Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913115,7970CA22.7% 106.9 / 12.7 67E435* (GAA→TAA) hofBT2SSE family protein
Reads supporting (aligned to +/- strand):  ref base C (39/12);  new base A (0/15);  total (39/28)
Fisher's exact test for biased strand distribution p-value = 6.48e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCATCCCATCTTGCGCATT‑GCC‑GTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCAAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGGCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGC  >  NC_000913/115566‑116045
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                           
cgcAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCATCCCATCTTGCGCATT‑GCC‑GTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCAAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATAccc                                                                                                                                                                                                                                       <  1:83652/251‑1 (MQ=255)
 gcAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCGGCCCATAGCATCCCATCTTGCGCATT‑GCC‑GTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCAAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATAcccc                                                                                                                                                                                                                                      >  2:273720/1‑250 (MQ=255)
   aGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGTTACCCATCTGTTGCTGTAGTGCCATAATCAGTAAAGTACGGCTCTCTGCCCATAGCATCCCATCTTGCGCATT‑GCC‑GTCGCCGGTTATGCCATGCCAGCGCCAGAGTTTCTTACTCGCCAGGCG‑GCTTCCCC‑AGTACGCGGATTAACTCTTCAAAGG‑TGG‑TT‑AA‑GCCTTCCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CTTACGC‑AGACCCGcc                                                                                                                                                                                                                                    <  2:73038/251‑1 (MQ=255)
   aGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCATCCCATCTTGCGCATT‑GCC‑GTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCAAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGcc                                                                                                                                                                                                                                    >  1:237365/1‑251 (MQ=255)
         gATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCATCCCATCTTGCGCATT‑GCC‑GTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCAAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTcgt                                                                                                                                                                                                                              >  1:437047/1‑251 (MQ=255)
                  tCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCATCCCATCTTGCGCATT‑GCC‑GTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCAAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGtt                                                                                                                                                                                                                     >  2:152799/1‑251 (MQ=255)
                   cGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCATCCCATCTTGCGCATT‑GCC‑GTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCAAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGttt                                                                                                                                                                                                                    >  1:202895/1‑251 (MQ=255)
                     cTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCATCCCATCTTGCGCATT‑GCC‑GTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCAAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTcc                                                                                                                                                                                                                  >  2:415291/1‑251 (MQ=255)
                        cagtcTTAGTGGGGTAACCTTCTGTTGCTGTAGTGCCATACGCAGTAACGTACGGCTCTCTGCCCATAGCATCCCATCTTTCGCATT‑GCC‑GTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCCAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑CTACCCGCCTGTCGTGCGTGCGTGTCCAGc                                                                                                                                                                                                               >  2:95327/5‑251 (MQ=255)
                        cAGGTTTAGTGGGGTAACCATCTGTTGCTGTAGTCCCATAAGCAGTAAAGTCCGGCTCTCTGCCCATAGCATCCCATCTTGCGCATT‑GCC‑GTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTCCCCGCCATGGG‑GCACCCCC‑AGTACGCGGATTAACTCTTAAAAGG‑TGG‑TT‑AA‑CCCTTGCTCTACGGCCGGGCAGCCT‑‑TTTTCAAAAA‑G‑CGTCCGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGc                                                                                                                                                                                                               <  1:157318/251‑1 (MQ=255)
                        cAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCATCCCATCTTGCGCATT‑GCC‑GTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCAAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑CTACCCGCCTGTCGTGCGTGCGTTTCCAGc                                                                                                                                                                                                               >  2:237401/1‑251 (MQ=255)
                           gttgaGTGGGGTAACCTTCTGTTGTTGTAGGGCAATAAGAAGAAAAGTCCGGCTCTCTGCCCATAGCACCCCATCTTGCGCGTG‑GCC‑GTCGCCGGTTATGCCAGGCCAGCGCCAGATTTGCTTCCCCGCCATGCG‑GCACCCCC‑AGTACGCGGATTACCTCTTAAAAGG‑TGT‑TT‑AA‑GCTTTGCTCTCCGGCCAGGCAGCTG‑‑TTTTAAAAAG‑G‑CGTCCGC‑ATCCCCGCCTGTCGTGCGTGCGTTTCCAGCGAt                                                                                                                                                                                                            <  1:358132/247‑1 (MQ=255)
                                  ggggTAACCATCTGTTGCTGTAGTGCCATACGCAGTAAAGTACGGCTCTATGCCAATAGCATCCCATCTTGCGCATT‑GCC‑GTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCAAAGG‑GGG‑TT‑AA‑GCCTTGCTCTACGGCCAGTCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGGGGGTGCGTTTCCAGCGAttaaccgt                                                                                                                                                                                                     >  2:465339/1‑245 (MQ=255)
                                  ggggTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCATCCCATCTTGCGCATT‑GCC‑GTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCAAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGt                                                                                                                                                                                                     >  1:134583/1‑251 (MQ=255)
                                   gggTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCATCCCATCTTGCGCATT‑GCC‑GTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCAAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCGGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTc                                                                                                                                                                                                    >  1:138501/1‑251 (MQ=255)
                                    ggTAACCATCTGTTGCTGAAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCATCCCATCTTGCGCATT‑GCC‑GTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCAATGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCAAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAACA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGCTTCAACGTCg                                                                                                                                                                                                   >  2:483622/1‑251 (MQ=255)
                                         ccATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCATCCCATCTTGCCCATT‑GCC‑GTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCAAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTAtt                                                                                                                                                                                              >  1:63753/1‑251 (MQ=255)
                                               gtggtgggaGTGCCAAAAGCAGAAAAGTCCGTCCCCCTCCCCATAGCTCCCCATTTTGCGCGTG‑GCC‑GTCGCGGTTTAGCCCGTGCCACCGCAAGGGTTGCTAACCCGCCATGCG‑CCACCCCA‑AGTACGCGAATAAACT‑TTAAAAGGGTGG‑TA‑AA‑GCTTTGCTCTACGGCCGGGCGCCCG‑‑TTTTAAAAAG‑G‑CGCGCGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGa                                                                                                                                                                                        <  2:105471/243‑1 (MQ=255)
                                                ttGCTGTAGTGCCATACGCAGTAAAGTACGGCTCTCTGCCCATAGCATCCCATCTTGCGCATT‑GCC‑GTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCAAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGa                                                                                                                                                                                        >  1:441978/1‑250 (MQ=255)
                                                  gCTGTAGCGCCAAAAGCAGAAAAGTACGGCTCTCTGCCCATACCACCCCCTCTTGCGCATG‑CCC‑GTCGCCGGTTATGCCAGGCCGGCCCCGGAGTTGCTTTCTCGCCATGCG‑GCACCCCC‑AGGCCGGGGATTACCTGTTCAAGGG‑TGG‑TT‑AG‑GCCTTGCTCTGGGGCGAGGCAGCCT‑‑TGTGAAAAAA‑G‑CTTACGC‑ATACCCGCCTGGCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAAt                                                                                                                                                                                     <  1:60080/251‑1 (MQ=255)
                                                        gTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCATCCCATCTTGCGCATT‑GCC‑GTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCAAAGG‑GGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATagtatg                                                                                                                                                                               >  2:282798/1‑246 (MQ=255)
                                                            cATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCATCCCATCTTGCGCATT‑GCC‑GTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCAAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACg                                                                                                                                                                            >  1:317987/1‑250 (MQ=255)
                                                             aTAAGCAGTAAAGGCCGCCTCCCTGCCCATGCCACCCCCTCTT‑CCCCTT‑GCCCGTCGCCGTTTATGCCTGGCCACCGCAAGGTTTGCTTCCTCCCCATGCG‑GCATCCCC‑AGTACGCGTATTACCTCT‑AAAAGG‑GGG‑TT‑AAAGCTTTCCTCTCCGCCCGGCCAGC‑G‑‑TTTTAAAAAAAG‑CGTACGA‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGaa                                                                                                                                                                          <  1:315745/251‑1 (MQ=255)
                                                                   aGTAACGTACGGCTCTCTGCCCATAGCATCCCATCTTGCGCATT‑GCC‑GTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCAAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTCGCGGAAATAAGCTGACGAATGACCg                                                                                                                                                                    >  2:462790/1‑251 (MQ=255)
                                                                   aGTAAAGTACGGCTCTCTGCCCATAGCATCCCATCTTGCGCATT‑GCC‑GTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCAAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCg                                                                                                                                                                    >  2:102753/1‑251 (MQ=255)
                                                                       aaGTACGCCTCCCTCCCCATAGCACCCCATCTTGCGCATG‑CCC‑GTCGCGGTTTATGCCATGCCAGCGCCAGAGTTGCTTACCCGCCAGGCG‑GCACCCCC‑AGTGCGCGGATAAACTCTTCAAAGG‑TGG‑TT‑AA‑GCTTTGCTCTACGGCCGGGCAGCCG‑‑TTTTCAAAAA‑G‑CGT‑CGCCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGt                                                                                                                                                                <  1:328833/251‑1 (MQ=255)
                                                                                  tctGCCCATAGCATCCCATCTTGCGCATT‑GCC‑GTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCAAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGa                                                                                                                                                      >  1:26518/1‑250 (MQ=255)
                                                                                     gCCCATAGCACCCCATCTTGCGCATT‑GCC‑GTCGCCGGTTATGCCACGCCAGCGCCAGAGTTGCTTCCTCGCCATGCG‑GCACCCCC‑AGGACGGGGATTAACTCTTAAAGGG‑GGG‑TT‑AA‑GCCTTGCTCTGCGGCCAGGCAGCCG‑‑TTTTAAAAAA‑G‑CGTGCGC‑ATCCCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAAc                                                                                                                                                    <  1:438581/249‑1 (MQ=255)
                                                                                             ccccccATCTTGCG‑ATTCGCC‑GCCCCCGTTTATCCCCTGCCAGCGCCAGATTTGCTTACTCCCCAGGCG‑GCCCCCCC‑AGTACGCGGATTAACTCTTAAAAGG‑TGG‑TT‑AA‑CCCTTGCTCTCCGGCGAGGCGGCCG‑‑TTTTCAAAAG‑G‑CGTCCCC‑ATCCCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAg                                                                                                                                          <  2:26518/248‑1 (MQ=255)
                                                                                             caccccATCTTGCGCATT‑GCC‑GTCGCCGTTTATGCCAGGCCAGCGCCAGAGTTGCTTACTCGCCATGCG‑GCACCCCC‑AGTACGCGGATTACCTCTTCAAAGG‑TGG‑TT‑AA‑GCTTTGCTCTACGGCCAGGCAGCCT‑‑TTTTAAAAAA‑G‑GGTCCGA‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAg                                                                                                                                          <  1:243944/248‑1 (MQ=255)
                                                                                                cccactttGCGCTTT‑GCC‑GTCGCCGGTTAGCCCATGCCAGCGCCAGAGTTGCTTACCCGCCAGGCG‑GCATCCCC‑AGTCCGCGGATTAACTCTTCAAGGG‑TGG‑TT‑AA‑GCTTGGCTCTACGGCCAGGCGGCCT‑‑TTTTAAAAAA‑G‑CGTACGC‑ATCCCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGcc                                                                                                                                       <  1:17195/245‑1 (MQ=255)
                                                                                                cccACCTTGCGCATT‑GCC‑GTCGCCGGTTAGGCCATGCCAGCGCAAGAGTTTTTTACTCGCCATGCG‑G‑ATCCCCCAGAACGCGAATAACCTCTAAAAGGG‑GGG‑GT‑AA‑CCCTTGCTCTACG‑CCAGG‑‑GCCGCTTTTTCAAAAA‑GTCTTACGC‑AGACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGcc                                                                                                                                       <  1:483622/251‑1 (MQ=255)
                                                                                                 ccATCTTGCGAATT‑GCC‑GTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCAATGCG‑GCATCCCC‑AGTACGCGGATTCAATCTTCAAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGAACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCACCGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCg                                                                                                                                      >  2:349906/1‑251 (MQ=255)
                                                                                                  cATCTTGCGCATT‑GCC‑GTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCG‑GCATCCCC‑AGTACGAGGATTAACTCTTCAAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCgg                                                                                                                                     >  2:192763/1‑250 (MQ=255)
                                                                                                         cgcATT‑GCC‑GTCGCCGGTTATGCAATGCCAGCGCCAGAGTTGCTTACTCGCCATGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCAAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGAcc                                                                                                                               >  2:363685/1‑250 (MQ=255)
                                                                                                           cATT‑GCC‑GTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCAACGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCAt                                                                                                                             >  1:253178/1‑250 (MQ=255)
                                                                                                                    gTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCCTGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCAAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCg                                                                                                                     >  1:475264/1‑251 (MQ=255)
                                                                                                                    gTCGCCGGTTATGCCATCCCAGCGCCAGAGTTCCTAACCCCCAATGCG‑GCACCCCC‑AGTACGCGGATTACCTCTTCAAAGT‑TGT‑TT‑AC‑GCTTTCCTCTCCGTCGAGGCAGCTT‑‑TTTTCAAAAA‑C‑CTTACCC‑ATTCCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCg                                                                                                                     <  1:303516/251‑1 (MQ=255)
                                                                                                                    gTCGCAGGGTATGCCACGCCAGCGCCAGAGTTGCTTACTCGCCATGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCAAAGG‑AGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCCGCCG‑‑TTTTCAAAAA‑G‑ACTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGGATTCGCGGAAATACACTGACGCCTGAACGGCGTTATGGGCCGAACCTCAAGTAATGCCGTACGCCCATacacaccc                                                                                                                     >  2:30243/1‑244 (MQ=255)
                                                                                                                          ggTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCAAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGt                                                                                                                                     >  2:102756/1‑229 (MQ=255)
                                                                                                                            taacgccatcccgGCGCCAGGGTTCCTTCCTCGCCGTGGG‑GCCCCCCC‑GGTCCGCGGATTACCCTTTCAAGGG‑TGA‑TT‑AA‑GCCTGCCTCAAGGGCCGGGC‑GGCG‑‑TTTTTAAAAA‑GGCGTACGC‑ATCCCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACGTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGc                                                                                                              <  1:461974/246‑1 (MQ=255)
                                                                                                                                gccatgccaGCGCCAGAGTTGCTTACTCGCCATGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCAAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTCCGACCATAAAAACCGTGGTAGCAATGt                                                                                                         >  2:301685/1‑251 (MQ=255)
                                                                                                                                 ccatgccaGCGCCAGAGTTGCTTACTCGCCATGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCCAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGt                                                                                                         >  1:463611/1‑250 (MQ=255)
                                                                                                                                        aGCGCCAGAGTTGCTTACTCGCCATGCG‑GCACCCCC‑ATTCCGCGGATTAACTCTTAAAGGG‑TGG‑TT‑AG‑GCCTTGCTCTACGGCGAGGCGGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAAcc                                                                                                 <  1:314448/251‑1 (MQ=255)
                                                                                                                                             cAGAGTTGCTTACTCGCCATGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCAAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑CTACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGtg                                                                                            >  2:80746/1‑251 (MQ=255)
                                                                                                                                               gagTTGCTTAATCGCCACGCGAGC‑TCCCC‑AGCAAGCGGCTTAAATCCACACAGG‑CGG‑TA‑AA‑GCATTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTAAGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTAGGAATTAGCGGAACTACGCTGACGAATGAACGGCGTTCTGGGCAGAACTTAAAATAAGGCCGTAAGACAATAAAAACCGTGGTAGCAATGTACACAACAGGGtgcc                                                                                          >  2:227381/1‑251 (MQ=255)
                                                                                                                                                    gCTTACCCCCCATGCG‑CCACCCCC‑AGGACGCGGATTACCCATACAAAGG‑‑GG‑TTGAC‑GCTTTGCTCCACGGCCGGGCAGCCG‑‑TTTAAAAAAA‑G‑CGTACAC‑ATCCCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAAGGTACACAACCGGGtgcctgcc                                                                                      <  2:237365/250‑1 (MQ=255)
                                                                                                                                                        aCTCGCCATGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCAAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTgg                                                                                 >  2:40032/1‑251 (MQ=255)
                                                                                                                                                               aTGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCAAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCCAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCACCGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGCACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGCGGCAgcg                                                                          >  1:335634/1‑251 (MQ=255)
                                                                                                                                                                 gCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCAAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGGCAgcggc                                                                        >  1:405789/1‑251 (MQ=255)
                                                                                                                                                                      caccccc‑AGTCCGCGGATTAACTCTTAAAAGG‑TGT‑TT‑AA‑GCCTTGCTCTAGGGCCAGGCAGCCG‑‑TTTTAAAAAA‑G‑CGTACGC‑ATCCCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGGCAGCGGCGATg                                                                    <  1:152799/248‑1 (MQ=255)
                                                                                                                                                                            ccggtcagcgGATTACCTCT‑CAATGG‑TGGGTT‑AA‑GCCTTGCGTGCCGGCCGGGCAGGTG‑‑TTTTAAAAAA‑G‑CTTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGGGCCTGCCAGTGGGGCAGCGGCGATGGCCAt                                                               <  2:63753/244‑1 (MQ=255)
                                                                                                                                                                                tACGCGGATTAACTCTTCAAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGGCAGCGGCGATGGCCATACAt                                                           >  2:121146/1‑251 (MQ=255)
                                                                                                                                                                                    cgGATTAACTCTTCAAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGGCAGCGGCGATGGCCATACATTGTc                                                       >  1:167397/1‑251 (MQ=255)
                                                                                                                                                                                            cctctcaaagggggg‑tT‑AA‑GTCTTGCTCTGCGCCCAGCCAGCTG‑‑TTTAAAAAAA‑G‑CGCACGA‑ACCCCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGGCAGCGGCGATGGCCATACATTGTCTGGAAtg                                                <  1:70399/238‑1 (MQ=255)
                                                                                                                                                                                              cttaaaaGT‑TGG‑TT‑AG‑GCTTTTCTCTCCGGCCAGGCCGCTG‑‑TTTTCAAAAG‑G‑CGTCCGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGGCAGCGGCGATGGCCATACATTGTCTGGAAtgt                                               <  1:362353/245‑1 (MQ=255)
                                                                                                                                                                                                         gg‑tT‑AA‑CCTTTGCTCTACGCCCAGGCGGCCG‑‑TTTAAAAAAA‑G‑CGAACGC‑AACCCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGGCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTccc                                   <  1:499607/251‑1 (MQ=255)
                                                                                                                                                                                                          g‑tT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGGCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTcccc                                  >  2:66063/1‑251 (MQ=255)
                                                                                                                                                                                                           ggtaaa‑GCCTTCCTCTACGGCCAGCCAGCCT‑‑TTTTAAAAAG‑G‑CGTACCC‑ATCCCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGGCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTccc                                   <  1:462790/247‑1 (MQ=255)
                                                                                                                                                                                                               aa‑GCCTTGCTCTCCGCCCAGGCAGCC‑‑‑TTTTCAAAAAAG‑CGGCCGA‑TTCCCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGGCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCtt                                <  1:282798/250‑1 (MQ=255)
                                                                                                                                                                                                                  cccTTGCTCTCCGGCGAGGCACCCG‑‑TTTACAAAAA‑G‑CTTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGGCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTtgct                             <  1:217651/250‑1 (MQ=255)
                                                                                                                                                                                                                      ggCTCTCCGGCCAGGCAGCTG‑‑TTTCAAAAAA‑G‑CGTCCGA‑AACCCCGCCTGTCGTGCGGGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACGTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGGCAGCGGCGATGTCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCg                         <  1:465339/250‑1 (MQ=255)
                                                                                                                                                                                                                       gCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGGCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCgg                        >  1:431285/1‑251 (MQ=255)
                                                                                                                                                                                                                          ctcgggCCGGGCAGCCT‑‑TTTTAAAAAA‑G‑CGTCCGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGGCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGc                       <  1:273720/245‑1 (MQ=255)
                                                                                                                                                                                                                                 ggccagccacccttttCAAAAAAG‑CGTCCGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGGCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGTTGCCGGCGACAAt                 <  1:427611/239‑1 (MQ=255)
                                                                                                                                                                                                                                      aGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGGCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCa          >  1:50263/1‑250 (MQ=255)
                                                                                                                                                                                                                                               ttCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGACATCCGCTGACGAATGCCCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGGCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGGGGGCAAAGTTTGc  >  2:525640/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                           
CGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCATCCCATCTTGCGCATT‑GCC‑GTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCG‑GCATCCCC‑AGTACGCGGATTAACTCTTCAAAGG‑TGG‑TT‑AA‑GCCTTGCTCTACGGCCAGGCAGCCG‑‑TTTTCAAAAA‑G‑CGTACGC‑ATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAATGACCGGCGTTATGGGCAGAACTTCAAATAAGGCCGTACGACCATAAAAACCGTGGTAGCAATGTACACAACCGGGTGCCTGCCAGTGGGGCAGCGGCGATGGCCATACATTGTCTGGAATGTGGATGGGCTCCCCTTGCTGCCGGCGACAATGTGGGCAAAGTTTGC  >  NC_000913/115566‑116045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑