Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,227,506	C→A	intergenic (‑34/‑175)	pliG ← / → ycgL	inhibitor of g‑type lysozyme/PF05166 family protein YcgL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,227,506	0	C	A	100.0%	123.1 / NA	32	intergenic (‑34/‑175)	pliG/ycgL	inhibitor of g‑type lysozyme/PF05166 family protein YcgL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/17);  total (15/17)

CAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGGCGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGAT  >  NC_000913/1227259‑1227727                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                              cAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAAATGt                                                                                                                                                                                                                            >  1:424973/1‑251 (MQ=255)     gcCTTCATCAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGATGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGCGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAAATGTtat                                                                                                                                                                                                                         >  2:62772/1‑250 (MQ=255)      ccTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCATTGCAAGCAGCAATACAGCCTTCCTGATGCTCGTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAAATGTtataa                                                                                                                                                                                                                       <  1:263591/251‑1 (MQ=255)           aTTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAAATGTtataatataa                                                                                                                                                                                                                  <  1:287223/251‑1 (MQ=255)                 gaTACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAAATGTTATAATATAACAATcc                                                                                                                                                                                                            >  1:178251/1‑251 (MQ=255)                    aCTCAAATGCTCTTTCTGACCTTGTTTGGCATAGCTGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGGGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACAGTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTCTTTTTCATTGGGAGTCGCCTGTTCAGAATGAGTACGATGAAATGTtataatatccccatccct                                                                                                                                                                                                          <  2:130187/250‑11 (MQ=255)                                                gcagaGAAGGTATATGCAGCGTCATCGTTTCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAAATGTTATAATAGAACAATCCCTAACGGGAGAATTCCCGTGGCTGTCCTGCg                                                                                                                                                                             <  2:290280/247‑1 (MQ=255)                                                    aGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAAATGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCg                                                                                                                                                                         >  2:80312/1‑251 (MQ=255)                                                      aaGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAAATGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGt                                                                                                                                                                       >  1:95053/1‑251 (MQ=255)                                                             atgttacgtaatcgtaTCCCTTTATTTCGGCGGAATGCGGAGCGGTTCTATGGCCTTTTCTGAACCCAACAATGACAATCTTACCCGCAGCAAAGCTCCTGGAGGTTAGCAATTCAAGCAGCAATACAACCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAAATGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATc                                                                                                                                                                 <  1:461150/244‑1 (MQ=255)                                                                 atcgtaatcgtaTCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAAATGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGtt                                                                                                                                                            >  2:416928/1‑251 (MQ=255)                                                                       atcgtaTCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAAATGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGc                                                                                                                                                       >  1:139575/1‑250 (MQ=255)                                                                         cgtaTCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAAATGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCAtt                                                                                                                                                    <  1:416928/251‑1 (MQ=255)                                                                         cgtaTCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTATGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAAATGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCAtt                                                                                                                                                    >  2:359210/1‑251 (MQ=255)                                                                          gtaTCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCAGTGGTAGTCTCCTGTTCAGAATGAGTACGATGAAATGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCAttt                                                                                                                                                   <  2:208960/251‑1 (MQ=255)                                                                            aTCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAAATGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCAtttt                                                                                                                                                  <  2:159270/250‑1 (MQ=255)                                                                                         gCCGGAGTCCTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAAATGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCCTTTTTGCCGGGGGGAtt                                                                                                                                     >  2:34237/1‑250 (MQ=255)                                                                                            ggAGTACTGAGCGCTGCTATGGCCTTTTCTGCACTTACCATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCCGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAAATGTTATAATATATCCATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACtt                                                                                                                                 >  2:479689/1‑251 (MQ=255)                                                                                             gAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAAATGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTta                                                                                                                                <  2:424973/251‑1 (MQ=255)                                                                                                    gAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAAATGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAgg                                                                                                                          <  2:368508/250‑1 (MQ=255)                                                                                                                  ccTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAACGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAAATGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTcaac                                                                                                           >  2:59215/1‑251 (MQ=255)                                                                                                                                accATTGACATTCTTACCCGCAGCAAAGCTCGTTGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAAATGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATc                                                                                             <  1:34237/249‑1 (MQ=255)                                                                                                                                   aTTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCGTGATTTTCAGTGGTAGTCTCCTGTTCAGAAGGAGTACGATGAAATGTTTTAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTAGGTAAAAGCACGGTGCGGCATTTGTGCTGGGGGGATTACTTATAAAGGGGGCATGGACTTAACAAACCGGAGCAATCTAc                                                                                          <  2:331717/251‑1 (MQ=255)                                                                                                                                                cccGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAAATGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCtt                                                                             <  1:215421/251‑1 (MQ=255)                                                                                                                                                           gCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAAATGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGGCGCGGGt                                                                  <  1:106660/251‑1 (MQ=255)                                                                                                                                                                     gTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGAGTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAAATGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGGCGCGGGTTTCATTGATg                                                        <  2:95053/251‑1 (MQ=255)                                                                                                                                                                          cAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAAATGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGGCGCGGGTTTCATTGATGTTAGc                                                   <  1:59215/251‑1 (MQ=255)                                                                                                                                                                               cAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAAATGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGGCGCGGGTTTCATTGATGTTAGCTTATg                                              >  2:128/1‑251 (MQ=255)                                                                                                                                                                                    agcaATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAAATGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGGCGCGGGTTTCATTGATGTTAGCTTATGCCaa                                          >  1:143946/1‑250 (MQ=255)                                                                                                                                                                                                     gATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAAATGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGGCGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTaa                           >  1:469965/1‑248 (MQ=255)                                                                                                                                                                                                      aTGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAAATGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTTGCGCGGGTTGCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAagag                       <  1:359210/251‑1 (MQ=255)                                                                                                                                                                                                                           aGTCTCCTGTTCAGAATGAGTACGATGAAATGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGGCGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGAt  >  2:162607/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                              CAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGGCGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGAT  >  NC_000913/1227259‑1227727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑