Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,274,9400AT30.0% 53.7 / 10.9 30intergenic (‑33/‑342)garP/garDgalactarate/D‑glucarate transporter GarP/GarD
Reads supporting (aligned to +/- strand):  ref base A (6/15);  new base T (9/0);  total (15/15)
Fisher's exact test for biased strand distribution p-value = 7.00e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGGATTTGCATCAGCAAGTAGGCCCAGCCAAAAGCGGAGAAGATGTAACCCATCGAAACCGCACTTAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTC‑CAGAATCATTTTATTT‑ACCTCGGGTACTTATGCTGATTTTTATTATTAT‑GGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGCACAGATTTGCGCTTTACCTTACCGGGCGGCACTGCAATCCCTGAAATGATTGACATTGATCAC  >  NC_000913/3274693‑3275172
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                         
gggATTTGCATCAGCAAGTAGGCCCAGCCAAAAGCGGAGAAGATGTAACCCATCGAAACCGCACTTAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTC‑CAGAATCATTTTATTT‑ACCTCGGGTACTTATGCTGATTTttatta                                                                                                                                                                                                                                        <  1:443086/251‑1 (MQ=255)
   aTTTGCACCAGCAAGTAGGCCCAGCCAAAAGCGGAGAAGATGTAACCCATCGAAACCGCACTTCACTGCAACTCTTTTGCCACTTCGGTACCAGCACTAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAAAAAAAAAAATAATCACATATATTTTATAGCGGGGGTGCCCGCCCTTTTTTTTTTTGTCAAAGGTGTCGCA‑AATCATTTTTTTT‑ACCTCCGGGAATTCTACTGTTTTttattattt                                                                                                                                                                                                                                     >  2:445507/1‑250 (MQ=255)
                aGTAGGCCCAGCCAAAAGCGGAGAAGATGTAACCCATCGAAACCGCACTTAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTC‑CAGAATCATTTTATTT‑ACCTCGGGTACTTATGCTGATTTTTATTATTAT‑GGGGAAGGTGtt                                                                                                                                                                                                                       <  1:315326/251‑1 (MQ=255)
                              aaaGCGGAGAAGATGTAACCCATCGAAACCGCACTTAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACCATAAAAAAAATCAGGAATATTTAATAGCGGGGGTGCCCGCCTTTCTTTTTTTTGTTAAAGGGGGC‑CCGGATCCTTTTTTTT‑TTCTCGGGTACTTTTTCTGTTTTTTTTTTTTTT‑GGGGGAGGGGTTATTTTTGTGttttt                                                                                                                                                                                                         >  2:340883/1‑249 (MQ=255)
                                   ggAGAAGATGTAACCCATCGAAACCGCACTTAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAAAAAAAAAAATAAACAGTAATATTAAATAGAGGGGATGCACGCCTTTCTTTTTTTTGTCAACAGTGTC‑CAGAAATATTTTTTTT‑ACCTCGGGGACTTTTTTTTATTTTTTTTTTTTT‑TGGGAAGGTGttattttttaggtttatttat                                                                                                                                                                                                    >  2:372208/1‑236 (MQ=255)
                                      gaagaTGTAACCCATCGAAACCGCACTTAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTC‑CAGAATCATTTTATTT‑ACCTCGGGTACTTATGCTGATTTTTATTATTAT‑GGGGAAGGTGTTATTTATGAGTTTCATTTATGcc                                                                                                                                                                                                 <  1:340883/251‑1 (MQ=255)
                                                   aTCGAAACCGCACTTAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCCGTAATATTAAATAGCGGGTATGCACGCCCTTTTTTTTTTTGTCAACGGTGTC‑CAGAATCATTTTATTT‑TCCCCGGGGACTTATTCTGATTTTTTTTTTTAT‑TGGGAAGGGGTTATTTATGAGTTTTCTTTTTGTCGTAACGACAATg                                                                                                                                                                                     >  2:403250/1‑250 (MQ=255)
                                                           cGCACTTAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGGAACCATAAAAATAATCAGTAATATTAAATAGCGGGGATGCACGCCATTTTTTTTTTTGTTAACGGTGTC‑CAGGAACATTTTTTTTTACC‑CGGGGACTTTTTCTGCTTTTTTTTTTTTT‑GGGGGAGGGGTTATTTATTAGTTTCTTTTATTGCGTAACGACAATGAACTCgggg                                                                                                                                                                            >  2:440216/1‑250 (MQ=255)
                                                                 tAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAAAAATAAAAAAAAACAGTAATATTTAATAGCGGGGGTGCACGCCCTTCTTTTTTTTGTCAACGGTGTT‑CAGAATCATTTTATTT‑TCCTCCGGTACTTTTTCTGGTTTTTTTTTTTAT‑GGGGGAGGGGTTTTTTTTGAGTTTCATTTATTCCGTAACGCCAATGAACTCGGGGAtttgt                                                                                                                                                                      >  2:171655/1‑248 (MQ=255)
                                                                          ctctTTTGCCACTTCGGTACCAGCACTAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAAAAAGGTATATTTAAAAGCGGGTGTTCAAGCCCTTTTTTTTTTTGTCCACGGGGGC‑CAGAATAATTTTTTTT‑TCCTCGGGGAATTTTTTTGATTTTTTTTTTTTT‑GGGGGAGGTGTTTTTTATTAGTTTCATTTTTTTCGTTAAGACAATGAACTCGGGAATTTGTATaaacg                                                                                                                                                               >  2:423004/1‑246 (MQ=255)
                                                                               ttGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTC‑CAGAATCATTTTATTT‑ACCTCGGGTACTTATGCTGATTTTTATTATTAT‑GGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGAATTAGtata                                                                                                                                                                   <  2:147415/240‑1 (MQ=255)
                                                                               ttGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAACGGGGATGCACGCCCTTTTTTTTTTCGTCAACGGGGTC‑CAGAATCATTTTTTTT‑TCCCCGGGGACTTTTGCTGGTTTTTTTTTTTTT‑GGGGAAGGGGTTATTTTTGAGTTTCATTTTTTCCGTAACGACAATGAACTCGGGAATTAGtata                                                                                                                                                                   >  1:147415/1‑240 (MQ=255)
                                                                                 gCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTC‑CAGAATCATTTTATTT‑ACCTCGGGTACTTATGCTGATTTTTATTATTAT‑GGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGAATTAGTATAAGCAGCGCGAGaa                                                                                                                                                      <  1:440216/251‑1 (MQ=255)
                                                                                                            gTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATTATTAGTAAAATTTAATAAAGGGGATGCGCGCCTTTTTTTTTTTTGTCAACAGGGGC‑CCGAATCATTTTTTTT‑TACTCGGGTACTTTTGCTGTTTTTTTTTTTTAT‑GGGGGAGGGGTTTTTTTTGAGTTTTTTTTTTTCCGTTACGACAATGAAATCGGGGATTAGTATAAGCAACGCGAGAATAAAAAACATTTTGCCAATGCtaattt                                                                                                                           >  2:107219/1‑251 (MQ=255)
                                                                                                                cACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTC‑CAGAATCATTTTATTT‑ACCTCGGGTACTTATGCTGATTTTTATTATTAT‑GGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGAATTAGTATAATCAGCGCGAGAATAATAATCATTGTGCAAATGCtaatttaatt                                                                                                                       <  1:445507/251‑1 (MQ=255)
                                                                                                                       ggCGTAGTTAACGGCGGTTACAATAAAAAACAACACTAATATTTAATAGCGGGTATGCACGCCTTTCTTTTTTTTTTCAACAGTGTC‑AAGAAATATTTTATTT‑AACTCGGGTACTTTTGTTGTTTTTTTTTATTTTTGGGGAGGTTTTTTTTTTTGGGTTT‑ATTTTTTGCGTCACGACAAAGAAATCTGGAATTATTATAAGAAGAGCGAGGATACAAATCATTGTGCAAACTCTAATttaattacatcat                                                                                                                >  2:143079/1‑245 (MQ=255)
                                                                                                                          gTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTC‑CAGAATCATTTTATTT‑ACCTCGGGTACTTATGCTGATTTTTATTATTAT‑GGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTAttt                                                                                                             <  2:68634/251‑1 (MQ=255)
                                                                                                                                               aaaaaTAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTC‑CAGAATCATTTTATTT‑ACCTCGGGTACTTATGCTGATTTTTATTATTAT‑GGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTAttt                                                                                                             <  2:496314/230‑1 (MQ=255)
                                                                                                                                               aaaaaTAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTC‑CAGAATCATTTTATTT‑ACCTCGGGTACTTATGCTGATTTTTATTATTAT‑GGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTAttt                                                                                                             >  1:496314/1‑230 (MQ=255)
                                                                                                                                               aaaaaTAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTC‑CAGAATCATTTTATTT‑ACCTCGGGTACTTATGCTGATTTTTATTATTAT‑GGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTGGAGCatat                                                                                         <  1:423004/250‑1 (MQ=255)
                                                                                                                                                          gTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTC‑CAGAATCATTTTATTT‑ACCTCGGGTACTTATGCTGATTTTTATTATTAT‑GGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGtt                                                                             <  1:372208/251‑1 (MQ=255)
                                                                                                                                                                  aaaTAGCGGGGATGCACGCCTTTCTTTTTTTCGTCAACGGTGTC‑CAGAATCATTTTATTT‑ACCTCGGGTACTTATGCTGATTTTTATTATTAT‑GGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCt                                                                      >  1:72696/1‑250 (MQ=255)
                                                                                                                                                                                cACGCCTTTCTTTTTTTCGTCAACGGTGTC‑CAGAATCATTTTATTT‑ACCTCGGGTACTTATGCTGATTTTTATTATTAT‑GGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGCACAGATTTgc                                                       >  2:194693/1‑251 (MQ=255)
                                                                                                                                                                                              tttCGTCAACGGTGTC‑CAGAATCATTTTATTT‑ACCTCGGGTACTTATGCTGATTTTTATTATTAT‑GGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGCACAGATTTGCGCTttaccttacc                                          <  1:4865/250‑1 (MQ=255)
                                                                                                                                                                                                    cAACGGTGTC‑CAGAATAATTTAATTT‑ACCTCGGGTACTTATGCTGATTTTTACTATTAT‑GGGGAAGGTCTTATTTATGAGTTTCCTTTATGCCGTAACGACAATGCACTCGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATCTGCACATAAGGTTGCGCGCTAAAGCACAGATTTGCGCTTTCCCTTACCGggcggc                                   >  2:528310/1‑251 (MQ=255)
                                                                                                                                                                                                                   aTCATTTTATTT‑ACCTCGGGTACTTATGCTGATTTTTATTATTAT‑GGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGCACAGATTTGCGCTTTACCTTACCGGGCGGCACTGCAATCCCTg                      >  1:271276/1‑250 (MQ=255)
                                                                                                                                                                                                                         ttattt‑tCCTCGGGTACTTATGCTGATTTTTATTATTAT‑GGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGCACAGATTTGCGCTTTACCTTACCGGGCGGCACTGCAATCCCTGAAATGa                <  2:6261/250‑1 (MQ=255)
                                                                                                                                                                                                                                aCCTCGGGTACTTATGCTGATTTTTATTATTAT‑TGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGAATTAGTAGAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAAGTAATACTATTTAAATATGATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGCACAGATTTGCTCTTTACCTTACCGGGCGGCACTGCAATCCCTGAAATGATTGACAt         <  1:266368/251‑1 (MQ=255)
                                                                                                                                                                                                                                     gggTACTTATGCTGATTTTTATTATTAT‑GGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGCACAGAGTTGCGCTTTACCTTACCGGGCGGCACTGCAATCCCTGCAATGATTGACATTGATc    <  2:72696/251‑1 (MQ=255)
                                                                                                                                                                                                                                      ggTACTTATGCTGATTTTTATTATTAT‑GGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGCACAGATTTGCGCTTTACCTTACCGGGCGGCACTGCAATCCCTg                      >  2:414919/1‑232 (MQ=255)
                                                                                                                                                                                                                                      ggTACTTATGCTGATTTTTATTATTAT‑GGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGCACAGATTTGCGCTTTACCTTACCGGGCGGCACTGCAATCCCTg                      <  1:414919/232‑1 (MQ=255)
                                                                                                                                                                                                                                       gTACTTATGCTGATTTTTATTATTAT‑GGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGCACAGATTTGCGCTTTACCTTACCGGGCGGCACTGCAATCCCTGAAATGATGGACATTGATcac  <  1:438046/251‑1 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                         
GGGATTTGCATCAGCAAGTAGGCCCAGCCAAAAGCGGAGAAGATGTAACCCATCGAAACCGCACTTAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTC‑CAGAATCATTTTATTT‑ACCTCGGGTACTTATGCTGATTTTTATTATTAT‑GGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGAATTAGTATAAGCAGCGCGAGAATAATAATCATTGTGCAAATGCTAATTTAATTAATACTATTTAAATATTATTTTGAGCATATGCACATAAGGTTGCGCGCTAAAGCACAGATTTGCGCTTTACCTTACCGGGCGGCACTGCAATCCCTGAAATGATTGACATTGATCAC  >  NC_000913/3274693‑3275172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑