Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,558,907	T→A	K207M (AAG→ATG)	iadA ←	isoaspartyl dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,558,907	0	T	A	92.2%	267.9 / ‑4.4	91	K207M (AAG→ATG)	iadA	isoaspartyl dipeptidase
Reads supporting (aligned to +/- strand):  ref base T (0/7);  new base A (50/33);  total (50/41)
Fisher's exact test for biased strand distribution p-value = 2.50e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

CTACCGTTGCCGTCGGAGCTGAGGGTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCTTTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTC  >  NC_000913/4558661‑4559151                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       cacgccgccctgcggatcgtGAGGGTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCGTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCGGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCatttt                                                                                                                                                                                                                                                    <  2:114037/232‑1 (MQ=255)      gTTGCCGTCGGAGCTGAGGGTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑t                                                                                                                                                                                                                                              >  2:447034/1‑251 (MQ=255)              cGGAGCTGAGGGTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCAtg                                                                                                                                                                                                                                      >  1:18054/1‑251 (MQ=255)                 aGCTGAGGGTGACGCGAGCCAGCGGAATAACCGCCTGGACGGAGCGGGCACTACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGAGACGCCTTTGCGCACGAACTCCAGCGCCTGCTCAAACAACGGAACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGATTTCCAGAAGGTAATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCTTGT‑gg                                                                                                                                                                                                                                  >  1:503562/1‑251 (MQ=255)                    tGAGGGTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCGGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCatttt                                                                                                                                                                                                                                                    >  1:114037/1‑232 (MQ=255)                       gggTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACg                                                                                                                                                                                                                            <  1:382594/251‑1 (MQ=255)                         gTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGt                                                                                                                                                                                                                          >  1:385885/1‑251 (MQ=255)                             cgggTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATCCCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACg                                                                                                                                                                                                                      <  2:529029/248‑1 (MQ=255)                             cgcgTGCCAGCGGAATGCCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTGGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCGTGCGGATCGGCACTGCGCAGTTTTCCAGCAGGTCAGAAATAGG‑CTGGAACGCCATTTTACTG‑TCGCTGAAGT‑GGAACTCGGTGACg                                                                                                                                                                                                                      <  2:341922/251‑1 (MQ=255)                              gcgTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGc                                                                                                                                                                                                                     >  1:257290/1‑251 (MQ=255)                                       cGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGCGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTAc                                                                                                                                                                                                            >  2:90153/1‑251 (MQ=255)                                        ggAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTAcc                                                                                                                                                                                                           <  1:38976/251‑1 (MQ=255)                                             ccccGCCTAAACGGCGCGGCCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGa                                                                                                                                                                                                      <  2:257290/250‑1 (MQ=255)                                                 gCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCa                                                                                                                                                                                                   >  1:201974/1‑250 (MQ=255)                                                  ccggAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TAGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCaaa                                                                                                                                                                                                 <  2:112840/248‑1 (MQ=255)                                                       aCGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGcc                                                                                                                                                                                            <  1:447034/251‑1 (MQ=255)                                                       aCGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGcc                                                                                                                                                                                            >  2:178849/1‑251 (MQ=255)                                                          gcgcGGGCAATCCCTGCGGCAGGGGCGCCCGGTTCGTAAAGGCTGCTGGGGATATCGAGGGTGCCGCCTTTGCGCGCGAACCCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGGCGGCAGCAGCTTGCTGAGCGGCACATCCCAGTTTTCCAGCAGGTCATAAATGGG‑CTGTAACGCCATTGTACTG‑TCGCCCATGT‑GGAACGCGGTGACGCCAGGTTGACCGCCGAGCAAACCGCCAAc                                                                                                                                                                                         <  2:247335/251‑1 (MQ=255)                                                               ggCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCggg                                                                                                                                                                                    >  2:476568/1‑251 (MQ=255)                                                                 cAATCCCTTCGGCAGGGGCGACCGGTTCGTCAGTGCTGCTGGTGATATCGAGGGTGCCGCCTTTGCGCGCGAACGCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGGGGGTCGGCAGCAGCTTGCTGGTCGGCACAGCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAGCACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAGCGCGGGAt                                                                                                                                                                                  <  2:197951/251‑1 (MQ=255)                                                                  aaTACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGAtt                                                                                                                                                                                 >  2:404805/1‑251 (MQ=255)                                                                  aaTACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGAtt                                                                                                                                                                                 <  1:389974/251‑1 (MQ=255)                                                                          cGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTgccgcc                                                                                                                                                                         >  1:21637/1‑251 (MQ=255)                                                                           ggCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTgccgcc                                                                                                                                                                         >  1:419436/1‑250 (MQ=255)                                                                              aGGGGCGACCGGTTCGTAAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCatat                                                                                                                                                                     >  1:412803/1‑251 (MQ=255)                                                                                gggCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCACACCGCCAACGCGGGATTCTGCCGCCCTATTg                                                                                                                                                                   >  2:224665/1‑251 (MQ=255)                                                                                gggCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGGACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCGGCCGCCATATTg                                                                                                                                                                   >  2:380053/1‑251 (MQ=255)                                                                                    gACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGCTTCTGCCGCCATATTGGcc                                                                                                                                                                >  2:479214/1‑250 (MQ=255)                                                                                    gACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGcc                                                                                                                                                                >  1:146645/1‑250 (MQ=255)                                                                                    gACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGcc                                                                                                                                                                >  1:132954/1‑250 (MQ=255)                                                                                     aCCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATGGGCCAg                                                                                                                                                              <  1:132564/251‑1 (MQ=255)                                                                                         gTTCGTCAATGCTGCTGGTGATATCGAGGGTGCCGCCTTGGCGGGCGAACGCCAGCGCCTGCTCAAACAACGGTACGTGGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACAGCGCAGTTTTCCAGCAGGGCATAAATAGG‑CTGGAACGCCATTTGGCTG‑GCGCCCAGGT‑GGAACACGGTGACGCCAGGTTTACCGCCGGTCAAGCGTCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGa                                                                                                                                                          <  1:265807/251‑1 (MQ=255)                                                                                            cgtcaatcctccgggtgtaagcGATGGTCCCCCCTTTGCGCGCGAACTCCAGCGCCTGCTAAAACAACGGTACGTTGCGGTTACCTTGGGTCTGCAGCGGCTGCCTGATCGGCACACCGCGGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTCCTG‑TCGCCCATGG‑GGAACACGGTGCCGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATaa                                                                                                                                                       <  2:84987/230‑1 (MQ=255)                                                                                             gTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATaa                                                                                                                                                       >  1:290512/1‑250 (MQ=255)                                                                                              tCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTAAACCAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAAc                                                                                                                                                     >  2:245523/1‑251 (MQ=255)                                                                                               cAATGCTGCTGGTGATCTCGATGGAGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAACTCGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCCGGTTTACCGCCGAGCAAACCGCCACCGCGGGATTCTGCCGCCATATTGGCCCGGTgctacacg                                                                                                                                                    >  2:270022/1‑244 (MQ=255)                                                                                                 aTGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTc                                                                                                                                                  <  1:201328/251‑1 (MQ=255)                                                                                                  tgctgcTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTACAACGCAAGCTCAAACAACGGTACGTTGCGGATCCCGTGGGTCGGCAGCACCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATCTTGGCCAGGTGATAAACGTcc                                                                                                                                                 >  1:240831/1‑251 (MQ=255)                                                                                                   gctgctGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTcc                                                                                                                                                 <  1:155466/250‑1 (MQ=255)                                                                                                     tgctgGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGACATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGATCAAACCGCCAACGCGGGCTTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGt                                                                                                                                              >  2:23315/1‑251 (MQ=255)                                                                                                       ctgGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGc                                                                                                                                            >  1:403855/1‑251 (MQ=255)                                                                                                       ctgGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGc                                                                                                                                            >  1:149568/1‑251 (MQ=255)                                                                                                                 cGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACgg                                                                                                                                  <  1:176568/251‑1 (MQ=255)                                                                                                                   aTGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCCGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGGGTTAAACGTCCGGt                                                                                                                                              >  2:479285/1‑237 (MQ=255)                                                                                                                   aTGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAAGAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGt                                                                                                                                              <  1:479285/237‑1 (MQ=255)                                                                                                                     ggTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCACACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGGAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGAt                                                                                                                              >  2:114026/1‑251 (MQ=255)                                                                                                                      gtgcgcccTTTGTGCGAGACCTCCAGCGCCTGCTCAAACACCGGTCCGTTGCGTTCAACGTGGTGCGGCAGCATCTTGCTGACCGGCACATCGCATTTTCCCAGCAGGCCATAAATAGG‑CTGAAACGCCTTTTTACTG‑TCGCCCATGT‑GGAACTCGGGGCCGCCGGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATc                                                                                                                             <  2:401451/245‑1 (MQ=255)                                                                                                                        cccgccTTTTCGCGCGACCTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTATCGTGGGTCGGCACCAGCTTGCTGCTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCTTTTTACTG‑TCGCCCGTGT‑GGAACCGGGTGACGCCAGGTTTCCCGCCGAGAAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCTAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCag                                                                                                                           <  1:506352/250‑1 (MQ=255)                                                                                                                        cccgccTTTGCGCGCGAATCCCAGCGCCGGCTAAAACAACGGTAGGTTGCGGTTAACGGGGGTCGGCAGCAGCTTGCTGTTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CGGTAACGCCATTTTACTG‑CCCCCCATGG‑GGAACCCGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCag                                                                                                                           <  2:126116/250‑1 (MQ=255)                                                                                                                                tccgcgcgAACTCCAGCGCCGGCTCAAACAACGGTACGTGGCGGTTAACGTGGGGCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTACCGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTCCCGCCGAGAAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGgc                                                                                                                    <  2:146645/248‑1 (MQ=255)                                                                                                                                      cgAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGT‑CTGTAACGCCATTTTACTG‑GCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTc                                                                                                             <  1:320159/251‑1 (MQ=255)                                                                                                                                       gAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTACCGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTCCTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGAGGGATTCTCCCGCCATATTGGGCAGGTGATAAACGTACGGGGCGGCAGAACGGTGATCAGAGATGGCGCATTTc                                                                                                             >  1:506372/1‑250 (MQ=255)                                                                                                                                        aaCTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCAc                                                                                                           <  1:224665/251‑1 (MQ=255)                                                                                                                                              aGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGGGATGGCGCATTTCACGCCAAt                                                                                                     >  1:75698/1‑251 (MQ=255)                                                                                                                                                    tGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACAcg                                                                                               >  1:417147/1‑251 (MQ=255)                                                                                                                                                     gCTCAAACAACGGTCCGTTGCGGTTAACGTGGGTCGGCAGCAGCTTCCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGGCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACAcga                                                                                              <  1:392073/251‑1 (MQ=255)                                                                                                                                                       ccaaccaagggTACTTTGCGGTGAACGTGGGTCGGCAGCAGCTTTCTGAGCGGCACATCGCAGTTTCCCAGCAGGTCAAAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAGCGGGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACGcgatc                                                                                            <  2:500368/242‑1 (MQ=255)                                                                                                                                                         aaaCAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCCGATCGGCACATCGCAGTTTTCCAGCCGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACAcgatcg                                                                                           >  1:252867/1‑250 (MQ=255)                                                                                                                                                          aaaaacGTTACGTTGCAGTTAACGTGGGTCGGCAGCATCTGGCTGTGCGGCACAGCGCATTTTCCCAGCAGGTCA‑AAATAGGGCTGAAACGCCATTTAACTG‑GCGCCCATGG‑GGAACACGGGGACGCCAGGTTTCCCGCCGAGCAAACCGCCACCGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACAcgatcgat                                                                                         <  2:435315/248‑1 (MQ=255)                                                                                                                                                           acaacGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACAcgatcgat                                                                                         >  1:412763/1‑250 (MQ=255)                                                                                                                                                              acGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATc                                                                                     >  1:176843/1‑251 (MQ=255)                                                                                                                                                                ggTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGc                                                                                   >  1:59261/1‑251 (MQ=255)                                                                                                                                                                ggTACGTGGCGGTTAACGGGGGTTGGAGGCAGCTTGCGGTTCGGAACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑CCGCCCATGT‑GGAACACGGGGCCGCAAGGTTAACCGCCGAGCAAACCGCAAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGc                                                                                   <  2:20172/251‑1 (MQ=255)                                                                                                                                                                 gTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGcc                                                                                  >  2:396164/1‑251 (MQ=255)                                                                                                                                                                   aCGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAACAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCAc                                                                                >  1:295633/1‑251 (MQ=255)                                                                                                                                                                      ttGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTc                                                                             >  1:41205/1‑251 (MQ=255)                                                                                                                                                                          ggTTAACTTGGACCGGCAGCAGCTTGCTGATCGGCCCATACCAGTTTTACAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TAGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCCACGCGGGATTCTGCCGCCCTATTAGCCAGGAGATAAACGTCCGGTGCGGCCGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCCCACGAGCGATCATCGCCCCGTCtttt                                                                         >  2:35090/1‑251 (MQ=255)                                                                                                                                                                           gTTAACGTGGGCCGGCAGCAGCTTGCTGAGCGGCCAACCGCAGTTTTCCAGCAGGTAATAAATAGG‑CGGTAACGCCATTTTCCTG‑TCGCCCATGG‑GGAACACGGTGACGCCAGGTTTCCCGCCGAGCAACCCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCttttt                                                                        <  2:132954/251‑1 (MQ=255)                                                                                                                                                                              aaCGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTcc                                                                      >  1:292821/1‑250 (MQ=255)                                                                                                                                                                              aaCGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGAGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTcc                                                                      >  2:375941/1‑250 (MQ=255)                                                                                                                                                                                        ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGt                                                           >  1:250378/1‑251 (MQ=255)                                                                                                                                                                                        ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCACATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGt                                                           >  1:489769/1‑251 (MQ=255)                                                                                                                                                                                               gcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑GCGCCCATGT‑GGAACCCGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTg                                                    <  1:245523/251‑1 (MQ=255)                                                                                                                                                                                                   gCTGATCGCCAAACCGCAGTTTCCCAGAGGGTCATAAATGGT‑CTGTACCGCCATTTTCCTG‑CCGCCCTTGT‑GGAACGCGGTGACGCCGGGTTAACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCTCGTAACCCGTAATGTTGCggg                                                <  2:489769/251‑1 (MQ=255)                                                                                                                                                                                                    cTGATCGGCACATCGCAGTTGTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCCGGTTTACCGCCGAGCAAACCGCCAACGCGGGCTTCTGCCGCCCTATTGGCCAGGTGATCAACGGCCGGTGCGGCAGCACGGTGATCAGAGATGGCGCATTTCACGCCCATCACACGATCGCAATTCGCCACGTCTTTTTCCACGGAACCCGTTATGGTGCggg                                                >  2:444936/1‑250 (MQ=255)                                                                                                                                                                                                      gATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTTTAACGCCTTTTTACTG‑TCGCCCATGT‑GGAACAGGGTGCCGCCAGGTTTACCGCCGAGCAACCCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGa                                               <  1:90153/249‑1 (MQ=255)                                                                                                                                                                                                        ccgccacaTCGCAGTTTTCCAGCAGGTCATAAATGGG‑CTGAACCGCCATTTTACTG‑TCGCCCATGT‑GAAACACGGTGCCGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAAGCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAggg                                           <  2:18054/247‑1 (MQ=255)                                                                                                                                                                                                          gcccccacacAGTTTCCCAGCAGTTAATAAATAGG‑TTGTA‑CGCCCATTTGTTGGTCGCCCAGGT‑GAACCACGGTGGCGCCGGTTTTCCCGCCGAGCAACCCGCCAACCCGGGAGTCTGCCGCCATATTGGCCAGGTGAGAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGAGCGATAAGCGCCACGTCTTTTTCCACGGAACTCGTAATGGTGCGGGAAGGGAc                                         <  2:312684/242‑1 (MQ=255)                                                                                                                                                                                                                ccGCAGTTTTCCATGATGTCAGAAAGAGG‑CTGTAACGCATTTTTACTG‑TGGCCC‑TGTTGGAACAGGGTGACGCCATGTTGACCGCCGAGCAAACCGCAAACTCTTGATTCTGCCGCCATATTGGCCAGGCGATAATCTTCCGGTGCGGCAGAACGGGGATCAGGTATTGCGCATTTCTCGCCAAGCACTTGATCGATAAGCGCTTCGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATa                                   <  1:480752/250‑1 (MQ=255)                                                                                                                                                                                                                  gCAGTTTTCCAGCAGGTAATAAAGAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAg                                 <  1:479214/251‑1 (MQ=255)                                                                                                                                                                                                                   cAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAgc                                <  1:396164/251‑1 (MQ=255)                                                                                                                                                                                                                    aGTTTCCCAGCAGGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAgcg                               <  1:23315/251‑1 (MQ=255)                                                                                                                                                                                                                              cagGTCATAAATAGG‑CTGTAGCGCTATGTTACGG‑TCGCCCATGT‑GGAACACGTTGGCGCCGGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATGGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAGGCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCa                     <  1:353895/251‑1 (MQ=255)                                                                                                                                                                                                                              cagGTCATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGACCATGT‑GGACCACGGTGACGCCAGGCTTCACGCCGAGCAAACCGGAAACGCGGGATTCTGCCGCACTATTGGCAAAGCGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGACAATCACACGATAGATAATCGCCCCGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGc                      >  1:462543/1‑250 (MQ=255)                                                                                                                                                                                                                               ggttaaaaaagaGG‑CTGTAACGCCTTTTTACGG‑TCCCCCATGG‑GGAACACGGGGACGCCAGGTTTCCCGCCGAGCAAACCGCCACCGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCAt                    <  2:21637/240‑1 (MQ=255)                                                                                                                                                                                                                                   cATAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATcc                  >  2:371011/1‑249 (MQ=255)                                                                                                                                                                                                                                    aTAAATAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTCCCGCCGAGCAAACCGCCCACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCCTCCAgg               >  2:255744/1‑251 (MQ=255)                                                                                                                                                                                                                                      aaaTAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGgcg             >  1:378078/1‑251 (MQ=255)                                                                                                                                                                                                                                        aTAGG‑CTGTAACGCCATTTTACTG‑TCGCCCATGG‑GGACCACGGTGCCGCCAGGTTTACCGCCGCGCAAACCGCCAACGCGGGATTCTGCCACCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACAATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGGCGCt           >  1:500481/1‑251 (MQ=255)                                                                                                                                                                                                                                         tAGG‑CTGTAACGCCTTTTTACTG‑TCCCCCATGG‑GAAACACGGGGACGCCAGGTTTCCCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATCTTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGGCGCt           <  2:419436/250‑1 (MQ=255)                                                                                                                                                                                                                                         atggtgtgaatgcccTTTTTACTG‑TGCCCCATGG‑GAAACAGGGTGATGCCAGGTTTACCGCCGAGTAAACCGCCAACGGGGGATGCTGCCGCGATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGATATGGCGCAGTTGACGCCAAGCACACGATCGATAATCGCCTCGTCGTTTCCCACGGAACCCGTAATGGTGCGGGAAGGGACATGAGAAGCGCCGGTCAGCAGCCAGGCGCt           <  2:240831/238‑1 (MQ=255)                                                                                                                                                                                                                                           gg‑CTGTAACGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGGCGCTGAt        >  1:7162/1‑251 (MQ=255)                                                                                                                                                                                                                                                  aaCGCCATTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGACAAGCGCCGGTCAGCATCCAGGCGCTGATGCcttc  >  2:134858/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       CTACCGTTGCCGTCGGAGCTGAGGGTGACGCGTGCCAGCGGAATACCCGCCTGAACGGCGCGGGCAATACCTTCGGCAGGGGCGACCGGTTCGTCAATGCTGCTGGTGATATCGATGGTGCCGCCTTTGCGCGCGAACTCCAGCGCCTGCTCAAACAACGGTACGTTGCGGTTAACGTGGGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGG‑CTGTAACGCCTTTTTACTG‑TCGCCCATGT‑GGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGATTCTGCCGCCATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTC  >  NC_000913/4558661‑4559151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑