        ggACGATTCCGGCTGCGCTAGGTGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATg                                                                                                                                                        <  2:130503/249‑1 (MQ=255)                                                                                           cggttCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACGTTGACTTCCAGTTCCTTATTTAAGAGTTAGCTGGTGGTGCTCAGTTCATCATTCCTTACATCCAGGTGCTGTGCAAGAATGGCTATCTGGGGCTGGTTCGTCATTGACAACGCGCTTGTGTCATGGACTACGGTGTGGATGTCGGTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTAc                                                                                                                                                   <  1:238216/248‑1 (MQ=255)                                                                                                  ggCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTg                                                                                                                                            >  1:371504/1‑251 (MQ=255)                                                                                                  ggCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTg                                                                                                                                            <  1:210303/251‑1 (MQ=255)                                                                                                         cTAGGGGCTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGt                                                                                                                                     >  1:156027/1‑251 (MQ=255)                                                                                                             gggTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATGCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCGTTCGAGAAAATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTaaaa                                                                                                                                 <  2:238948/251‑1 (MQ=255)                                                                                                                       cGCTGATCCGAAACGCAATGTGGTGGCGATTTCTTGCGACTTTGTCTTCCAGTTCCTGATTGAAGAGTTAGCTTTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAg                                                                                                                       <  1:191986/251‑1 (MQ=255)                                                                                                                         cTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCCTGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGt                                                                                                                     >  2:496464/1‑251 (MQ=255)                                                                                                                          tGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGtt                                                                                                                    >  2:258013/1‑251 (MQ=255)                                                                                                                                gAAACGCAATGTGGTGGCGATTTCTGGCGGCTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTGTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGGGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCTGAAGGTTTAGGtt                                                                                                              <  1:182412/251‑1 (MQ=255)                                                                                                                                gAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGtt                                                                                                              <  2:429305/251‑1 (MQ=255)                                                                                                                                 aaaCGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTg                                                                                                             >  1:507204/1‑251 (MQ=255)                                                                                                                                                         ggCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAAc                                                                                      <  1:238602/250‑1 (MQ=255)                                                                                                                                                             aCTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGaa                                                                                 <  1:10645/251‑1 (MQ=255)                                                                                                                                                                      tCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTGTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTg                                                                          <  1:496464/249‑1 (MQ=255)                                                                                                                                                                        cAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGcc                                                                      >  1:401480/1‑251 (MQ=255)                                                                                                                                                                                gATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTg                                                              <  1:102129/251‑1 (MQ=255)                                                                                                                                                                                                gTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGAAAGCCt                                               <  1:113591/250‑1 (MQ=255)                                                                                                                                                                                                       cgcAGTTCAACATTCCGTACTTCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGCATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCCCGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGCAGATATTGCGGCAGCCTTTGAACAGACGAAAGCCTTAATGgcg                                       >  2:526698/1‑251 (MQ=255)                                                                                                                                                                                                        gcAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGAAAGCCTTAATGgcgc                                      >  2:279819/1‑251 (MQ=255)                                                                                                                                                                                                                aCATTCCGTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGAAAGCCTTAATGGCGCAATATCgg                              <  2:371504/251‑1 (MQ=255)                                                                                                                                                                                                                       gTACATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGATAGGCTTAATGGCGCAATATCGGGTACCgg                       <  1:156064/251‑1 (MQ=255)                                                                                                                                                                                                                         aCATCCATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAGAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGAAAGCCTTAATGGCGCAATATCGGGTACCGGt                      <  1:10647/250‑1 (MQ=255)                                                                                                                                                                                                                              cATGTGCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGACAGCCTTAATGGCGCAATATCGGGTACCGGTAGTCgg                >  1:170620/1‑250 (MQ=255)                                                                                                                                                                                                                                 gtgCTGGTCAACAACGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTGTAGGTTGGAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTGTGAACATGCTAAAGCCTTAATGGCGCAATATCGGGTACCGGTAGTCGTGGa             <  1:517047/251‑1 (MQ=255)                                                                                                                                                                                                                                          aacaacGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGAAAGCCTTAATGGCGCAATATCGGGTACCGGTAGTCGTGGAAGTTATtct    <  2:272327/251‑1 (MQ=255)                                                                                                                                                                                                                                          aacaacGCCTATCTGGGGCTGATGCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGGGTAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGGTGTAAAGCTATGCGGGTCTTCAAACGGGAGGATATTGCGCCAGCCTTGGAACAGGCGGAAGCCGTAATGGCGCAAGATCTTGTGCCGGTAGGCATGGAAGTGATtct    <  2:220399/251‑1 (MQ=255)                                                                                                                                                                                                                                             aacGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCg                                                                                    <  2:238689/168‑1 (MQ=255)                                                                                                                                                                                                                                             aacGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCg                                                                                    >  1:238689/1‑168 (MQ=255)                                                                                                                                                                                                                                             aacGCCTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGAAAGCCTTAATGGCGCAATATCGGGTACCGGTAGTCGTGGAAGTTATTCTCg  >  1:316011/1‑250 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     GTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAACTGTGGTCAGGCTGGTCCGTTAGGCTGGACGATTCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCTATTCGGGTCTTCAAACCGGAAGATATTGCGCCAGCCTTTGAACAGGCGAAAGCCTTAATGGCGCAATATCGGGTACCGGTAGTCGTGGAAGTTATTCTCG  >  NC_000913/535096‑535581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑