Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,652,331	T→C	intergenic (+2346/+397)	ydfD → / ← ynfP	lysis protein/protein YnfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,652,331	0	T	C	100.0%	129.8 / NA	34	intergenic (+2346/+397)	ydfD/ynfP	lysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/17);  total (17/17)

ACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACC‑AATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGC‑AAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCATGC  >  NC_000913/1652106‑1652575                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     aCTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACC‑AATTGGGGCCTTTATCTTTTTAAGCCCGCCAACGACGCTCCCCGGAGGCAGATGACATTAACGAGACTGAGC‑AAAAAACAAATCATTGTTGTTCACCTCCGAGAGTATGGAagaaaagaaa                                                                                                                                                                                                                              >  2:475556/1‑250 (MQ=255) aCTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACC‑AATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGC‑AAAGAGCTTATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGaaa                                                                                                                                                                                                                              <  2:404531/250‑1 (MQ=255) aCTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACC‑AATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGC‑AAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGaaa                                                                                                                                                                                                                              <  1:18370/250‑1 (MQ=255)    ggAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACC‑AATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGC‑AAAGAGCATATCATTGATGTTCGCCTCAGAGAGTATGGCAGAACAGaaaaa                                                                                                                                                                                                                            <  2:293875/249‑1 (MQ=255)     gAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACC‑AATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCCGATGACATTAACGAGACTGAGC‑AAAGAGCATAACATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACa                                                                                                                                                                                                                          >  1:492208/1‑250 (MQ=255)          cGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACC‑AATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGC‑AAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAAt                                                                                                                                                                                                                     <  2:350217/250‑1 (MQ=255)                ggTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACC‑AATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGC‑AAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCtt                                                                                                                                                                                                              <  1:402179/251‑1 (MQ=255)                     tccgatcccACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACC‑AATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGC‑AAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGtt                                                                                                                                                                                                          <  1:514680/242‑1 (MQ=255)                      cTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACC‑AAACGGGTCATTCATCTTATTAAGCCAGCAAACGCAGCTCCCCGGAGGCAGAGGACATTAAAGAGGATGAGA‑AAAGAACATTTCATTGATGTTTACCCCAAGGAGGATTGCAGAACAGAAAAACAAAAAAGAACCTTTGtttt                                                                                                                                                                                                        >  2:317246/1‑251 (MQ=255)                             cACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAAAACAGGCGGGAACCCAACCGGG‑AATTCATCTTTTTAAACCACCAATCCGCGCACACCCGAGGCAGCTGACATTTAAGAGAATGAGCCAAACACAATA‑CATTGATGTTTAACTCAGAGGGTAAGGCAGAAAAGAAAAACCAAAATACAACTttttttgaatggg                                                                                                                                                                                                  >  2:514680/1‑243 (MQ=255)                                       ggaggaCATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACC‑AATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGC‑AAAGAGCATATCATTGATGTTCACCGCAGAGAGGAGGGCAGAACAGAAAAACAAAAATGCACCTTGGTTGTTCAACCTGAAGGGTCAg                                                                                                                                                                                       <  2:492208/251‑1 (MQ=255)                                        gaggaCATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACC‑AATCGGTTCATTCATCTTATTAAGCCAGCAAACGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGC‑AAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTGTGTTTTTCAACCTGAAGTGTCAGc                                                                                                                                                                                      <  1:365757/251‑1 (MQ=255)                                                             ggAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACC‑AATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGC‑AAAGAGCAGATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTg                                                                                                                                                                  <  2:499036/250‑1 (MQ=255)                                                              gAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACC‑AATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGC‑AAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGAc                                                                                                                                                                <  1:295313/251‑1 (MQ=255)                                                                        gatCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAATC‑AATCGGGGCATTCATATTATTAAGCCAGCAATCGACGCTCTCCGGAGGCAGATGACATTAAAGAGAATGAGC‑AAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGGACAGAAAAACAAAAAAGGCCCTTTGTTTTTCAACCTGAAATGTCCACGAGAGGAAAAAATTATGGGGACCATTTTACCg                                                                                                                                                      >  2:390040/1‑251 (MQ=255)                                                                              gagaAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACC‑AATCGGGTCATTCCTCTTATTAATCCCGCCATCGCAGCTCTCCGGAGTCAGATGACAATTACGAGACTGAGC‑AAAAAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAAAGCACCTTTGTTTTTTAACCTTAAGTGTCAGCGAGGGTAAAAAATTTTGGTGAcagagcgggaaagcgcg                                                                                                                                                >  2:372239/1‑235 (MQ=255)                                                                              gagaAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACC‑AATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGC‑AAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGAc                                                                                                                                                                <  1:372239/235‑1 (MQ=255)                                                                                gaAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACC‑AATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGC‑AAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCa                                                                                                                                              <  1:390040/251‑1 (MQ=255)                                                                                                       aCGGTACAAAAAACACAGGCGGGAACC‑AAACGGGTCATTCATCTTATTAAGACAGCAATCGACGCTCTCCGGAGTCAGATGAAATTAACGAGACTGAGC‑AAAGAGCATATCATTGAAGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAAACTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGAACATTTTACCGTTGTCTCAAAAAGGCAGATGTGGGACGCAGc                                                                                                                       >  1:436416/1‑251 (MQ=255)                                                                                                                                   aaTCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGC‑AAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCg                                                                                            >  2:113172/1‑251 (MQ=255)                                                                                                                                   aaTCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGC‑AAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCg                                                                                            >  2:149962/1‑251 (MQ=255)                                                                                                                                            attcatCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGC‑AAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGc                                                                                                           <  1:425353/227‑1 (MQ=255)                                                                                                                                            attcatCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGC‑AAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGc                                                                                                           >  2:425353/1‑227 (MQ=255)                                                                                                                                             ttcatCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGCGACTGAGC‑ACAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATgg                                                                                                                                                                    >  1:274003/1‑169 (MQ=255)                                                                                                                                             ttcatCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGC‑AAAGAGCATATCATGGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATTCACCTTTGTTTTTCAACCTGAAGTTTCAGCGATAGTAAAAAATTATgg                                                                                                                                                                    <  2:274003/169‑1 (MQ=255)                                                                                                                                                  cTTATTAAGCCAGCAATCGCCGCTCTCCGGAGTCAGATGACCTTAACGAGACTGAGC‑AAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGCCCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATACAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTc                                                                             >  2:215323/1‑251 (MQ=255)                                                                                                                                                   ttattaAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGC‑AAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCgaga                                                                                                                                                                                  >  1:416844/1‑149 (MQ=255)                                                                                                                                                   ttattaAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGC‑AAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCgaga                                                                                                                                                                                  <  2:416844/149‑1 (MQ=255)                                                                                                                                                               cAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGC‑AAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTAt                                                                >  1:127251/1‑251 (MQ=255)                                                                                                                                                                                                  agaCTGAGC‑AAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGc                             >  1:430912/1‑251 (MQ=255)                                                                                                                                                                                                    aCTGAGC‑AAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCAt                           >  1:269436/1‑251 (MQ=255)                                                                                                                                                                                                          c‑AAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAg                     <  1:113172/251‑1 (MQ=255)                                                                                                                                                                                                            aaaGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGc                    >  2:390591/1‑251 (MQ=255)                                                                                                                                                                                                                              gTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCATGc  <  2:127251/251‑1 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     ACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACC‑AATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGC‑AAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCATGC  >  NC_000913/1652106‑1652575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑