Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,235,651	T→C	intergenic (+244/‑255)	pgi → / → yjbE	glucose‑6‑phosphate isomerase/uncharacterized protein YjbE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,235,651	0	T	C	90.0%	26.7 / NA	10	intergenic (+244/‑255)	pgi/yjbE	glucose‑6‑phosphate isomerase/uncharacterized protein YjbE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (3/6);  minor base A (1/0);  total (4/6)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

CGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGG  >  NC_000913/4235412‑4235897                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                       cgtcgATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAg                                                                                                                                                                                                                                              <  1:216508/250‑1 (MQ=255)  gtcgagaggTAGGCCGGATAAGGCGTGCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAGTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAAGTTAACGGAAATCATACCGTGAGGGTAACCCGAAAATAGAt                                                                                                                                                                                                                                            <  2:437997/243‑1 (MQ=255)     accagggaGGCCGGATAAGGCGTTCACGCCGCAGCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGAtttt                                                                                                                                                                                                                                         <  2:168975/244‑1 (MQ=255)                                                   gCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAAGACGCAGATTAATTGCTTTTGtt                                                                                                                                                                                           <  1:143490/251‑1 (MQ=255)                                                                               aGGCCTACAGGTCGATGCCGCTATGTACCTAGTATTCGCCACTTGATACATAGAACGATTGATTACATAACCTTAATAACCCTGCCGACGTTATGTAGGCATTTTTTTATCAGTTAAATCCACTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAAACCTAAAATCGATTTTTAATCGTTGTTTATTTCGGAAAATAAGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGt                                                                                                                                                               >  2:488724/1‑251 (MQ=255)                                                                                                             cGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTg                                                                                                                                 >  2:134352/1‑251 (MQ=255)                                                                                                                                                                                                                                       tGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCattcattc           <  1:15427/247‑1 (MQ=255)                                                                                                                                                                                                                                       tGAGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCattcattc           >  2:15427/1‑247 (MQ=255)                                                                                                                                                                                                                                       gaaGGTTAACCCTAAAATAGATTTTTAATCGTTGTTTATTGCGGAAAATACGCAGATTAATTGCTTGTGTTTTTATTTTAAGTTTAAGGTTTTTATTGTTATTTAAATATAAGTTGAAACGTATATTTTATATTCAGTCCAAGTATCCTAAAACGCTAACGCTAAGTCCCCGCGCCTTAATTCGCATGCTGTAGTTGTGTATACGCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATg       <  1:488724/249‑1 (MQ=255)                                                                                                                                                                                                                                            ttAACCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAATTATAAGTTGATACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATAGCTAATTCCCCGCGCCGTATTTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGACATGTTTTTGGGTAAATCTCCATTCATTCAATGAAggg  >  2:187103/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                       CGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAATAACCTTAATAACAATGCCGACGTTATGTCGGCATTTTTTTATCAGATAAATCCCCTTGTCTGTAATTTAACGGAAATCATACCGTGAGGTTAATCCTAAAATAGATTTTTAATCGTTGTTTATTTCGGAAAATACGCAGATTAATTGCTTTTGTTTTTATTTTAAGTTTATGATTTTTATTGTTATTTAAATATAAGTTGAAACTTATATTTGATATTCATTCCAATTATCCTAAAACGCCATCGCTAATTCCCCGCGCCGTAATTCGCATGCTTTAGTTGTGTATACTCGATCCCGCCCGAAATGTTTTTGGGTAAATCTCCATTCATTCAATGAAGGG  >  NC_000913/4235412‑4235897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑