| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 155,463 | A→T | *247K (TAA→AAA) | yadV ← | putative fimbrial chaperone YadV |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 155,463 | 0 | A | T | 96.5% | 105.1 / ‑6.1 | 29 | *247K (TAA→AAA) | yadV | putative fimbrial chaperone YadV |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (13/15); minor base C (1/0); total (14/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
ACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTACAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAAC > NC_000913/155230‑155699 | aCGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCACATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGGATTATATCCTTGCCTGATTTCAGCCTGGCAGTACCtt < 2:6951/251‑1 (MQ=255) cgtcgtAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTc > 2:131183/1‑251 (MQ=255) ccGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGccacc < 1:416256/251‑1 (MQ=255) cGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCAtt > 1:331979/1‑251 (MQ=255) cACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTaaaa < 1:400257/251‑1 (MQ=255) gagaTCAATATTAGATGCATTCTGCCCCATCAGGAAGGAATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTCGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAACTGCACTTTTGc > 2:163567/1‑250 (MQ=255) tAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCtt < 2:19109/250‑1 (MQ=255) tCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGAc > 1:225498/1‑251 (MQ=255) cAGGAAGGTATGTTCATATTCAACGAGTTCAGCACTGAAAGCAGTAGTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATTATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGTAATTATACCCCTGCCTGATTTCAGCCTGGCATTACCTTCAATTCCGCCACCAAAGTCATTAATGGCTTAAAGATGCGCATTTGCAGAATTCGCTTCGCCAGTAAGGCCTTTGAc < 2:351411/250‑1 (MQ=255) ggAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCttt > 1:492925/1‑230 (MQ=255) ggAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCAGTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCttt < 2:492925/230‑1 (MQ=255) atTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACt < 1:131183/251‑1 (MQ=255) aCGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATg < 2:331979/251‑1 (MQ=255) aGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATc < 2:320195/251‑1 (MQ=255) tATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAAt > 1:398943/1‑251 (MQ=255) gcgcAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGc < 2:3574/251‑1 (MQ=255) aaCGTGGCGATAAGGGAAAGCTGATGCTAATTTTTAGAATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTAAGCATGGCATTACCTTCAATTGCGCCACCCAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATAACTAAATGGTGCAATCATTTTCACATCACTCGGATAGCGTTTACCGCTAGCtt > 2:503722/1‑251 (MQ=255) aTACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATcac > 2:83425/1‑251 (MQ=255) tttttAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGcc < 1:128341/138‑1 (MQ=255) tttttAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGcc > 2:128341/1‑138 (MQ=255) atatTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGtt < 1:467151/251‑1 (MQ=255) tCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGAt > 2:274419/1‑251 (MQ=255) aCGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTCCAGCCTAGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTCAAACTGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTCAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAACTCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTc > 1:496587/1‑251 (MQ=255) aaTTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTAATCACTAAATGGGGAAATCATTTTCACATCAATCGGTTACCGTTTACCGCTAGCTTCTAATTCACCACTGCTAAAAGAGACGTGGTAAGGGGTTGGATTGGTCACTCGTACTGAGGCCTTACCtt > 2:386536/1‑251 (MQ=255) aTTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAACAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGAAGCCTTACCTTc > 2:409082/1‑251 (MQ=255) tataTCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGc < 1:136367/194‑1 (MQ=255) tataTCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGc > 2:136367/1‑194 (MQ=255) atCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGaa < 2:398943/250‑1 (MQ=255) atCCTTGCCGGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGGCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTGTCATGACCTCATCACTAAATGGTGCAATCATTTTCGCATCAAGCGGATAGCGTTTACCGCTAGCTTCTAAATTGCCACTGCTAAAAGAGACGTAGTAAGGGTTTGTATTGGGCACTTGTAATGCCCCCTGACCGTCTGAAc < 2:519894/251‑1 (MQ=255) | ACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTACAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAAC > NC_000913/155230‑155699 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |