| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,889,514 | 0 | C | T | 21.1% | 127.2 / 11.3 | 57 | E196K (GAA→AAA) | cysI | sulfite reductase, hemoprotein subunit |
| Reads supporting (aligned to +/- strand): ref base C (16/29); new base T (12/0); total (28/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.30e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGCTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGC‑GTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGTTACGGTTCATGTCGTTAGCTGTCGCCAGCGCATCAAGACCGACCGAGTGCAGCATCTGGTGCACCGGTTTGACGTTCTTTTTCAGAATGCCGTGGAACTGAAACGTCTGGCGGTTGGTCAGGCGA > NC_000913/2889274‑2889764 | aGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGCTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGC‑GTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGc < 1:7793/251‑1 (MQ=255) cactcGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGCTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGC‑GTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTcc < 2:152491/251‑1 (MQ=255) cactcGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGCTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGC‑GTGCAGATCGATATCGTTCGGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTcc < 1:431753/251‑1 (MQ=255) gCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGCTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGC‑GTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGGGGTTTTGAATTTACGCGGCAGGGAGGGCTGGGCGAGGAT‑CGG‑TTCTTTATCAGTAGTGGGG‑GCTTTTTTCTGGTCGAGCCAGATCTCCGCATAcgcgc > 1:363634/1‑251 (MQ=255) gggCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGCTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGC‑GTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTTGATTTTCGCGGCAGGGAGGTCTGGCCGGGGGT‑CGG‑TTCTTCTTCAGTAGTGGCG‑ACTTTTTCCTTGTCGAGGCAGATCTCCGccttagcccgg > 2:159418/1‑241 (MQ=255) ttgCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGCTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGC‑GTGCAGATCGATATCGTTCTGTGGCGGGGTCACTACCGTGGGTTTGTATTTTAGCGGCAGGGAGGGCTGGGCGCGGATTCGG‑TT‑TTTATTTGGTGTGGGGCGCTTTTTC‑TGGTCGCGCCAGATCTCCGCCTACCCGCGGGGACGGGGCAACAGa > 2:321667/1‑249 (MQ=255) gtTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGCTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGC‑GTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGa < 2:469836/250‑1 (MQ=255) ggAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGCTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGC‑GTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAAtcttc < 1:397397/250‑1 (MQ=255) aaaGCCCCCCGCCCACCAACAGGTTAAAGCCCACCAGCTTGCCTTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGC‑GTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCttcttc < 2:476801/251‑1 (MQ=255) cccaccAACAGGTTAAAGCCCACCAGCTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGC‑GTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTCTCCTGGTCGATCCAGATCTCCGCATACGCGCGGGGTCGAGTCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTa < 2:461378/251‑1 (MQ=255) aGGTTAAAGCCCACCAGCTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGC‑GTGCAGATCGATATCGTTCTGTGGCGGGATTCCTACCGCGGTTTTTAATTTACGCGGCAAGGAGGTCTGGGCGAGGGT‑CGG‑TTTTTCAACAGTAATGGCG‑GCTTTTTTCTGGGCGAGCCAAACCTCCGCATACGACCGGGTGCGCGGCAACAAATGCTCCGCAATCTTCTTCccccctccctacgcctccgc > 1:69481/1‑235 (MQ=255) gTTAAAGCCCAACAGCTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGC‑GTGCAGATCGATATCGTTCTGTGGCGGGGTCACTACCGTGGGTTTTAATTTTCGCGGCAGGGAGGGCTGGCCGAGGGT‑CGG‑GTCTTTATCAGTAGTGGGG‑GCTTTTTTCTGGTCGGGCCCGATCTCCCCATACGCGCGGGGACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACCCGTACGCTTCcgccg > 1:141078/1‑249 (MQ=255) tAAAGCCCACCAGCTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGC‑GTGCAGATCGATATCGTTCTGTGGCGGGATCGCTACCGCGGGTTTTAATTTACGCGGGAGGTAGGGCTGGCCGAGGAG‑CGG‑TTCTTCTTTCGGAGAGGCG‑CCTTTTTCCTGCGCTGGCCAGATTCCCCCATACGCCCGGGGACAAGGCAACAGATCCTCCGCAATCTTCTTTGCCCACCCGTACGCTTCCTCGTGc > 1:315009/1‑251 (MQ=255) aaaGCCCCCCAGCTGGCCGTTTGCGGCGATCGCCACGAAGTTCATGTCGTTGGC‑GTGCAGATCGATATCGTGCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCGTCGCCCACTCGTACGCTGCCGCGTGc < 2:390678/250‑1 (MQ=255) aaaGCCCACCAGCTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGC‑GTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGc < 2:469291/250‑1 (MQ=255) ccaccaGCTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGC‑GTGCAGATCGATATCGTTCTGTGGCGGGATCACTCCCGGGGTTTTGAATTTTCGCGGCCGGGAGGTCTGGCCGAGGGT‑CGG‑GTCTTCTTCAGTAGTGGGG‑ACTTTTTTCTGGGCGCGCCAGATATCCGCATAAGCGCGGGGACGAGGGCACAAATACTCAGAAATCTTTTTCTCCCACTCGTACCCTTCCGCGTGCAGCTGc > 1:352634/1‑251 (MQ=255) ttGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGC‑GTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGGGGTTTTGAATTTACGCGGCAGGGAGGTCTGGCCGAGGAT‑CGG‑TTCTTTATCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGGACGAGGCAACAAATGCTCAGAAATCTTCTTCGCCCCCTCGTACGCTTCCGCGGGCAGCTGCGACTCGt > 1:181528/1‑250 (MQ=255) tGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGC‑GTGCAGATCGATATCGTTCTGTGGCGGGAGCCCAACCGGGGTTTTTAATTTTCGCGGCAGGGAGGGCTGGGCGGGGGT‑CGGGTTTTTC‑TCAGGAGTGGGG‑GCTTTTTTCTGGTGGAGCCCGATCTCCGCCTTCTCGCGGGGAGGAGGCAACACATGCTCAGAAATCTTTTTCTCCCCCCCGTACGCCTCCGCGCGCAGCGCCGACTCggtg > 2:59145/1‑248 (MQ=255) gTTTTCGGCGATCGCCCCGAAGTTCATGGCGTTGGC‑GTGCAGAGCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGt < 2:45398/251‑1 (MQ=255) ttgtCGGCGATCGCCACGAAGTGCATGTCGTTGGC‑GTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTTGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGTCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGt < 1:295196/247‑1 (MQ=255) ttCGGCGATCGCCACGAAGTTCATGTCGTTGGC‑GTGCAGATCGATAGCGTTCTGGGGCGGGAGCACGACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGtta < 2:134378/250‑2 (MQ=255) cGGCGATCGCCACGCAGTTCATGTCGTTGGC‑GTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGGAGGGCTGGCCGAGGAT‑CGG‑GTCTTCATCAGTAGTGGCG‑GCTTTTTTCTGGGCGGGCCAGATCTCCCCATAAGCGCGGGGACGAGGCAACAGATGGTCAGAAATCTTCTTCGCCCACTCGTACGCCTCCCCGTGCAGCTGCGCCTCGGAAggggtgg > 1:122337/1‑245 (MQ=255) ggCGATCGCCACGAAGTTCATGTCGTTGGC‑GTGCAGATCGATATCATTCTGTGGCGGGATCACTACCGCGGATTTTTATTTTCTCGGCAGGTAGGCTTGGCCGGGGGT‑CGG‑TTTTTCATCAGTAGTGGCG‑ACTTTTTTCTGGGCGAGACAGATCTCCGCATACGCGCGGGTACCCGGGAACAAATTCTCATAAATCTTCCTTGCCCCCCCGTTCGCTTTCGCGGGGAGTTGCGACTCGTAAAGGTTCgcgg > 1:473925/1‑248 (MQ=255) gCGATCGCCACGAAGTTCATGTCGTTGGC‑GTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGt < 1:425548/251‑1 (MQ=255) cGATCGCCACGAAGTTCATGTCGTTGGC‑GTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTg < 1:159418/251‑1 (MQ=255) gATCGCCACGAAGTTCCTGTCGTTGGC‑GTGCAGATCGATATCGTTCTGTGGCGGGATCACTAACGGGGGTTTGACTTTTCGCGGCAGGGAGGTCTGGCCGAGGAT‑CGG‑TTATTCATCAGGAGTGGCG‑ACTTTTTTCTGGTCGCGCAAAATCTCCGCCTACGCGCGGGGACGAGGCAACAAAAGCTCAGAAAACTTCTTTGCCCACTCGTACGCTTCCCCGGGCAGCTGCGCCTCGTAAGGGGTCGGGGTgg > 1:181939/1‑250 (MQ=255) gCCACGAAGTTCATGTCGTTGGC‑GTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGCGGGTTTTAATTTACGCGGCAGGGAGGTCTGGCCGAGGAT‑CGG‑TTTTTCATCAGTAGGGGCG‑GCTTTTTTCTGGGCGAGCCAGATCTCCGCATACGCGCGGGGAAGCGGCAACAGAAGCTCAGAAATCTTTTTCGCCCACTCCTTCGCTTCCGCGTGCCGCTGCGCCTCGTTAGGGGTCGAgggggaggg > 1:378916/1‑244 (MQ=255) gAAGTTCATGTCGTTGGC‑GTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTTAATTTACGCGGCAGGTAGGTCTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTGGCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTGCGCCCACTCGTACGCGTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGt < 2:187453/251‑1 (MQ=255) gAAGTTCATGTCGTTGGC‑GTGCAGATCGATATCGTTCTGTGGCGGGATCACTAACGTGGTTTTTGATTTACCCGGCAGGGAGGTCTGGCCGGGGAT‑CGG‑GTCTTCCTCAGGAGTGGCG‑GCTTTTTTCCGGTCGAGCCAGATCTCCGCATACGCGCGGGGACGCGGCAAAAAATGCTCAAAAATATTCTTCGCCCACTCGTACGCTTCCGCCGGCAGCTGCGGCCCGCAAGGGTTCGCGGGGCagcgttcgt > 1:410962/1‑244 (MQ=255) gAAGTGCATGTCGTTGGC‑GTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGt < 2:472264/251‑1 (MQ=255) attCATGTCGTTGGCCGGCCAGTCCGATAT‑GTTGTGTGGCCGGATCCCTACCGTGGGTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGTTAc < 2:12908/250‑1 (MQ=255) gTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGGTTTGAATTTACGCGGGCGGTAGGTCTGGCCGAGGAT‑CGG‑GTCTTCATCAGTAGGGGCG‑ACTTTTTTCTGGGCGAGCCCGATCTCCGCATAAGCGCGGGGACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCCCTCGTACGCTTCCGCGTGCCGGTGCGGCTCGGAAGGGTTCGAGGGGCAGAGGAAGTTACGGTTCATGTCGtt > 1:63396/1‑249 (MQ=255) ggCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGTTACGGTTCATGTCGTTAGc < 2:230004/250‑1 (MQ=255) cAGATCGATATCGTTCTGTGGCGGGATCCATACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGCGGGT‑CGG‑GTCTTCATCAGTAGTGGCG‑GCTTTTTTCTGGTCGAGGCCGATCTCCGCCTACGCGCGGGGACGCGGCAACCGATGCTCAGAAAACTTCTTCGCCCCCTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGGTCGGGGGGCAGAGGACGTTACGGGTCAttgcgtttgctg > 1:387021/1‑240 (MQ=255) gATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGTTACGGTTCATGTCGTTAGCTGTc < 1:321667/251‑1 (MQ=255) gATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGTTACGGTTCATGTCGTTAGCTGTc < 1:420959/251‑1 (MQ=255) aTCGATATCGTTCTGTGGCGGGATCACTACCGGGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTTTCCTGGTc < 1:246460/108‑1 (MQ=255) atatCGTGCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGTTACGGTTCATGTCGTGAGCTGTCGCCAg < 2:227605/251‑1 (MQ=255) cTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGTTACGGTTCATGTCGTTAGCTGTCGCCAGCGCATCAAGACCGACCGAGTgc > 1:312735/1‑251 (MQ=255) aCCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGTTACGGTTCATGTCGTTAGCTGTCGCCAGCGCATCAAGCCCGACCGCGTgc > 1:18773/1‑249 (MQ=255) ttttGAATTTACGCGGCAGGTAGGTCTGGCCGAGGAT‑CGG‑TTATTCATCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGTTACGGTTCATGTCGTTAGCTGTCGCCAGCGCATCAAGACCGACCGAGTGCAGCATCTgg > 1:211014/1‑251 (MQ=255) tGAATTTACGCGGCAGGTAGGTCTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGTTACGGTTCATGTCGTTAGCTGTCGCCAGCGCATCAAGACCGACCGAGTGCAGCATCTGGTGc > 1:183307/1‑251 (MQ=255) gAATGTACGCGTCAGGTAGGTCGGTCCGCGGAT‑CGG‑TTCTTCAGCAGTAGTGGCG‑ACTTTCTCCTGTTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTCCGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGTTACGGTTCATTTCGGTAGCTGTCGCCAGCGCATCAAGACCGACCGAGTGCAGCATCTGGTGc < 2:68111/250‑1 (MQ=255) aTTTACGCGGCAGGTAGGTCTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGTTACGGTTCATGTCGTTAGCTGTCGCCAGCGCATCAAGACCGACCGAGTGCAGCATCTGGTGCAcc > 2:435968/1‑251 (MQ=255) aTTTACGCGGCAGGTAGGTCTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTTTCCTGGTAGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGTTACGGTTCATGTCGTTAGCTGTCGCCAGCGCATCAAGACCGACCGAGTGCAGCATCTGGTGCAcc > 1:457329/1‑251 (MQ=255) aTTTACGCGGCAGGTAGGGCTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGGGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGTTACGGTTCATGTCGTTAGCTGTCGCCAGCGCATCAAGACCGACCGAGTGCAGCATCTGGTGCAcc < 2:298901/251‑1 (MQ=255) tACGCGGCAGGTAGGCCTGGCCGAGGAT‑CGG‑TTATTCTTCCGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTCCGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGAACGTTACGGTTCATGTCGTTGGCTGTCGCCAGCGCATCAAGCCCGACCGGGTGCAGAACCTGGTGCCCCgtt > 2:485626/1‑249 (MQ=255) aggtaggtCTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGTTACGGTTCATGTCGTTAGCTGTCGCCAGCGCATCAAGACCGACCGAGTGCAGCATCTGGTGCACCGGTTTGACGtt > 1:125505/1‑251 (MQ=255) aggtaggtCTGGCAGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGTTACGGTTCATGTCGTTAGCTGTCGCCAGCGCATCAAGACCGACCGAGTGCAGCATCTGGTGCACCGGTTTGACGtt > 2:228189/1‑251 (MQ=255) gtaggtCTGGCCGAGGAT‑CGG‑TTCTTCAGCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGTTACGGTTCATGTCGTTAGCTGTCGCCAGCGCATCAAGTGCGACCGAGTGCAGCATCTGGTGCACCGGTTTGACGTTCt < 1:46158/251‑1 (MQ=255) aggtCTGGCCGAAGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGTTACGGTTCATGTCGTTAGCTGTCGCCAGCGCATCAAGACCGACCGAGTGCAGCATCTGGTGCACCGGTTTGACGTTCtt < 2:118994/250‑1 (MQ=255) cTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGTTACGGTTCATGTCGTTAGCTGTCGCCAGCGCATCAAGACCGACCGAGTGCAGCATCTGGTGCACCGGTTTGACGTTCTTTTTc > 1:114680/1‑250 (MQ=255) cGG‑TTCTTCATCAGCAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGTTACGGTTCATGTCGTTAGCTGTCGCCAGCGCATCAAGACCGACCGAGTGCAGCATCTGGTGCACCGGTTTGACGTTCTTTTTCAGAATGCCGTgg > 2:252590/1‑250 (MQ=255) tcatcaGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGTTACGGTTCATGTCGTTAGCTGTCGCCAGCGCATCAAGACCGACCGAGTGCAGCATCTGGTGCACCGGTTTGACGTTCTTTTTCAGAATGCCGTGGAACTGaa < 1:460780/250‑1 (MQ=255) catcaGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGTTACGGTTCATGTCGTTAGCTGTCGCCAGCGCATCAAGACCGACCGAGTGCAGCATCTGGTGCACCGGTTTGACGTTCTTTTTCAGAATGCCGTGGAACTGaaa < 2:363634/250‑1 (MQ=255) aCTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGTTACGGTTCATGTCGTTAGCTGTCGCCAGCGCATCAAGACCGACCGAGTGCAGCATCTGGTGCACCGGTTTGACGTTCTTTTTCAGAATGCCGTGGAACTGAAACGTCTGGCGGTTGGt > 1:433425/1‑251 (MQ=255) ttttCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGTTACGGTTCATGTCGTTAGCTGTCGCCAGCGCATCAAGACCGACCGAGTGCAGCATCTGGTGCACCGGTTTGACGTTCTTTTTCAGAATGCCGTGGAACTGAAACGTCTGGCGGTTGGTc < 1:92666/249‑1 (MQ=255) ccTGGTCGAGAAAGATCTCCGAATACGCGCGGGTACGCGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGAGCAGCTGCGACTCGTAAGGGTTCGCGGTGCAGAGTACGTTACGGTTCATGTCGTTCGCTGCCGCCAGCGCATCAAGACCGACCGAGTGCAGCATCTGGTGCACCGGTTTGACGTTCTTTTTCAGAATGCCGTGGAACTGAAAAGTCTGGCGGTTGGTCAGGCgc > 2:437450/1‑250 (MQ=255) | AGCCAAACTCACTCGCGGTGCGGGCGTAGGTTTTCTTGTTGCCGTGTTCGATGGAAAGCCCACCGCCCACCAACAGGTTAAAGCCCACCAGCTTGCCGTTTTCGGCGATCGCCACGAAGTTCATGTCGTTGGC‑GTGCAGATCGATATCGTTCTGTGGCGGGATCACTACCGTGGTTTTGAATTTACGCGGCAGGTAGGTCTGGCCGAGGAT‑CGG‑TTCTTCATCAGTAGTGGCG‑ACTTTTTCCTGGTCGAGCCAGATCTCCGCATACGCGCGGGTACGAGGCAACAGATGCTCAGAAATCTTCTTCGCCCACTCGTACGCTTCCGCGTGCAGCTGCGACTCGTAAGGGTTCGAGGTGCAGAGTACGTTACGGTTCATGTCGTTAGCTGTCGCCAGCGCATCAAGACCGACCGAGTGCAGCATCTGGTGCACCGGTTTGACGTTCTTTTTCAGAATGCCGTGGAACTGAAACGTCTGGCGGTTGGTCAGGCGA > NC_000913/2889274‑2889764 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |