Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,926,772	C→A	G155G (GGC→GGA)	ppnN →	nucleotide 5'‑monophosphate nucleosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,926,772	0	C	A	100.0%	212.0 / NA	65	G155G (GGC→GGA)	ppnN	nucleotide 5'‑monophosphate nucleosidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (35/30);  total (35/30)

CTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCAT  >  NC_000913/2926527‑2927011                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                cTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTc                                                                                                                                                                                                                                             >  2:1956/1‑250 (MQ=255)    gAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTc                                                                                                                                                                                                                                         <  2:100684/251‑1 (MQ=255)         gATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAAtt                                                                                                                                                                                                                                     >  1:374136/1‑250 (MQ=255)          agtAATCCCCCGGAAGAGGCTTTTTTCGAGGGGCGAATTATTCGCGCTTTGCAGGTCAACTTGTTCGCGGTCCTGCGTGACAGTCGCTTCGTTGACGGGCAAATCCATAACTCCGTTCTGTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACGAACCTGGTCTTTGCCATCTTGCGTAACGCTCGCGCGCTGCACGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGTGAATGTCACTCAATTaa                                                                                                                                                                                                                                   <  2:324362/249‑1 (MQ=255)             aaTCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACg                                                                                                                                                                                                                                 >  1:86187/1‑250 (MQ=255)                 ccccGGAAGAGGCTTTTGTCGATGGACGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGGCTGCTGGGGAGGTCACTCAATTAACGaaacc                                                                                                                                                                                                                            <  2:282424/251‑3 (MQ=255)                         gagGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGAAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGCTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCCCTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAACTATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTAttt                                                                                                                                                                                                                    >  1:205196/1‑251 (MQ=255)                          agGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTg                                                                                                                                                                                                                   >  1:93326/1‑251 (MQ=255)                               tttGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTCTTCGCGGTCCTGCGTGACATTCTCTTCGTTTCCGGGCACATCCATCACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCACTTCACGAAACCGAGTATTTGTATGc                                                                                                                                                                                                              >  2:262364/1‑251 (MQ=255)                                  gTCGATGCGCGACTTCTTCGCGCTTTGCCGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCACATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCACATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCcg                                                                                                                                                                                                           >  2:270957/1‑251 (MQ=255)                                    cGATGGGCGAATTATTCGCGCGTTGCAGGCCAACTTGTTCGCGGGCCTGCGTGACATGCTCTTCGTTTACGGGCAAATCCATAACTCCGTTCGTTTTCCCTACCTGAAGCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTGCATCTGGCGTAACGCTCGCTCGCTGCATGGGGGTGAAGCGCCAAATATGGTGGTCAGCTGGTGAGGTCACTCAATGAACGAAAACGAGTATTTGTATGCCcgtc                                                                                                                                                                                                         <  1:239730/251‑1 (MQ=255)                                        gggCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCcgtcgcgt                                                                                                                                                                                                     >  2:112389/1‑251 (MQ=255)                                        gggCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCcgtcgcgt                                                                                                                                                                                                     <  1:75427/251‑1 (MQ=255)                                        gggCGAATTATTCGCGCTTTGAGGGCCAACTTGTTCGCGATCCTGCGTGACATTCTCTTCGTTTACGGGCACATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACCACTCCGTCCACATCACTAACCTGGTCTTTTCAATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCcgtcgcgg                                                                                                                                                                                                     >  2:92086/1‑250 (MQ=255)                                                 attCGCGCTTTGCAGGCCCACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCACCCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGACGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTCTTTGAATGCCCGTCGCGTCGGAAAcc                                                                                                                                                                                             >  2:326329/1‑250 (MQ=255)                                                    cgcgCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAGCGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCt                                                                                                                                                                                         >  2:104282/1‑251 (MQ=255)                                                    cgcgCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCt                                                                                                                                                                                         <  1:374952/251‑1 (MQ=255)                                                      cgcTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTgg                                                                                                                                                                                       >  2:293028/1‑251 (MQ=255)                                                      cgcTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTgg                                                                                                                                                                                       >  2:210553/1‑251 (MQ=255)                                                            gCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTGTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGGATGCCCGTCGCGTCGGAAACCAGCTGGGCCTg                                                                                                                                                                                  <  2:234255/250‑1 (MQ=255)                                                                        gTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGact                                                                                                                                                                      >  2:277761/1‑248 (MQ=255)                                                                        gTTCGCGCTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAat                                                                                                                                                                      >  2:31630/1‑250 (MQ=255)                                                                                      gTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCtgt                                                                                                                                                       >  1:30931/1‑251 (MQ=255)                                                                                      gTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCtgt                                                                                                                                                       <  2:127056/251‑1 (MQ=255)                                                                                          cATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTc                                                                                                                                                   <  1:358883/251‑1 (MQ=255)                                                                                                ctTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCGTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCgg                                                                                                                                                <  1:262364/248‑1 (MQ=255)                                                                                                  tCGTTTACGGGCAAATCCATAACCCCGGTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCGTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAAGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAg                                                                                                                                             <  1:92086/249‑1 (MQ=255)                                                                                                    gTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGAt                                                                                                                                         <  1:270957/251‑1 (MQ=255)                                                                                                     tttACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTCAATCTAGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGAGGTCTGCTGGGGAGGTCACTCAATTAACGCAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGCGCGATg                                                                                                                                        >  1:51312/1‑251 (MQ=255)                                                                                                              aaaTCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCg                                                                                                                               >  2:40513/1‑251 (MQ=255)                                                                                                               aaTCCATAACACCGTTGGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGACTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCg                                                                                                                               >  2:143194/1‑250 (MQ=255)                                                                                                                 tCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTAAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGGGCCGATg                                                                                                                            >  1:462037/1‑251 (MQ=255)                                                                                                                  ccATAACACCGGTCGGTTTCCCAACCGTAATCTCGACAACTCCGTCCACATCACTAACCTGGTCGTTTCCATCGTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCTGAAACCAGCGGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGATCGATGGAAGCGCCGATg                                                                                                                            <  2:183821/250‑1 (MQ=255)                                                                                                                        cacCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGtg                                                                                                                     <  1:331945/251‑1 (MQ=255)                                                                                                                         acCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGAt                                                                                                                                         <  1:169009/230‑1 (MQ=255)                                                                                                                         acCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGAt                                                                                                                                         >  2:169009/1‑230 (MQ=255)                                                                                                                         acCGTCCGTTTTCACAACCTGAATATCGACAACTCCGTCCACATCACTCACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATCTGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAACACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGCTGAAAGGtgc                                                                                                                    >  2:246042/1‑251 (MQ=255)                                                                                                                               cgttTGCCCAACCTGAATCGCGACAACTCCTTCCACCGCACTAACCTGGTCTTTTCCATCTTGCGTTACGCTCGCGCGCTGCACGTGGGTGAAGCGCCAAATATGGTGGTCTGCGTGTGAGGTCTCTCAATTAACGAGAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCTTTCGCTGAATATCGGCTCCGGCTTCGGTCCGGGTGTTATGGTAGCTCCGATGAAAGGTGCTGCGGt                                                                                                              <  2:494983/251‑1 (MQ=255)                                                                                                                                             gAATCTCGCCAACTCCGTCACCACCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGt                                                                                                                                                                                                                  >  2:449177/1‑137 (MQ=255)                                                                                                                                             gAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGTTCTGCTGGGGAGGTCACGCAAGTAACGAAAACGAGTATTTGt                                                                                                                                                                                                                  <  1:449177/137‑1 (MQ=255)                                                                                                                                             gAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCgca                                                                                                  <  2:374136/249‑1 (MQ=255)                                                                                                                                                   cGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCAGCTTGCGTAACGCTCGCGCGCTGCATGTGGTTGAAGCGCCAAATATGGGGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCGGAATATCTGCTCCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGGAAGGGGCTGCTGTCGGACACGCCCagcagc                                                                                             <  2:218525/248‑1 (MQ=255)                                                                                                                                                      cAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACa                                                                                       <  1:293028/251‑1 (MQ=255)                                                                                                                                                                      acgcaCCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTTGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTAt                                                                       <  2:127568/247‑1 (MQ=255)                                                                                                                                                                              ggTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTCAAGCGCCCCATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATg                                                                >  1:65069/1‑250 (MQ=255)                                                                                                                                                                               gTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGACACCAGCTGGGCCTGCGTGAGATGAATATCTGCACCGGCTGTGGGCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGCCCCGCGCCGCAGCGTTACCAAGACAGTCGTTTTATTGGTATGAc                                                              >  2:231701/1‑251 (MQ=255)                                                                                                                                                                                     tCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGcc                                                        >  1:387098/1‑251 (MQ=255)                                                                                                                                                                                      ccATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCg                                                       >  1:377254/1‑251 (MQ=255)                                                                                                                                                                                         tCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCg                                                    >  1:419479/1‑251 (MQ=255)                                                                                                                                                                                            tGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGAt                                                  <  1:210553/250‑1 (MQ=255)                                                                                                                                                                                             gCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGAtt                                                 >  1:10492/1‑250 (MQ=255)                                                                                                                                                                                               gTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATc                                              >  1:40469/1‑251 (MQ=255)                                                                                                                                                                                                   cGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATcgccg                                          >  2:411587/1‑251 (MQ=255)                                                                                                                                                                                                    gCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGACGCGCCGATGAAAGGTGCTGCGGTCGGAACCGCGCAGCCGCGTTACGAAGCCAGTCGTTTTATTGGTATGACAGAGCCGTCGCTTATcgccgc                                         >  2:23964/1‑251 (MQ=255)                                                                                                                                                                                                    gCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGGTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATcgccgc                                         <  1:101153/251‑1 (MQ=255)                                                                                                                                                                                                       cgcgcgCTGCTGGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACTAGTATTTGTATGCCCGTCGCGTCTGAAACCAGCTGGGCCTGCGTGAGCGGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTTCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCt                                        <  1:326329/249‑1 (MQ=255)                                                                                                                                                                                                       cgcgcgCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTAt                                               <  1:132459/242‑1 (MQ=255)                                                                                                                                                                                                       cgcgcgCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGGGCTGCGGTCGGACACGCGCCGCCGCGTTACAAAGACAGTCGTTTTATTGGTATGACCGAGCCGTCGATTAt                                               >  2:132459/1‑242 (MQ=255)                                                                                                                                                                                                                    tgGGTGAAGCGCCACATATGGTGGTCTGCTGGGGAGGCCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGAAGCAGCGTTACAAAGACAGTCGTTTTATTGGTTTGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCg                         >  1:166190/1‑251 (MQ=255)                                                                                                                                                                                                                           aGCGCCAAATATGGTGGTCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGGCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCGGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCa                  <  2:151806/251‑1 (MQ=255)                                                                                                                                                                                                                             cgcCAAATATGGTGGTCTGCTGGGTAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGTGCCTTCGTGAGCTGAATATCTGCACCGGCTGTGTTCCGGGAGCGATGGAAGCGCCGATGAATGGTTCTGCGTTCGTACACGCGCAGCAGCGTTACAAAGACAGGCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAAc                <  1:209409/251‑1 (MQ=255)                                                                                                                                                                                                                                  aaTAGGGTGGTCTGCTGGGGAGGTCCCTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAAtt           <  2:30931/251‑1 (MQ=255)                                                                                                                                                                                                                                       ggtggtCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTTTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGGCCGGGAGCGATGGAAGCGCCGATGAAATGTGCTGCGGGCGGTCGCTCGCGGGTGCGTTACAAAGACAGTCGTTTTATGGGTATGACAGAGCGGTCGGGTTTCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGatca      <  1:246042/251‑1 (MQ=255)                                                                                                                                                                                                                                          ggtCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGatcatc    <  1:31630/250‑1 (MQ=255)                                                                                                                                                                                                                                           gtCTGCTGGGGAGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATcatcat  >  1:439552/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                CTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCAT  >  NC_000913/2926527‑2927011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑