Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,196,861	A→G	intergenic (+79/+6)	C0293 → / ← ymfD	small regulatory RNA C0293/putative SAM‑dependent methyltransferase YmfD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,861	0	A	G	100.0%	55.6 / NA	18	intergenic (+79/+6)	C0293/ymfD	small regulatory RNA C0293/putative SAM‑dependent methyltransferase YmfD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/10);  total (8/10)

CACTGTTATTAGGACAATTATGAATTACCACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTAATATTTCAAGGCCATGTGAAGAACATATTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAAT  >  NC_000913/1196633‑1197088                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                    cACTGTTATTAGGACAATTATGAATTACCACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTTCTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGGAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaact                                                                                                                                                                                                               <  2:437594‑M1/251‑18 (MQ=255)  aCTGTTATTAGGACAATTATGAATTACCACTCCTTACACCCGCTCGAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACCGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAACAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactg                                                                                                                                                                                                              >  1:484276‑M1/1‑233 (MQ=255)                          tACCACTCCTTACACCCGCTCAAATATTGTTACATTGCCGGTTTTGTCTCAACTACTCACCCGGGACTCGCCAGGGGCCAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttac                                                                                                                                                                                      >  2:310321‑M1/1‑209 (MQ=255)                                          cGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaaggg                                                                                                                                                                       >  1:127590‑M1/1‑193 (MQ=255)                                                   agtgtTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcggttacaggatgttgaaagggaaaattctgg                                                                                                                                                             <  2:467408‑M1/249‑68 (MQ=255)                                                                    tttGTGTCAACTACTCACCCGGGACTCGCAAGGGGACAGCCAACAGGCATTGAGTGCAATCACCTTAGCGTTCAGGTACATACGGAATGTAAAAAAGGCCGCGCGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACCGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgccgttttcgtttacaggctgttgaaagggaaaattctggggaaaaaaaagcccgcc                                                                                                                                            >  2:303710‑M1/1‑167 (MQ=255)                                                                                actCACCCGGGACTCGCCAGGTGACAGCCAACAGGAATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCTCCTTCACATACATCTTTAGTACTGAGACTTTTTAGCCTAGGGAATTATTGTCGTAccatcaactattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattcgggggcaaaaaaagcccgccagttacggcg                                                                                                                                  <  2:371678‑M1/249‑95 (MQ=255)                                                                                    aCCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaa                                                                                                                             >  1:420346‑M1/1‑151 (MQ=255)                                                                                       cggcactcgCCGGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACAGGCGGAATGGAAAAAAGGCCGCGAGCGCGGCCCTTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtgtacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaacctc                                                                                                                         <  2:31208‑M1/247‑104 (MQ=255)                                                                                                   ggggACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcggggaacctcatcccttcgggg                                                                                                             >  1:61160‑M1/1‑136 (MQ=255)                                                                                                    gggACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTATTACTGAGACTGTTTAACCTAGGGAATTATTGGCGTAccatcaacgattaaactgcgcttcggcgcagttttcggttacagtatgttgaaaggaaaaattctggggcaaaaaaagcccgccatttacgtcgggaaacctcatcctatgggaga                                                                                                            <  2:333460‑M1/251‑117 (MQ=255)                                                                                                      gACAGCCAACCGGCCTCGGGTGCAATAACCTTAGCGTTCAGGTACATGCAGAATGTAAAAAAGGCCGCGACCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggtaaagtcatcctattggagaa                                                                                                           >  1:161967‑M1/1‑133 (MQ=255)                                                                                                             aaCAGGCATTGGGTGCAATCCCCTTAGCGTTCAGTTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaacctcatcctatgggagaacaatgaat                                                                                                   <  2:420346‑M1/251‑126 (MQ=255)                                                                                                                             aaTCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcggggaaccacatcctattggagaacaaaaaaaaattaaattgaggggg                                                                                   >  1:356941‑M1/1‑110 (MQ=255)                                                                                                                                                              aaaaaaGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaacctcatcctatgggataacaatgaataatgaaattgcggggttatcatctcccagtatatccatactaacaata                                                   <  2:61160‑M1/250‑174 (MQ=255)                                                                                                                                                                                       tCACATACATCTTTAGTACTGAGACTGTTTAACCAAGGGAATTATTGTCGTAccatcaacgaataacctgcgcttcggcgcatttttcgtttacaggatgttgaaagggaaaattctggggaaaaaaaagcccgccagttacggcgggaaacctcatcctatgggagaacaatgaataatgaaattgcggggttatcagctcccagtatatccatactaacaataaggttatttactcaaccaggcataa                          <  2:129075‑M1/250‑199 (MQ=255)                                                                                                                                                                                                           gagaCTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcatttttcttttacaggatgttgaaagggaaaatcctggggcaaaaaaagcccgccagttacggcgggaaacctcatcctatgggagaacaatgaataatgaaattgcggggttatcatctcccagtatatccatactaacaataaggttatttactcaaccaggcataaacattttgttgtgtgcgtgg      <  2:431515‑M1/250‑219 (MQ=255)                                                                                                                                                                                                              aCTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaacctcatcctatgggagaacaatgaataatgaaattgcggggttatcatctcccagtatatccatactaacaataaggttatttactcaaccaggcataaacattttgttttgtgcgtgggaac  <  2:127590‑M1/251‑223 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                    CACTGTTATTAGGACAATTATGAATTACCACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTAATATTTCAAGGCCATGTGAAGAACATATTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAAT  >  NC_000913/1196633‑1197088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑