Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	119,396	0	C	T	28.6%	61.8 / 10.6	43	S39F (TCT→TTT)	ampE	protein AmpE
Reads supporting (aligned to +/- strand):  ref base C (8/22);  new base T (12/0);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 1.14e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TG‑AAACATTTTTCTCTCGGGCGCAC‑G‑TTAGGCATGACCATTATTGCGATGGGCGTGA‑CTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCC‑ACGCTACTGGTGTGGCTGCTG‑ATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTT‑CACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCG  >  NC_000913/119170‑119640                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                           tatCGCTAAAAACATGACGGGCCATTGTGCTATTGCACCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTACTTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑GGGAAACATTTTT‑TTTCTGGCGCAC‑G‑tatggggtgtc                                                                                                                                                                                                                                  >  2:521484/1‑241 (MQ=255)                                     gcCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TG‑AAACATTTTTCTCTCGGGCGCAC‑G‑TTAGGCATGACCATTATTg                                                                                                                                                                                                                          <  2:414029/223‑1 (MQ=255)                                     gcCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑GG‑AAACATTTTTCTCTCGGGCGCAC‑G‑TTAGGCATGACCATTATTg                                                                                                                                                                                                                          >  1:414029/1‑223 (MQ=255)                                       cGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑GG‑AAACATTTTTCTCCCGGGCGCACCG‑TT‑GGCAAGGCCCTTATTGCGGTGGGCGGGG‑Ctttttttttgttaagcg                                                                                                                                                                                           >  1:270364/1‑240 (MQ=255)                                         gATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGTG‑AA‑CATTTTTTTTTCTGGCGCAC‑G‑TTATGCATGACCATTATTGCGGTGGGGGTGA‑Ccttttttctttttttccgc                                                                                                                                                                                         >  2:78383/1‑238 (MQ=255)                                             ggAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑GG‑GAAAATTTTTTTTTCCGGGGCAC‑C‑TTTGGCCAGTCCCTTATTTCGGTGGGCGTGA‑Cttttttttcgttacccgcatttc                                                                                                                                                                                     >  2:240623/1‑234 (MQ=255)                                              gAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTTCAACCTTACTGGAGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGACGCGTTCTTTCGGCGGC‑AG‑AAACCTTTTTTTCTCCGGCGCGC‑GATGAGGAAGGGCC‑TTTTTGCGATAGGCGTGG‑CTTTTTTACTGTTAggcggcctgc                                                                                                                                                                                     >  1:325744/1‑244 (MQ=255)                                                aaaCCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGATACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCTCTGTCAGCGTGAGCAGCGGCTTGAAGGGGTCTATCGGCGTT‑TG‑AAACATTGTGCTCGCTGGCGCAC‑G‑TTAGCCAGGGCCATTACTGGTATGGGCGTGA‑CTTTTTTACTGTTACGCGCATTGCAgg                                                                                                                                                                                  <  1:245633/251‑1 (MQ=255)                                                  aCCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑GG‑GAACATTTTTCTTTCGGGCGCAC‑G‑TCAGGGATGACCCTTATTTGGATGGGCGTGA‑TTTTTTTCCTGTTACCCGCCTTGCCgggg                                                                                                                                                                                >  1:405498/1‑250 (MQ=255)                                                    cGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTACTTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑CG‑AAAAATTTTTCTCTTCGGCGCAC‑G‑TTAGGCATGACCATTATTGCGATGGGGGTGA‑CTTTTTTTCTGTTACGCGCATTGCCGGGAgg                                                                                                                                                                              >  1:193080/1‑250 (MQ=255)                                                     gATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TG‑AAACATTTTTCTCTCGGGCGCAC‑G‑TTAGGCATGACCATTATTGCGATGGGCGTGA‑CTTTTTTACTGTTACGCGCATTGCAGGGAGTa                                                                                                                                                                             <  1:201710/251‑1 (MQ=255)                                                           ggTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TG‑AAACATTTTTCTCTCGGGCGCAC‑G‑TTAGGCATGACCATTATTGCGATGGGCGTGA‑CTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTc                                                                                                                                                                       <  1:153352/251‑1 (MQ=255)                                                                ggCATTTGATTGGGCTCGGTTTCGTGTTCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTGAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCTGCGGG‑TG‑AATCATTTTTCTCTCGGGCGCAG‑G‑TTAGGCATGACCATTTGTGCGAGGGGCGTGA‑CATTTTTACTGTTACGCGCATGGCAGGGAGTATTGTGCAACGt                                                                                                                                                                  <  2:467977/250‑1 (MQ=255)                                                                 gCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TG‑AAACATTTTTCTCTCGGGCGCAC‑G‑TTAGGCATGACCATTATTGCGATGGGCGTGA‑CTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGt                                                                                                                                                                  <  1:52495/250‑1 (MQ=255)                                                                   aTTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑GG‑AAACATTTTTTTCTCGGGCGCAC‑G‑TTAGGCCTGACCATTATTTCGATGGGGGTGG‑CTTTTTTTCTGTTTCGCGCCTTGCAGGGAGTGTTGTTTAActtttc                                                                                                                                                               >  2:134639/1‑246 (MQ=255)                                                                   aTTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑GG‑AAACATTTTTTTCTCGGGCGCAC‑G‑GTAGGCATGACCATTATTGCGATGGGGGGGG‑CTTTTTTTCTGTTACGCGCATTGCAGGGGGTATTGTTTAACGtttc                                                                                                                                                               >  1:493616/1‑249 (MQ=255)                                                                   aTTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGAAAACATGACGCTATTTACAACCTTACGGGTGTTAATGTTCGAGCGCCTGGTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TG‑AAACATTTTTCTCTCGGGCGCAC‑G‑TTAGGCATGACCATTATTGCGATGGGCGTGA‑CTTTTTTACTGTTACGCGCATTGCAGGGAGCATGTTTCAACGTTcc                                                                                                                                                               <  2:433118/251‑1 (MQ=255)                                                                       gATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TG‑AAACATTTTTCTCTCGGGCGCAC‑G‑TTAGGCATGACCATTATTGCGATGGGCGTGA‑CTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCC‑Ac                                                                                                                                                           <  1:373196/250‑1 (MQ=255)                                                                             gCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑GG‑AAAAATTTTTCTCTCGGGCGCAC‑G‑TTTGGCATGACCCTTATTGCGATGGGGGTGA‑CTTTTTTTCTGTTACGCGCATTTCAGGGGGTATTTTTCAACGTTCCC‑ACGCTACTg                                                                                                                                                    >  1:236784/1‑251 (MQ=255)                                                                                      cGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TG‑AAACATTTTTCTCTCGGGCGCAC‑G‑TTAGGCATGACCATTATTGCGATGGGCGTGA‑CTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCC‑ACGCTACTGGTGTGgct                                                                                                                                            <  1:113040/250‑1 (MQ=255)                                                                                       gtgtGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑GG‑GAACATTTTTTTTTCTGGCGCAC‑G‑TTAGGCATGACCCTTATTTCGATGGGGGTGG‑CTTTTTTTTTGTTTTGCGCCTTGCCGGGAGGATTGTTCAAAGTTTCC‑CCCCTACTTGTGTGgctgg                                                                                                                                          >  2:5539/1‑250 (MQ=255)                                                                                         ttgCTGGTCAGCAAGGAGCCAACATGACGCTATTTACAGCCTTACTGGTGTTAAGGTTCGAGCGCCGGTTTAAGGTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TG‑AAACATTTTTCTCGCGGGCGCAC‑G‑TTAGGCATGACCATTATTGCGATGGGCGTGA‑CTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCC‑ACGCTACTGGTGTGgctgct                                                                                                                                         <  2:399467/249‑1 (MQ=255)                                                                                                         agaCAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTGCGGCGGG‑TG‑AAACATTTTTCTCTCGGGCGCAC‑G‑TTAGGCAt                                                                                                                                                                                                                                     <  1:91748/144‑1 (MQ=255)                                                                                                            caacaTGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TG‑AAACATTTTTCTCTCGGGCGCAC‑G‑TTAGGCATGACCATTATTGCGATGGGCGTGA‑CTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCC‑ACGCTACTGGTGTGGCTGCTG‑ATTGGTTTGCTGTGTATTg                                                                                                                    <  1:521484/251‑1 (MQ=255)                                                                                                            caacaTGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑GG‑AAACATTTTTTTCTCGGGCGCAC‑C‑TTAGGCATGACCCTTATTGCGATGGGCGGGA‑CTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCC‑ACGCTACTGGTGTGGCTGCTG‑ATTGGTTTGCTGTGTAttt                                                                                                                    >  1:403335/1‑250 (MQ=255)                                                                                                              acaTGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TG‑AAACATTTTTCTCTCGGGCGCCC‑G‑TTAGGCATGACCATTATTTCGATGGGCGTGA‑CTTTTTTTCTGTTACGCGCCTTGCAGGGGGGATTGTTCAACGTTCCC‑ACGCTACTGGTGGGGCTGCTG‑ATTGGTTTGCTGTGTATTggg                                                                                                                  >  1:162675/1‑250 (MQ=255)                                                                                                              acaTGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TG‑AAACATTTTTCTCTCGGGCGCAC‑G‑TTAGGCATGACCATTATTGCGATGGGCGTGA‑CTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCC‑ACGCTACTGGTGTGGCTGCTG‑ATTGGTTTGCTGTGTATTGgc                                                                                                                  <  1:404985/251‑1 (MQ=255)                                                                                                                aTGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TG‑AAACATTTTTTTCCCTGGCGCCC‑G‑TTAGGCATGACCCTTTTTTCGATGGGCGTGA‑CTTTTTTTCTGTTACGCGCATTTCAGGGGGGATTGTTTAACGTTTCC‑ACGCTACTGTGGTGGCTGCTG‑GTTTGTTTTCtgggttatgtggc                                                                                                                >  2:167009/1‑240 (MQ=255)                                                                                                                         tttACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGACGCGTTCTTTCGGCGGG‑GG‑AAACATTTTTTTTTCTGGCGCAC‑G‑TTTAGCATGGACACTATTGGGGTGGGCGTGAACTTTTTT‑CTTTTATGCGCATTGCTGGGGGGGTTGTTCCACGTTCCCCAC‑CCACTTGTGTGGGTGCTG‑ATTGTTTTGCTGTGTATTgggggcgggacagg                                                                                                       >  2:480987/1‑239 (MQ=255)                                                                                                                           tACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCACCGTCTTGAAGCGTTCTTTCGGCGGG‑TT‑AAACATTTTTCTCTCGGGCGCAC‑G‑TTAGGCATGACTATTATTGCGATGGGCGTGT‑GTGTGTGACTGTTACGCGCATTTGAGTTAGTATTGTTCAACGTTCCC‑ACGCTTCTGGTGGGGCTGCTG‑ATTGGTGTGCTGTGTATTGGCGCAGGTAAAGtt                                                                                                      <  1:240244/250‑1 (MQ=255)                                                                                                                            aCAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TG‑AAACATTTTTCTCTCGGGCGCAC‑G‑TTAGGCATGACCATTATTGCGATGGGCGTGA‑CTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCC‑ACGCTACTGGTGTGGCTGCTG‑ATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCg                                                                                                    <  1:225328/251‑1 (MQ=255)                                                                                                                               aCCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TG‑AAACATTTTTCGCTCGGGCGCAC‑G‑TTAGGCATGACCATTAGTGCGATGGGCGTGA‑CTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTGCAACGTTCCC‑ACGCTACTGGTGTGGCTGCTG‑ATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCt                                                                                                 <  1:518921/251‑1 (MQ=255)                                                                                                                                    aCTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TG‑AAACATTTTTCTCTCGGGCGCAC‑G‑TTAGGCATGACCATTATTGCGATGGGCGTGA‑CTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCC‑ACGCTACTGGTGTGGCTGCTG‑ATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCAt                                                                                             <  1:5539/250‑1 (MQ=255)                                                                                                                                         ggtTAATTTTCGAGCGCCTGTTGAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TG‑AAACATTTTTCTCTCGGGCGCAC‑G‑TTAGGCATGACCATTATTGCGATGGGCGTGA‑CTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCC‑ACGCTACTGGTGTGGCTGCTG‑ATTGGGTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCAt                                                                                       <  1:80894/250‑1 (MQ=255)                                                                                                                                             aaTTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TG‑AAACATTTTTCTCTCGGGCGCAC‑G‑TTAGGCATGACCATTATTGCGATGGGCGTGA‑CTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCC‑ACGCTACTGGTGTGGCTGCTG‑ATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCt                                                                                    <  1:327977/250‑1 (MQ=255)                                                                                                                                             aaTTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑CG‑AAACATTTTTTTCTTGGGCGGCC‑G‑TTAGGGCTGAACATTATTGCGATGGGCGGGA‑CTTTTTTACTGTTACGCGCATTGCAGGGGGTATTGTTCAACGTTCCC‑ACGCTACTGGTGTGGCTGCTG‑GTTGGTTTGCTGTGTATTGGCGCAGGGAAAGTTCGTCTTCCTTATCATGCtt                                                                                   >  1:498395/1‑251 (MQ=255)                                                                                                                                                            gTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TG‑AAACATTTTTCTCTCGGGCGCAC‑G‑TTAGGCATGACCATTATTGCGATGGGCGTGA‑CTTTTTTACTGTTACGCGCATGGCAGGGAGTATTGTTCAACGTTCCC‑ACGCTACTGGTGTGGCTGCTG‑ATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCtt                                                                    <  2:493616/251‑1 (MQ=255)                                                                                                                                                                         gAGCACTAGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑CG‑AAACATTTTTTTCTCGGGCGCAC‑G‑ATAGGCCTGACCATTTTTGCGATGGGGGTGA‑CTTTTTTTCTTTTACGCGCCTTGCCGGGGGTATTGTTCAACGTTCCC‑CCGCTACTGGTGTGGCTGCTG‑GTTGGTTTGCTGTGTATTGTCGCAGGGAAAGTTCGTCTTCCTTTTCATTCTTTTCTGACAGCTGCTT‑TACGTAAtaaaa                                                       >  2:239988/1‑246 (MQ=255)                                                                                                                                                                               tGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TG‑AAAAATTTTTTTCTCCGGCGCAC‑G‑GTTGGCATGTCCATTTTTGCGGTGGGCGTGA‑CTTTTTTTCTTTTACGCGCATTGGCGGGAGGATTGTTTAAAGTTCCC‑ACGCTACTGGTGTGGGTGCTGGTTTGGTTTG‑TGTGTTTTTGCGCCGGGAAAGTTCGTTGTCCATATTATGCTTATCTGCCAGACGCTTTCA‑GTAATGATAGcccttg                                                >  2:396367/1‑247 (MQ=255)                                                                                                                                                                                                                         g‑AAACATTTTTCTCTCGGGCGCAC‑G‑TTAGGCATGACCATTATTGCGATGGGCGTGA‑CGTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCC‑ACGCTACTGGTGTGGCTGCTG‑ATTGGTTTGCTGTGTATTGGCGCAGGTAAAGGTCGTCTTCATTATCATGCTTATCTGACAGCTTCTT‑CACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGAGTCACGGc        <  2:193080/250‑1 (MQ=255)                                                                                                                                                                                                                           aaaCATTTTTCTCTCGGGCGCAC‑G‑TTAGGCATGACCATTATTGCGATGGGCGTGA‑CTTTCTTCCTTTCACGCGCATTGCAGGGACTATTGTTCAACGTTCCC‑ACGCTACTGGTGTGGCTGCTG‑ATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTT‑CACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGACTTCACCATGATTCACGGCGt      >  2:410542/1‑251 (MQ=255)                                                                                                                                                                                                                             aCATTTTTCTCTCGGGCGCAC‑G‑TTAGGCATGACCATTATTGCGATGGGCGTGA‑CTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCC‑ACGCTACTGGTGTGGCTGCTG‑ATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTT‑CACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTcc    <  1:134639/251‑1 (MQ=255)                                                                                                                                                                                                                               aTTTTTCTCTCGGGCGCAC‑G‑TTAGGCATGACCATTATTGCGATGGGCGTGA‑CTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCC‑ACGCTACTGGTGTGGCTGCTG‑ATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTT‑CACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCg  >  1:379054/1‑251 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                           TATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TG‑AAACATTTTTCTCTCGGGCGCAC‑G‑TTAGGCATGACCATTATTGCGATGGGCGTGA‑CTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCC‑ACGCTACTGGTGTGGCTGCTG‑ATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTT‑CACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCG  >  NC_000913/119170‑119640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑