| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,261,842 | 0 | T | G | 25.8% | 114.4 / 10.2 | 66 | T12P (ACC→CCC) | prs | ribose‑phosphate diphosphokinase |
| Reads supporting (aligned to +/- strand): ref base T (9/40); new base G (16/1); total (25/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.37e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAGTAGGGGATAACAGCGGTGATACGACCTGCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTGTATTAAAGAGCGC‑GATGCAACGTCTGGAACAAGGTGACGTTGTCACCGAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGCCTTTCGCCACAAAGCCATTGAGCCAT > NC_000913/1261601‑1262089 | aaGTAGGGGATAACAGCGGTGATACGACCTGCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTAcc < 1:1579/251‑1 (MQ=255)aaGTAGGGGATAACAGCGGTGATACGACCTGCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGGTGGCAATATGTTTTGCTAGTTCC‑GGGGGGGGGTTAcc > 2:374947/1‑251 (MQ=255) aGTAGGGGATAACAGCGGTGATACGACCTGCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGGGGCGTTAccc > 1:418278/1‑250 (MQ=255) gTAGGGGATAACAGCGGTGATACGACCTGCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGTCTTCGCCATCGCTAAAGCGACCTACAGCGGCTTCGCCGAGTGAAGTGTACAGGCGGTTGTCAATACGTTGTGCTAGTTCC‑GGGGGGGCGTTACCAg < 1:408721/251‑1 (MQ=255) gTAGGGGATAACAGCGGTGATACGACCTGCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCAGCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAg < 2:528639/251‑1 (MQ=255) gATAACAGCGGTGATACGACCTGCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCCATACGTTGTGCTAGTTTC‑GGGGTGGGGGTACCAGCaaaa > 1:942/1‑250 (MQ=255) cAGCGGTGATACGACCTGCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTc < 2:294971/251‑1 (MQ=255) gACCTGCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTCACCAGCAAAAAGCTTCATAT‑CAGGCACg < 1:2500/251‑1 (MQ=255) gCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGATAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATAAGTTGTGGTAGTTCC‑GGGGGGGGGTTACCAACAAAAAACTTCATAT‑CAGGcccggga > 2:333872/1‑243 (MQ=255) gCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATGCCATCAGGTTGTCGGTAGTAGGGGCACAAGTGGACTGGATGATGAAAAGATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGagaag < 2:49459/251‑1 (MQ=255) aaGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GTGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAAcc < 1:445078/251‑1 (MQ=255) gacgCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGc < 1:298356/251‑1 (MQ=255) gCATCAACCATAACGACTAATTCCATCAGTTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCCCCACCGCGTTCATTTTCATTAATTTGTACGCTGACTTCGCCATCGCGAAAGCGACCTACATCGGCGTCTCCGAGTGAAGTGTACAGGCGGTTGGCAATACTTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCGTCATAT‑CAGGCACGATAAAAACCTCAGGCATGCGTcc < 2:357127/251‑1 (MQ=255) aaCCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTACATtgg < 1:324464/251‑1 (MQ=255) aCCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATtggt < 1:444110/251‑1 (MQ=255) aaCGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCTCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGaaa < 1:293736/251‑1 (MQ=255) aCTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGGTGTGCTAGTTCC‑GGGGTGGGGTTACCAGCAAAAAACTTCATAT‑CCGGGACGGGAAGAAACTCCGGCATGCggccctttgggggaagaa > 1:416291/1‑233 (MQ=255) aaTTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGGCCATTGGTGGAAAGAATCt < 1:431693/250‑1 (MQ=255) cAGGTTGTAGTTAGTAGGGGCCAAAGTGGACTGGATGATGAAAATATCACCACCGCGTCCATTTTCATTAATTTGTACGCTGACTTAGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCGATACGTTGTGCTAGTTCCCGGGGGGGGGTT‑CCAGCAAAAAGCTTTCTAT‑CAGGAAAGAGAAGAACCACCCGCAGGCGTCCACTGGAGGAAAGaaattaccgcaaac > 2:486945/1‑239 (MQ=255) ggTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAACTATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTAGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGCGTGAAGTGTACAGGCGGTTGGGAATAAGTTGTGCTTGTTCC‑GGGGGGGGGTTACCCGCAAAAAGCTTTATAT‑CCGGCACGAGAAGAAACTCCGGCATGCGTCCCTTGGTGGGAAGAAACTGCCG‑AAAACtg > 1:508705/1‑251 (MQ=255) tGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCGCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACtgtg < 1:474018/250‑1 (MQ=255) tagtagGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACTGTGCGGGcc < 2:418278/250‑1 (MQ=255) tagtagGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACTGTGCGGGcc < 2:437558/250‑1 (MQ=255) cacaAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCATGATc < 2:942/251‑1 (MQ=255) acaAGTGGACTGGATGATGACAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTTC‑GGGGTGGCGGTACCAGCAAAAAGCTTCATAT‑CCGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAAACTGCCGGAAAAC‑GTGCGGGCCAGGCATGATcc > 1:164451/1‑251 (MQ=255) acaAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCATGATcc < 1:6402/251‑1 (MQ=255) caAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCATGATCCt < 2:143865/251‑1 (MQ=255) tGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑CGGGGGGCGTTACCAGCAAAAAGATTCATATACAGG‑ACGGGAGGAACCTCCCGCACGCGTCCATTGTATGTAAGAAACTGCCG‑AAAACACTGCGGGCCAAGCaagaaccctttc > 2:406944/1‑240 (MQ=255) ggACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTGACCAGCAAAAAGCTTCATAT‑CAGTCACGAGAAGAACCTCAGGCATGCGTCCAGTGGTGGAAAGAATCTGCCG‑AAAACTGGGCGGGCCAGGCATGATCCTTTCCa < 2:508705/251‑1 (MQ=255) ggATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCg < 2:85820/250‑1 (MQ=255) gaAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAgg < 1:348306/177‑1 (MQ=255) atatCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGGAATACGTTGTGCTAGTTCC‑GGGGGGGCGGTACCCGCAAAAAACTTCATAT‑CAGGCACGGGAAGAAACTCAGGCATGCGTCCATTGGGGGAAAGAATATGCCG‑AAAACTGTGCGGGCCAGGCATGTTCCTTTTCACGCGGTGTAtttaac > 1:173085/1‑246 (MQ=255) tatCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑CGGGGGGCGGTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACACCAGGCAGGCGTCCCTTGGTGGAAAAAATCTGCCG‑AAAACTGTGCGGGGCCGGCACGATCCTTTCCAGGCGGGGTGTTaaaa > 2:164149/1‑248 (MQ=255) caccacCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTTGGAATACGTTGTGCTAGTTCC‑GGGGGGGCGTTACCCGCAAAAAGCTTCATAT‑CAGGCACGGGAAGAACCCCAGGCATGCGGCCATTGGTGGGAAGAATCTTCCG‑GAAAATGTGCGGGCCAGGGAAGATCCTTTCCAGGAGGGGGATTaaaaagggc > 1:318989/1‑245 (MQ=255) ccGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTGTATTAAAGAGCGC‑GATGc < 1:418007/251‑1 (MQ=255) gTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGATCAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGGCATCCGTTGTGCTAGTTCC‑CGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGGACGAGAAGAACCTCAGGCATGCGTCCCTTGGGGGAAAGAATCTGCCG‑AAAACTGTGCGGGCCCGGCATGATTCTTTCCCGGCGGGGTATTAAAGAGCGC‑GATGCaa > 1:52617/1‑249 (MQ=255) cATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAG‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTGTATTAAAGAGCGC‑GATGCAACGTCt < 2:52617/251‑1 (MQ=255) tGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGCAGTGTACAGGCGGTTGGGAATACATTGTGCTAGATCC‑CGGGGGGCGGTACCCGCCCAAAGCTTCCAAA‑CAGGCACCAGAAGAACCTCCCGCATGCGTCCATTGGTGGAAAGAATATGCCG‑GAAACGGTGCGGGCCAGGCCTGCTCCCTTCCCGGCGGGGTATTTAAGAG‑GCAGATGCAACGTATGGGACaaaggaccg > 1:202487/1‑244 (MQ=255) gTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTGTATTAAAGAGCGC‑GATGCAACGTCTGGAACAAGGTGACGt < 1:86192/251‑1 (MQ=255) gTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGGGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCCTTTGTGGAAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGGGTATTAAAGAGCGC‑GATGCAACGTCTGGAAAAAGGTGACgg > 1:164024/1‑250 (MQ=255) gACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGGTTC‑GGGGGGGGGGTACCCGCAAAAAGATTCATAT‑CAGGCACGAGAAGAACCTCAGGCAAGCGTCCATTGGTGGGAAAAAACTGCCG‑AAAACTGTGCGGGCCAGGCAGGATCCTTTCCAGGCGGTGGATTAAAAAGCGC‑GCTGGCACGTCTGGAACAAGGTggcggtgttccc > 2:177088/1‑240 (MQ=255) aCTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGGGGCGTTACCCGCAAAAAGCTTCCTAT‑CAGGCACGAGAAGAACCTCCGGCATGCGTCCCTTGGGGGGAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCATGATCCTTTCCCGGCGGGGTATTAAAAAGGGC‑GATGCAACGTCTGGAAACAGGGGACGTTGTCAccc > 1:120150/1‑250 (MQ=255) ttCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAAAACGTTGTGCTTGTTCC‑GGGGTGGCGTTATCAGCCAAAAACTTCATAT‑CAGGGACCAGAAAAACCTCCGGCCTGCGTCCCTTGGTGGAAAGAATCTTCCGGAAAATTGT‑CGGGCCCGGCATGATCCTTTCCTGGCGGGGTATTTAAAAGCGC‑GATGCAAAGTCTGGAACaaagggaccttgttaccca > 1:114875/1‑233 (MQ=255) aTCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGGGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCCTTGGTGGGAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTGTATTAAAAAGCGC‑GGTGCAACGTCTGGAAAAAGGTGACGTTTTTACCGAAACTc > 1:388381/1‑249 (MQ=255) aTCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGGTGTGGTAGTTTC‑GGGGGGGCGTTACCAGCAAAAAGATTCATAT‑CAGGCAAGGGAAGAACCTCAGGCATGCGTCCATTGGTGGGAAGAATCTGCCG‑AAAACTGTGGGGGGCAGGGATGGTCCTTTTCAGGCGGGGGATTTAAGAGGGG‑GGGGCAACGTCTGGGACAAGGGGACGtttgcccccaaactccg > 1:45441/1‑236 (MQ=255) gCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTGTATTAAAGAGCGC‑GATGCAACGTCTGGAACAAGGTGACGTTGTCACCGAAACTCAGCtt < 1:224971/251‑1 (MQ=255) aaGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTGTATTAAAGAGCGC‑GATGCAACGTCTGGAACAAGGTGACGTTGTCACCGAAACTCAGCTTGccc < 1:164149/251‑1 (MQ=255) gcgTCGCCGAGTGAAGTGTACAGGCGGTTTGCAATACGTTGTGCTAGGTCC‑CGGGGGGCGTTACCAGAAAAAAGCCTCATAT‑CAGGCACGGGAAGAAACCCCGGCATGCGTCCATTGGGGGGAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCATGATCCTTTCCCGGCGGGGTATTAAAGAGGGC‑GGTGCAAAGTCTGGAACAAGGGGGCGTTGTCACCGCAACTCCGCTTGCCCGGCTTAAAGCCTggg > 1:464640/1‑250 (MQ=255) cGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTGTATTAAAGAGCGC‑GATGCAACGTCTGGAACAAGGTGACGTTGTCACCGAAACTCAGCTTGCCCGGCTTAAAGCATGGctct < 1:370511/250‑1 (MQ=255) ccGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCAGGATCCTTTCCAGGCGGTGTATTAAAGAGCGC‑GATGCAACGTCTGGAACAAGGTGACGTTGTCACCGAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCtgtg < 1:486945/251‑1 (MQ=255) caggttGAAGTGTACAGGCGATTGGCAATACGTTGTTCGGGGTCC‑GGGGTGGCGTTACAAGCAAATAGCTGCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACTGTGCGTGCCAGGCATGATCCTTTCCAGGCGGGGTATTAAAGAGCGC‑GATGCAACGTCTGGATCAAGGTGACGTTGACACCGAAACACAACTTGCCCGGATTACAGCATGGCTCtgtg < 2:419126/246‑1 (MQ=255) cGAGTGAAGGGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTGTATTAAAGAGCGC‑GATGCAACGTCTGGAACAAGGTGACGTTGTCACCGAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGc < 2:120150/251‑1 (MQ=255) gTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTGTATTAAAGAGCGC‑GATGCAACGTCTGGAACAAGGTGACGTTGTCACCGAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAAt < 1:488721/251‑1 (MQ=255) aaGTGTACAGGCGGTTGGCAATCCGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAG‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTGTATTAAAGAGCGC‑GATGCAACGTCTGGAACAAGGTGACGTTGTCACCGAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATggg < 2:18716/251‑1 (MQ=255) aGTGTACAGGCGGTTGGCAAGACGTTGTGCTAGGTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTGTATTAAATAGCGC‑GATGCAACGTCTGTAACAAGGTGACGTTGTCACCGAAACTGAGCTGGCCCGGGTTAAATCATGGCTCTGTACAATgggg < 2:491525/251‑1 (MQ=255) gTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTGTATTAAAGAGCGC‑GATGCAACGTCTGGAACAAGGTGACGTTGTCACCGAAAc < 1:19520/199‑1 (MQ=255) gTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTGTATTAAAGAGCGC‑GATGCAACGTCTGGAACAAGGTGACGGTGTCACCGAAAc > 2:19520/1‑199 (MQ=255) tGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTGTATTAAAGAGCGC‑GATGCAACGTCTGGAACAAGGTGACGTTGTCACCGAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGcc < 1:401051/250‑1 (MQ=255) tGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTGTATTAAAGAGCGC‑GATGCAACGTCTGGAACAAGGTGACGTTGTCACCGAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGcc < 1:333872/250‑1 (MQ=255) gCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTGTATTAAAGAGCGC‑GATGCCACGTCTGGAACAAGGTGACGTTGTCACCGAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGCCttt > 2:378880/1‑251 (MQ=255) cAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTGTATTAAAGAGCGC‑GATGCAACGTCTGGAACAAGGTGACGTTGTCCCCGAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGCCTTTc < 1:116499/251‑1 (MQ=255) aaTACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTGTATTAAAGAGCGC‑GATGCAACGTCTGGAACAAGGTGACGTTGTCACCGAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGCCTTTCg > 2:415153/1‑251 (MQ=255) aaTACGTTGTGCTAGTGCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTGTATTAAAGAGCGC‑GATGCAACGTCTGGAACAAGGTGACGTTGTCACCGAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGCCTTTCg < 2:164451/251‑1 (MQ=255) cGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTGTATTAAAGAGCGC‑GATGCAACGTCTGGAACAAGGTGACGTTGTCACCGAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGCCTTTCGCcac > 1:92445/1‑251 (MQ=255) cc‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTGTATTAAAGAGCGC‑GATGCAACGTCTGGAACAAGGTGACGTTGTCACCGAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGCCTTTCGCCACAAAGCCATTGAg < 1:453679/250‑1 (MQ=255) gggTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTGTATTAAAGAGCGC‑GATGCAACGTCTGGAACAAGGTGACGTTGTCACCGAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGCCTTTCGCCACAAAGCCATTGAGCCAt < 1:175522/251‑1 (MQ=255) | AAGTAGGGGATAACAGCGGTGATACGACCTGCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCC‑GGGGTGGCGTTACCAGCAAAAAGCTTCATAT‑CAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG‑AAAACTGTGCGGGCCAGGCATGATCCTTTCCAGGCGGTGTATTAAAGAGCGC‑GATGCAACGTCTGGAACAAGGTGACGTTGTCACCGAAACTCAGCTTGCCCGGCTTAAAGCATGGCTCTGTGCAATGGGGAAAGATTAGCGCCTTTCGCCACAAAGCCATTGAGCCAT > NC_000913/1261601‑1262089 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |