Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913803,8690GT35.3% 37.4 / 14.0 34V123L (GTG→TTG) ybhJputative hydratase YbhJ
Reads supporting (aligned to +/- strand):  ref base G (2/20);  new base T (12/0);  total (14/20)
Fisher's exact test for biased strand distribution p-value = 1.66e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CTCTTCGCATAATACGTCCGGAAATATGGATAAACTTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGG‑TATTTTTGTGCCTCCGC‑ATATTGCGGTCATCC‑ATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACACCC‑G‑TTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACACCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGC  >  NC_000913/803628‑804113
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                        
ctctTAGCATAATACGTCTTGAAATATGGATCGACTTAAAGTTAAGTTTGATTCATTAGCCTCTCACGCTATTACCTTTATTGGTCTTGTACAGACCGCTAAAGCGTCCGGTATGGATCGTTTCCCGCTGCCGCATGTGCTGACCAACTGCCCTAACTCACTCTGCGCCGTCGGCGGAACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGAGGCCCAGCGTTATGGCGG‑CGTTTTtgttgttctcc                                                                                                                                                                                                                                                 >  2:469112/1‑243 (MQ=255)
    gcGCAAAATACGTCCGGAAATATGGATAAACTTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTGATGGCGG‑GATTTTGGTGCCTCCGC‑at                                                                                                                                                                                                                                              <  2:214090/248‑1 (MQ=255)
     cGCATAATACGTCCGGAAATATGGATAAACTTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTAACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGG‑TATTTTTGTGCCTCCGC‑ATAtt                                                                                                                                                                                                                                           <  2:15709/251‑1 (MQ=255)
           ataCGTCCGGAAATATGGATAAACTTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGG‑TATTTTTGTGCCTCCGC‑ATATTGCGGTc                                                                                                                                                                                                                                     <  1:442747/251‑1 (MQ=255)
              cGTCCGGAAATATGGATAAACTTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGG‑TATTTTTGTGCCTCCGC‑ATATTGCGGTcatc                                                                                                                                                                                                                                  <  1:182123/251‑1 (MQ=255)
                 ccGGAAATATTGATAAACGTAAAATTAAGATTGATTCATTAGCCTTTCCCGATATTCCCTTTGTTGGTATGGGACAGACCGCTAAAGCGCCCGGTATGTAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGGTGAGCCGCGGCCCAGCGTTATGGCGG‑TATTTCTGTTCCTCCGC‑ATATTGCGGTcatcc‑at                                                                                                                                                                                                                              <  2:459473/251‑1 (MQ=255)
                  cGGAAAGATGGAAAAACTGAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGCCCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCCCTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGG‑TATTTTTGTGCCTCCGC‑ATATTGCGGTcatcc‑atc                                                                                                                                                                                                                             <  2:232618/251‑1 (MQ=255)
                         atGGATAAACTTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGG‑GATTTTTTTGCCTCCCC‑ATATTGCGGTCATCC‑CTCAatatat                                                                                                                                                                                                                      >  1:452934/1‑251 (MQ=255)
                           ggATAAACTTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCTGCCCAGCGTTATGGCGG‑TATTTTTGTGCCTCCGC‑ATATTGCGGTTATCC‑ATCAATATATGc                                                                                                                                                                                                                    <  1:92816/251‑1 (MQ=255)
                                        aTTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGG‑GATTTTTTTGCCTCCCC‑ATATTTCGGGCATCC‑ATCCATATATTCGTggggtggtggg                                                                                                                                                                                                       >  1:339497/1‑241 (MQ=255)
                                             gTTTGATTCATTAGCCTCTCCCGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGG‑TGTTTTTTTGCCTCCGC‑ACATTTCGGGTAACC‑ATCAataaattcggggggtgatggaagggg                                                                                                                                                                                                  >  1:115796/1‑228 (MQ=255)
                                                tGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGCTATGGCGG‑GATTTTTTTGCCTCCCC‑CTATTTCGGCCATCC‑ATCAatatattggtgtggtgattggaggagggg                                                                                                                                                                                               >  1:464424/1‑228 (MQ=255)
                                                                     tatTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGG‑TATTTTTTTGCCTCCCC‑ATATTTCGGGCCTCC‑CTCAAAATATGCGGGGGGTGATGGCGGGCGGCGGGAAAATTATCCTccgggc                                                                                                                                                                            >  1:373614/1‑244 (MQ=255)
                                                                               gttgGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGCCCACCTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGG‑TATTTTTGTGCCTCCGC‑ATATTGCGGTCATCC‑ATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCacac                                                                                                                                                                <  2:276375/251‑1 (MQ=255)
                                                                                      ttGTACAGACCGCTAAAGGGCCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCGGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGG‑TATTTTTGTGCCTCCGC‑ATATTGCGGTCATCC‑ATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACACCC‑G‑TTa                                                                                                                                                        <  2:263868/250‑1 (MQ=255)
                                                                                           cAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGG‑GATTTTTTTTCCTCCCC‑ATATTTCGGTCATCC‑ATCAATATATTCGTGGGGTGATTGGAGGCGGCGGCAAAATTATCCCCGGGGCAGACAACCACcccccctacggggc                                                                                                                                                    >  1:174667/1‑241 (MQ=255)
                                                                                             gACCGCAAAGGCGTCCGTTATGGACCGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGG‑TATTTTTGTGCCTCCGC‑ATATTGCGGTCATCC‑ATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACACCC‑G‑TTACGGTGCAt                                                                                                                                                <  2:371521/251‑1 (MQ=255)
                                                                                                            cGGTATGGACCGTTTCCCGCTGCCTTATGTGCTGACCAGCTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGG‑TATTTTTGTGCCTCCGC‑ATATTGCGGTCATCC‑ATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACACCC‑G‑TTACGGTTCTTTAGGGACAATGGCAg                                                                                                                                 <  2:214966/251‑1 (MQ=255)
                                                                                                            cGGTATGGAACTTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGG‑TATTTTTGTGCCTCCGC‑ATATTGCGGTCATCC‑ATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACACCC‑G‑TTACGGTGCATTAGGGACAATGGCAg                                                                                                                                 <  2:452934/251‑1 (MQ=255)
                                                                                                                                tGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGAGGCCATATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGTTTTTTTTTGCCCCCCC‑A‑ATTTCGGGCATCCCATCA‑TATATGCCTGAGATGAAGGAAGGGGGCGGCAAAAAGATCCTCGGGGCAGGCAAACAAACCC‑C‑TTACCGGGCATTAGGGAGAATgggcggcggggaggggggcgggggg                                                                                                             >  1:305272/1‑228 (MQ=255)
                                                                                                                                  ccGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGG‑TATTTTTGTGCCTCCGC‑ATATTGCGGTCATCC‑ATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACACCC‑G‑TGACGGTGCATTAGGGACAATTGCAGTCGGTGAGGGCGGCGGTGAGtt                                                                                                           <  1:390567/251‑1 (MQ=255)
                                                                                                                                   cGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGG‑TATTTTTGTGCCTCCGC‑ATATTGCGGTCATCC‑ATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACACCC‑G‑TTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTg                                                                                                          <  1:161314/251‑1 (MQ=255)
                                                                                                                                    gTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGG‑TATTTTTTTGCCTCCGC‑ATATTGCGGGCATCC‑ATCAATATATTCGGGGGATGTTGGGAGGGGGCGGCAAAATGATCCTCCGGGCAGAAAACCCCAACC‑G‑GTACGGGGGCTTTGGGGCAATGGCAGTCGGGGGGGGgggcgggggggtgg                                                                                                         >  1:138337/1‑243 (MQ=255)
                                                                                                                                        gtgCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGG‑TATTTTTTTGGCTCCGC‑CTATTTCGGGCATCC‑CTCAATATATGCGTGAGAAGGTGGCAGGGGGCGGCCAAAAGATTCCCCGGCCAGGCAGCCACACCC‑GCTAACGG‑GCATTTGGGGCAAAGGCAGGCGGGGAggggggggggggggttggga                                                                                                      >  1:50546/1‑233 (MQ=255)
                                                                                                                                                      ccATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGG‑TATTTTTGTGCCTCCGC‑ATATTGCGGTCATCC‑ATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACACCC‑G‑TTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATg                                                                                       <  1:150161/251‑1 (MQ=255)
                                                                                                                                                          aaCTCCCTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGG‑TATTTTTGTGCCTCCGC‑ATATTGCGGTCATCC‑ATCAATATATGCTGGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACACCC‑G‑TTACGGTGCATGAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAGCAGCTGCTTAAGGacac                                                                                   <  2:147016/251‑1 (MQ=255)
                                                                                                                                                          aaCTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGG‑TATTTTTGTGCCTCCGC‑ATATTGCGGTCATCC‑ATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACACCC‑G‑TTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGaca                                                                                    <  2:339497/250‑1 (MQ=255)
                                                                                                                                                              cactcTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGG‑TATTTTTGTGCCTCCGC‑ATATTGCGGTCATCC‑ATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACACCC‑G‑TTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACACCt                                                                                 <  1:346782/249‑1 (MQ=255)
                                                                                                                                                                            ggcggcACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGG‑TATTTTTGTGGCTCCGC‑ATATTTCGGTCATCC‑ATCAATATATTGGTGGGATGATGGGAGGCGGGGGCAAAAAGATCCTCGGGGCAGAAAGCCACACCC‑G‑TTACGGGGCATTTGGGACAAAGGGAGTCGGGGGGGGCGGGGGGGGGTTGGGAAAAAAGCTGGTTTATGAAACCTGGGGTATCggatatcc                                                                 >  1:92378/1‑244 (MQ=255)
                                                                                                                                                                                            gTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGG‑TATTTTTTTGCCCCCCC‑CTATTTCGGGCCTCC‑CTCAATATATGCGGGAGATGATGGGAGGCGGCGGCAAAATGATCCCCGGGGCAAAAAGCCCCCCCCCG‑TT‑CGGGGCCTTTGGGACAATGGCCGGCGGGGGGGGGGGCGGGGGGTTGGTAAAACAGCTGCTTTATGACACCTGGGGGATCGACTATCCGGGCGTGGGTGCgggg                                                 >  1:404571/1‑249 (MQ=255)
                                                                                                                                                                                                         ttttGGTTTATCGGCGGCCCAGCGTTATGGCGG‑TATTTTTTTGCCTCCGCCAT‑TTGCGGTCCTCC‑CTCAATATATGCGTGAGATGCTGGGCGGCGGCGGGAAAATTATCCCCGGGGCAGACAACCCCCCCC‑G‑CTACGGGGGATTTGGGGCACAGGCAGGCGGGGAGGGGGGCGGTGAGTTGGGAAAAAAGCTGCTTTATGACACCTGGGGGATCGACTTTCCCGGCGTGGTTgggggggctcttaccgga                                     >  1:287899/1‑233 (MQ=255)
                                                                                                                                                                                                                 tATCGGCGGCCCAGCGTTATGGCGG‑TATTTTTTTGCCTCCCC‑ACATTGCGGGCATCC‑ATCAATAAATGCGTGGgatgat                                                                                                                                                                                                          >  1:182280/1‑79 (MQ=255)
                                                                                                                                                                                                                 tATCGGCGGCCCAGCGTTATGGCGG‑TATTTTTGTGCCTCCGC‑ATATTGCGGTCATCC‑ATCAATATATGCGTGAgatgat                                                                                                                                                                                                          <  2:182280/79‑1 (MQ=255)
                                                                                                                                                                                                                        ggcCCAGCGTTATGGCGG‑TATTTTTGTGCCTCCGC‑ATATTGCGGGCATCC‑ATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCCCACCC‑G‑TTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGGGAGGTGGGAAAACAGCTGCTTAATGACACCTGGGGTATCGACTATCCGGGCGTGGTTGCGGGGCATCTGACCGGGAAACCTGCGCCGTAtt                     >  1:177308/1‑250 (MQ=255)
                                                                                                                                                                                                                                            aTTTTTGTGCCTCCGC‑ATATTGCGGTCATCC‑ATCAATATATGCGTGAGAGGATGGCAGGCGGCGGCAAAATGATCCTCGGGGCAGACAGCCACACCC‑G‑TTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTTAGTTGGTAAAACAGCTGCTTAATGACACCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTCAGCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGgc  <  1:298216/251‑1 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                        
CTCTTCGCATAATACGTCCGGAAATATGGATAAACTTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGG‑TATTTTTGTGCCTCCGC‑ATATTGCGGTCATCC‑ATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACACCC‑G‑TTACGGTGCATTAGGGACAATGGCAGTCGGTGAGGGCGGCGGTGAGTTGGTAAAACAGCTGCTTAATGACACCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGC  >  NC_000913/803628‑804113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑