Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	670,635	A→T	L324Q (CTG→CAG)	holA ←	DNA polymerase III subunit delta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	670,635	0	A	T	100.0%	227.2 / NA	60	L324Q (CTG→CAG)	holA	DNA polymerase III subunit delta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (33/27);  total (33/27)

CAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCAGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAACAACAGTTCACGTTGTAATGTGCGCAACAAAATAACCGGTT  >  NC_000913/670392‑670881                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       cAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGATGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGc                                                                                                                                                                                                                                                 >  1:268913/1‑251 (MQ=255)          gtTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACactga                                                                                                                                                                                                                                        <  1:465911/251‑1 (MQ=255)            ttACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACactgac                                                                                                                                                                                                                                       <  1:526234/250‑1 (MQ=255)             tACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACactgactg                                                                                                                                                                                                                                     >  1:61273/1‑251 (MQ=255)                   gCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGTTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTGCGCCAGGGGTTTATGGCACAACATAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTa                                                                                                                                                                                                                               <  1:357119/251‑1 (MQ=255)                     ccGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACGGACTGACCGTAAt                                                                                                                                                                                                                             <  2:81826/250‑1 (MQ=255)                      cGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATc                                                                                                                                                                                                                            <  1:276639/251‑1 (MQ=255)                                  ccGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGt                                                                                                                                                                                                                <  1:162856/251‑1 (MQ=255)                                        ggggACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTc                                                                                                                                                                                                          >  1:352922/1‑251 (MQ=255)                                                  cGGAGGAACATTATTAGGGATGATCGTGACCCGCGTCAGACCAATCACATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACCGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCCGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGCGGGTGAGTTCCGTCCGTGTc                                                                                                                                                                                                >  2:201039/1‑251 (MQ=255)                                                     aggaACATTATTAGGGATAATAGTGACCCGCGTCAGAACAATCAAATTCGTCAGCGTTTCCGCGGGTTTTAGATGACCATAGTGCACGGGATAAAAGGTGCTGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACCGAAGAGATAACCTTTCCTGCTCTGCCAACACTGACTGACAGTAATCTTGTTTGAGGTCGAGTTGCGTTCGTGTCAgg                                                                                                                                                                                             >  2:240131/1‑251 (MQ=255)                                                          cATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGTCCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTg                                                                                                                                                                                        >  2:412972/1‑251 (MQ=255)                                                           attattAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGc                                                                                                                                                                                       >  2:519595/1‑251 (MQ=255)                                                                     atgatTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGAc                                                                                                                                                                             <  1:241624/251‑1 (MQ=255)                                                                     atgatTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCGTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGAc                                                                                                                                                                             <  1:485723/251‑1 (MQ=255)                                                                             gACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGc                                                                                                                                                                     <  1:113603/251‑1 (MQ=255)                                                                                 cgcgTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCt                                                                                                                                                                 >  2:57060/1‑251 (MQ=255)                                                                                  gcgTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGGGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATATCCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGTTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACGGCGTCTg                                                                                                                                                                <  2:218565/251‑1 (MQ=255)                                                                                    gTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGCCCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGCCGTAACTGCGTCTGAc                                                                                                                                                              >  2:50762/1‑251 (MQ=255)                                                                                      cAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTGAGAGGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAAGCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACtt                                                                                                                                                            <  1:482303/251‑1 (MQ=255)                                                                                          ccAATCAAATTCGCCAGCGTTTCAACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGc                                                                                                                                                        >  2:80914/1‑251 (MQ=255)                                                                                               cAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGAttt                                                                                                                                                   <  1:64691/251‑1 (MQ=255)                                                                                                      gCCAGCGTTTCCAACAGTTTTGGATGACCATTGTGCACCGGATGATAGGCGCCGGCAAACAGAGCCTGTCGAGATCTCCTATCAACCGTCGCTAAATACCTCCCCCCGGGGTTAATGGCACAACAGACGAGCTAACCCTTCCTGCACTGCCCACACTGCCTGACCGAAATCTTGTTTGCGGGTCAGTTCCGTTCGTGTCAGGCGTTGAACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTATCGccc                                                                                                                                            >  2:479973/1‑250 (MQ=255)                                                                                                      cccAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCt                                                                                                                                            <  1:386705/250‑1 (MQ=255)                                                                                                         aGCGTTTCCACGGGATTTAGATGCCCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGCAAAGCTTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTACCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGTTGAGTTCCGTTCGTGTCAGGAGCTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGc                                                                                                                                         >  2:265265/1‑251 (MQ=255)                                                                                                             tttCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCcat                                                                                                                                     >  1:100502/1‑251 (MQ=255)                                                                                                              ttCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCcatc                                                                                                                                    >  1:20953/1‑251 (MQ=255)                                                                                                                    gggTTTTAGATGTCCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGccc                                                                                                                              <  1:412972/251‑1 (MQ=255)                                                                                                                    gggTTTAAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGccc                                                                                                                              <  2:262570/251‑1 (MQ=255)                                                                                                                                 ccATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGcc                                                                                                                 >  1:218083/1‑251 (MQ=255)                                                                                                                                    tAGTGCACCGGATCAAAGGTGACGCCACACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCAt                                                                                                               >  2:509007/1‑250 (MQ=255)                                                                                                                                        gCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACTGCCTGACGTAACTGCGTCTGACTTAAGCGATGTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCg                                                                                                          <  1:57060/251‑1 (MQ=255)                                                                                                                                         cACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCg                                                                                                          >  1:350800/1‑250 (MQ=255)                                                                                                                                            cGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCGCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAGCTGCGTCTGACTTAAGCGATTTATCGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGc                                                                                                      <  1:80914/251‑1 (MQ=255)                                                                                                                                                   aaGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGccc                                                                                                                                                                                                                                               <  1:281253/107‑1 (MQ=255)                                                                                                                                                   aaGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGccc                                                                                                                                                                                                                                               >  2:281253/1‑107 (MQ=255)                                                                                                                                                   aaGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTCACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGTTGCTTaaca                                                                                                >  2:282058/1‑247 (MQ=255)                                                                                                                                                        gccgccACACAGCGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCCTACCCGATGCTTATCAaaccac                                                                                          >  2:54819/1‑248 (MQ=255)                                                                                                                                                               aaCAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAacgcccgcc                                                                                   >  2:8676/1‑250 (MQ=255)                                                                                                                                                                   gagCCTGTAAAGATTTCATATCAACCGGCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGAGTTAACGCCTCGCCCATCATGCCCCGGCGGTGCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTgg                                                                               <  2:352922/251‑1 (MQ=255)                                                                                                                                                                      ccTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGGGGCGt                                                                            >  2:98998/1‑251 (MQ=255)                                                                                                                                                                            aaGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGc                                                                      >  1:227091/1‑251 (MQ=255)                                                                                                                                                                               aTTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCCGTGGCGTATGGGccg                                                                    >  1:365822/1‑248 (MQ=255)                                                                                                                                                                                    atatCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGc                                                              >  1:244186/1‑251 (MQ=255)                                                                                                                                                                                      atCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTGGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTGAACGCCTCGCCCATCATGCCCCGGCGGGTCTGCCAGACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGt                                                            <  2:335388/251‑1 (MQ=255)                                                                                                                                                                                         aaCCGTCGATAAATACGTCCGTCAGGGGTTTATGGCACAACAGAAGAGCTACCCCTACCTGCTCTGCCCACACTGACTGGCCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCCATTTAACGCATCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTc                                                         <  2:297970/251‑1 (MQ=255)                                                                                                                                                                                         aaCCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTc                                                         <  2:100502/251‑1 (MQ=255)                                                                                                                                                                                          aCCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCa                                                        <  1:50762/251‑1 (MQ=255)                                                                                                                                                                                               cGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGGGTATGGGCAGACTGGCGTTTCAGGtt                                                    <  1:201039/250‑1 (MQ=255)                                                                                                                                                                                                     aTACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGCTGCTTATCAAACAACGCACGCAGGGGCGTATGGGCAGACTGGCGTTTCCGGTTAAcccgt                                             >  2:242798/1‑248 (MQ=255)                                                                                                                                                                                                                   ggTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAACAACCGTTcccg                               >  2:358817/1‑248 (MQ=255)                                                                                                                                                                                                                       tATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCCAACAACGCCCGCAGTGGCGTATGGGCAGAATGGCGTTTCAGGTTAACCAGTAACAACAGTTCACGTTgg                           >  2:25931/1‑250 (MQ=255)                                                                                                                                                                                                                             acaacaGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAACAACAGTTCACGTGGTAAtgtg                     <  2:421341/251‑1 (MQ=255)                                                                                                                                                                                                                               aacaGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTTCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGGTAACCAGTAACAACAGTTCACGTTGTAATGTgcg                   >  1:512143/1‑251 (MQ=255)                                                                                                                                                                                                                                  agaataGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGGTTCAGGAGTTGCACGGCCTGACGTAACTGCTTCTGACTTAAGCGATTTAACTCCTCTCCCATCATGCCTCGGCGGt                                                                                                                       <  2:521998/148‑1 (MQ=255)                                                                                                                                                                                                                                  agaagaGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTATTTGAGGGTGAGTTACGTTCGTGTCAGGAGATGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGAATCGCCCATCATGCCCCGGCGGt                                                                                                                       >  1:521998/1‑148 (MQ=255)                                                                                                                                                                                                                                     agagATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGCGGGTGCGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAAAAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGGTAACCAGTAACAAAAGTTCACGTTGTAATGTGCGccaca              >  2:472176/1‑250 (MQ=255)                                                                                                                                                                                                                                     agagATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACGGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAACAACAGTTCACGTTGTAATGTGCGcaacaa             <  1:242798/251‑1 (MQ=255)                                                                                                                                                                                                                                      gagaTAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCCAACAACGCACGCCGGGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAACAACAGTTCACGTTGTAATGTGCGcaacaa             >  2:421756/1‑250 (MQ=255)                                                                                                                                                                                                                                                ttCCTGCTCCGCCCACACTGAATGACAGTAATATTGTTTGAGCGAGAGCACCGATCGTGTCAGGAGTTAAACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCACATCATGACCCGGCGGTTCTGCCCTACACGATGCTTATCAAACAACGCAAGCAGTTGCGTATGGGCAGAACGGCTTTTCCGGGTAACCAGTAACCACAGGTCACGCTGTAATGTGCGCAACAAAATAAACGGtt  >  2:500331/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       CAAGCATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCAGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAACAACAGTTCACGTTGTAATGTGCGCAACAAAATAACCGGTT  >  NC_000913/670392‑670881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑