Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,608,244	0	T	G	27.6%	67.8 / 10.1	60	V443G (GTG→GGG)	hyfF	hydrogenase 4 component F
Reads supporting (aligned to +/- strand):  ref base T (16/26);  new base G (14/2);  total (32/28)
Fisher's exact test for biased strand distribution p-value = 9.76e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGC‑AGGGATGCCGCCCTT‑CAA‑CATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTT‑ATTG‑CTGTTAACGCTGG‑TGCTGGCGGGCC‑TGGTACGG‑ATGGCTGC‑GC‑GGGTGTTAATGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCTGGCGATG‑GGAACGCAT‑ATTCCACA‑ACCTGTCATCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTG  >  NC_000913/2607997‑2608489                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                gTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGC‑AGGGATGCCGCCCTT‑CAA‑CATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTT‑ATTG‑CTGTTAACGCTGG‑TGCTGGCGGGCC‑TGGTACGG‑ATGGGTGC‑GC‑GGGGGtt                                                                                                                                                                                                                                                              >  1:447537/1‑251 (MQ=255) gTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGC‑AGGGATGCCGCCCTT‑CAA‑CATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTT‑ATTG‑CTGTTAACGCTGG‑TGCTGGCGGGCC‑TGGTACGG‑ATGGCTGC‑GC‑GGgtgt                                                                                                                                                                                                                                                               <  1:114687/250‑1 (MQ=255)            ggccGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGC‑AGGGATGCCGCCCTT‑CAA‑CATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTT‑ATTG‑CTGTTAACGCTGG‑TGCTGGCGGGCC‑TGGTACGG‑ATGGCTGC‑GC‑GGGTGTTAATGGCGAAAc                                                                                                                                                                                                                                                   <  1:390508/248‑1 (MQ=255)                ggCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTACGCTGGCGCTGGC‑AGGGATGCCGCCCTT‑CAC‑CATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTT‑ATTG‑CTGTTAACGCTGG‑TGCTGGCGGGCC‑TGGGACGG‑AAGGCTGG‑GCCGGGGGTTA‑TGGCGAAAccccc                                                                                                                                                                                                                                               >  1:301220/1‑248 (MQ=255)                         gATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGC‑AGGGATGCCGCCCTT‑CAA‑CATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTT‑ATTG‑CTGTTAACGCTGG‑TGCTGGCGGGCCCTGG‑ACGG‑GTGGCCGC‑GC‑GGGGGTTTATGGGGGAAACGCCG‑CAgggcgg                                                                                                                                                                                                                                     >  2:403039/1‑247 (MQ=255)                             tcAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGC‑AGGGATGCCGCCCTT‑CAA‑CATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTT‑ATTG‑CTGTTAACGCTGG‑TGCTGGCGGGCC‑TGGTACGG‑GTGGCTGC‑GC‑GGGGGTTTATGGCGAAACCGCCC‑CAGGCCG‑TTaa                                                                                                                                                                                                                                 >  1:109122/1‑250 (MQ=255)                                     gtcTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGGGCGCTGGCGCTGGC‑AGGGCTGCCGCCCTT‑CAA‑CATTTTTCTTAGCGAATTTATGCCCATTACCGCCGGGCTGGCCCGTAATCACCTGCTGATTATCGTCCTGCTGTT‑ATTG‑ATGTTAACACTGG‑TGCTGGCGGGCC‑TGGTAAGG‑AGGGCTGCTGG‑GGGGTTTAATG‑CGAAAACGCCGGCGGGCGG‑TTAACGGGGGTg                                                                                                                                                                                                                         >  1:467007/1‑251 (MQ=255)                                                cccaaaatccatcccttttcccgccgTGCTGTTTGGGGGCGGGGCGCGGGCGCTGGC‑AGGGATGCCGCCCTT‑CAA‑CATTTTTCTTAGCAAATTTATGACCATTCCCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTT‑ATTG‑CTGTTACCGCTGG‑TGCGGGCGGGCC‑TGGTACGG‑ATGGCTGC‑GC‑GGGTGTTAATGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGt                                                                                                                                                                                                           <  1:6907/231‑1 (MQ=255)                                                 caaaaaaccaggccttttcccgcggTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGC‑AGGGATGCCGCCTTT‑CAA‑CTTTTTTCTTAGCAATTTTATGACCTTTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTT‑ATTG‑CTGTTAACGCTGG‑TGTTGGCGGGCC‑GGGTACGG‑ATGGCTGC‑GC‑GGGTGTTAATGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGtt                                                                                                                                                                                                          <  1:327265/232‑1 (MQ=255)                                                   cAAAATCCTGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGC‑AGGGATGCCGCCCTT‑CAA‑CATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTT‑ATTG‑CTGTTAACGCTGG‑TGCTGGCGGGCC‑TGGTACGG‑ATGGGTGC‑GC‑GGGGGTTAATGGGGAAACCGCCG‑CAGGCCG‑TTAACCGGGGGGAATTCGG‑C‑TGGTTgg                                                                                                                                                                                                        >  1:141158/1‑250 (MQ=255)                                                       aTCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGC‑AGGGATGCCGCCCTT‑CAA‑CATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTT‑ATTG‑CTGTTAACGCTGG‑TGCTGGCGGGCC‑TGGTTCGG‑GTGGCTGC‑GC‑GGGGGGTAATGGCGGAACCCCCG‑CCGGCCGGTTAAC‑GGGGTGGTCTCGG‑C‑TGGGTGgcca‑c                                                                                                                                                                                                   >  1:302036/1‑251 (MQ=255)                                                        ccagccttttccccgcgtggtggtgtGGCGGCGGTGCG‑GGGCGGGGGCGAGGGTTGCCGCCCTTACAA‑‑ATTTTTCTTAGGGATTTTATGACCATTCCCGCCGGCCGGGCCCGTAACCCCCTGCGTATTATCGTCCTGTTGTT‑ATTG‑CGTTTAACGTTGG‑TGCGGGCGGGCC‑TGGTACGG‑GTGTCTGC‑GC‑GGTTGTTAGTGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGGGTGATCGCGG‑C‑TGGTTGacca‑cc                                                                                                                                                                                                  <  2:251019/226‑1 (MQ=255)                                                                  tACCGCCGTGCTGTTTGGCGGCGGTGCCCTGGCGCTGGC‑AGGGATGCCGCCCTT‑CAA‑CATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTT‑ATTG‑CTGTTAACGCTGG‑TGCTGGCGGGCC‑CGGTACGG‑GTGGCTGC‑GC‑GGGGGTTAATGGCGGAAACGCCC‑CCGGCCG‑TTAACCGGGGGGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCCATgg                                                                                                                                                                                       >  1:166728/1‑251 (MQ=255)                                                                       ccgTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGC‑AGGGATGCCGCCCTT‑CAA‑CATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTT‑ATTG‑CTGTTAACGCTGG‑TGCTGGCGGGGC‑CGGTACGG‑GTGGCTGCCGC‑GGG‑GTTTATTGCGAAACCCCCC‑CCGGCCG‑TTAAACGGGGGGGTCTCGG‑G‑Tgggtggaccccccccccacggggatt                                                                                                                                                                                    >  2:421155/1‑227 (MQ=255)                                                                           gCTGTTTGGCGGCGGTGCGCTGGCGCTGGC‑AGGGATGCCGCCCTT‑CAA‑CATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGCT‑ATTG‑CTGTTAACGCTGG‑TGCTGGCGGGGC‑TGGTACGG‑ATGGCTTG‑GC‑GGGGGGTAATGGGGGAACCGCCG‑CAGGCCGGTTAAC‑GGGGTGATCTCGG‑G‑CGGGTGGCCA‑CCCC‑GCCAAAGGTggttttgg                                                                                                                                                                              >  1:19269/1‑244 (MQ=255)                                                                                  ggcggcGGTGCGCTGGCGCGGGC‑GGGGATGCCCCCCTT‑AAA‑CATTTTTCTTAGCGAATTTATGACCTTTCCCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTT‑ATTG‑CTGTTACCGCGGG‑TGCTGGCGGGCC‑TGGTACGG‑AGGGCTGC‑GC‑GGGGGTTAATGGGGAAACCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCat                                                                                                                                                                      <  1:354350/251‑1 (MQ=255)                                                                                          ggcgctggcgcggGA‑AGGGATGCCCCCCTT‑CAC‑CTTTTTTTTTAGCAATTTTAGGCCCTTTCCCGCCGGATTGGCACGTAATCCCCTGCTGATTATCGTCCTGCTGTT‑ATTG‑CTGTTACCGCTGG‑TGCTGGCGGGCC‑TGGTACGG‑ATGGCTGC‑GC‑GGGTGTAAATGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCTgg                                                                                                                                                              <  1:486163/250‑1 (MQ=255)                                                                                                           gggATGCCGCCCTT‑CAA‑CATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTT‑ATTG‑CTGTTAACGCTGG‑TGCTGGCGGGCC‑TGGGACGG‑ATGGCTGC‑GC‑GGGGGTTAATGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCTGGCGATG‑GGAACGCat‑at                                                                                                                                            >  1:5907/1‑251 (MQ=255)                                                                                                            gggcgccccccTT‑CAAACATTGTTCTTAGCAAATTTATGACCTTTCCCGCCGGACTGGCACGTAATCCCTTGCTGATTATCTTCCTGTTGTT‑ATTG‑TTTTTAACGCTGG‑TGCTGGCGGGCT‑GGGTACGG‑ATGGTGGC‑GC‑GGGTGTTAATGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGTGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCTGGCGATG‑GGAACGCat‑at                                                                                                                                            <  1:231695/243‑1 (MQ=255)                                                                                                              atcccgccTTT‑CAA‑CATTTTTCTGAGCGATTTTATGACCGTTACCGCCGTGCTGGCACTTAGTGACGTGCTGATTATCGCCCTCTTGTT‑ATTG‑CTGTAAGCGTTGT‑GGGTGGCGGGCG‑TGGGAGGG‑ATGGTTGC‑GG‑GGGTGTTAAGGCCGAGACCGCCG‑CATGCCG‑TGAACCGGGGTGATCTCGG‑C‑TGGGTGACCA‑CCTT‑GCCAATGGTGAGTGTGCTGG‑TCATGATGCTGGCGATG‑GGAACGCAT‑ATTcc                                                                                                                                         <  1:294445/248‑1 (MQ=255)                                                                                                              aTGCCGCCCTT‑CAA‑CATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTT‑ATTG‑CTGTTAACGCTGG‑TGCTGGCGGGCC‑TGGTACGG‑GTTGCCGC‑GC‑GGGGGTTAATGGGGAAACCGCCG‑CCGGGCG‑CTAACCCGGGGGGTCTCGG‑C‑TGGGTTACCA‑CCCC‑GCCCCTGGGGATTCTGCTGG‑GCATGGTGCTGGCGGTG‑GGGACGCAA‑Atttc                                                                                                                                         >  2:363766/1‑249 (MQ=255)                                                                                                                gccgccCTT‑CAA‑CATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTT‑ATTG‑CTGTTAACGCTGG‑TGCTGGCGGGCC‑TGGTACGG‑ATGGCTGC‑GC‑GGGTGTTAATGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGGGGGATCTCGG‑C‑TGGTTGACCC‑CCTCCGCCA‑TGGTGGTTCTGCTGG‑TCATGATGCTGGCGGTG‑GGGAAGCAT‑ATTCcac                                                                                                                                       >  1:286685/1‑251 (MQ=255)                                                                                                                     ccTT‑CAA‑CATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGTTGTT‑ATTG‑CTGTTAACGCTGG‑TGCTGGCGGGCC‑TGGTACGG‑ATGGCTGC‑GC‑GGGTGTTAATGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCTGGCGATG‑GGAACGCAT‑ATTCCACA‑ACCt                                                                                                                                 <  1:374860/251‑1 (MQ=255)                                                                                                                     ccTT‑AAA‑CATTTTGCTGAGCAAATGTATCACCAGTCCCGGCGGACGGGCACGTACGCCCCGGCTGATGAGCGTCCGGCTGTT‑ATTT‑CTGTTACGGCTGG‑TGCGGGCGGGCC‑TGGTGCGG‑ATGGCGGC‑GG‑GGGGGTTAGTGGAGAACCCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCTGGCGATG‑GGAACGCAT‑ATTCCACA‑ACCt                                                                                                                                 <  2:342026/251‑1 (MQ=255)                                                                                                                      cTT‑CAA‑CATTTTTCTTAGCGAATTTATGACCATTACCGCCCGGCTGGCACGTAATCACCTGCTGATTATCGTACTGCTGTT‑ATTG‑CTGTTAACGCTGG‑TGCTGGCGGGCC‑CGGTAAGG‑GAGGCTTC‑GC‑GGGAGTTAATGGCGCAACCGCCG‑CCGGCCG‑TTAAACGGGGTGTTCTCGG‑C‑TGGTTCACAA‑CCTC‑GCCAATGGGGACTCGGCTTG‑GCCTGATGCTGGGGATG‑GGAAAGCAT‑ATTTCACA‑ACCTg                                                                                                                                >  1:65613/1‑251 (MQ=255)                                                                                                                             ccATTTTTCTAAGGGAATTTATTACCTTTCCCGCCGACCTGGCACGTAATCCCCGGCTGATTACCGTCCTGCTGTT‑ATTG‑CTGTTACCGCTGG‑TGCTGGCGGGCC‑GGGTGCGG‑ATGGCTGC‑GC‑GGGTGTTAATGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCTGGCGATG‑GGAACGCAT‑ATTCCACA‑ACCTGtcatc                                                                                                                           <  1:363766/250‑1 (MQ=255)                                                                                                                                tttttCTGAGAGAATTTATGACCTTTCCCGCCGGCCGGCCACGTAACCACCTGCTGATTAGCGTCCTGTTGTT‑‑TTGTCTGTTACCGGGGG‑TGCTGGCGGGCC‑‑GGGGCGGTAGGGCTGC‑GC‑GGGTTTTAAGGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCGGGCGATG‑GGAACGCAT‑ATTCCACA‑ACCTGTCATCAg                                                                                                                         <  2:486557/250‑1 (MQ=255)                                                                                                                                       tAGCGAATTTAGGACCATTCCCGCCGGGCTGGCACGTAATCACCTGCTGTTTTTCGTCCTGTTGTT‑TTTG‑CTGTTAACGCGGG‑TGCTGGGGGGCC‑TGGTACGG‑ATGGCGGC‑GC‑GGGTGTTAATGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCTGGCGATG‑GGAACGCAT‑ATTCCACA‑ACCTGTCATCAGGATCCTg                                                                                                                  <  1:59595/250‑1 (MQ=255)                                                                                                                                        aGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTT‑ATTG‑CTGTTAACGCTGG‑TGCTGGCGGGCC‑TGGGACGG‑ATGGCTGC‑GC‑GGGGGTTAATGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTTGG‑C‑CGGTTTACCA‑CCCC‑GCCAATGGTGATTCTGCTGG‑GCATGATGCTGGCGATG‑GGAACGCAT‑ATTACACA‑ACCTGTCATCAGGATCCTggc                                                                                                                >  1:343051/1‑251 (MQ=255)                                                                                                                                                   gACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTT‑ATTG‑CTGTTAACGCTGG‑TGCTGGCGGGCC‑TGGTACGG‑ATGGCTGC‑GC‑GGGTGTTAATGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGGGGGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCTGGCGATG‑GGGACGCAT‑ATTCCACA‑ACCTGTCATCAGGATCCTGGCGGGCGCTTccc                                                                                                     >  1:164250/1‑250 (MQ=255)                                                                                                                                                          ccccccgGACTGTCACGTAACCACCTGTGGTTTATCGGCGTG‑TGTTTATTG‑CTGTTACCGTTGG‑GGCTGGCGGGC‑‑TGGTCGGGTATGGCGGC‑GG‑GGGTTTTAAGGGCAAACCCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCTGGCGATG‑GGAACGCAT‑ATTCCACA‑ACCTGTCATCAGGATCCTGGCGGGCGCTTCCACTATAGt                                                                                              <  2:272758/247‑1 (MQ=255)                                                                                                                                                          aCCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTT‑ATTG‑CTGTTAACGCTGG‑TGCTGGCGGGCC‑CGGTACGG‑ATGGCTGC‑GC‑GGGTGTTAATGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGGGGGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGGGATTCTGCTGG‑TCATGATGCTGGCGATG‑GGGACGCAT‑ATTCCACA‑ACCTGTCCTCAGGATCCTGGCGGGCGCCTCCAccttagt                                                                                              >  1:403648/1‑245 (MQ=255)                                                                                                                                                            cgccgGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTT‑ATTG‑CTGTTAACGCTGG‑TGCTGGCGGGCC‑TGGTACGG‑ATGGCTGC‑GC‑GGGTGTTAATGGCGAAACCGCCG‑CAGGCCG‑TTTACCGGGGGGATCTCGG‑CCGGGTTG‑CCA‑CCTC‑CCCAATGGGGATTCTGCTGG‑TCCTTATGTTGGCGAGG‑GGAACGCATTATCCCCCA‑CCCTG‑CATCAGGATCCTggggggggcgctccccttattcc                                                                                            >  2:269949/1‑234 (MQ=255)                                                                                                                                                                 gACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTT‑ATTG‑CTGTTAACGCTGG‑TGCTGGCGGGGC‑CGGTACGG‑ATGGCTGC‑GC‑GGGGGTTAATGGGGAAACCGCCG‑CCGGACG‑GTAACCGGGGTGGTCTCGGGC‑GGGTTAACC‑‑CCCC‑GGCCCCGGTGATTCTGCTGGGTCTTG‑TGCTTGCGGTG‑GGGACGCCC‑ATTACACA‑CACTGTCATCAGGACCCTggcgggggggtccccccatgtgcccccc                                                                                       >  2:158591/1‑229 (MQ=255)                                                                                                                                                                      cccagTAATCACCTGCTGTTTACCTTCCTGTTTTT‑ATTG‑CTTTTAGC‑CTGGGTGGGGGCGGGCT‑GGGTACGG‑TTGGCTGC‑GC‑GGGTTGTAAGGGCGAACCCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCGGGCGATG‑GGAACGCAT‑ATTCCACA‑ACCTGTCATCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGAc                                                                                  <  2:186871/247‑1 (MQ=255)                                                                                                                                                                        aCGTAATCACCTGCTGATTATCGTCCTGCTGTT‑ATTG‑CTGTTAACGCTGG‑TGCTGGCGGGCC‑TGGTACGG‑ATGGCTGC‑GC‑GGGTGTTAATGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGGGGGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCCATGGGGATTCTTCTGG‑TCATGATGCTGGCGATG‑GGAACGCAT‑ATTCCCCA‑ACCCTTCATCAGGATCCTGGCGGGCGCTTCCCCTATAATCCTCTCCGGGACGc                                                                                >  1:163507/1‑251 (MQ=255)                                                                                                                                                                          gTAATCACCTGCTGATTATCGTCCTGCTGTT‑ATTG‑CTGTTAACGCTGG‑TGCTGGCGGGCC‑TGGTACCG‑ATGGCTGG‑GC‑GGGTGTTAATGGCGAAAACGCCG‑CAGGCCG‑TTAACCGGGGCGATCTCGG‑A‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCTGGCGATG‑GGAACGCAT‑ATTCCACC‑ACCTGTCCTCAGGATCCTGGCGGGCGCCTCCACCACAGCCCTCTCACGGACGCAc                                                                              >  1:327592/1‑251 (MQ=255)                                                                                                                                                                                    ggtgtttctCGTCCTGCTTTT‑ATTG‑CTTTGAACGCTGG‑TGCGGGGGGGCC‑TGGTACGG‑AGGGCGGC‑GG‑GGGTGTAAAGGGCAAAACCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCTGGCGATG‑GGAACGCAT‑ATTCCACA‑ACCTGTCATCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATctgcc                                                                      <  2:115149/241‑1 (MQ=255)                                                                                                                                                                                       gATTATCGTCCTGCTGTT‑ATTG‑CTGTTAACGCTGG‑TCATGGCGGGCC‑TGGGACGG‑GTGGCTGC‑GC‑GGGTGTCAATGGCGAAACCACCG‑CAGGCCG‑CTAAACGGGGTGATCTTGG‑C‑TAGTTGACAAACCAC‑‑CCAATGGTGATTCCGTTGG‑TCATGAAGATCGCGATGTGGAA‑GCCT‑ATTCC‑CACACCTGTCCTCAGGGTCCTGGCGGGAGCTTCCACTATAGTCCTCTCCAGGACGCACGTACTGGCTGcac                                                                 >  1:283022/1‑251 (MQ=255)                                                                                                                                                                                            tCGTCCTGCTGTT‑ATTG‑CTGTTAACGCTGG‑TGCTGGCGGGCC‑TGGTACGG‑ATGGCTGC‑GC‑GGGTGTTAATGGCGAAAACGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGGTGACCA‑CCCC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCTGGCGATG‑GGAACGCAT‑ATTCCCCC‑ACCTGGCATCAGGATCCTGGCGGGGGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCCCAACgg                                                            >  1:172119/1‑250 (MQ=255)                                                                                                                                                                                              ccgtgtgccctcccgatcTGTTAACGCGGG‑TGCTGGCGGGCC‑TGGTACGG‑AGGGCTGC‑GC‑GGGTGTTAAGGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATGCTGCTGG‑TCATGATGCTGGCGATG‑GGAACGCAT‑ATTCCACA‑ACCTGTCATCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGt                                                            <  1:410348/234‑1 (MQ=255)                                                                                                                                                                                                     gttt‑ATGG‑CTGTTCACGTTGG‑TGCTGGGGGCCC‑GGGTACGG‑GTGTCTGC‑GC‑GGGTGTTAATGGCAAATCCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCTGGCGATG‑GGAACGCAT‑ATTCCACA‑ACCTGTCATCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTg                                                    <  2:19269/248‑1 (MQ=255)                                                                                                                                                                                                            gg‑CTGTTAACGCTGG‑TGCTGGCGGGCC‑TGGTACGG‑ATGGCTGC‑GC‑GGGTGTTAATGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCTGGCGATG‑GGAACGCAT‑ATTCCACA‑ACCTGTCATCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGAt                                             <  1:142319/250‑1 (MQ=255)                                                                                                                                                                                                              ggatctaaacgcgggggcgggggggCC‑GGGTACGG‑AGGGCTGC‑GG‑GGGTGTTAATGCCGAACCCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCTGGCGATG‑GGAACGCAT‑ATTCCACA‑ACCTGTCATCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGAt                                             <  2:18354/229‑1 (MQ=255)                                                                                                                                                                                                                   tAACGCTGG‑TGCTGGCGGGCC‑TGGTACGG‑ATGGCTGC‑GC‑GGGTGTTCATGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGGGCGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCCATGGTGATTCTACCGG‑TCATGATGCTGGCGATG‑GGAACGCAT‑ATTCCACA‑ACCTGTCATCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGAttttttt                                       >  1:186494/1‑250 (MQ=255)                                                                                                                                                                                                                   tAACGCTGG‑TGCTGGCGGGCC‑TGGTACGG‑ATGGCTGC‑GC‑GGGGGTTAATGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGGGATTCTGCTGG‑TCATGATGCTGGCGATG‑GGAACGCAT‑ATTCCACA‑ACCTGTCATCAGGATCCTGGCGGGCGCTTCCCCTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGAt                                             >  1:18354/1‑245 (MQ=255)                                                                                                                                                                                                                          gg‑TGCTGGCGGGCC‑TGGGACGG‑ATGGCTGC‑GC‑GGGTGTTAATGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCTGGCGATG‑GGAACGCAT‑ATTCCACA‑ACCTGTCATCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAg                                <  1:149019/251‑1 (MQ=255)                                                                                                                                                                                                                           g‑TGCGGGCGGGCC‑GGGTACGG‑ATGGTGGC‑GG‑GGGTGTTAATGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCTGGCGATG‑GGAACGCAT‑ATTCCACA‑ACCTGTCATCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAgg                               <  1:162541/251‑1 (MQ=255)                                                                                                                                                                                                                                    gggCC‑TGGTACGG‑ATGGCTGC‑GC‑GGGTGTTAATGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCat                                                                                                                                                                      >  2:140162/1‑111 (MQ=255)                                                                                                                                                                                                                                    gggCC‑TGGGACGG‑ATGGCTGC‑GC‑GGGTGTTAATGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCTAATGGTGATGCTGCTGG‑TCat                                                                                                                                                                      <  1:140162/111‑1 (MQ=255)                                                                                                                                                                                                                                           gttACGG‑AGGGCTGC‑GG‑GGGTTTTAATGGGGAAACCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCTGGCGATT‑GGAACGCAT‑ATTCCACA‑ACCTGTCATCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCGGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGt                   <  1:306939/247‑1 (MQ=255)                                                                                                                                                                                                                                             tACGG‑ATGGCTGC‑GC‑GGGTGTTAATGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCTGGCGATG‑GGAACGCAT‑ATTCCACA‑ACCTGTCATCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCCTCTGTTTCg               >  1:104526/1‑251 (MQ=255)                                                                                                                                                                                                                                              ggtgcggtgggcggcgggggTGTAAGTGGCGAAGCCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCTGGCGATG‑GGAACGCAT‑ATTCCACA‑ACCTGTCATCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGttt                 <  2:166728/234‑1 (MQ=255)                                                                                                                                                                                                                                                   aTGGCTGC‑GC‑GGGTGTTAAGGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCTGGCGATG‑GGAACGCAT‑ATTCCACA‑ACCTGTCATCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGaa          <  1:48815/251‑1 (MQ=255)                                                                                                                                                                                                                                                     ggCTGC‑GC‑GGGTGTTAATGGCGAAACCGCCG‑CCGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCTGGCGCTG‑GGAACGCAT‑ATTCCACA‑ACCTGTCATCAGGATCCTGGCGGGCGCTTCCACTTTAGTCCTCTCAGGGACGCAAGTCCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAac        >  2:422687/1‑251 (MQ=255)                                                                                                                                                                                                                                                       cggc‑gc‑GGTTGTAAATGGCAAAACCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCTGGCGATG‑GGAACGCAT‑ATTCCACA‑ACCTGTCATCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAacac      <  1:122400/249‑1 (MQ=255)                                                                                                                                                                                                                                                       cTGC‑GC‑GGGTGTTAATGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCTGGCGATG‑GGAACGCAT‑ATTCCACA‑ACCTGTCATCAGGATCCTGGCGGGCGATTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGCAacac      >  1:446821/1‑251 (MQ=255)                                                                                                                                                                                                                                                          c‑gc‑gGGTGTTAATGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCTGGCGATG‑GGAACGCAT‑ATTCCACA‑ACCTGTCATCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGt   >  1:378342/1‑251 (MQ=255)                                                                                                                                                                                                                                                          c‑gc‑gGGTGTTAATGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCTGGCGATG‑GGAACGCAT‑ATTCCACA‑ACCTGTCATCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTGTTTGCCTTCAGGCACCGCATCTGGTTCGGAGAAACACAGt   <  1:422687/251‑1 (MQ=255)                                                                                                                                                                                                                                                            gc‑gGGTGTTCATGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCTGGCGATG‑GGAACGCAT‑ATTCCACA‑ACCTGTCATCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTg  >  1:104949/1‑251 (MQ=255)                                                                                                                                                                                                                                                                ggTGTTAATGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCTGGCGATG‑GGAACGCAT‑ATTCCACA‑ACCTGtca                                                                                                                             >  1:303368/1‑125 (MQ=255)                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                GTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGC‑AGGGATGCCGCCCTT‑CAA‑CATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTT‑ATTG‑CTGTTAACGCTGG‑TGCTGGCGGGCC‑TGGTACGG‑ATGGCTGC‑GC‑GGGTGTTAATGGCGAAACCGCCG‑CAGGCCG‑TTAACCGGGGTGATCTCGG‑C‑TGGTTGACCA‑CCTC‑GCCAATGGTGATTCTGCTGG‑TCATGATGCTGGCGATG‑GGAACGCAT‑ATTCCACA‑ACCTGTCATCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTG  >  NC_000913/2607997‑2608489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑